Restriction map for
pFN21AB8975
(Linear) (Six-base) MAPSORT of: KIBB8975.seq Check: 9485 from: 1 to: 1113
KIBB8975.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 504 1113
Size: 504 609
AflIII A'CryG_T
Cuts at: 0 768 1113
Size: 768 345
AlwNI CAG_nnn'CTG
Cuts at: 0 40 684 1113
Size: 40 644 429
Fragments arranged by size:
644 429 40
ApaI G_GGCC'C
Cuts at: 0 391 1113
Size: 391 722
AvaI C'yCGr_G
Cuts at: 0 100 948 1056 1113
Size: 100 848 108 57
Fragments arranged by size:
848 108 100 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 535 568 1113
Size: 535 33 545
Fragments arranged by size:
545 535 33
BanI G'GyrC_C
Cuts at: 0 138 176 184 222 343 561 721 728
Size: 138 38 8 38 121 218 160 7
Cuts at: 728 777 817 832 928 1072 1113
Size: 49 40 15 96 144 41
Fragments arranged by size:
218 160 144 138 121 96 49 41
40 38 38 15 8 7
BanII G_rGCy'C
Cuts at: 0 108 391 433 1063 1113
Size: 108 283 42 630 50
Fragments arranged by size:
630 283 108 50 42
BbeI G_GCGC'C
Cuts at: 0 188 347 565 836 932 1113
Size: 188 159 218 271 96 181
Fragments arranged by size:
271 218 188 181 159 96
BciVI GTATCCnnnnn_n'
Cuts at: 0 1041 1113
Size: 1041 72
BglI GCCn_nnn'nGGC
Cuts at: 0 848 892 1113
Size: 848 44 221
Fragments arranged by size:
848 221 44
BglII A'GATC_T
Cuts at: 0 617 1113
Size: 617 496
BlpI GC'TnA_GC
Cuts at: 0 749 1113
Size: 749 364
Bme1580I G_kGCm'C
Cuts at: 0 179 227 391 724 733 822 1113
Size: 179 48 164 333 9 89 291
Fragments arranged by size:
333 291 179 164 89 48 9
BmgBI CAC'GTC
Cuts at: 0 444 1113
Size: 444 669
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 547 1113
Size: 79 468 566
Fragments arranged by size:
566 468 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 1113
Size: 69 1044
BsaHI Gr'CG_yC
Cuts at: 0 185 344 562 601 833 929 1113
Size: 185 159 218 39 232 96 184
Fragments arranged by size:
232 218 185 184 159 96 39
BsaWI w'CCGG_w
Cuts at: 0 981 1113
Size: 981 132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1036 1066 1113
Size: 1036 30 47
Fragments arranged by size:
1036 47 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1044 1113
Size: 1044 69
BseYI C'CCAG_C
Cuts at: 0 227 435 1113
Size: 227 208 678
Fragments arranged by size:
678 227 208
BsiEI CG_ry'CG
Cuts at: 0 1104 1113
Size: 1104 9
BsiHKAI G_wGCw'C
Cuts at: 0 500 578 1113
Size: 500 78 535
Fragments arranged by size:
535 500 78
Bsp1286I G_dGCh'C
Cuts at: 0 108 179 227 391 433 500 578 724
Size: 108 71 48 164 42 67 78 146
Cuts at: 724 733 822 1063 1113
Size: 9 89 241 50
Fragments arranged by size:
241 164 146 108 89 78 71 67
50 48 42 9
BspEI T'CCGG_A
Cuts at: 0 981 1113
Size: 981 132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 504 600 1113
Size: 504 96 513
Fragments arranged by size:
513 504 96
BsrBI CCG'CTC
Cuts at: 0 398 413 1113
Size: 398 15 700
Fragments arranged by size:
700 398 15
BsrFI r'CCGG_y
Cuts at: 0 780 931 1113
Size: 780 151 182
Fragments arranged by size:
780 182 151
BssHII G'CGCG_C
Cuts at: 0 473 1113
Size: 473 640
BstAPI GCAn_nnn'nTGC
Cuts at: 0 684 728 806 1113
Size: 684 44 78 307
Fragments arranged by size:
684 307 78 44
BstXI CCAn_nnnn'nTGG
Cuts at: 0 915 1113
Size: 915 198
BstYI r'GATC_y
Cuts at: 0 617 1113
Size: 617 496
BtgI C'CryG_G
Cuts at: 0 170 180 245 914 1010 1113
