Restriction map for
pFN21AB8802
(Linear) (Six-base) MAPSORT of: KIBB8802.seq Check: 3403 from: 1 to: 1038
KIBB8802.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AccI GT'mk_AC
Cuts at: 0 234 1038
Size: 234 804
AleI CACnn'nnGTG
Cuts at: 0 388 1038
Size: 388 650
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 364 396 1038
Size: 364 32 642
Fragments arranged by size:
642 364 32
AlwNI CAG_nnn'CTG
Cuts at: 0 624 1038
Size: 624 414
ApaI G_GGCC'C
Cuts at: 0 423 777 1038
Size: 423 354 261
Fragments arranged by size:
423 354 261
ApoI r'AATT_y
Cuts at: 0 859 1038
Size: 859 179
AvaI C'yCGr_G
Cuts at: 0 386 744 1038
Size: 386 358 294
Fragments arranged by size:
386 358 294
BamHI G'GATC_C
Cuts at: 0 819 1014 1038
Size: 819 195 24
Fragments arranged by size:
819 195 24
BanI G'GyrC_C
Cuts at: 0 738 1038
Size: 738 300
BanII G_rGCy'C
Cuts at: 0 275 423 777 1038
Size: 275 148 354 261
Fragments arranged by size:
354 275 261 148
BbsI GAAGACnn'nnnn_
Cuts at: 0 139 1038
Size: 139 899
BbvCI CC'TCA_GC
Cuts at: 0 790 1038
Size: 790 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 84 118 1038
Size: 84 34 920
Fragments arranged by size:
920 84 34
BglI GCCn_nnn'nGGC
Cuts at: 0 634 1038
Size: 634 404
Bme1580I G_kGCm'C
Cuts at: 0 423 743 777 1038
Size: 423 320 34 261
Fragments arranged by size:
423 320 261 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 249 1038
Size: 249 789
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 289 1038
Size: 289 749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 568 1038
Size: 475 93 470
Fragments arranged by size:
475 470 93
Bpu10I CC'TnA_GC
Cuts at: 0 790 1038
Size: 790 248
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 55 364 394 1038
Size: 25 30 309 30 644
Fragments arranged by size:
644 309 30 30 25
BseYI C'CCAG_C
Cuts at: 0 81 145 703 1038
Size: 81 64 558 335
Fragments arranged by size:
558 335 81 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 763 1038
Size: 393 370 275
Fragments arranged by size:
393 370 275
BsiEI CG_ry'CG
Cuts at: 0 485 957 1038
Size: 485 472 81
Fragments arranged by size:
485 472 81
BsmBI CGTCTCn'nnnn_
Cuts at: 0 472 492 1038
Size: 472 20 546
Fragments arranged by size:
546 472 20
Bsp1286I G_dGCh'C
Cuts at: 0 275 423 743 777 1038
Size: 275 148 320 34 261
Fragments arranged by size:
320 275 261 148 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 707 1038
Size: 707 331
BsrDI GCAATG_nn'
Cuts at: 0 102 1015 1038
Size: 102 913 23
Fragments arranged by size:
913 102 23
BsrGI T'GTAC_A
Cuts at: 0 2 1038
Size: 2 1036
BstAPI GCAn_nnn'nTGC
Cuts at: 0 407 965 1038
Size: 407 558 73
Fragments arranged by size:
558 407 73
BstBI TT'CG_AA
Cuts at: 0 946 1038
Size: 946 92
BstXI CCAn_nnnn'nTGG
Cuts at: 0 390 1003 1038
Size: 390 613 35
Fragments arranged by size:
613 390 35
BstYI r'GATC_y
Cuts at: 0 819 1014 1038
Size: 819 195 24
Fragments arranged by size:
819 195 24
Bsu36I CC'TnA_GG
Cuts at: 0 607 1038
Size: 607 431
BtgI C'CryG_G
Cuts at: 0 173 768 1038
Size: 173 595 270
Fragments arranged by size:
595 270 173
DraIII CAC_nnn'GTG
Cuts at: 0 369 416 1038
Size: 369 47 622
Fragments arranged by size:
622 369 47
EaeI y'GGCC_r
Cuts at: 0 170 284 482 515 626 1038
Size: 170 114 198 33 111 412
Fragments arranged by size:
412 198 170 114 111 33
EagI C'GGCC_G
Cuts at: 0 482 1038
Size: 482 556
EarI CTCTTCn'nnn_
Cuts at: 0 280 926 1038
Size: 280 646 112
Fragments arranged by size:
646 280 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 627 1038
Size: 627 411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 568 1038
Size: 475 93 470
Fragments arranged by size:
475 470 93
EcoO109I rG'GnC_Cy
Cuts at: 0 420 773 774 1038
Size: 420 353 1 264
Fragments arranged by size:
420 353 264 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 1038
Size: 25 32 981
Fragments arranged by size:
981 32 25
MfeI C'AATT_G
Cuts at: 0 677 1038
Size: 677 361
MscI TGG'CCA
Cuts at: 0 286 628 1038
Size: 286 342 410
Fragments arranged by size:
410 342 286
MslI CAynn'nnrTG
Cuts at: 0 388 667 670 802 973 1038
Size: 388 279 3 132 171 65
Fragments arranged by size:
388 279 171 132 65 3
MspA1I CmG'CkG
Cuts at: 0 60 546 624 1038
Size: 60 486 78 414
Fragments arranged by size:
486 414 78 60
NcoI C'CATG_G
Cuts at: 0 768 1038
Size: 768 270
NspI r_CATG'y
Cuts at: 0 695 816 1038
Size: 695 121 222
Fragments arranged by size:
695 222 121
PflMI CCAn_nnn'nTGG
Cuts at: 0 524 1038
Size: 524 514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 364 396 1038
Size: 364 32 642
Fragments arranged by size:
642 364 32
PshAI GACnn'nnGTC
Cuts at: 0 484 1038
Size: 484 554
PspOMI G'GGCC_C
Cuts at: 0 419 773 1038
Size: 419 354 265
Fragments arranged by size:
419 354 265
PspXI vC'TCGA_Gb
Cuts at: 0 744 1038
Size: 744 294
PstI C_TGCA'G
Cuts at: 0 886 1038
Size: 886 152
PvuII CAG'CTG
Cuts at: 0 546 624 1038
Size: 546 78 414
Fragments arranged by size:
546 414 78
SapI GCTCTTCn'nnn_
Cuts at: 0 280 1038
Size: 280 758
SfcI C'TryA_G
Cuts at: 0 882 1038
Size: 882 156
SmaI CCC'GGG
Cuts at: 0 388 1038
Size: 388 650
SmlI C'TyrA_G
Cuts at: 0 744 1038
Size: 744 294
SphI G_CATG'C
Cuts at: 0 816 1038
Size: 816 222
StyI C'CwwG_G
Cuts at: 0 768 1038
Size: 768 270
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 943 1038
Size: 943 95
TatI w'GTAC_w
Cuts at: 0 2 1038
Size: 2 1036
XhoI C'TCGA_G
Cuts at: 0 744 1038
Size: 744 294
XmaI C'CCGG_G
Cuts at: 0 386 1038
Size: 386 652
Enzymes that do cut:
AccI AleI AloI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BcgI BglI Bme1580I BmrI
BplI BpmI Bpu10I BsaXI BseYI BsgI BsiEI BsmBI
Bsp1286I BspMI BsrDI BsrGI BstAPI BstBI BstXI BstYI
Bsu36I BtgI DraIII EaeI EagI EarI EciI Eco57MI
EcoO109I Hin4I MfeI MscI MslI MspA1I NcoI NspI
PflMI PpiI PshAI PspOMI PspXI PstI PvuII SapI
SfcI SmaI SmlI SphI StyI TaqII TatI XhoI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BsiHKAI BsiWI BsmI BspEI BspHI BsrBI BsrFI BssHII
BssSI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI
DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpuMI
PsiI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XmnI ZraI