Restriction map for
pFN21AB9099
(Linear) (Six-base) MAPSORT of: KIBB9099.seq Check: 5081 from: 1 to: 666
KIBB9099.orf
With 182 enzymes: SgfI *
May 29, 2012 11:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 231 321 666
Size: 40 191 90 345
Fragments arranged by size:
345 191 90 40
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 462 666
Size: 462 204
AlwNI CAG_nnn'CTG
Cuts at: 0 368 666
Size: 368 298
ApaI G_GGCC'C
Cuts at: 0 212 666
Size: 212 454
ApoI r'AATT_y
Cuts at: 0 286 666
Size: 286 380
AvaI C'yCGr_G
Cuts at: 0 223 385 658 666
Size: 223 162 273 8
Fragments arranged by size:
273 223 162 8
BanII G_rGCy'C
Cuts at: 0 109 212 274 659 666
Size: 109 103 62 385 7
Fragments arranged by size:
385 109 103 62 7
BglII A'GATC_T
Cuts at: 0 278 666
Size: 278 388
BlpI GC'TnA_GC
Cuts at: 0 103 653 666
Size: 103 550 13
Fragments arranged by size:
550 103 13
Bme1580I G_kGCm'C
Cuts at: 0 212 666
Size: 212 454
BmrI ACTGGGnnnn_n'
Cuts at: 0 102 666
Size: 102 564
BsaI GGTCTCn'nnnn_
Cuts at: 0 448 592 666
Size: 448 144 74
Fragments arranged by size:
448 144 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 517 547 666
Size: 517 30 119
Fragments arranged by size:
517 119 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 562 666
Size: 562 104
BsiHKAI G_wGCw'C
Cuts at: 0 122 274 665 666
Size: 122 152 391 1
Fragments arranged by size:
391 152 122 1
BsmI GAATG_Cn'
Cuts at: 0 623 666
Size: 623 43
BsmBI CGTCTCn'nnnn_
Cuts at: 0 462 666
Size: 462 204
Bsp1286I G_dGCh'C
Cuts at: 0 109 122 212 274 659 665 666
Size: 109 13 90 62 385 6 1
Fragments arranged by size:
385 109 90 62 13 6 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 142 211 430 666
Size: 142 69 219 236
Fragments arranged by size:
236 219 142 69
BstAPI GCAn_nnn'nTGC
Cuts at: 0 203 666
Size: 203 463
BstBI TT'CG_AA
Cuts at: 0 284 666
Size: 284 382
BstEII G'GTnAC_C
Cuts at: 0 81 666
Size: 81 585
BstYI r'GATC_y
Cuts at: 0 278 666
Size: 278 388
BtgI C'CryG_G
Cuts at: 0 77 185 631 666
Size: 77 108 446 35
Fragments arranged by size:
446 108 77 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 312 666
Size: 312 354
EaeI y'GGCC_r
Cuts at: 0 65 666
Size: 65 601
EarI CTCTTCn'nnn_
Cuts at: 0 43 388 666
Size: 43 345 278
Fragments arranged by size:
345 278 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 177 666
Size: 177 489
EcoICRI GAG'CTC
Cuts at: 0 272 666
Size: 272 394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 231 321 666
Size: 40 191 90 345
Fragments arranged by size:
345 191 90 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 246 666
Size: 246 420
EcoRI G'AATT_C
Cuts at: 0 286 666
Size: 286 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 387 419 666
Size: 387 32 247
Fragments arranged by size:
387 247 32
MscI TGG'CCA
Cuts at: 0 67 666
Size: 67 599
MslI CAynn'nnrTG
Cuts at: 0 184 666
Size: 184 482
NcoI C'CATG_G
Cuts at: 0 185 631 666
Size: 185 446 35
Fragments arranged by size:
446 185 35
PshAI GACnn'nnGTC
Cuts at: 0 417 666
Size: 417 249
PspOMI G'GGCC_C
Cuts at: 0 208 666
Size: 208 458
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 226 258 666
Size: 226 32 408
Fragments arranged by size:
408 226 32
SacI G_AGCT'C
Cuts at: 0 274 666
Size: 274 392
ScaI AGT'ACT
Cuts at: 0 526 666
Size: 526 140
SmaI CCC'GGG
Cuts at: 0 225 666
Size: 225 441
StyI C'CwwG_G
Cuts at: 0 185 342 631 666
Size: 185 157 289 35
Fragments arranged by size:
289 185 157 35
TatI w'GTAC_w
Cuts at: 0 305 524 666
Size: 305 219 142
Fragments arranged by size:
305 219 142
Tth111I GACn'n_nGTC
Cuts at: 0 511 666
Size: 511 155
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 75 666
Size: 75 591
XmaI C'CCGG_G
Cuts at: 0 223 666
Size: 223 443
Enzymes that do cut:
AcuI AlfI AlwNI ApaI ApoI AvaI BanII BglII
BlpI Bme1580I BmrI BsaI BsaXI BseRI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BstAPI BstBI BstEII BstYI BtgI
BtgZI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
Hin4I MscI MslI NcoI PshAI PspOMI PsrI SacI
ScaI SmaI StyI TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BmgBI BmtI BplI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiWI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstXI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII DrdI EagI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI
PstI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI XbaI XhoI XmnI ZraI