Size: 170 10 65 669 96 103
Fragments arranged by size:
669 170 103 96 65 10
DraIII CAC_nnn'GTG
Cuts at: 0 171 728 1113
Size: 171 557 385
Fragments arranged by size:
557 385 171
EaeI y'GGCC_r
Cuts at: 0 152 581 905 1113
Size: 152 429 324 208
Fragments arranged by size:
429 324 208 152
EarI CTCTTCn'nnn_
Cuts at: 0 505 1113
Size: 505 608
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 231 1113
Size: 231 882
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 547 1113
Size: 79 468 566
Fragments arranged by size:
566 468 79
HaeII r_GCGC'y
Cuts at: 0 188 323 347 492 565 654 836 932
Size: 188 135 24 145 73 89 182 96
Cuts at: 932 1018 1113
Size: 86 95
Fragments arranged by size:
188 182 145 135 96 95 89 86
73 24
KasI G'GCGC_C
Cuts at: 0 184 343 561 832 928 1113
Size: 184 159 218 271 96 185
Fragments arranged by size:
271 218 185 184 159 96
MluI A'CGCG_T
Cuts at: 0 768 1113
Size: 768 345
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1084 1113
Size: 1084 29
MscI TGG'CCA
Cuts at: 0 583 1113
Size: 583 530
MslI CAynn'nnrTG
Cuts at: 0 406 913 1113
Size: 406 507 200
Fragments arranged by size:
507 406 200
MspA1I CmG'CkG
Cuts at: 0 111 151 465 698 904 923 1012 1113
Size: 111 40 314 233 206 19 89 101
Fragments arranged by size:
314 233 206 111 101 89 40 19
NaeI GCC'GGC
Cuts at: 0 933 1113
Size: 933 180
NarI GG'CG_CC
Cuts at: 0 185 344 562 833 929 1113
Size: 185 159 218 271 96 184
Fragments arranged by size:
271 218 185 184 159 96
NgoMIV G'CCGG_C
Cuts at: 0 931 1113
Size: 931 182
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 307 339 1113
Size: 307 32 774
Fragments arranged by size:
774 307 32
PspOMI G'GGCC_C
Cuts at: 0 387 1113
Size: 387 726
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 622 654 1113
Size: 622 32 459
Fragments arranged by size:
622 459 32
PstI C_TGCA'G
Cuts at: 0 689 1022 1113
Size: 689 333 91
Fragments arranged by size:
689 333 91
SacII CC_GC'GG
Cuts at: 0 1013 1113
Size: 1013 100
SapI GCTCTTCn'nnn_
Cuts at: 0 505 1113
Size: 505 608
SfcI C'TryA_G
Cuts at: 0 685 1018 1113
Size: 685 333 95
Fragments arranged by size:
685 333 95
SfoI GGC'GCC
Cuts at: 0 186 345 563 834 930 1113
Size: 186 159 218 271 96 183
Fragments arranged by size:
271 218 186 183 159 96
SmaI CCC'GGG
Cuts at: 0 950 1113
Size: 950 163
SmlI C'TyrA_G
Cuts at: 0 48 1113
Size: 48 1065
StyI C'CwwG_G
Cuts at: 0 659 936 1113
Size: 659 277 177
Fragments arranged by size:
659 277 177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1036 1068 1113
Size: 1036 32 45
Fragments arranged by size:
1036 45 32
XmaI C'CCGG_G
Cuts at: 0 948 1113
Size: 948 165
Enzymes that do cut:
AarI AflIII AlwNI ApaI AvaI BaeI BanI BanII
BbeI BciVI BglI BglII BlpI Bme1580I BmgBI BpmI
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstAPI BstXI
BstYI BtgI DraIII EaeI EarI EciI Eco57MI HaeII
KasI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV PpiI PspOMI PsrI PstI SacII SapI SfcI
SfoI SmaI SmlI StyI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BamHI BbsI BbvCI BcgI BclI BfrBI
BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsgI
BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI
PshAI PsiI PspXI PvuI PvuII RsrII SacI SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmnI ZraI