Restriction map for
pFN21AB8971
(Linear) (Six-base) MAPSORT of: KIBB8971.seq Check: 1609 from: 1 to: 1086
KIBB8971.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 248 343 621 1086
Size: 248 95 278 465
Fragments arranged by size:
465 278 248 95
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 292 519 1086
Size: 99 193 227 567
Fragments arranged by size:
567 227 193 99
AfeI AGC'GCT
Cuts at: 0 434 1086
Size: 434 652
AhdI GACnn_n'nnGTC
Cuts at: 0 1067 1086
Size: 1067 19
AlwNI CAG_nnn'CTG
Cuts at: 0 960 1086
Size: 960 126
ApaI G_GGCC'C
Cuts at: 0 719 1086
Size: 719 367
AvaI C'yCGr_G
Cuts at: 0 586 718 1086
Size: 586 132 368
Fragments arranged by size:
586 368 132
BanII G_rGCy'C
Cuts at: 0 306 719 1086
Size: 306 413 367
Fragments arranged by size:
413 367 306
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 203 237 849 883 1086
Size: 203 34 612 34 203
Fragments arranged by size:
612 203 203 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 801 1086
Size: 801 285
BlpI GC'TnA_GC
Cuts at: 0 71 1047 1086
Size: 71 976 39
Fragments arranged by size:
976 71 39
Bme1580I G_kGCm'C
Cuts at: 0 719 1086
Size: 719 367
BmrI ACTGGGnnnn_n'
Cuts at: 0 128 1086
Size: 128 958
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 336 1086
Size: 229 107 750
Fragments arranged by size:
750 229 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 486 1054 1086
Size: 486 568 32
Fragments arranged by size:
568 486 32
BsaAI yAC'GTr
Cuts at: 0 417 1086
Size: 417 669
BsaHI Gr'CG_yC
Cuts at: 0 662 1086
Size: 662 424
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 349 379 466 496 1057 1086
Size: 349 30 87 30 561 29
Fragments arranged by size:
561 349 87 30 30 29
BseYI C'CCAG_C
Cuts at: 0 159 438 657 1086
Size: 159 279 219 429
Fragments arranged by size:
429 279 219 159
BsiEI CG_ry'CG
Cuts at: 0 411 468 1086
Size: 411 57 618
Fragments arranged by size:
618 411 57
Bsp1286I G_dGCh'C
Cuts at: 0 306 719 1086
Size: 306 413 367
Fragments arranged by size:
413 367 306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 12 248 343 621 968 1086
Size: 12 236 95 278 347 118
Fragments arranged by size:
347 278 236 118 95 12
BsrBI CCG'CTC
Cuts at: 0 837 1086
Size: 837 249
BsrDI GCAATG_nn'
Cuts at: 0 180 1086
Size: 180 906
BsrFI r'CCGG_y
Cuts at: 0 169 376 725 1025 1086
Size: 169 207 349 300 61
Fragments arranged by size:
349 300 207 169 61
BssHII G'CGCG_C
Cuts at: 0 217 829 1086
Size: 217 612 257
Fragments arranged by size:
612 257 217
BstEII G'GTnAC_C
Cuts at: 0 879 1086
Size: 879 207
BstXI CCAn_nnnn'nTGG
Cuts at: 0 43 198 891 1086
Size: 43 155 693 195
Fragments arranged by size:
693 195 155 43
Bsu36I CC'TnA_GG
Cuts at: 0 993 1086
Size: 993 93
BtgI C'CryG_G
Cuts at: 0 149 448 623 1011 1055 1086
Size: 149 299 175 388 44 31
Fragments arranged by size:
388 299 175 149 44 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 408 1086
Size: 408 678
BtsI GCAGTG_nn'
Cuts at: 0 76 505 871 1086
Size: 76 429 366 215
Fragments arranged by size:
429 366 215 76
EaeI y'GGCC_r
Cuts at: 0 167 188 257 419 465 704 1086
Size: 167 21 69 162 46 239 382
Fragments arranged by size:
382 239 167 162 69 46 21
EagI C'GGCC_G
Cuts at: 0 465 1086
Size: 465 621
EarI CTCTTCn'nnn_
Cuts at: 0 47 311 391 538 1086
Size: 47 264 80 147 548
Fragments arranged by size:
548 264 147 80 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 940 1086
Size: 940 146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 229 292 336 519 1086
Size: 99 130 63 44 183 567
Fragments arranged by size:
567 183 130 99 63 44
EcoO109I rG'GnC_Cy
Cuts at: 0 591 715 957 997 1086
Size: 591 124 242 40 89
Fragments arranged by size:
591 242 124 89 40
HaeII r_GCGC'y
Cuts at: 0 407 436 520 623 726 1086
Size: 407 29 84 103 103 360
Fragments arranged by size:
407 360 103 103 84 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 49 81 1053 1085 1086
Size: 49 32 972 32 1
Fragments arranged by size:
972 49 32 32 1
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 1086
Size: 153 933
MscI TGG'CCA
Cuts at: 0 190 421 1086
Size: 190 231 665
Fragments arranged by size:
665 231 190
MspA1I CmG'CkG
Cuts at: 0 249 450 552 657 780 887 905 1013
Size: 249 201 102 105 123 107 18 108
Cuts at: 1013 1086
Size: 73
Fragments arranged by size:
249 201 123 108 107 105 102 73
18
NaeI GCC'GGC
Cuts at: 0 171 378 727 1027 1086
Size: 171 207 349 300 59
Fragments arranged by size:
349 300 207 171 59
NgoMIV G'CCGG_C
Cuts at: 0 169 376 725 1025 1086
Size: 169 207 349 300 61
Fragments arranged by size:
349 300 207 169 61
PasI CC'CwG_GG
Cuts at: 0 639 1086
Size: 639 447
PpuMI rG'GwC_Cy
Cuts at: 0 957 1086
Size: 957 129
PspOMI G'GGCC_C
Cuts at: 0 715 1086
Size: 715 371
PstI C_TGCA'G
Cuts at: 0 779 1086
Size: 779 307
PvuII CAG'CTG
Cuts at: 0 249 780 1086
Size: 249 531 306
Fragments arranged by size:
531 306 249
SacII CC_GC'GG
Cuts at: 0 451 1014 1086
Size: 451 563 72
Fragments arranged by size:
563 451 72
SapI GCTCTTCn'nnn_
Cuts at: 0 311 538 1086
Size: 311 227 548
Fragments arranged by size:
548 311 227
SfcI C'TryA_G
Cuts at: 0 775 786 1086
Size: 775 11 300
Fragments arranged by size:
775 300 11
SgrAI Cr'CCGG_yG
Cuts at: 0 725 1086
Size: 725 361
StuI AGG'CCT
Cuts at: 0 112 1086
Size: 112 974
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 208 1086
Size: 208 878
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 466 498 1057 1086
Size: 466 32 559 29
Fragments arranged by size:
559 466 32 29
Tth111I GACn'n_nGTC
Cuts at: 0 569 1086
Size: 569 517
Enzymes that do cut:
AarI AcuI AfeI AhdI AlwNI ApaI AvaI BanII
BcgI BciVI BlpI Bme1580I BmrI BpmI BsaI BsaAI
BsaHI BsaXI BseYI BsiEI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BstEII BstXI Bsu36I BtgI BtgZI BtsI
EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I
MmeI MscI MspA1I NaeI NgoMIV PasI PpuMI PspOMI
PstI PvuII SacII SapI SfcI SgrAI StuI TaqII
TstI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BclI BfrBI
BglI BglII BmgBI BmtI BplI Bpu10I BpuEI BsaBI
BsaWI BseRI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrGI BssSI BstAPI BstBI BstYI BstZ17I ClaI
CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MslI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StyI SwaI TatI XbaI XcmI XhoI XmaI
XmnI ZraI