Restriction map for
pFN21AB8966
(Linear) (Six-base) MAPSORT of: KIBB8966.seq Check: 1086 from: 1 to: 1068
KIBB8966.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AatII G_ACGT'C
Cuts at: 0 881 1068
Size: 881 187
AccI GT'mk_AC
Cuts at: 0 118 1068
Size: 118 950
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 1068
Size: 172 896
AflII C'TTAA_G
Cuts at: 0 765 1068
Size: 765 303
AlwNI CAG_nnn'CTG
Cuts at: 0 465 1068
Size: 465 603
ApoI r'AATT_y
Cuts at: 0 3 10 481 1068
Size: 3 7 471 587
Fragments arranged by size:
587 471 7 3
AseI AT'TA_AT
Cuts at: 0 124 1068
Size: 124 944
AvaI C'yCGr_G
Cuts at: 0 277 642 693 859 885 1068
Size: 277 365 51 166 26 183
Fragments arranged by size:
365 277 183 166 51 26
BanI G'GyrC_C
Cuts at: 0 175 753 890 1068
Size: 175 578 137 178
Fragments arranged by size:
578 178 175 137
BanII G_rGCy'C
Cuts at: 0 651 860 1068
Size: 651 209 208
Fragments arranged by size:
651 209 208
BbeI G_GCGC'C
Cuts at: 0 179 894 1068
Size: 179 715 174
Fragments arranged by size:
715 179 174
BbsI GAAGACnn'nnnn_
Cuts at: 0 873 881 1068
Size: 873 8 187
Fragments arranged by size:
873 187 8
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 797 831 1068
Size: 797 34 237
Fragments arranged by size:
797 237 34
BglI GCCn_nnn'nGGC
Cuts at: 0 271 364 698 715 1068
Size: 271 93 334 17 353
Fragments arranged by size:
353 334 271 93 17
BmgBI CAC'GTC
Cuts at: 0 335 1068
Size: 335 733
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 493 673 874 931 1068
Size: 151 342 180 201 57 137
Fragments arranged by size:
342 201 180 151 137 57
BsaI GGTCTCn'nnnn_
Cuts at: 0 186 1068
Size: 186 882
BsaHI Gr'CG_yC
Cuts at: 0 176 878 891 1068
Size: 176 702 13 177
Fragments arranged by size:
702 177 176 13
BsaWI w'CCGG_w
Cuts at: 0 955 1068
Size: 955 113
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 13 43 1068
Size: 13 30 1025
Fragments arranged by size:
1025 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 122 125 128 1068
Size: 122 3 3 940
Fragments arranged by size:
940 122 3 3
BseYI C'CCAG_C
Cuts at: 0 160 319 1068
Size: 160 159 749
Fragments arranged by size:
749 160 159
BsiEI CG_ry'CG
Cuts at: 0 350 935 1068
Size: 350 585 133
Fragments arranged by size:
585 350 133
BsmBI CGTCTCn'nnnn_
Cuts at: 0 341 1068
Size: 341 727
Bsp1286I G_dGCh'C
Cuts at: 0 651 860 1068
Size: 651 209 208
Fragments arranged by size:
651 209 208
BspEI T'CCGG_A
Cuts at: 0 955 1068
Size: 955 113
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 546 1064 1068
Size: 546 518 4
Fragments arranged by size:
546 518 4
BsrDI GCAATG_nn'
Cuts at: 0 865 1068
Size: 865 203
BsrFI r'CCGG_y
Cuts at: 0 506 578 844 1068
Size: 506 72 266 224
Fragments arranged by size:
506 266 224 72
BssHII G'CGCG_C
Cuts at: 0 427 830 1068
Size: 427 403 238
Fragments arranged by size:
427 403 238
BtgI C'CryG_G
Cuts at: 0 683 1068
Size: 683 385
DrdI GACnn_nn'nnGTC
Cuts at: 0 997 1068
Size: 997 71
EaeI y'GGCC_r
Cuts at: 0 347 508 686 932 1068
Size: 347 161 178 246 136
Fragments arranged by size:
347 246 178 161 136
EagI C'GGCC_G
Cuts at: 0 347 932 1068
Size: 347 585 136
Fragments arranged by size:
585 347 136
EarI CTCTTCn'nnn_
Cuts at: 0 1010 1068
Size: 1010 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 297 318 345 696 1068
Size: 297 21 27 351 372
Fragments arranged by size:
372 351 297 27 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 172 493 673 874 931 1068
Size: 151 21 321 180 201 57 137
Fragments arranged by size:
321 201 180 151 137 57 21
EcoO109I rG'GnC_Cy
Cuts at: 0 761 1068
Size: 761 307
EcoRI G'AATT_C
Cuts at: 0 481 1068
Size: 481 587
FspI TGC'GCA
Cuts at: 0 439 1068
Size: 439 629
HaeII r_GCGC'y
Cuts at: 0 179 894 1068
Size: 179 715 174
Fragments arranged by size:
715 179 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 868 900 1068
Size: 289 32 547 32 168
Fragments arranged by size:
547 289 168 32 32
HincII GTy'rAC
Cuts at: 0 119 1068
Size: 119 949
KasI G'GCGC_C
Cuts at: 0 175 890 1068
Size: 175 715 178
Fragments arranged by size:
715 178 175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 1068
Size: 357 711
MspA1I CmG'CkG
Cuts at: 0 79 262 462 685 1068
Size: 79 183 200 223 383
Fragments arranged by size:
383 223 200 183 79
NaeI GCC'GGC
Cuts at: 0 508 846 1068
Size: 508 338 222
Fragments arranged by size:
508 338 222
NarI GG'CG_CC
Cuts at: 0 176 891 1068
Size: 176 715 177
Fragments arranged by size:
715 177 176
NgoMIV G'CCGG_C
Cuts at: 0 506 844 1068
Size: 506 338 224
Fragments arranged by size:
506 338 224
PshAI GACnn'nnGTC
Cuts at: 0 941 1068
Size: 941 127
PsiI TTA'TAA
Cuts at: 0 34 1068
Size: 34 1034
PstI C_TGCA'G
Cuts at: 0 390 1061 1068
Size: 390 671 7
Fragments arranged by size:
671 390 7
PvuII CAG'CTG
Cuts at: 0 462 1068
Size: 462 606
SacII CC_GC'GG
Cuts at: 0 686 1068
Size: 686 382
SalI G'TCGA_C
Cuts at: 0 117 1068
Size: 117 951
SapI GCTCTTCn'nnn_
Cuts at: 0 1010 1068
Size: 1010 58
SfcI C'TryA_G
Cuts at: 0 386 976 1057 1068
Size: 386 590 81 11
Fragments arranged by size:
590 386 81 11
SfoI GGC'GCC
Cuts at: 0 177 892 1068
Size: 177 715 176
Fragments arranged by size:
715 177 176
SmaI CCC'GGG
Cuts at: 0 644 695 861 1068
Size: 644 51 166 207
Fragments arranged by size:
644 207 166 51
SmlI C'TyrA_G
Cuts at: 0 765 1068
Size: 765 303
SrfI GCCC'GGGC
Cuts at: 0 695 861 1068
Size: 695 166 207
Fragments arranged by size:
695 207 166
StuI AGG'CCT
Cuts at: 0 962 1068
Size: 962 106
StyI C'CwwG_G
Cuts at: 0 286 1068
Size: 286 782
XmaI C'CCGG_G
Cuts at: 0 642 693 859 1068
Size: 642 51 166 209
Fragments arranged by size:
642 209 166 51
XmnI GAAnn'nnTTC
Cuts at: 0 481 1068
Size: 481 587
ZraI GAC'GTC
Cuts at: 0 879 1068
Size: 879 189
Enzymes that do cut:
AatII AccI AcuI AflII AlwNI ApoI AseI AvaI
BanI BanII BbeI BbsI BcgI BglI BmgBI BpmI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsmBI
Bsp1286I BspEI BspMI BsrDI BsrFI BssHII BtgI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII KasI MmeI MspA1I NaeI NarI
NgoMIV PshAI PsiI PstI PvuII SacII SalI SapI
SfcI SfoI SmaI SmlI SrfI StuI StyI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsgI
BsiHKAI BsiWI BsmI BspHI BsrBI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI HindIII HpaI KpnI MfeI MluI MscI
MslI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PspXI PsrI PvuI RsrII SacI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SspI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI