Restriction map for
pFN21AB9001
(Linear) (Six-base) MAPSORT of: KIBB9001.seq Check: 7804 from: 1 to: 1278
KIBB9001.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1118 1278
Size: 1118 160
AfeI AGC'GCT
Cuts at: 0 73 1278
Size: 73 1205
AflIII A'CryG_T
Cuts at: 0 562 1278
Size: 562 716
AhdI GACnn_n'nnGTC
Cuts at: 0 891 1278
Size: 891 387
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 638 670 717 749 1278
Size: 638 32 47 32 529
Fragments arranged by size:
638 529 47 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1218 1278
Size: 1218 60
ApoI r'AATT_y
Cuts at: 0 739 1083 1278
Size: 739 344 195
Fragments arranged by size:
739 344 195
BanI G'GyrC_C
Cuts at: 0 993 1278
Size: 993 285
BanII G_rGCy'C
Cuts at: 0 108 579 1278
Size: 108 471 699
Fragments arranged by size:
699 471 108
BbeI G_GCGC'C
Cuts at: 0 997 1278
Size: 997 281
BbsI GAAGACnn'nnnn_
Cuts at: 0 264 1278
Size: 264 1014
BfrBI ATG'CAT
Cuts at: 0 638 1278
Size: 638 640
BlpI GC'TnA_GC
Cuts at: 0 169 1278
Size: 169 1109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1184 1278
Size: 1184 94
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1278
Size: 313 965
Bpu10I CC'TnA_GC
Cuts at: 0 107 1278
Size: 107 1171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 1278
Size: 52 1226
BsaHI Gr'CG_yC
Cuts at: 0 941 994 1278
Size: 941 53 284
Fragments arranged by size:
941 284 53
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 638 668 1180 1210 1278
Size: 638 30 512 30 68
Fragments arranged by size:
638 512 68 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 310 1185 1278
Size: 310 875 93
Fragments arranged by size:
875 310 93
BseYI C'CCAG_C
Cuts at: 0 840 915 978 1278
Size: 840 75 63 300
Fragments arranged by size:
840 300 75 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1160 1278
Size: 1160 118
BsiHKAI G_wGCw'C
Cuts at: 0 857 1278
Size: 857 421
Bsp1286I G_dGCh'C
Cuts at: 0 108 579 857 1278
Size: 108 471 278 421
Fragments arranged by size:
471 421 278 108
BspHI T'CATG_A
Cuts at: 0 227 539 743 1278
Size: 227 312 204 535
Fragments arranged by size:
535 312 227 204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 398 1278
Size: 398 880
BstXI CCAn_nnnn'nTGG
Cuts at: 0 433 1278
Size: 433 845
Bsu36I CC'TnA_GG
Cuts at: 0 581 1278
Size: 581 697
BtsI GCAGTG_nn'
Cuts at: 0 1151 1161 1278
Size: 1151 10 117
Fragments arranged by size:
1151 117 10
DraI TTT'AAA
Cuts at: 0 455 1278
Size: 455 823
EaeI y'GGCC_r
Cuts at: 0 155 515 1278
Size: 155 360 763
Fragments arranged by size:
763 360 155
EarI CTCTTCn'nnn_
Cuts at: 0 826 841 1278
Size: 826 15 437
Fragments arranged by size:
826 437 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1118 1278
Size: 313 805 160
Fragments arranged by size:
805 313 160
EcoO109I rG'GnC_Cy
Cuts at: 0 1258 1278
Size: 1258 20
EcoRI G'AATT_C
Cuts at: 0 739 1083 1278
Size: 739 344 195
Fragments arranged by size:
739 344 195
HaeII r_GCGC'y
Cuts at: 0 75 997 1278
Size: 75 922 281
Fragments arranged by size:
922 281 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 367 399 610 642 877 909 1180 1212
Size: 367 32 211 32 235 32 271 32
Cuts at: 1212 1278
Size: 66
Fragments arranged by size:
367 271 235 211 66 32 32 32
32
HincII GTy'rAC
Cuts at: 0 18 1278
Size: 18 1260
HindIII A'AGCT_T
Cuts at: 0 534 1278
Size: 534 744
KasI G'GCGC_C
Cuts at: 0 993 1278
Size: 993 285
MfeI C'AATT_G
Cuts at: 0 1043 1278
Size: 1043 235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 1278
Size: 1004 274
MscI TGG'CCA
Cuts at: 0 157 517 1278
Size: 157 360 761
Fragments arranged by size:
761 360 157
MspA1I CmG'CkG
Cuts at: 0 844 1278
Size: 844 434
NarI GG'CG_CC
Cuts at: 0 994 1278
Size: 994 284
NdeI CA'TA_TG
Cuts at: 0 552 1278
Size: 552 726
NsiI A_TGCA'T
Cuts at: 0 640 1278
Size: 640 638
NspI r_CATG'y
Cuts at: 0 199 1278
Size: 199 1079
PasI CC'CwG_GG
Cuts at: 0 1263 1278
Size: 1263 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 1218 1278
Size: 1218 60
PfoI T'CCnGG_A
Cuts at: 0 500 675 1278
Size: 500 175 603
Fragments arranged by size:
603 500 175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 638 670 1278
Size: 638 32 608
Fragments arranged by size:
638 608 32
PpuMI rG'GwC_Cy
Cuts at: 0 1258 1278
Size: 1258 20
SfcI C'TryA_G
Cuts at: 0 30 1278
Size: 30 1248
SfoI GGC'GCC
Cuts at: 0 995 1278
Size: 995 283
SmlI C'TyrA_G
Cuts at: 0 67 1278
Size: 67 1211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 406 1278
Size: 406 872
XmnI GAAnn'nnTTC
Cuts at: 0 653 1278
Size: 653 625
Enzymes that do cut:
AcuI AfeI AflIII AhdI AloI AlwNI ApoI BanI
BanII BbeI BbsI BfrBI BlpI BplI BpmI Bpu10I
BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BspMI BstXI Bsu36I BtsI DraI EaeI EarI
Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HindIII KasI
MfeI MmeI MscI MspA1I NarI NdeI NsiI NspI
PasI PflMI PfoI PpiI PpuMI SfcI SfoI SmlI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BglI BglII
Bme1580I BmgBI BmrI BmtI BsaI BsaAI BsaBI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI ClaI CspCI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HpaI
KpnI MluI MslI NaeI NcoI NgoMIV NheI NotI
NruI PacI PciI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI ZraI