(Linear) (Six-base) MAPSORT of: KIBB9438.seq  Check: 3908  from: 1  to: 549



KIBB9438.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:32  ..

AccI GT'mk_AC

Cuts at:      0     446     549 
   Size:        446     103
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     334     406     549 
   Size:        133     201      72     143

  Fragments arranged by size: 

                 201     143     133      72
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     158     549 
   Size:        158     391
 
AlwNI CAG_nnn'CTG

Cuts at:      0      99     549 
   Size:         99     450
 
ApoI r'AATT_y

Cuts at:      0     173     535     549 
   Size:        173     362      14

  Fragments arranged by size: 

                 362     173      14
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     148     181     549 
   Size:        148      33     368

  Fragments arranged by size: 

                 368     148      33
 
BanII G_rGCy'C

Cuts at:      0     374     549 
   Size:        374     175
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     176     549 
   Size:        176     373
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     109     549 
   Size:        109     440
 
BsaBI GATnn'nnATC

Cuts at:      0     514     549 
   Size:        514      35
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     457     487     549 
   Size:        457      30      62

  Fragments arranged by size: 

                 457      62      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     382     549 
   Size:        382     167
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     283     549 
   Size:        118     165     266

  Fragments arranged by size: 

                 266     165     118
 
Bsp1286I G_dGCh'C

Cuts at:      0     374     549 
   Size:        374     175
 
BspHI T'CATG_A

Cuts at:      0     254     549 
   Size:        254     295
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     496     549 
   Size:        496      53
 
EarI CTCTTCn'nnn_

Cuts at:      0      17     152     382     549 
   Size:         17     135     230     167

  Fragments arranged by size: 

                 230     167     135      17
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     515     549 
   Size:        515      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     334     406     549 
   Size:        133     201      72     143

  Fragments arranged by size: 

                 201     143     133      72
 
EcoRI G'AATT_C

Cuts at:      0     173     535     549 
   Size:        173     362      14

  Fragments arranged by size: 

                 362     173      14
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     339     549 
   Size:        339     210
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     457     489     549 
   Size:        457      32      60

  Fragments arranged by size: 

                 457      60      32
 
HincII GTy'rAC

Cuts at:      0     339     405     549 
   Size:        339      66     144

  Fragments arranged by size: 

                 339     144      66
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      36     405     549 
   Size:         36     369     144

  Fragments arranged by size: 

                 369     144      36
 
MslI CAynn'nnrTG

Cuts at:      0     259     494     549 
   Size:        259     235      55

  Fragments arranged by size: 

                 259     235      55
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      18     549 
   Size:         18     531
 
SapI GCTCTTCn'nnn_

Cuts at:      0     152     549 
   Size:        152     397
 
TatI w'GTAC_w

Cuts at:      0     189     549 
   Size:        189     360
 
Tth111I GACn'n_nGTC

Cuts at:      0     442     549 
   Size:        442     107
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     526     549 
   Size:        526      23
 
XmnI GAAnn'nnTTC

Cuts at:      0     220     509     549 
   Size:        220     289      40

  Fragments arranged by size: 

                 289     220      40
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI     ApoI     BaeI    BanII    BciVI
     BmrI    BsaBI    BsaXI    BseRI     BsgI Bsp1286I    BspHI    BstXI
     EarI     EciI  Eco57MI    EcoRI     FalI    Hin4I   HincII     MmeI
     MslI    PflMI     SapI     TatI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI     XbaI     XhoI     XmaI     ZraI