Restriction map for
pFN21AB8921
(Linear) (Six-base) MAPSORT of: KIBB8921.seq Check: 8807 from: 1 to: 801
KIBB8921.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 406 801
Size: 406 395
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 642 801
Size: 123 519 159
Fragments arranged by size:
519 159 123
AleI CACnn'nnGTG
Cuts at: 0 772 801
Size: 772 29
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 463 495 801
Size: 463 32 306
Fragments arranged by size:
463 306 32
ApaI G_GGCC'C
Cuts at: 0 52 801
Size: 52 749
ApoI r'AATT_y
Cuts at: 0 321 801
Size: 321 480
AvaI C'yCGr_G
Cuts at: 0 75 257 274 801
Size: 75 182 17 527
Fragments arranged by size:
527 182 75 17
BanI G'GyrC_C
Cuts at: 0 88 801
Size: 88 713
BanII G_rGCy'C
Cuts at: 0 52 391 801
Size: 52 339 410
Fragments arranged by size:
410 339 52
BbeI G_GCGC'C
Cuts at: 0 92 801
Size: 92 709
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 238 272 579 613 801
Size: 238 34 307 34 188
Fragments arranged by size:
307 238 188 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 97 801
Size: 97 704
Bme1580I G_kGCm'C
Cuts at: 0 52 251 801
Size: 52 199 550
Fragments arranged by size:
550 199 52
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 321 801
Size: 321 480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 490 510 636 801
Size: 111 379 20 126 165
Fragments arranged by size:
379 165 126 111 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 129 292 541 801
Size: 129 163 249 260
Fragments arranged by size:
260 249 163 129
BsaHI Gr'CG_yC
Cuts at: 0 89 184 801
Size: 89 95 617
Fragments arranged by size:
617 95 89
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 463 493 801
Size: 463 30 308
Fragments arranged by size:
463 308 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 121 451 801
Size: 58 63 330 350
Fragments arranged by size:
350 330 63 58
BseYI C'CCAG_C
Cuts at: 0 51 455 801
Size: 51 404 346
Fragments arranged by size:
404 346 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 536 801
Size: 384 152 265
Fragments arranged by size:
384 265 152
BsiEI CG_ry'CG
Cuts at: 0 200 215 360 801
Size: 200 15 145 441
Fragments arranged by size:
441 200 145 15
Bsp1286I G_dGCh'C
Cuts at: 0 52 251 391 801
Size: 52 199 140 410
Fragments arranged by size:
410 199 140 52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 406 801
Size: 406 395
BsrBI CCG'CTC
Cuts at: 0 337 801
Size: 337 464
BsrDI GCAATG_nn'
Cuts at: 0 27 503 801
Size: 27 476 298
Fragments arranged by size:
476 298 27
BsrFI r'CCGG_y
Cuts at: 0 195 801
Size: 195 606
BstAPI GCAn_nnn'nTGC
Cuts at: 0 514 801
Size: 514 287
BstXI CCAn_nnnn'nTGG
Cuts at: 0 774 801
Size: 774 27
Bsu36I CC'TnA_GG
Cuts at: 0 406 801
Size: 406 395
BtgI C'CryG_G
Cuts at: 0 101 801
Size: 101 700
BtsI GCAGTG_nn'
Cuts at: 0 62 232 801
Size: 62 170 569
Fragments arranged by size:
569 170 62
EaeI y'GGCC_r
Cuts at: 0 62 197 212 229 357 801
Size: 62 135 15 17 128 444
Fragments arranged by size:
444 135 128 62 17 15
EagI C'GGCC_G
Cuts at: 0 197 212 357 801
Size: 197 15 145 444
Fragments arranged by size:
444 197 145 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 123 490 510 636 642 801
Size: 111 12 367 20 126 6 159
Fragments arranged by size:
367 159 126 111 20 12 6
EcoO109I rG'GnC_Cy
Cuts at: 0 48 79 801
Size: 48 31 722
Fragments arranged by size:
722 48 31
HaeII r_GCGC'y
Cuts at: 0 92 114 154 209 257 801
Size: 92 22 40 55 48 544
Fragments arranged by size:
544 92 55 48 40 22
KasI G'GCGC_C
Cuts at: 0 88 801
Size: 88 713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 296 801
Size: 185 111 505
Fragments arranged by size:
505 185 111
MslI CAynn'nnrTG
Cuts at: 0 772 801
Size: 772 29
MspA1I CmG'CkG
Cuts at: 0 103 192 282 801
Size: 103 89 90 519
Fragments arranged by size:
519 103 90 89
NaeI GCC'GGC
Cuts at: 0 197 801
Size: 197 604
NarI GG'CG_CC
Cuts at: 0 89 801
Size: 89 712
NgoMIV G'CCGG_C
Cuts at: 0 195 801
Size: 195 606
NspI r_CATG'y
Cuts at: 0 789 801
Size: 789 12
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 801
Size: 463 32 306
Fragments arranged by size:
463 306 32
PshAI GACnn'nnGTC
Cuts at: 0 553 801
Size: 553 248
PspOMI G'GGCC_C
Cuts at: 0 48 801
Size: 48 753
PstI C_TGCA'G
Cuts at: 0 602 692 801
Size: 602 90 109
Fragments arranged by size:
602 109 90
SacII CC_GC'GG
Cuts at: 0 104 801
Size: 104 697
SbfI CC_TGCA'GG
Cuts at: 0 692 801
Size: 692 109
SfcI C'TryA_G
Cuts at: 0 598 688 801
Size: 598 90 113
Fragments arranged by size:
598 113 90
SfoI GGC'GCC
Cuts at: 0 90 801
Size: 90 711
SmaI CCC'GGG
Cuts at: 0 77 276 801
Size: 77 199 525
Fragments arranged by size:
525 199 77
StyI C'CwwG_G
Cuts at: 0 176 584 801
Size: 176 408 217
Fragments arranged by size:
408 217 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 297 801
Size: 297 504
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 750 801
Size: 750 51
XmaI C'CCGG_G
Cuts at: 0 75 274 801
Size: 75 199 527
Fragments arranged by size:
527 199 75
XmnI GAAnn'nnTTC
Cuts at: 0 292 801
Size: 292 509
Enzymes that do cut:
AarI AcuI AleI AloI ApaI ApoI AvaI BanI
BanII BbeI BcgI BglI Bme1580I BplI BpmI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BspMI
BsrBI BsrDI BsrFI BstAPI BstXI Bsu36I BtgI BtsI
EaeI EagI Eco57MI EcoO109I HaeII KasI MmeI MslI
MspA1I NaeI NarI NgoMIV NspI PpiI PshAI PspOMI
PstI SacII SbfI SfcI SfoI SmaI StyI TaqII
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaBI
BsaWI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BssHII BssSI BstBI BstEII BstYI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpuMI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SalI SanDI SapI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XhoI
ZraI