Restriction map for
pFN21AB9440
(Linear) (Six-base) MAPSORT of: KIBB9440.seq Check: 576 from: 1 to: 1980
KIBB9440.orf
With 182 enzymes: SgfI *
May 17, 2016 15:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1150 1708 1978 1980
Size: 1150 558 270 2
Fragments arranged by size:
1150 558 270 2
AfeI AGC'GCT
Cuts at: 0 364 912 1980
Size: 364 548 1068
Fragments arranged by size:
1068 548 364
AflII C'TTAA_G
Cuts at: 0 1662 1980
Size: 1662 318
AleI CACnn'nnGTG
Cuts at: 0 793 1980
Size: 793 1187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 337 1980
Size: 337 1643
AlwNI CAG_nnn'CTG
Cuts at: 0 1807 1980
Size: 1807 173
ApaLI G'TGCA_C
Cuts at: 0 274 888 1324 1980
Size: 274 614 436 656
Fragments arranged by size:
656 614 436 274
ApoI r'AATT_y
Cuts at: 0 267 685 1634 1980
Size: 267 418 949 346
Fragments arranged by size:
949 418 346 267
AvaI C'yCGr_G
Cuts at: 0 79 869 1980
Size: 79 790 1111
Fragments arranged by size:
1111 790 79
BanI G'GyrC_C
Cuts at: 0 92 920 1980
Size: 92 828 1060
Fragments arranged by size:
1060 828 92
BanII G_rGCy'C
Cuts at: 0 876 1980
Size: 876 1104
BbeI G_GCGC'C
Cuts at: 0 96 1980
Size: 96 1884
BbsI GAAGACnn'nnnn_
Cuts at: 0 1210 1980
Size: 1210 770
BclI T'GATC_A
Cuts at: 0 1317 1980
Size: 1317 663
BfrBI ATG'CAT
Cuts at: 0 1825 1980
Size: 1825 155
BglI GCCn_nnn'nGGC
Cuts at: 0 534 1980
Size: 534 1446
Bme1580I G_kGCm'C
Cuts at: 0 278 892 1328 1980
Size: 278 614 436 652
Fragments arranged by size:
652 614 436 278
BmrI ACTGGGnnnn_n'
Cuts at: 0 1370 1980
Size: 1370 610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1270 1980
Size: 1270 710
Bpu10I CC'TnA_GC
Cuts at: 0 290 1103 1980
Size: 290 813 877
Fragments arranged by size:
877 813 290
BsaI GGTCTCn'nnnn_
Cuts at: 0 1042 1980
Size: 1042 938
BsaAI yAC'GTr
Cuts at: 0 464 795 1980
Size: 464 331 1185
Fragments arranged by size:
1185 464 331
BsaHI Gr'CG_yC
Cuts at: 0 93 1980
Size: 93 1887
BsaWI w'CCGG_w
Cuts at: 0 1058 1980
Size: 1058 922
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1659 1689 1980
Size: 1659 30 291
Fragments arranged by size:
1659 291 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1618 1980
Size: 1618 362
BseYI C'CCAG_C
Cuts at: 0 572 977 1980
Size: 572 405 1003
Fragments arranged by size:
1003 572 405
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1490 1980
Size: 1490 490
BsiHKAI G_wGCw'C
Cuts at: 0 278 892 1328 1980
Size: 278 614 436 652
Fragments arranged by size:
652 614 436 278
Bsp1286I G_dGCh'C
Cuts at: 0 278 876 892 1328 1980
Size: 278 598 16 436 652
Fragments arranged by size:
652 598 436 278 16
BspHI T'CATG_A
Cuts at: 0 1277 1736 1980
Size: 1277 459 244
Fragments arranged by size:
1277 459 244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 20 1379 1389 1980
Size: 20 1359 10 591
Fragments arranged by size:
1359 591 20 10
BsrBI CCG'CTC
Cuts at: 0 24 1980
Size: 24 1956
BsrDI GCAATG_nn'
Cuts at: 0 893 1206 1587 1980
Size: 893 313 381 393
Fragments arranged by size:
893 393 381 313
BstYI r'GATC_y
Cuts at: 0 300 1980
Size: 300 1680
BtgI C'CryG_G
Cuts at: 0 830 1418 1980
Size: 830 588 562
Fragments arranged by size:
830 588 562
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 219 1980
Size: 219 1761
BtsI GCAGTG_nn'
Cuts at: 0 1007 1218 1963 1980
Size: 1007 211 745 17
Fragments arranged by size:
1007 745 211 17
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 944 979 1403 1438 1980
Size: 944 35 424 35 542
Fragments arranged by size:
944 542 424 35 35
DraI TTT'AAA
