Restriction map for
pFN21AB8912
(Linear) (Six-base) MAPSORT of: KIBB8912.seq Check: 755 from: 1 to: 753
KIBB8912.orf
With 182 enzymes: SgfI *
March 22, 2012 15:44 ..
AccI GT'mk_AC
Cuts at: 0 38 422 753
Size: 38 384 331
Fragments arranged by size:
384 331 38
AfeI AGC'GCT
Cuts at: 0 190 325 490 753
Size: 190 135 165 263
Fragments arranged by size:
263 190 165 135
ApaI G_GGCC'C
Cuts at: 0 218 303 719 753
Size: 218 85 416 34
Fragments arranged by size:
416 218 85 34
ApaLI G'TGCA_C
Cuts at: 0 177 753
Size: 177 576
ApoI r'AATT_y
Cuts at: 0 538 753
Size: 538 215
AvaI C'yCGr_G
Cuts at: 0 103 272 687 753
Size: 103 169 415 66
Fragments arranged by size:
415 169 103 66
BanI G'GyrC_C
Cuts at: 0 566 753
Size: 566 187
BanII G_rGCy'C
Cuts at: 0 218 279 303 317 416 485 719 753
Size: 218 61 24 14 99 69 234 34
Fragments arranged by size:
234 218 99 69 61 34 24 14
BbeI G_GCGC'C
Cuts at: 0 570 753
Size: 570 183
BclI T'GATC_A
Cuts at: 0 20 753
Size: 20 733
BglII A'GATC_T
Cuts at: 0 620 753
Size: 620 133
BlpI GC'TnA_GC
Cuts at: 0 705 753
Size: 705 48
Bme1580I G_kGCm'C
Cuts at: 0 181 218 303 719 753
Size: 181 37 85 416 34
Fragments arranged by size:
416 181 85 37 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 753
Size: 550 203
BsaAI yAC'GTr
Cuts at: 0 447 753
Size: 447 306
BsaBI GATnn'nnATC
Cuts at: 0 619 753
Size: 619 134
BsaHI Gr'CG_yC
Cuts at: 0 567 753
Size: 567 186
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 295 753
Size: 295 458
BseYI C'CCAG_C
Cuts at: 0 88 385 753
Size: 88 297 368
Fragments arranged by size:
368 297 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 364 753
Size: 205 159 389
Fragments arranged by size:
389 205 159
BsiHKAI G_wGCw'C
Cuts at: 0 181 454 753
Size: 181 273 299
Fragments arranged by size:
299 273 181
Bsp1286I G_dGCh'C
Cuts at: 0 181 218 279 303 317 416 454 485
Size: 181 37 61 24 14 99 38 31
Cuts at: 485 719 753
Size: 234 34
Fragments arranged by size:
234 181 99 61 38 37 34 31
24 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 507 753
Size: 507 246
BsrBI CCG'CTC
Cuts at: 0 686 753
Size: 686 67
BsrFI r'CCGG_y
Cuts at: 0 115 472 635 725 753
Size: 115 357 163 90 28
Fragments arranged by size:
357 163 115 90 28
BssHII G'CGCG_C
Cuts at: 0 168 746 753
Size: 168 578 7
Fragments arranged by size:
578 168 7
BstYI r'GATC_y
Cuts at: 0 620 680 753
Size: 620 60 73
Fragments arranged by size:
620 73 60
EaeI y'GGCC_r
Cuts at: 0 120 318 723 753
Size: 120 198 405 30
Fragments arranged by size:
405 198 120 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 218 753
Size: 218 535
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 753
Size: 550 203
EcoO109I rG'GnC_Cy
Cuts at: 0 207 215 299 300 715 716 739 753
Size: 207 8 84 1 415 1 23 14
Fragments arranged by size:
415 207 84 23 14 8 1 1
FseI GG_CCGG'CC
Cuts at: 0 729 753
Size: 729 24
FspI TGC'GCA
Cuts at: 0 436 753
Size: 436 317
HaeII r_GCGC'y
Cuts at: 0 192 327 492 570 612 753
Size: 192 135 165 78 42 141
Fragments arranged by size:
192 165 141 135 78 42
HincII GTy'rAC
Cuts at: 0 39 753
Size: 39 714
KasI G'GCGC_C
Cuts at: 0 566 753
Size: 566 187
NaeI GCC'GGC
Cuts at: 0 117 474 727 753
Size: 117 357 253 26
Fragments arranged by size:
357 253 117 26
NarI GG'CG_CC
Cuts at: 0 567 753
Size: 567 186
NgoMIV G'CCGG_C
Cuts at: 0 115 472 725 753
Size: 115 357 253 28
Fragments arranged by size:
357 253 115 28
PasI CC'CwG_GG
Cuts at: 0 211 753
Size: 211 542
PfoI T'CCnGG_A
Cuts at: 0 308 753
Size: 308 445
PmlI CAC'GTG
Cuts at: 0 447 753
Size: 447 306
PpuMI rG'GwC_Cy
Cuts at: 0 207 753
Size: 207 546
PspOMI G'GGCC_C
Cuts at: 0 214 299 715 753
Size: 214 85 416 38
Fragments arranged by size:
416 214 85 38
RsrII CG'GwC_CG
Cuts at: 0 497 753
Size: 497 256
SalI G'TCGA_C
Cuts at: 0 37 753
Size: 37 716
SanDI GG'GwC_CC
Cuts at: 0 207 753
Size: 207 546
SfoI GGC'GCC
Cuts at: 0 568 753
Size: 568 185
SgrAI Cr'CCGG_yG
Cuts at: 0 115 472 753
Size: 115 357 281
Fragments arranged by size:
357 281 115
SmaI CCC'GGG
Cuts at: 0 105 753
Size: 105 648
SspI AAT'ATT
Cuts at: 0 67 753
Size: 67 686
XmaI C'CCGG_G
Cuts at: 0 103 753
Size: 103 650
Enzymes that do cut:
AccI AfeI ApaI ApaLI ApoI AvaI BanI BanII
BbeI BclI BglII BlpI Bme1580I BpmI BsaAI BsaBI
BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssHII BstYI EaeI EciI Eco57MI EcoO109I FseI
FspI HaeII HincII KasI NaeI NarI NgoMIV PasI
PfoI PmlI PpuMI PspOMI RsrII SalI SanDI SfoI
SgrAI SmaI SspI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI
BglI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI
FspAI Hin4I HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII SacI SacII
SapI SbfI ScaI SexAI SfcI SfiI SgfI SmlI
SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI