Restriction map for
pFN21AB8893
(Linear) (Six-base) MAPSORT of: KIBB8893.seq Check: 5630 from: 1 to: 651
KIBB8893.orf
With 182 enzymes: SgfI *
January 29, 2014 11:43 ..
AccI GT'mk_AC
Cuts at: 0 218 651
Size: 218 433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 651
Size: 647 4
AlwNI CAG_nnn'CTG
Cuts at: 0 403 447 651
Size: 403 44 204
Fragments arranged by size:
403 204 44
ApaI G_GGCC'C
Cuts at: 0 601 651
Size: 601 50
ApoI r'AATT_y
Cuts at: 0 463 651
Size: 463 188
AvaI C'yCGr_G
Cuts at: 0 120 238 600 651
Size: 120 118 362 51
Fragments arranged by size:
362 120 118 51
BanI G'GyrC_C
Cuts at: 0 325 651
Size: 325 326
BanII G_rGCy'C
Cuts at: 0 239 601 651
Size: 239 362 50
Fragments arranged by size:
362 239 50
BbeI G_GCGC'C
Cuts at: 0 329 651
Size: 329 322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 489 523 651
Size: 489 34 128
Fragments arranged by size:
489 128 34
BglI GCCn_nnn'nGGC
Cuts at: 0 319 651
Size: 319 332
Bme1580I G_kGCm'C
Cuts at: 0 601 651
Size: 601 50
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 475 651
Size: 469 6 176
Fragments arranged by size:
469 176 6
Bpu10I CC'TnA_GC
Cuts at: 0 396 651
Size: 396 255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 651
Size: 33 618
BsaHI Gr'CG_yC
Cuts at: 0 215 326 651
Size: 215 111 325
Fragments arranged by size:
325 215 111
BsaWI w'CCGG_w
Cuts at: 0 67 371 651
Size: 67 304 280
Fragments arranged by size:
304 280 67
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 649 651
Size: 649 2
BseYI C'CCAG_C
Cuts at: 0 113 651
Size: 113 538
BsiEI CG_ry'CG
Cuts at: 0 212 227 359 651
Size: 212 15 132 292
Fragments arranged by size:
292 212 132 15
BsmI GAATG_Cn'
Cuts at: 0 27 651
Size: 27 624
Bsp1286I G_dGCh'C
Cuts at: 0 239 601 651
Size: 239 362 50
Fragments arranged by size:
362 239 50
BspEI T'CCGG_A
Cuts at: 0 371 651
Size: 371 280
BsrBI CCG'CTC
Cuts at: 0 133 651
Size: 133 518
BsrFI r'CCGG_y
Cuts at: 0 211 226 560 610 651
Size: 211 15 334 50 41
Fragments arranged by size:
334 211 50 41 15
BssHII G'CGCG_C
Cuts at: 0 266 651
Size: 266 385
BstYI r'GATC_y
Cuts at: 0 374 651
Size: 374 277
BtgI C'CryG_G
Cuts at: 0 593 651
Size: 593 58
BtsI GCAGTG_nn'
Cuts at: 0 261 651
Size: 261 390
EaeI y'GGCC_r
Cuts at: 0 209 224 356 612 651
Size: 209 15 132 256 39
Fragments arranged by size:
256 209 132 39 15
EagI C'GGCC_G
Cuts at: 0 209 224 356 651
Size: 209 15 132 295
Fragments arranged by size:
295 209 132 15
EarI CTCTTCn'nnn_
Cuts at: 0 635 641 651
Size: 635 6 10
Fragments arranged by size:
635 10 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 300 651
Size: 300 351
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 475 647 651
Size: 469 6 172 4
Fragments arranged by size:
469 172 6 4
HaeII r_GCGC'y
Cuts at: 0 120 146 329 651
Size: 120 26 183 322
Fragments arranged by size:
322 183 120 26
KasI G'GCGC_C
Cuts at: 0 325 651
Size: 325 326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 651
Size: 131 520
MspA1I CmG'CkG
Cuts at: 0 595 651
Size: 595 56
NaeI GCC'GGC
Cuts at: 0 213 228 651
Size: 213 15 423
Fragments arranged by size:
423 213 15
NarI GG'CG_CC
Cuts at: 0 326 651
Size: 326 325
NgoMIV G'CCGG_C
Cuts at: 0 211 226 651
Size: 211 15 425
Fragments arranged by size:
425 211 15
NspI r_CATG'y
Cuts at: 0 261 651
Size: 261 390
PflMI CCAn_nnn'nTGG
Cuts at: 0 447 651
Size: 447 204
PspOMI G'GGCC_C
Cuts at: 0 597 651
Size: 597 54
PspXI vC'TCGA_Gb
Cuts at: 0 238 651
Size: 238 413
SacII CC_GC'GG
Cuts at: 0 596 651
Size: 596 55
SfoI GGC'GCC
Cuts at: 0 327 651
Size: 327 324
SmaI CCC'GGG
Cuts at: 0 122 602 651
Size: 122 480 49
Fragments arranged by size:
480 122 49
SmlI C'TyrA_G
Cuts at: 0 48 238 651
Size: 48 190 413
Fragments arranged by size:
413 190 48
SrfI GCCC'GGGC
Cuts at: 0 122 651
Size: 122 529
StyI C'CwwG_G
Cuts at: 0 314 329 651
Size: 314 15 322
Fragments arranged by size:
322 314 15
XhoI C'TCGA_G
Cuts at: 0 238 651
Size: 238 413
XmaI C'CCGG_G
Cuts at: 0 120 600 651
Size: 120 480 51
Fragments arranged by size:
480 120 51
Enzymes that do cut:
AccI AcuI AlwNI ApaI ApoI AvaI BanI BanII
BbeI BcgI BglI Bme1580I BpmI Bpu10I BpuEI BsaHI
BsaWI BseRI BseYI BsiEI BsmI Bsp1286I BspEI BsrBI
BsrFI BssHII BstYI BtgI BtsI EaeI EagI EarI
EciI Eco57MI HaeII KasI MmeI MspA1I NaeI NarI
NgoMIV NspI PflMI PspOMI PspXI SacII SfoI SmaI
SmlI SrfI StyI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BsaI BsaAI
BsaBI BsaXI BsgI BsiHKAI BsiWI BsmBI BspHI BspMI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI
MslI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SnaBI SpeI SphI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmnI ZraI