Restriction map for
pFN21AB8927
(Linear) (Six-base) MAPSORT of: KIBB8927.seq Check: 7025 from: 1 to: 819
KIBB8927.orf
With 182 enzymes: SgfI *
March 22, 2012 15:45 ..
Acc65I G'GTAC_C
Cuts at: 0 268 819
Size: 268 551
AfeI AGC'GCT
Cuts at: 0 514 819
Size: 514 305
AflIII A'CryG_T
Cuts at: 0 656 819
Size: 656 163
AlwNI CAG_nnn'CTG
Cuts at: 0 672 819
Size: 672 147
ApaI G_GGCC'C
Cuts at: 0 88 232 298 819
Size: 88 144 66 521
Fragments arranged by size:
521 144 88 66
AvaI C'yCGr_G
Cuts at: 0 291 297 490 762 819
Size: 291 6 193 272 57
Fragments arranged by size:
291 272 193 57 6
BanI G'GyrC_C
Cuts at: 0 96 183 268 723 819
Size: 96 87 85 455 96
Fragments arranged by size:
455 96 96 87 85
BanII G_rGCy'C
Cuts at: 0 88 110 232 298 382 819
Size: 88 22 122 66 84 437
Fragments arranged by size:
437 122 88 84 66 22
BbeI G_GCGC'C
Cuts at: 0 187 819
Size: 187 632
BbsI GAAGACnn'nnnn_
Cuts at: 0 457 819
Size: 457 362
BglI GCCn_nnn'nGGC
Cuts at: 0 302 439 649 819
Size: 302 137 210 170
Fragments arranged by size:
302 210 170 137
BlpI GC'TnA_GC
Cuts at: 0 422 629 819
Size: 422 207 190
Fragments arranged by size:
422 207 190
Bme1580I G_kGCm'C
Cuts at: 0 88 232 298 819
Size: 88 144 66 521
Fragments arranged by size:
521 144 88 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 534 655 819
Size: 36 498 121 164
Fragments arranged by size:
498 164 121 36
Bpu10I CC'TnA_GC
Cuts at: 0 520 819
Size: 520 299
BsaI GGTCTCn'nnnn_
Cuts at: 0 562 819
Size: 562 257
BsaHI Gr'CG_yC
Cuts at: 0 47 184 819
Size: 47 137 635
Fragments arranged by size:
635 137 47
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 172 175 819
Size: 169 3 3 644
Fragments arranged by size:
644 169 3 3
BsiEI CG_ry'CG
Cuts at: 0 41 406 819
Size: 41 365 413
Fragments arranged by size:
413 365 41
BsmBI CGTCTCn'nnnn_
Cuts at: 0 54 819
Size: 54 765
Bsp1286I G_dGCh'C
Cuts at: 0 88 110 232 298 382 819
Size: 88 22 122 66 84 437
Fragments arranged by size:
437 122 88 84 66 22
BsrBI CCG'CTC
Cuts at: 0 402 426 753 819
Size: 402 24 327 66
Fragments arranged by size:
402 327 66 24
BsrFI r'CCGG_y
Cuts at: 0 40 433 720 819
Size: 40 393 287 99
Fragments arranged by size:
393 287 99 40
BssHII G'CGCG_C
Cuts at: 0 534 819
Size: 534 285
Bsu36I CC'TnA_GG
Cuts at: 0 218 819
Size: 218 601
BtgI C'CryG_G
Cuts at: 0 100 374 406 819
Size: 100 274 32 413
Fragments arranged by size:
413 274 100 32
DraI TTT'AAA
Cuts at: 0 348 819
Size: 348 471
EaeI y'GGCC_r
Cuts at: 0 38 118 403 440 641 819
Size: 38 80 285 37 201 178
Fragments arranged by size:
285 201 178 80 38 37
EagI C'GGCC_G
Cuts at: 0 38 403 819
Size: 38 365 416
Fragments arranged by size:
416 365 38
EarI CTCTTCn'nnn_
Cuts at: 0 347 819
Size: 347 472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 534 655 819
Size: 36 498 121 164
Fragments arranged by size:
498 164 121 36
EcoO109I rG'GnC_Cy
Cuts at: 0 84 85 228 229 819
Size: 84 1 143 1 590
Fragments arranged by size:
590 143 84 1 1
HaeII r_GCGC'y
Cuts at: 0 187 422 516 819
Size: 187 235 94 303
Fragments arranged by size:
303 235 187 94
KasI G'GCGC_C
Cuts at: 0 183 819
Size: 183 636
KpnI G_GTAC'C
Cuts at: 0 272 819
Size: 272 547
MluI A'CGCG_T
Cuts at: 0 656 819
Size: 656 163
MscI TGG'CCA
Cuts at: 0 120 819
Size: 120 699
MspA1I CmG'CkG
Cuts at: 0 102 284 376 408 687 819
Size: 102 182 92 32 279 132
Fragments arranged by size:
279 182 132 102 92 32
NaeI GCC'GGC
Cuts at: 0 435 819
Size: 435 384
NarI GG'CG_CC
Cuts at: 0 184 819
Size: 184 635
NgoMIV G'CCGG_C
Cuts at: 0 433 819
Size: 433 386
NotI GC'GGCC_GC
Cuts at: 0 403 819
Size: 403 416
NspI r_CATG'y
Cuts at: 0 684 819
Size: 684 135
PfoI T'CCnGG_A
Cuts at: 0 695 819
Size: 695 124
PspOMI G'GGCC_C
Cuts at: 0 84 228 294 819
Size: 84 144 66 525
Fragments arranged by size:
525 144 84 66
PspXI vC'TCGA_Gb
Cuts at: 0 490 819
Size: 490 329
PstI C_TGCA'G
Cuts at: 0 560 819
Size: 560 259
SacII CC_GC'GG
Cuts at: 0 103 377 409 819
Size: 103 274 32 410
Fragments arranged by size:
410 274 103 32
SfcI C'TryA_G
Cuts at: 0 556 819
Size: 556 263
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 439 819
Size: 439 380
SfoI GGC'GCC
Cuts at: 0 185 819
Size: 185 634
SmaI CCC'GGG
Cuts at: 0 299 764 819
Size: 299 465 55
Fragments arranged by size:
465 299 55
SmlI C'TyrA_G
Cuts at: 0 490 819
Size: 490 329
SphI G_CATG'C
Cuts at: 0 684 819
Size: 684 135
SrfI GCCC'GGGC
Cuts at: 0 299 819
Size: 299 520
StyI C'CwwG_G
Cuts at: 0 275 819
Size: 275 544
XhoI C'TCGA_G
Cuts at: 0 490 819
Size: 490 329
XmaI C'CCGG_G
Cuts at: 0 297 762 819
Size: 297 465 57
Fragments arranged by size:
465 297 57
Enzymes that do cut:
Acc65I AfeI AflIII AlwNI ApaI AvaI BanI BanII
BbeI BbsI BglI BlpI Bme1580I BpmI Bpu10I BsaI
BsaHI BseRI BsiEI BsmBI Bsp1286I BsrBI BsrFI BssHII
Bsu36I BtgI DraI EaeI EagI EarI Eco57MI EcoO109I
HaeII KasI KpnI MluI MscI MspA1I NaeI NarI
NgoMIV NotI NspI PfoI PspOMI PspXI PstI SacII
SfcI SfiI SfoI SmaI SmlI SphI SrfI StyI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI
BsaWI BsaXI BseYI BsgI BsiHKAI BsiWI BsmI BspEI
BspHI BspMI BsrDI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI DraIII
DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI MfeI MmeI
MslI NcoI NdeI NheI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XmnI ZraI