(Linear) (Six-base) MAPSORT of: KIBB8826.seq  Check: 5903  from: 1  to: 1245



KIBB8826.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 15:44  ..

AccI GT'mk_AC

Cuts at:      0     292    1245 
   Size:        292     953
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     950    1245 
   Size:        950     295
 
AgeI A'CCGG_T

Cuts at:      0     485    1245 
   Size:        485     760
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     436    1245 
   Size:        436     809
 
AvaI C'yCGr_G

Cuts at:      0     184    1245 
   Size:        184    1061
 
BanI G'GyrC_C

Cuts at:      0      60     893    1245 
   Size:         60     833     352

  Fragments arranged by size: 

                 833     352      60
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     342     376    1245 
   Size:        342      34     869

  Fragments arranged by size: 

                 869     342      34
 
BglII A'GATC_T

Cuts at:      0     455    1245 
   Size:        455     790
 
BlpI GC'TnA_GC

Cuts at:      0     884    1245 
   Size:        884     361
 
Bme1580I G_kGCm'C

Cuts at:      0      65    1245 
   Size:         65    1180
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     624    1245 
   Size:        624     621
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     428    1245 
   Size:        355      73     817

  Fragments arranged by size: 

                 817     355      73
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     318    1245 
   Size:        318     927
 
BsaWI w'CCGG_w

Cuts at:      0     485    1245 
   Size:        485     760
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148     457     636     795    1245 
   Size:        148     309     179     159     450

  Fragments arranged by size: 

                 450     309     179     159     148
 
BseYI C'CCAG_C

Cuts at:      0     396    1245 
   Size:        396     849
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66    1245 
   Size:         66    1179
 
BsiHKAI G_wGCw'C

Cuts at:      0     140     596    1245 
   Size:        140     456     649

  Fragments arranged by size: 

                 649     456     140
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     140     596    1245 
   Size:         65      75     456     649

  Fragments arranged by size: 

                 649     456      75      65
 
BsrDI GCAATG_nn'

Cuts at:      0    1071    1245 
   Size:       1071     174
 
BsrFI r'CCGG_y

Cuts at:      0     485    1245 
   Size:        485     760
 
BstYI r'GATC_y

Cuts at:      0     455     563    1245 
   Size:        455     108     682

  Fragments arranged by size: 

                 682     455     108
 
BtgI C'CryG_G

Cuts at:      0     317    1136    1245 
   Size:        317     819     109

  Fragments arranged by size: 

                 819     317     109
 
BtsI GCAGTG_nn'

Cuts at:      0     659    1245 
   Size:        659     586
 
DraIII CAC_nnn'GTG

Cuts at:      0     174    1245 
   Size:        174    1071
 
EaeI y'GGCC_r

Cuts at:      0     194     768    1097    1139    1245 
   Size:        194     574     329      42     106

  Fragments arranged by size: 

                 574     329     194     106      42
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     428     950    1245 
   Size:        355      73     522     295

  Fragments arranged by size: 

                 522     355     295      73
 
EcoRV GAT'ATC

Cuts at:      0     953    1245 
   Size:        953     292
 
HindIII A'AGCT_T

Cuts at:      0    1201    1245 
   Size:       1201      44
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     635    1245 
   Size:        124     511     610

  Fragments arranged by size: 

                 610     511     124
 
MscI TGG'CCA

Cuts at:      0     196    1099    1141    1245 
   Size:        196     903      42     104

  Fragments arranged by size: 

                 903     196     104      42
 
MslI CAynn'nnrTG

Cuts at:      0     899    1245 
   Size:        899     346
 
MspA1I CmG'CkG

Cuts at:      0      80    1245 
   Size:         80    1165
 
NcoI C'CATG_G

Cuts at:      0     317    1136    1245 
   Size:        317     819     109

  Fragments arranged by size: 

                 819     317     109
 
NspI r_CATG'y

Cuts at:      0     326    1041    1245 
   Size:        326     715     204

  Fragments arranged by size: 

                 715     326     204
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     684    1245 
   Size:        684     561
 
PsiI TTA'TAA

Cuts at:      0     998    1245 
   Size:        998     247
 
PvuII CAG'CTG

Cuts at:      0      80    1245 
   Size:         80    1165
 
SmaI CCC'GGG

Cuts at:      0     186    1245 
   Size:        186    1059
 
StyI C'CwwG_G

Cuts at:      0     317    1136    1190    1245 
   Size:        317     819      54      55

  Fragments arranged by size: 

                 819     317      55      54
 
Tth111I GACn'n_nGTC

Cuts at:      0     288    1245 
   Size:        288     957
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     306     379    1245 
   Size:        306      73     866

  Fragments arranged by size: 

                 866     306      73
 
XmaI C'CCGG_G

Cuts at:      0     184    1245 
   Size:        184    1061
 

 Enzymes that do cut:

     AccI     AcuI     AgeI     AlfI     AvaI     BanI     BcgI    BglII
     BlpI Bme1580I     BmrI     BpmI     BsaI    BsaWI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BsrDI    BsrFI    BstYI     BtgI     BtsI
   DraIII     EaeI  Eco57MI    EcoRV  HindIII     MmeI     MscI     MslI
   MspA1I     NcoI     NspI    PflMI     PsiI    PvuII     SmaI     StyI
  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
    BciVI     BclI    BfrBI     BglI    BmgBI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI     XbaI     XhoI     XmnI
     ZraI