Restriction map for
pFN21AB8933
(Linear) (Six-base) MAPSORT of: KIBB8933.seq Check: 3486 from: 1 to: 852
KIBB8933.orf
With 182 enzymes: SgfI *
March 22, 2012 15:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 134 852
Size: 134 718
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 852
Size: 283 569
AhdI GACnn_n'nnGTC
Cuts at: 0 846 852
Size: 846 6
AlwNI CAG_nnn'CTG
Cuts at: 0 255 386 509 852
Size: 255 131 123 343
Fragments arranged by size:
343 255 131 123
ApoI r'AATT_y
Cuts at: 0 340 655 852
Size: 340 315 197
Fragments arranged by size:
340 315 197
AvaI C'yCGr_G
Cuts at: 0 744 852
Size: 744 108
BanI G'GyrC_C
Cuts at: 0 310 852
Size: 310 542
BanII G_rGCy'C
Cuts at: 0 197 730 852
Size: 197 533 122
Fragments arranged by size:
533 197 122
BbeI G_GCGC'C
Cuts at: 0 314 852
Size: 314 538
BglI GCCn_nnn'nGGC
Cuts at: 0 547 852
Size: 547 305
BmrI ACTGGGnnnn_n'
Cuts at: 0 761 852
Size: 761 91
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 675 852
Size: 675 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 229 813 852
Size: 100 129 584 39
Fragments arranged by size:
584 129 100 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 852
Size: 133 719
BsaI GGTCTCn'nnnn_
Cuts at: 0 370 527 852
Size: 370 157 325
Fragments arranged by size:
370 325 157
BsaAI yAC'GTr
Cuts at: 0 276 852
Size: 276 576
BsaHI Gr'CG_yC
Cuts at: 0 311 852
Size: 311 541
BsaWI w'CCGG_w
Cuts at: 0 521 852
Size: 521 331
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 565 571 595 601 852
Size: 565 6 24 6 251
Fragments arranged by size:
565 251 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 388 573 576 602 852
Size: 388 185 3 26 250
Fragments arranged by size:
388 250 185 26 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 852
Size: 112 740
BsiEI CG_ry'CG
Cuts at: 0 300 852
Size: 300 552
BsiHKAI G_wGCw'C
Cuts at: 0 197 730 852
Size: 197 533 122
Fragments arranged by size:
533 197 122
Bsp1286I G_dGCh'C
Cuts at: 0 197 730 852
Size: 197 533 122
Fragments arranged by size:
533 197 122
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 83 134 852
Size: 83 51 718
Fragments arranged by size:
718 83 51
BsrBI CCG'CTC
Cuts at: 0 633 852
Size: 633 219
BsrFI r'CCGG_y
Cuts at: 0 481 852
Size: 481 371
BstXI CCAn_nnnn'nTGG
Cuts at: 0 162 837 852
Size: 162 675 15
Fragments arranged by size:
675 162 15
BstYI r'GATC_y
Cuts at: 0 203 852
Size: 203 649
BtgI C'CryG_G
Cuts at: 0 177 314 542 852
Size: 177 137 228 310
Fragments arranged by size:
310 228 177 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 431 852
Size: 431 421
BtsI GCAGTG_nn'
Cuts at: 0 106 346 852
Size: 106 240 506
Fragments arranged by size:
506 240 106
EaeI y'GGCC_r
Cuts at: 0 297 470 852
Size: 297 173 382
Fragments arranged by size:
382 297 173
EagI C'GGCC_G
Cuts at: 0 297 852
Size: 297 555
EarI CTCTTCn'nnn_
Cuts at: 0 644 852
Size: 644 208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 86 852
Size: 86 766
EcoICRI GAG'CTC
Cuts at: 0 195 728 852
Size: 195 533 124
Fragments arranged by size:
533 195 124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 229 283 813 852
Size: 100 129 54 530 39
Fragments arranged by size:
530 129 100 54 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 617 852
Size: 617 235
EcoRI G'AATT_C
Cuts at: 0 655 852
Size: 655 197
HaeII r_GCGC'y
Cuts at: 0 87 314 852
Size: 87 227 538
Fragments arranged by size:
538 227 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 670 702 832 852
Size: 670 32 130 20
Fragments arranged by size:
670 130 32 20
KasI G'GCGC_C
Cuts at: 0 310 852
Size: 310 542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 852
Size: 614 238
MspA1I CmG'CkG
Cuts at: 0 179 544 852
Size: 179 365 308
Fragments arranged by size:
365 308 179
NarI GG'CG_CC
Cuts at: 0 311 852
Size: 311 541
PasI CC'CwG_GG
Cuts at: 0 306 852
Size: 306 546
PfoI T'CCnGG_A
Cuts at: 0 206 852
Size: 206 646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 592 624 852
Size: 592 32 228
Fragments arranged by size:
592 228 32
PstI C_TGCA'G
Cuts at: 0 65 251 704 782 852
Size: 65 186 453 78 70
Fragments arranged by size:
453 186 78 70 65
SacI G_AGCT'C
Cuts at: 0 197 730 852
Size: 197 533 122
Fragments arranged by size:
533 197 122
SacII CC_GC'GG
Cuts at: 0 180 545 852
Size: 180 365 307
Fragments arranged by size:
365 307 180
SfcI C'TryA_G
Cuts at: 0 61 247 700 778 852
Size: 61 186 453 78 74
Fragments arranged by size:
453 186 78 74 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 547 852
Size: 547 305
SfoI GGC'GCC
Cuts at: 0 312 852
Size: 312 540
SmaI CCC'GGG
Cuts at: 0 746 852
Size: 746 106
SmlI C'TyrA_G
Cuts at: 0 148 852
Size: 148 704
StyI C'CwwG_G
Cuts at: 0 155 551 852
Size: 155 396 301
Fragments arranged by size:
396 301 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 511 678 852
Size: 511 167 174
Fragments arranged by size:
511 174 167
XmaI C'CCGG_G
Cuts at: 0 744 852
Size: 744 108
Enzymes that do cut:
AarI AcuI AhdI AlwNI ApoI AvaI BanI BanII
BbeI BglI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BstXI BstYI BtgI BtgZI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
HaeII Hin4I KasI MmeI MspA1I NarI PasI PfoI
PpiI PstI SacI SacII SfcI SfiI SfoI SmaI
SmlI StyI TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmtI Bpu10I BsaBI
BseYI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EcoO109I EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MscI MslI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SalI SanDI SapI SbfI ScaI SexAI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI