Restriction map for
pFN21AB8900
(Linear) (Six-base) MAPSORT of: KIBB8900.seq Check: 6994 from: 1 to: 699
KIBB8900.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 335 699
Size: 335 364
AflIII A'CryG_T
Cuts at: 0 161 699
Size: 161 538
AvaI C'yCGr_G
Cuts at: 0 121 378 699
Size: 121 257 321
Fragments arranged by size:
321 257 121
AvrII C'CTAG_G
Cuts at: 0 589 699
Size: 589 110
BanI G'GyrC_C
Cuts at: 0 382 673 699
Size: 382 291 26
Fragments arranged by size:
382 291 26
BanII G_rGCy'C
Cuts at: 0 112 181 379 699
Size: 112 69 198 320
Fragments arranged by size:
320 198 112 69
BbeI G_GCGC'C
Cuts at: 0 386 699
Size: 386 313
BglII A'GATC_T
Cuts at: 0 233 699
Size: 233 466
BlpI GC'TnA_GC
Cuts at: 0 185 699
Size: 185 514
Bme1580I G_kGCm'C
Cuts at: 0 678 699
Size: 678 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 200 699
Size: 200 499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 61 157 580 699
Size: 54 7 96 423 119
Fragments arranged by size:
423 119 96 54 7
BsaHI Gr'CG_yC
Cuts at: 0 383 699
Size: 383 316
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 6 60 313 699
Size: 6 54 253 386
Fragments arranged by size:
386 253 54 6
BseYI C'CCAG_C
Cuts at: 0 103 496 699
Size: 103 393 203
Fragments arranged by size:
393 203 103
BsiEI CG_ry'CG
Cuts at: 0 154 699
Size: 154 545
BsiHKAI G_wGCw'C
Cuts at: 0 181 379 571 699
Size: 181 198 192 128
Fragments arranged by size:
198 192 181 128
Bsp1286I G_dGCh'C
Cuts at: 0 112 181 379 571 678 699
Size: 112 69 198 192 107 21
Fragments arranged by size:
198 192 112 107 69 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 335 524 638 699
Size: 335 189 114 61
Fragments arranged by size:
335 189 114 61
BsrBI CCG'CTC
Cuts at: 0 93 184 438 699
Size: 93 91 254 261
Fragments arranged by size:
261 254 93 91
BsrFI r'CCGG_y
Cuts at: 0 353 699
Size: 353 346
BssHII G'CGCG_C
Cuts at: 0 251 699
Size: 251 448
BstXI CCAn_nnnn'nTGG
Cuts at: 0 246 699
Size: 246 453
BstYI r'GATC_y
Cuts at: 0 233 699
Size: 233 466
BtgI C'CryG_G
Cuts at: 0 56 113 699
Size: 56 57 586
Fragments arranged by size:
586 57 56
BtsI GCAGTG_nn'
Cuts at: 0 262 540 699
Size: 262 278 159
Fragments arranged by size:
278 262 159
EaeI y'GGCC_r
Cuts at: 0 151 422 699
Size: 151 271 277
Fragments arranged by size:
277 271 151
EagI C'GGCC_G
Cuts at: 0 151 699
Size: 151 548
EarI CTCTTCn'nnn_
Cuts at: 0 405 540 699
Size: 405 135 159
Fragments arranged by size:
405 159 135
EcoICRI GAG'CTC
Cuts at: 0 179 377 699
Size: 179 198 322
Fragments arranged by size:
322 198 179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 61 157 580 699
Size: 54 7 96 423 119
Fragments arranged by size:
423 119 96 54 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 139 699
Size: 139 560
EcoO109I rG'GnC_Cy
Cuts at: 0 82 699
Size: 82 617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 306 699
Size: 306 393
HaeII r_GCGC'y
Cuts at: 0 7 104 192 246 386 699
Size: 7 97 88 54 140 313
Fragments arranged by size:
313 140 97 88 54 7
KasI G'GCGC_C
Cuts at: 0 382 699
Size: 382 317
MluI A'CGCG_T
Cuts at: 0 161 699
Size: 161 538
MscI TGG'CCA
Cuts at: 0 424 699
Size: 424 275
MspA1I CmG'CkG
Cuts at: 0 115 398 428 699
Size: 115 283 30 271
Fragments arranged by size:
283 271 115 30
NarI GG'CG_CC
Cuts at: 0 383 699
Size: 383 316
PpuMI rG'GwC_Cy
Cuts at: 0 82 699
Size: 82 617
SacI G_AGCT'C
Cuts at: 0 181 379 699
Size: 181 198 320
Fragments arranged by size:
320 198 181
SacII CC_GC'GG
Cuts at: 0 116 699
Size: 116 583
SanDI GG'GwC_CC
Cuts at: 0 82 699
Size: 82 617
SapI GCTCTTCn'nnn_
Cuts at: 0 540 699
Size: 540 159
SfoI GGC'GCC
Cuts at: 0 384 699
Size: 384 315
SmaI CCC'GGG
Cuts at: 0 123 699
Size: 123 576
StuI AGG'CCT
Cuts at: 0 31 699
Size: 31 668
StyI C'CwwG_G
Cuts at: 0 589 699
Size: 589 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 478 699
Size: 478 221
XmaI C'CCGG_G
Cuts at: 0 121 699
Size: 121 578
XmnI GAAnn'nnTTC
Cuts at: 0 163 699
Size: 163 536
Enzymes that do cut:
AarI AflIII AvaI AvrII BanI BanII BbeI BglII
BlpI Bme1580I BplI BpmI BsaHI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstXI BstYI
BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII KasI MluI MscI MspA1I NarI
PpuMI SacI SacII SanDI SapI SfoI SmaI StuI
StyI TaqII XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BmgBI BmrI BmtI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EciI EcoRI EcoRV FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MmeI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SalI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI Tth111I XbaI XcmI XhoI ZraI