(Linear) (Six-base) MAPSORT of: KIBB8967.seq  Check: 7714  from: 1  to: 1068



KIBB8967.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:30  ..

Acc65I G'GTAC_C

Cuts at:      0     419    1068 
   Size:        419     649
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52    1068 
   Size:         52    1016
 
AflIII A'CryG_T

Cuts at:      0     246    1068 
   Size:        246     822
 
AleI CACnn'nnGTG

Cuts at:      0    1063    1068 
   Size:       1063       5
 
ApoI r'AATT_y

Cuts at:      0     347    1068 
   Size:        347     721
 
BanI G'GyrC_C

Cuts at:      0      54     156     419     468     789    1068 
   Size:         54     102     263      49     321     279

  Fragments arranged by size: 

                 321     279     263     102      54      49
 
BanII G_rGCy'C

Cuts at:      0     321    1068 
   Size:        321     747
 
BbeI G_GCGC'C

Cuts at:      0     793    1068 
   Size:        793     275
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     566     765    1068 
   Size:        566     199     303

  Fragments arranged by size: 

                 566     303     199
 
BglI GCCn_nnn'nGGC

Cuts at:      0     517    1068 
   Size:        517     551
 
Bme1580I G_kGCm'C

Cuts at:      0     473    1068 
   Size:        473     595
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115    1068 
   Size:        115     953
 
BsaAI yAC'GTr

Cuts at:      0     537    1068 
   Size:        537     531
 
BsaHI Gr'CG_yC

Cuts at:      0     131     790    1068 
   Size:        131     659     278

  Fragments arranged by size: 

                 659     278     131
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     718     748    1068 
   Size:        718      30     320

  Fragments arranged by size: 

                 718     320      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     477    1068 
   Size:        477     591
 
BseYI C'CCAG_C

Cuts at:      0     149     325    1068 
   Size:        149     176     743

  Fragments arranged by size: 

                 743     176     149
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1007    1068 
   Size:       1007      61
 
Bsp1286I G_dGCh'C

Cuts at:      0     321     473    1068 
   Size:        321     152     595

  Fragments arranged by size: 

                 595     321     152
 
BsrDI GCAATG_nn'

Cuts at:      0     121    1068 
   Size:        121     947
 
BsrGI T'GTAC_A

Cuts at:      0     377    1068 
   Size:        377     691
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     116     882    1068 
   Size:        116     766     186

  Fragments arranged by size: 

                 766     186     116
 
BtsI GCAGTG_nn'

Cuts at:      0     909    1068 
   Size:        909     159
 
DraI TTT'AAA

Cuts at:      0     641    1068 
   Size:        641     427
 
EaeI y'GGCC_r

Cuts at:      0      38     518    1068 
   Size:         38     480     550

  Fragments arranged by size: 

                 550     480      38
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     115    1068 
   Size:         52      63     953

  Fragments arranged by size: 

                 953      63      52
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     462    1068 
   Size:        462     606
 
HaeII r_GCGC'y

Cuts at:      0     793    1068 
   Size:        793     275
 
HindIII A'AGCT_T

Cuts at:      0     748    1068 
   Size:        748     320
 
KasI G'GCGC_C

Cuts at:      0     789    1068 
   Size:        789     279
 
KpnI G_GTAC'C

Cuts at:      0     423    1068 
   Size:        423     645
 
MfeI C'AATT_G

Cuts at:      0     396    1068 
   Size:        396     672
 
MluI A'CGCG_T

Cuts at:      0     246    1068 
   Size:        246     822
 
MscI TGG'CCA

Cuts at:      0      40     520    1068 
   Size:         40     480     548

  Fragments arranged by size: 

                 548     480      40
 
MslI CAynn'nnrTG

Cuts at:      0    1063    1068 
   Size:       1063       5
 
MspA1I CmG'CkG

Cuts at:      0     153     607     974    1068 
   Size:        153     454     367      94

  Fragments arranged by size: 

                 454     367     153      94
 
NarI GG'CG_CC

Cuts at:      0     790    1068 
   Size:        790     278
 
PasI CC'CwG_GG

Cuts at:      0    1010    1068 
   Size:       1010      58
 
PfoI T'CCnGG_A

Cuts at:      0     194    1068 
   Size:        194     874
 
PsiI TTA'TAA

Cuts at:      0     545    1068 
   Size:        545     523
 
PvuII CAG'CTG

Cuts at:      0     153     607    1068 
   Size:        153     454     461

  Fragments arranged by size: 

                 461     454     153
 
SfoI GGC'GCC

Cuts at:      0     791    1068 
   Size:        791     277
 
SspI AAT'ATT

Cuts at:      0     801    1068 
   Size:        801     267
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     922    1068 
   Size:        922     146
 
TatI w'GTAC_w

Cuts at:      0     362     377    1068 
   Size:        362      15     691

  Fragments arranged by size: 

                 691     362      15
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      48    1068 
   Size:         48    1020
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AleI     ApoI     BanI    BanII     BbeI
     BbsI     BglI Bme1580I     BpmI    BsaAI    BsaHI    BsaXI    BseRI
    BseYI     BsgI Bsp1286I    BsrDI    BsrGI    BtgZI     BtsI     DraI
     EaeI  Eco57MI     FalI    HaeII  HindIII     KasI     KpnI     MfeI
     MluI     MscI     MslI   MspA1I     NarI     PasI     PfoI     PsiI
    PvuII     SfoI     SspI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaWI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
     ClaI    CspCI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I
   HincII     HpaI     MmeI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI