Restriction map for
pFN21AB8954
(Linear) (Six-base) MAPSORT of: KIBB8954.seq Check: 8942 from: 1 to: 990
KIBB8954.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 990
Size: 835 155
AflIII A'CryG_T
Cuts at: 0 569 789 990
Size: 569 220 201
Fragments arranged by size:
569 220 201
AhdI GACnn_n'nnGTC
Cuts at: 0 492 990
Size: 492 498
AleI CACnn'nnGTG
Cuts at: 0 805 990
Size: 805 185
AlwNI CAG_nnn'CTG
Cuts at: 0 188 203 990
Size: 188 15 787
Fragments arranged by size:
787 188 15
AscI GG'CGCG_CC
Cuts at: 0 304 990
Size: 304 686
AvaI C'yCGr_G
Cuts at: 0 162 444 583 754 990
Size: 162 282 139 171 236
Fragments arranged by size:
282 236 171 162 139
BanI G'GyrC_C
Cuts at: 0 256 268 472 579 596 990
Size: 256 12 204 107 17 394
Fragments arranged by size:
394 256 204 107 17 12
BanII G_rGCy'C
Cuts at: 0 151 654 990
Size: 151 503 336
Fragments arranged by size:
503 336 151
BbeI G_GCGC'C
Cuts at: 0 260 272 476 990
Size: 260 12 204 514
Fragments arranged by size:
514 260 204 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 28 990
Size: 28 962
BglI GCCn_nnn'nGGC
Cuts at: 0 298 990
Size: 298 692
Bme1580I G_kGCm'C
Cuts at: 0 318 584 599 797 990
Size: 318 266 15 198 193
Fragments arranged by size:
318 266 198 193 15
BmgBI CAC'GTC
Cuts at: 0 570 738 990
Size: 570 168 252
Fragments arranged by size:
570 252 168
BmrI ACTGGGnnnn_n'
Cuts at: 0 657 990
Size: 657 333
BmtI G_CTAG'C
Cuts at: 0 845 990
Size: 845 145
BsaHI Gr'CG_yC
Cuts at: 0 257 269 473 617 990
Size: 257 12 204 144 373
Fragments arranged by size:
373 257 204 144 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 113 116 990
Size: 113 3 874
Fragments arranged by size:
874 113 3
BseYI C'CCAG_C
Cuts at: 0 231 526 665 990
Size: 231 295 139 325
Fragments arranged by size:
325 295 231 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 990
Size: 829 161
Bsp1286I G_dGCh'C
Cuts at: 0 151 318 584 599 654 797 990
Size: 151 167 266 15 55 143 193
Fragments arranged by size:
266 193 167 151 143 55 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 221 425 990
Size: 221 204 565
Fragments arranged by size:
565 221 204
BsrBI CCG'CTC
Cuts at: 0 77 124 990
Size: 77 47 866
Fragments arranged by size:
866 77 47
BsrFI r'CCGG_y
Cuts at: 0 243 770 869 929 990
Size: 243 527 99 60 61
Fragments arranged by size:
527 243 99 61 60
BssHII G'CGCG_C
Cuts at: 0 304 990
Size: 304 686
BstAPI GCAn_nnn'nTGC
Cuts at: 0 188 228 990
Size: 188 40 762
Fragments arranged by size:
762 188 40
BstEII G'GTnAC_C
Cuts at: 0 909 990
Size: 909 81
Bsu36I CC'TnA_GG
Cuts at: 0 263 990
Size: 263 727
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 565 990
Size: 565 425
BtsI GCAGTG_nn'
Cuts at: 0 201 990
Size: 201 789
DraIII CAC_nnn'GTG
Cuts at: 0 228 411 990
Size: 228 183 579
Fragments arranged by size:
579 228 183
EaeI y'GGCC_r
Cuts at: 0 31 194 990
Size: 31 163 796
Fragments arranged by size:
796 163 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 85 110 134 696 870 990
Size: 85 25 24 562 174 120
Fragments arranged by size:
562 174 120 85 25 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 990
Size: 835 155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 744 990
Size: 744 246
HaeII r_GCGC'y
Cuts at: 0 260 272 350 476 870 906 973 990
Size: 260 12 78 126 394 36 67 17
Fragments arranged by size:
394 260 126 78 67 36 17 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 478 481 510 513 990
Size: 478 3 29 3 477
Fragments arranged by size:
478 477 29 3 3
KasI G'GCGC_C
Cuts at: 0 256 268 472 990
Size: 256 12 204 518
Fragments arranged by size:
518 256 204 12
MluI A'CGCG_T
Cuts at: 0 789 990
Size: 789 201
MslI CAynn'nnrTG
Cuts at: 0 805 990
Size: 805 185
MspA1I CmG'CkG
Cuts at: 0 140 188 593 813 990
Size: 140 48 405 220 177
Fragments arranged by size:
405 220 177 140 48
NaeI GCC'GGC
Cuts at: 0 245 871 990
Size: 245 626 119
Fragments arranged by size:
626 245 119
NarI GG'CG_CC
Cuts at: 0 257 269 473 990
Size: 257 12 204 517
Fragments arranged by size:
517 257 204 12
NgoMIV G'CCGG_C
Cuts at: 0 243 869 990
Size: 243 626 121
Fragments arranged by size:
626 243 121
NheI G'CTAG_C
Cuts at: 0 841 990
Size: 841 149
PasI CC'CwG_GG
Cuts at: 0 252 354 990
Size: 252 102 636
Fragments arranged by size:
636 252 102
PflMI CCAn_nnn'nTGG
Cuts at: 0 665 990
Size: 665 325
PstI C_TGCA'G
Cuts at: 0 139 422 752 990
Size: 139 283 330 238
Fragments arranged by size:
330 283 238 139
PvuII CAG'CTG
Cuts at: 0 188 813 990
Size: 188 625 177
Fragments arranged by size:
625 188 177
SfcI C'TryA_G
Cuts at: 0 135 418 748 990
Size: 135 283 330 242
Fragments arranged by size:
330 283 242 135
SfoI GGC'GCC
Cuts at: 0 258 270 474 990
Size: 258 12 204 516
Fragments arranged by size:
516 258 204 12
SgrAI Cr'CCGG_yG
Cuts at: 0 869 990
Size: 869 121
SmaI CCC'GGG
Cuts at: 0 446 585 990
Size: 446 139 405
Fragments arranged by size:
446 405 139
StyI C'CwwG_G
Cuts at: 0 506 689 990
Size: 506 183 301
Fragments arranged by size:
506 301 183
TatI w'GTAC_w
Cuts at: 0 680 990
Size: 680 310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 351 990
Size: 351 639
XmaI C'CCGG_G
Cuts at: 0 444 583 990
Size: 444 139 407
Fragments arranged by size:
444 407 139
Enzymes that do cut:
AcuI AflIII AhdI AleI AlwNI AscI AvaI BanI
BanII BbeI BcgI BglI Bme1580I BmgBI BmrI BmtI
BsaHI BseRI BseYI BsgI Bsp1286I BspMI BsrBI BsrFI
BssHII BstAPI BstEII Bsu36I BtgZI BtsI DraIII EaeI
EciI Eco57MI FalI HaeII Hin4I KasI MluI MslI
MspA1I NaeI NarI NgoMIV NheI PasI PflMI PstI
PvuII SfcI SfoI SgrAI SmaI StyI TatI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI ApaI ApaLI ApoI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssSI BstBI BstXI BstYI BstZ17I
BtgI ClaI CspCI DraI DrdI EagI EarI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MmeI MscI NcoI NdeI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XhoI XmnI ZraI