Restriction map for
pFN21AB9595
(Linear) (Six-base) MAPSORT of: KIBB9595.seq Check: 7820 from: 1 to: 1659
KIBB9595.orf
With 182 enzymes: SgfI *
September 8, 2014 17:51 ..
AatII G_ACGT'C
Cuts at: 0 946 1659
Size: 946 713
AccI GT'mk_AC
Cuts at: 0 1640 1659
Size: 1640 19
Acc65I G'GTAC_C
Cuts at: 0 1418 1659
Size: 1418 241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 906 1659
Size: 906 753
AflIII A'CryG_T
Cuts at: 0 479 1659
Size: 479 1180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 517 1659
Size: 517 1142
AlwNI CAG_nnn'CTG
Cuts at: 0 452 717 1614 1659
Size: 452 265 897 45
Fragments arranged by size:
897 452 265 45
ApaI G_GGCC'C
Cuts at: 0 197 1659
Size: 197 1462
AscI GG'CGCG_CC
Cuts at: 0 1241 1659
Size: 1241 418
AvaI C'yCGr_G
Cuts at: 0 168 819 1119 1246 1491 1659
Size: 168 651 300 127 245 168
Fragments arranged by size:
651 300 245 168 168 127
BanI G'GyrC_C
Cuts at: 0 45 414 759 1051 1165 1418 1659
Size: 45 369 345 292 114 253 241
Fragments arranged by size:
369 345 292 253 241 114 45
BanII G_rGCy'C
Cuts at: 0 197 778 983 1075 1659
Size: 197 581 205 92 584
Fragments arranged by size:
584 581 205 197 92
BbeI G_GCGC'C
Cuts at: 0 1055 1169 1659
Size: 1055 114 490
Fragments arranged by size:
1055 490 114
BglI GCCn_nnn'nGGC
Cuts at: 0 94 121 821 1006 1659
Size: 94 27 700 185 653
Fragments arranged by size:
700 653 185 94 27
BlpI GC'TnA_GC
Cuts at: 0 847 982 1659
Size: 847 135 677
Fragments arranged by size:
847 677 135
Bme1580I G_kGCm'C
Cuts at: 0 50 197 700 764 1659
Size: 50 147 503 64 895
Fragments arranged by size:
895 503 147 64 50
BmgBI CAC'GTC
Cuts at: 0 1625 1659
Size: 1625 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1002 1659
Size: 1002 657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1089 1547 1659
Size: 1089 458 112
Fragments arranged by size:
1089 458 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1659
Size: 556 1103
BsaI GGTCTCn'nnnn_
Cuts at: 0 1433 1659
Size: 1433 226
BsaAI yAC'GTr
Cuts at: 0 695 1659
Size: 695 964
BsaHI Gr'CG_yC
Cuts at: 0 943 1052 1166 1659
Size: 943 109 114 493
Fragments arranged by size:
943 493 114 109
BsaWI w'CCGG_w
Cuts at: 0 1302 1627 1659
Size: 1302 325 32
Fragments arranged by size:
1302 325 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 454 484 1622 1652 1659
Size: 454 30 1138 30 7
Fragments arranged by size:
1138 454 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 565 1659
Size: 565 1094
BseYI C'CCAG_C
Cuts at: 0 710 1659
Size: 710 949
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 1659
Size: 550 1109
BsiEI CG_ry'CG
Cuts at: 0 89 98 594 728 844 974 1010 1214
Size: 89 9 496 134 116 130 36 204
Cuts at: 1214 1268 1481 1659
Size: 54 213 178
Fragments arranged by size:
496 213 204 178 134 130 116 89
54 36 9
BsiHKAI G_wGCw'C
Cuts at: 0 983 1075 1659
Size: 983 92 584
Fragments arranged by size:
983 584 92
Bsp1286I G_dGCh'C
Cuts at: 0 50 197 700 764 778 983 1075 1659
Size: 50 147 503 64 14 205 92 584
Fragments arranged by size:
584 503 205 147 92 64 50 14
BspEI T'CCGG_A
Cuts at: 0 1627 1659
Size: 1627 32
BspHI T'CATG_A
Cuts at: 0 938 1659
Size: 938 721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1196 1659
Size: 1196 463
BsrBI CCG'CTC
Cuts at: 0 846 1659
Size: 846 813
BsrFI r'CCGG_y
Cuts at: 0 120 1213 1375 1659
Size: 120 1093 162 284
Fragments arranged by size:
1093 284 162 120
BssHII G'CGCG_C
Cuts at: 0 7 1020 1241 1659
Size: 7 1013 221 418
Fragments arranged by size:
1013 418 221 7
BssSI C'ACGA_G
Cuts at: 0 1413 1659
Size: 1413 246
BstEII G'GTnAC_C
Cuts at: 0 1305 1659
Size: 1305 354
BstXI CCAn_nnnn'nTGG
Cuts at: 0 36 225 1659
Size: 36 189 1434
Fragments arranged by size:
1434 189 36
BtgI C'CryG_G
Cuts at: 0 89 263 755 974 1148 1268 1659
Size: 89 174 492 219 174 120 391
Fragments arranged by size:
492 391 219 174 174 120 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 234 1659
Size: 234 1425
BtsI GCAGTG_nn'
Cuts at: 0 1179 1659
Size: 1179 480
DraIII CAC_nnn'GTG
Cuts at: 0 1415 1518 1659
Size: 1415 103 141
Fragments arranged by size:
1415 141 103
EaeI y'GGCC_r
Cuts at: 0 86 95 266 591 725 841 971 1007
Size: 86 9 171 325 134 116 130 36
Cuts at: 1007 1065 1211 1265 1377 1478 1659
Size: 58 146 54 112 101 181
Fragments arranged by size:
325 181 171 146 134 130 116 112
101 86 58 54 36 9
EagI C'GGCC_G
Cuts at: 0 86 95 591 725 841 971 1007 1211
Size: 86 9 496 134 116 130 36 204
Cuts at: 1211 1265 1478 1659
Size: 54 213 181
Fragments arranged by size:
496 213 204 181 134 130 116 86
54 36 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 367 882 1451 1659
Size: 367 515 569 208
Fragments arranged by size:
569 515 367 208
EcoICRI GAG'CTC
Cuts at: 0 981 1073 1659
Size: 981 92 586
Fragments arranged by size:
981 586 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 906 1089 1547 1659
Size: 906 183 458 112
Fragments arranged by size:
906 458 183 112
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1426 1659
Size: 1426 233
EcoO109I rG'GnC_Cy
Cuts at: 0 194 1172 1659
Size: 194 978 487
Fragments arranged by size:
978 487 194
FseI GG_CCGG'CC
Cuts at: 0 1379 1659
Size: 1379 280
FspI TGC'GCA
Cuts at: 0 152 664 1659
Size: 152 512 995
Fragments arranged by size:
995 512 152
FspAI rTGC'GCAy
Cuts at: 0 664 1659
Size: 664 995
HaeII r_GCGC'y
Cuts at: 0 519 752 1048 1055 1169 1659
Size: 519 233 296 7 114 490
Fragments arranged by size:
519 490 296 233 114 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 1622 1654 1659
Size: 454 32 1136 32 5
Fragments arranged by size:
1136 454 32 32 5
KasI G'GCGC_C
Cuts at: 0 1051 1165 1659
Size: 1051 114 494
Fragments arranged by size:
1051 494 114
KpnI G_GTAC'C
Cuts at: 0 1422 1659
Size: 1422 237
MscI TGG'CCA
Cuts at: 0 268 1659
Size: 268 1391
MslI CAynn'nnrTG
Cuts at: 0 115 133 262 1659
Size: 115 18 129 1397
Fragments arranged by size:
1397 129 115 18
MspA1I CmG'CkG
Cuts at: 0 91 743 757 806 976 1041 1150 1659
Size: 91 652 14 49 170 65 109 509
Fragments arranged by size:
652 509 170 109 91 65 49 14
NaeI GCC'GGC
Cuts at: 0 122 1215 1377 1659
Size: 122 1093 162 282
Fragments arranged by size:
1093 282 162 122
NarI GG'CG_CC
Cuts at: 0 1052 1166 1659
Size: 1052 114 493