Cuts at: 0 264 1980
Size: 264 1716
DraIII CAC_nnn'GTG
Cuts at: 0 1599 1980
Size: 1599 381
EaeI y'GGCC_r
Cuts at: 0 1004 1411 1863 1980
Size: 1004 407 452 117
Fragments arranged by size:
1004 452 407 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 21 1980
Size: 21 1959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1150 1270 1708 1978 1980
Size: 1150 120 438 270 2
Fragments arranged by size:
1150 438 270 120 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 653 1980
Size: 653 1327
EcoO109I rG'GnC_Cy
Cuts at: 0 638 845 1086 1980
Size: 638 207 241 894
Fragments arranged by size:
894 638 241 207
EcoRV GAT'ATC
Cuts at: 0 1428 1980
Size: 1428 552
FspI TGC'GCA
Cuts at: 0 16 1980
Size: 16 1964
HaeII r_GCGC'y
Cuts at: 0 96 366 914 1980
Size: 96 270 548 1066
Fragments arranged by size:
1066 548 270 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 537 569 1659 1691 1731 1763 1980
Size: 537 32 1090 32 40 32 217
Fragments arranged by size:
1090 537 217 40 32 32 32
HincII GTy'rAC
Cuts at: 0 1563 1980
Size: 1563 417
KasI G'GCGC_C
Cuts at: 0 92 1980
Size: 92 1888
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 1980
Size: 1156 824
MscI TGG'CCA
Cuts at: 0 1006 1413 1980
Size: 1006 407 567
Fragments arranged by size:
1006 567 407
MslI CAynn'nnrTG
Cuts at: 0 208 757 793 1211 1276 1980
Size: 208 549 36 418 65 704
Fragments arranged by size:
704 549 418 208 65 36
MspA1I CmG'CkG
Cuts at: 0 804 1123 1980
Size: 804 319 857
Fragments arranged by size:
857 804 319
NarI GG'CG_CC
Cuts at: 0 93 1980
Size: 93 1887
NcoI C'CATG_G
Cuts at: 0 830 1418 1980
Size: 830 588 562
Fragments arranged by size:
830 588 562
NsiI A_TGCA'T
Cuts at: 0 1827 1980
Size: 1827 153
NspI r_CATG'y
Cuts at: 0 318 610 756 1175 1980
Size: 318 292 146 419 805
Fragments arranged by size:
805 419 318 292 146
PflMI CCAn_nnn'nTGG
Cuts at: 0 521 1029 1599 1980
Size: 521 508 570 381
Fragments arranged by size:
570 521 508 381
PfoI T'CCnGG_A
Cuts at: 0 65 1144 1980
Size: 65 1079 836
Fragments arranged by size:
1079 836 65
PpuMI rG'GwC_Cy
Cuts at: 0 638 845 1086 1980
Size: 638 207 241 894
Fragments arranged by size:
894 638 241 207
PstI C_TGCA'G
Cuts at: 0 1970 1980
Size: 1970 10
SanDI GG'GwC_CC
Cuts at: 0 638 1980
Size: 638 1342
SfcI C'TryA_G
Cuts at: 0 1966 1980
Size: 1966 14
SfoI GGC'GCC
Cuts at: 0 94 1980
Size: 94 1886
SmlI C'TyrA_G
Cuts at: 0 1662 1980
Size: 1662 318
SspI AAT'ATT
Cuts at: 0 1227 1980
Size: 1227 753
StyI C'CwwG_G
Cuts at: 0 830 1418 1980
Size: 830 588 562
Fragments arranged by size:
830 588 562
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1170 1202 1325 1357 1980
Size: 1170 32 123 32 623
Fragments arranged by size:
1170 623 123 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 546 1135 1517 1980
Size: 546 589 382 463
Fragments arranged by size:
589 546 463 382
Enzymes that do cut:
AcuI AfeI AflII AleI AlfI AlwNI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BclI BfrBI BglI
Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII EaeI EciI Eco57MI EcoNI EcoO109I EcoRV FspI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NarI NcoI NsiI NspI PflMI PfoI PpuMI PstI
SanDI SfcI SfoI SmlI SspI StyI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BglII BlpI BmgBI BmtI BplI
BpuEI BsaBI BsiEI BsiWI BsmI BsmBI BspEI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I ClaI DrdI EagI EarI EcoICRI EcoRI FalI
FseI FspAI HindIII HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmaI ZraI