Restriction map for
pFN21AB9139
(Linear) (Six-base) MAPSORT of: KIBB9139.seq Check: 8035 from: 1 to: 1461
KIBB9139.orf
With 182 enzymes: SgfI *
November 15, 2013 14:41 ..
AccI GT'mk_AC
Cuts at: 0 152 401 1461
Size: 152 249 1060
Fragments arranged by size:
1060 249 152
Acc65I G'GTAC_C
Cuts at: 0 611 1461
Size: 611 850
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 696 829 1021 1461
Size: 637 59 133 192 440
Fragments arranged by size:
637 440 192 133 59
AflIII A'CryG_T
Cuts at: 0 989 1461
Size: 989 472
AhdI GACnn_n'nnGTC
Cuts at: 0 93 1461
Size: 93 1368
ApaI G_GGCC'C
Cuts at: 0 1138 1461
Size: 1138 323
AvaI C'yCGr_G
Cuts at: 0 40 1461
Size: 40 1421
BanI G'GyrC_C
Cuts at: 0 51 163 611 1461
Size: 51 112 448 850
Fragments arranged by size:
850 448 112 51
BanII G_rGCy'C
Cuts at: 0 189 1138 1461
Size: 189 949 323
Fragments arranged by size:
949 323 189
BbsI GAAGACnn'nnnn_
Cuts at: 0 625 793 817 1047 1415 1461
Size: 625 168 24 230 368 46
Fragments arranged by size:
625 368 230 168 46 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1353 1387 1461
Size: 1353 34 74
Fragments arranged by size:
1353 74 34
BclI T'GATC_A
Cuts at: 0 653 1461
Size: 653 808
BglI GCCn_nnn'nGGC
Cuts at: 0 90 1165 1461
Size: 90 1075 296
Fragments arranged by size:
1075 296 90
BglII A'GATC_T
Cuts at: 0 1091 1461
Size: 1091 370
Bme1580I G_kGCm'C
Cuts at: 0 56 1138 1461
Size: 56 1082 323
Fragments arranged by size:
1082 323 56
BmgBI CAC'GTC
Cuts at: 0 747 1461
Size: 747 714
BmrI ACTGGGnnnn_n'
Cuts at: 0 1233 1405 1461
Size: 1233 172 56
Fragments arranged by size:
1233 172 56
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 265 393 700 712 909 1461
Size: 94 171 128 307 12 197 552
Fragments arranged by size:
552 307 197 171 128 94 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1461
Size: 814 647
BsaI GGTCTCn'nnnn_
Cuts at: 0 1318 1461
Size: 1318 143
BsaHI Gr'CG_yC
Cuts at: 0 23 1461
Size: 23 1438
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1315 1345 1461
Size: 1315 30 116
Fragments arranged by size:
1315 116 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 1461
Size: 91 1370
BseYI C'CCAG_C
Cuts at: 0 480 765 896 1131 1416 1461
Size: 480 285 131 235 285 45
Fragments arranged by size:
480 285 285 235 131 45
BsiEI CG_ry'CG
Cuts at: 0 450 1461
Size: 450 1011
BsiHKAI G_wGCw'C
Cuts at: 0 206 698 752 1031 1449 1461
Size: 206 492 54 279 418 12
Fragments arranged by size:
492 418 279 206 54 12
Bsp1286I G_dGCh'C
Cuts at: 0 56 189 206 698 752 1031 1138 1449
Size: 56 133 17 492 54 279 107 311
Cuts at: 1449 1461
Size: 12
Fragments arranged by size:
492 311 279 133 107 56 54 17
12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1421 1461
Size: 1421 40
BsrFI r'CCGG_y
Cuts at: 0 100 449 918 1461
Size: 100 349 469 543
Fragments arranged by size:
543 469 349 100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 702 776 1461
Size: 702 74 685
Fragments arranged by size:
702 685 74
BstXI CCAn_nnnn'nTGG
Cuts at: 0 303 371 1461
Size: 303 68 1090
Fragments arranged by size:
1090 303 68
BstYI r'GATC_y
Cuts at: 0 1091 1461
Size: 1091 370
Bsu36I CC'TnA_GG
Cuts at: 0 257 1461
Size: 257 1204
BtgI C'CryG_G
Cuts at: 0 34 56 1169 1461
Size: 34 22 1113 292
Fragments arranged by size:
1113 292 34 22
BtsI GCAGTG_nn'
Cuts at: 0 490 1461
Size: 490 971
DrdI GACnn_nn'nnGTC
Cuts at: 0 751 1461
Size: 751 710
EaeI y'GGCC_r
Cuts at: 0 102 293 447 944 1079 1157 1166 1430
Size: 102 191 154 497 135 78 9 264
Cuts at: 1430 1461
Size: 31
Fragments arranged by size:
497 264 191 154 135 102 78 31
9
EagI C'GGCC_G
Cuts at: 0 447 1461
Size: 447 1014
EarI CTCTTCn'nnn_
Cuts at: 0 211 433 859 1461
Size: 211 222 426 602
Fragments arranged by size:
602 426 222 211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 265 393 637 696 700 712 829
Size: 94 171 128 244 59 4 12 117
Cuts at: 829 909 1021 1461
Size: 80 112 440
Fragments arranged by size:
440 244 171 128 117 112 94 80
59 12 4
EcoO109I rG'GnC_Cy
Cuts at: 0 65 828 1420 1461
Size: 65 763 592 41
Fragments arranged by size:
763 592 65 41
HaeII r_GCGC'y
Cuts at: 0 1270 1290 1461
Size: 1270 20 171
Fragments arranged by size:
1270 171 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 787 819 1461
Size: 79 32 676 32 642
Fragments arranged by size:
676 642 79 32 32
HincII GTy'rAC
Cuts at: 0 471 1461
Size: 471 990
KpnI G_GTAC'C
Cuts at: 0 615 1461
Size: 615 846
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 868 1461
Size: 557 311 593
Fragments arranged by size:
593 557 311
MscI TGG'CCA
Cuts at: 0 295 1081 1461
Size: 295 786 380
Fragments arranged by size:
786 380 295
MslI CAynn'nnrTG
Cuts at: 0 661 745 964 1186 1461
Size: 661 84 219 222 275
Fragments arranged by size:
661 275 222 219 84
MspA1I CmG'CkG
Cuts at: 0 36 58 702 896 1461
Size: 36 22 644 194 565
Fragments arranged by size:
644 565 194 36 22
NaeI GCC'GGC
Cuts at: 0 102 451 920 1461
Size: 102 349 469 541
Fragments arranged by size:
541 469 349 102
NgoMIV G'CCGG_C
Cuts at: 0 100 449 918 1461
Size: 100 349 469 543
Fragments arranged by size:
543 469 349 100
PfoI T'CCnGG_A
Cuts at: 0 440 677 1461
Size: 440 237 784
Fragments arranged by size:
784 440 237
PspOMI G'GGCC_C
Cuts at: 0 1134 1461
Size: 1134 327
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 979 1011 1461
Size: 979 32 450
Fragments arranged by size:
979 450 32
PvuII CAG'CTG
Cuts at: 0 702 896 1461
Size: 702 194 565
Fragments arranged by size:
702 565 194
SacII CC_GC'GG
Cuts at: 0 37 59 1461
Size: 37 22 1402
Fragments arranged by size:
1402 37 22
SapI GCTCTTCn'nnn_
Cuts at: 0 211 1461
Size: 211 1250
SexAI A'CCwGG_T
Cuts at: 0 1034 1461
Size: 1034 427
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1165 1461
Size: 1165 296
SmlI C'TyrA_G
Cuts at: 0 793 1461
Size: 793 668
StuI AGG'CCT
Cuts at: 0 1144 1461
Size: 1144 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1060 1406 1461
Size: 1060 346 55
Fragments arranged by size:
1060 346 55
TatI w'GTAC_w
Cuts at: 0 683 1461
Size: 683 778
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1315 1347 1461
Size: 1315 32 114
Fragments arranged by size:
1315 114 32
XmnI GAAnn'nnTTC
Cuts at: 0 991 1461
Size: 991 470
Enzymes that do cut:
AccI Acc65I AcuI AflIII AhdI ApaI AvaI BanI
BanII BbsI BcgI BclI BglI BglII Bme1580I BmgBI
BmrI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstXI BstYI
Bsu36I BtgI BtsI DrdI EaeI EagI EarI Eco57MI
EcoO109I HaeII Hin4I HincII KpnI MmeI MscI MslI
MspA1I NaeI NgoMIV PfoI PspOMI PsrI PvuII SacII
SapI SexAI SfiI SmlI StuI TaqII TatI TstI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BfrBI BlpI BmtI
BplI Bpu10I BsaAI BsaBI BsaWI BsgI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI MfeI MluI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI RsrII SacI SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StyI SwaI Tth111I XbaI XcmI
XhoI XmaI ZraI