Fragments arranged by size:
1052 493 114
NcoI C'CATG_G
Cuts at: 0 263 1659
Size: 263 1396
NgoMIV G'CCGG_C
Cuts at: 0 120 1213 1375 1659
Size: 120 1093 162 284
Fragments arranged by size:
1093 284 162 120
NotI GC'GGCC_GC
Cuts at: 0 86 841 971 1007 1478 1659
Size: 86 755 130 36 471 181
Fragments arranged by size:
755 471 181 130 86 36
NspI r_CATG'y
Cuts at: 0 483 1659
Size: 483 1176
PasI CC'CwG_GG
Cuts at: 0 36 735 1659
Size: 36 699 924
Fragments arranged by size:
924 699 36
PciI A'CATG_T
Cuts at: 0 479 1659
Size: 479 1180
PfoI T'CCnGG_A
Cuts at: 0 668 1659
Size: 668 991
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1498 1530 1659
Size: 1498 32 129
Fragments arranged by size:
1498 129 32
PpuMI rG'GwC_Cy
Cuts at: 0 1172 1659
Size: 1172 487
PspOMI G'GGCC_C
Cuts at: 0 193 1659
Size: 193 1466
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 295 327 1659
Size: 295 32 1332
Fragments arranged by size:
1332 295 32
PstI C_TGCA'G
Cuts at: 0 1096 1659
Size: 1096 563
RsrII CG'GwC_CG
Cuts at: 0 643 1659
Size: 643 1016
SacI G_AGCT'C
Cuts at: 0 983 1075 1659
Size: 983 92 584
Fragments arranged by size:
983 584 92
SacII CC_GC'GG
Cuts at: 0 92 758 977 1151 1659
Size: 92 666 219 174 508
Fragments arranged by size:
666 508 219 174 92
SfcI C'TryA_G
Cuts at: 0 240 1080 1092 1647 1659
Size: 240 840 12 555 12
Fragments arranged by size:
840 555 240 12 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 94 1006 1659
Size: 94 912 653
Fragments arranged by size:
912 653 94
SfoI GGC'GCC
Cuts at: 0 1053 1167 1659
Size: 1053 114 492
Fragments arranged by size:
1053 492 114
SmaI CCC'GGG
Cuts at: 0 170 821 1248 1493 1659
Size: 170 651 427 245 166
Fragments arranged by size:
651 427 245 170 166
SmlI C'TyrA_G
Cuts at: 0 571 1659
Size: 571 1088
StyI C'CwwG_G
Cuts at: 0 218 263 1398 1659
Size: 218 45 1135 261
Fragments arranged by size:
1135 261 218 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 697 1659
Size: 697 962
TatI w'GTAC_w
Cuts at: 0 137 1659
Size: 137 1522
XmaI C'CCGG_G
Cuts at: 0 168 819 1246 1491 1659
Size: 168 651 427 245 168
Fragments arranged by size:
651 427 245 168 168
ZraI GAC'GTC
Cuts at: 0 944 1659
Size: 944 715
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AlfI AlwNI ApaI
AscI AvaI BanI BanII BbeI BglI BlpI Bme1580I
BmgBI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BssHII BssSI BstEII BstXI
BtgI BtgZI BtsI DraIII EaeI EagI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI FspI FspAI HaeII Hin4I
KasI KpnI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PfoI PpiI PpuMI
PspOMI PsrI PstI RsrII SacI SacII SfcI SfiI
SfoI SmaI SmlI StyI TaqII TatI XmaI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AleI AloI
ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI
Bpu10I BsaBI BsiWI BsmI BsmBI BsrDI BsrGI BstAPI
BstBI BstYI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EarI EcoRI EcoRV FalI HincII HindIII HpaI MfeI
MluI MmeI NdeI NheI NruI NsiI PacI PflMI
PmeI PmlI PshAI PsiI PspXI PvuI PvuII SalI
SanDI SapI SbfI ScaI SexAI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmnI