Restriction map for
pFN21AB8549
(Linear) (Six-base) MAPSORT of: KIBB8549.seq Check: 7604 from: 1 to: 2970
KIBB8549.orf
With 182 enzymes: SgfI *
July 3, 2012 16:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1203 1385 1826 2282 2970
Size: 1203 182 441 456 688
Fragments arranged by size:
1203 688 456 441 182
Acc65I G'GTAC_C
Cuts at: 0 449 2970
Size: 449 2521
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 737 913 1765 1783 2257 2449 2554
Size: 451 286 176 852 18 474 192 105
Cuts at: 2554 2693 2970
Size: 139 277
Fragments arranged by size:
852 474 451 286 277 192 176 139
105 18
AflIII A'CryG_T
Cuts at: 0 391 2970
Size: 391 2579
AlwNI CAG_nnn'CTG
Cuts at: 0 1266 1809 1848 1884 2625 2970
Size: 1266 543 39 36 741 345
Fragments arranged by size:
1266 741 543 345 39 36
ApaI G_GGCC'C
Cuts at: 0 412 1337 2025 2364 2718 2970
Size: 412 925 688 339 354 252
Fragments arranged by size:
925 688 412 354 339 252
ApaLI G'TGCA_C
Cuts at: 0 1216 2970
Size: 1216 1754
AvaI C'yCGr_G
Cuts at: 0 2882 2970
Size: 2882 88
BamHI G'GATC_C
Cuts at: 0 635 2970
Size: 635 2335
BanI G'GyrC_C
Cuts at: 0 244 449 2261 2543 2970
Size: 244 205 1812 282 427
Fragments arranged by size:
1812 427 282 244 205
BanII G_rGCy'C
Cuts at: 0 412 597 777 1325 1337 1502 1598 2025
Size: 412 185 180 548 12 165 96 427
Cuts at: 2025 2364 2408 2705 2718 2970
Size: 339 44 297 13 252
Fragments arranged by size:
548 427 412 339 297 252 185 180
165 96 44 13 12
BbeI G_GCGC'C
Cuts at: 0 2265 2970
Size: 2265 705
BbvCI CC'TCA_GC
Cuts at: 0 2123 2970
Size: 2123 847
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1658 1692 2845 2879 2970
Size: 1658 34 1153 34 91
Fragments arranged by size:
1658 1153 91 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 162 2970
Size: 162 2808
BglI GCCn_nnn'nGGC
Cuts at: 0 1951 2173 2780 2970
Size: 1951 222 607 190
Fragments arranged by size:
1951 607 222 190
BglII A'GATC_T
Cuts at: 0 720 2970
Size: 720 2250
BlpI GC'TnA_GC
Cuts at: 0 1370 1613 1735 2970
Size: 1370 243 122 1235
Fragments arranged by size:
1370 1235 243 122
Bme1580I G_kGCm'C
Cuts at: 0 412 1083 1220 1337 2025 2364 2718 2970
Size: 412 671 137 117 688 339 354 252
Fragments arranged by size:
688 671 412 354 339 252 137 117
BmgBI CAC'GTC
Cuts at: 0 1689 2970
Size: 1689 1281
BmrI ACTGGGnnnn_n'
Cuts at: 0 2019 2970
Size: 2019 951
BmtI G_CTAG'C
Cuts at: 0 463 2970
Size: 463 2507
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 865 2970
Size: 865 2105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 462 877 1288 1411 1498 1504 1558
Size: 460 2 415 411 123 87 6 54
Cuts at: 1558 2173 2495 2632 2970
Size: 615 322 137 338
Fragments arranged by size:
615 460 415 411 338 322 137 123
87 54 6 2
Bpu10I CC'TnA_GC
Cuts at: 0 1928 2123 2970
Size: 1928 195 847
Fragments arranged by size:
1928 847 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1461 1582 2970
Size: 1461 121 1388
Fragments arranged by size:
1461 1388 121
BsaI GGTCTCn'nnnn_
Cuts at: 0 1534 1793 2344 2970
Size: 1534 259 551 626
Fragments arranged by size:
1534 626 551 259
BsaAI yAC'GTr
Cuts at: 0 2227 2970
Size: 2227 743
BsaHI Gr'CG_yC
Cuts at: 0 845 2262 2970
Size: 845 1417 708
Fragments arranged by size:
1417 845 708
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 433 463 2970
Size: 433 30 2507
Fragments arranged by size:
2507 433 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1087 1246 1480 2089 2092 2095 2104 2107
Size: 1087 159 234 609 3 3 9 3
Cuts at: 2107 2110 2113 2116 2119 2221 2224 2572 2605
Size: 3 3 3 3 102 3 348 33
Cuts at: 2605 2970
Size: 365
Fragments arranged by size:
1087 609 365 348 234 159 102 33
9 3 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 1326 1701 1713 1895 2000 2313 2718 2970
Size: 1326 375 12 182 105 313 405 252
Fragments arranged by size:
1326 405 375 313 252 182 105 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2825 2970
Size: 2825 145
BsiEI CG_ry'CG
Cuts at: 0 1496 1557 2649 2970
Size: 1496 61 1092 321
Fragments arranged by size:
1496 1092 321 61
BsiHKAI G_wGCw'C
Cuts at: 0 1220 1376 1502 1598 2408 2970
Size: 1220 156 126 96 810 562
Fragments arranged by size:
1220 810 562 156 126 96
BsmI GAATG_Cn'
Cuts at: 0 580 2915 2970
Size: 580 2335 55
Fragments arranged by size:
2335 580 55
BsmBI CGTCTCn'nnnn_
Cuts at: 0 836 1264 2970
Size: 836 428 1706
Fragments arranged by size:
1706 836 428
Bsp1286I G_dGCh'C
Cuts at: 0 412 597 777 1083 1220 1325 1337 1376
Size: 412 185 180 306 137 105 12 39
Cuts at: 1376 1502 1598 2025 2364 2408 2705 2718 2970
Size: 126 96 427 339 44 297 13 252
Fragments arranged by size:
427 412 339 306 297 252 185 180
137 126 105 96 44 39 13 12
BspHI T'CATG_A
Cuts at: 0 279 2970
Size: 279 2691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1095 1203 1385 1826 2282 2970
Size: 1095 108 182 441 456 688
Fragments arranged by size:
1095 688 456 441 182 108
BsrBI CCG'CTC
Cuts at: 0 771 1077 1488 1625 2541 2970
Size: 771 306 411 137 916 429
Fragments arranged by size:
916 771 429 411 306 137
BsrDI GCAATG_nn'
Cuts at: 0 888 2501 2970
Size: 888 1613 469
Fragments arranged by size:
1613 888 469
BsrFI r'CCGG_y
Cuts at: 0 446 1025 2258 2779 2970
Size: 446 579 1233 521 191
Fragments arranged by size:
1233 579 521 446 191
BssHII G'CGCG_C
Cuts at: 0 1522 2970
Size: 1522 1448
BssSI C'ACGA_G
Cuts at: 0 1900 2970
Size: 1900 1070
BstAPI GCAn_nnn'nTGC
Cuts at: 0 421 1194 2250 2853 2912 2970
Size: 421 773 1056 603 59 58
Fragments arranged by size:
1056 773 603 421 59 58
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2473 2734 2970
Size: 2473 261 236
Fragments arranged by size:
2473 261 236
BstYI r'GATC_y
Cuts at: 0 435 635 720 2970
Size: 435 200 85 2250
Fragments arranged by size:
2250 435 200 85
Bsu36I CC'TnA_GG
Cuts at: 0 164 598 850 2300 2970
Size: 164 434 252 1450 670
Fragments arranged by size:
1450 670 434 252 164
BtgI C'CryG_G
Cuts at: 0 1067 2168 2375 2970
Size: 1067 1101 207 595
Fragments arranged by size:
1101 1067 595 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2440 2970
Size: 2440 530
BtsI GCAGTG_nn'
Cuts at: 0 1669 2534 2790 2837 2970
Size: 1669 865 256 47 133
Fragments arranged by size:
1669 865 256 133 47
EaeI y'GGCC_r
Cuts at: 0 29 725 1493 1832 1943 2970
Size: 29 696 768 339 111 1027
Fragments arranged by size:
1027 768 696 339 111 29
EagI C'GGCC_G
Cuts at: 0 1493 2970
Size: 1493 1477
EarI CTCTTCn'nnn_
Cuts at: 0 911 1759 2970
Size: 911 848 1211
Fragments arranged by size:
1211 911 848
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1531 2970
Size: 1531 1439
EcoICRI GAG'CTC
Cuts at: 0 1500 1596 2406 2970
Size: 1500 96 810 564
Fragments arranged by size:
1500 810 564 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 460 462 737 877 913 1288 1411
Size: 451 9 2 275 140 36 375 123
Cuts at: 1411 1498 1504 1558 1765 1783 2173 2257 2449
Size: 87 6 54 207 18 390 84 192
Cuts at: 2449 2495 2554 2632 2693 2970
Size: 46 59 78 61 277
Fragments arranged by size:
451 390 375 277 275 207 192 140
123 87 84 78 61 59 54 46
36 18 9 6 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1102 1381 2051 2798 2970
Size: 1102 279 670 747 172
Fragments arranged by size:
1102 747 670 279 172
EcoO109I rG'GnC_Cy
Cuts at: 0 408 409 888 930 1333 1334 2148 2361
Size: 408 1 479 42 403 1 814 213
Cuts at: 2361 2714 2715 2970
Size: 353 1 255
Fragments arranged by size:
814 479 408 403 353 255 213 42
1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 940 2970
Size: 940 2030
HaeII r_GCGC'y
Cuts at: 0 1178 1986 2265 2892 2970
Size: 1178 808 279 627 78
Fragments arranged by size:
1178 808 627 279 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 42 74 2970
Size: 42 32 2896
Fragments arranged by size:
2896 42 32
HincII GTy'rAC
Cuts at: 0 830 2970
Size: 830 2140
HindIII A'AGCT_T
Cuts at: 0 922 2970
Size: 922 2048
HpaI GTT'AAC
Cuts at: 0 830 2970
Size: 830 2140
KasI G'GCGC_C
Cuts at: 0 2261 2970
Size: 2261 709
KpnI G_GTAC'C
Cuts at: 0 453 2970
Size: 453 2517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 746 1658 2970
Size: 536 210 912 1312
Fragments arranged by size:
1312 912 536 210
MscI TGG'CCA
Cuts at: 0 727 2970
Size: 727 2243
MslI CAynn'nnrTG
Cuts at: 0 259 2970
Size: 259 2711
MspA1I CmG'CkG
Cuts at: 0 1086 1608 1848 1911 2191 2235 2680 2859
Size: 1086 522 240 63 280 44 445 179
Cuts at: 2859 2970
Size: 111
Fragments arranged by size:
1086 522 445 280 240 179 111 63
44
NaeI GCC'GGC
Cuts at: 0 2260 2781 2970
Size: 2260 521 189
Fragments arranged by size:
2260 521 189
NarI GG'CG_CC
Cuts at: 0 2262 2970
Size: 2262 708
NcoI C'CATG_G
Cuts at: 0 1067 2168 2375 2970
Size: 1067 1101 207 595
Fragments arranged by size:
1101 1067 595 207
NgoMIV G'CCGG_C
Cuts at: 0 2258 2779 2970
Size: 2258 521 191
Fragments arranged by size:
2258 521 191
NheI G'CTAG_C
Cuts at: 0 459 2970
Size: 459 2511
NspI r_CATG'y
Cuts at: 0 395 691 957 2970
Size: 395 296 266 2013
Fragments arranged by size:
2013 395 296 266
PasI CC'CwG_GG
Cuts at: 0 2047 2320 2970
Size: 2047 273 650
Fragments arranged by size:
2047 650 273
PciI A'CATG_T
Cuts at: 0 391 2970
Size: 391 2579
PflMI CCAn_nnn'nTGG
Cuts at: 0 1809 1902 2970
Size: 1809 93 1068
Fragments arranged by size:
1809 1068 93
PfoI T'CCnGG_A
Cuts at: 0 1535 2141 2970
Size: 1535 606 829
Fragments arranged by size:
1535 829 606
PpuMI rG'GwC_Cy
Cuts at: 0 888 930 2148 2970
Size: 888 42 1218 822
Fragments arranged by size:
1218 888 822 42
PshAI GACnn'nnGTC
Cuts at: 0 394 2970
Size: 394 2576
PspOMI G'GGCC_C
Cuts at: 0 408 1333 2021 2360 2714 2970
Size: 408 925 688 339 354 256
Fragments arranged by size:
925 688 408 354 339 256
PstI C_TGCA'G
Cuts at: 0 740 1622 1676 1727 1790 1823 1892 1916
Size: 740 882 54 51 63 33 69 24
Cuts at: 1916 2369 2390 2621 2970
Size: 453 21 231 349
Fragments arranged by size:
882 740 453 349 231 69 63 54
51 33 24 21
PvuII CAG'CTG
Cuts at: 0 1086 1608 1848 1911 2191 2235 2680 2859
Size: 1086 522 240 63 280 44 445 179
Cuts at: 2859 2970
Size: 111
Fragments arranged by size:
1086 522 445 280 240 179 111 63
44
SacI G_AGCT'C
Cuts at: 0 1502 1598 2408 2970
Size: 1502 96 810 562
Fragments arranged by size:
1502 810 562 96
SapI GCTCTTCn'nnn_
Cuts at: 0 1759 2970
Size: 1759 1211
SbfI CC_TGCA'GG
Cuts at: 0 2369 2970
Size: 2369 601
SfcI C'TryA_G
Cuts at: 0 86 736 1618 1672 1723 1786 1819 1888
Size: 86 650 882 54 51 63 33 69
Cuts at: 1888 1912 2192 2365 2386 2617 2970
Size: 24 280 173 21 231 353
Fragments arranged by size:
882 650 353 280 231 173 86 69
63 54 51 33 24 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1951 2970
Size: 1951 1019
SfoI GGC'GCC
Cuts at: 0 2263 2970
Size: 2263 707
SmaI CCC'GGG
Cuts at: 0 2884 2970
Size: 2884 86
SmlI C'TyrA_G
Cuts at: 0 1440 1597 2970
Size: 1440 157 1373
Fragments arranged by size:
1440 1373 157
SphI G_CATG'C
Cuts at: 0 691 957 2970
Size: 691 266 2013
Fragments arranged by size:
2013 691 266
StyI C'CwwG_G
Cuts at: 0 176 1067 2168 2375 2799 2970
Size: 176 891 1101 207 424 171
Fragments arranged by size:
1101 891 424 207 176 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 189 889 2014 2970
Size: 189 700 1125 956
Fragments arranged by size:
1125 956 700 189
TatI w'GTAC_w
Cuts at: 0 2498 2561 2970
Size: 2498 63 409
Fragments arranged by size:
2498 409 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2316 2348 2970
Size: 2316 32 622
Fragments arranged by size:
2316 622 32
Tth111I GACn'n_nGTC
Cuts at: 0 292 2970
Size: 292 2678
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 364 2970
Size: 364 2606
XmaI C'CCGG_G
Cuts at: 0 2882 2970
Size: 2882 88
Enzymes that do cut:
AarI Acc65I AcuI AflIII AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI
SapI SbfI SfcI SfiI SfoI SmaI SmlI SphI
StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BbsI BclI BfrBI BsaBI BsaWI BsiWI BspEI BsrGI
BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI
EcoRI EcoRV FseI FspI FspAI MfeI MluI NdeI
NotI NruI NsiI PacI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI
SexAI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
SwaI XbaI XhoI XmnI ZraI