Restriction map for
pFN21AB8544
(Linear) (Six-base) MAPSORT of: KIBB8544.seq Check: 8601 from: 1 to: 2706
KIBB8544.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AatII G_ACGT'C
Cuts at: 0 2658 2706
Size: 2658 48
AccI GT'mk_AC
Cuts at: 0 2278 2706
Size: 2278 428
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 926 1157 1196 1666 1870 2706
Size: 305 621 231 39 470 204 836
Fragments arranged by size:
836 621 470 305 231 204 39
AflIII A'CryG_T
Cuts at: 0 2447 2449 2706
Size: 2447 2 257
Fragments arranged by size:
2447 257 2
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 776 808 2706
Size: 776 32 1898
Fragments arranged by size:
1898 776 32
AlwNI CAG_nnn'CTG
Cuts at: 0 283 2706
Size: 283 2423
ApoI r'AATT_y
Cuts at: 0 172 662 1543 1754 2388 2465 2562 2706
Size: 172 490 881 211 634 77 97 144
Fragments arranged by size:
881 634 490 211 172 144 97 77
AvaI C'yCGr_G
Cuts at: 0 9 968 2706
Size: 9 959 1738
Fragments arranged by size:
1738 959 9
AvrII C'CTAG_G
Cuts at: 0 1245 2706
Size: 1245 1461
BanII G_rGCy'C
Cuts at: 0 829 845 1307 1658 2706
Size: 829 16 462 351 1048
Fragments arranged by size:
1048 829 462 351 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 476 617 1582 2706
Size: 476 141 965 1124
Fragments arranged by size:
1124 965 476 141
BbvCI CC'TCA_GC
Cuts at: 0 196 2706
Size: 196 2510
BciVI GTATCCnnnnn_n'
Cuts at: 0 1953 2706
Size: 1953 753
BclI T'GATC_A
Cuts at: 0 432 2706
Size: 432 2274
BfrBI ATG'CAT
Cuts at: 0 1673 2706
Size: 1673 1033
BglII A'GATC_T
Cuts at: 0 2625 2706
Size: 2625 81
BlpI GC'TnA_GC
Cuts at: 0 452 2706
Size: 452 2254
Bme1580I G_kGCm'C
Cuts at: 0 1149 2447 2706
Size: 1149 1298 259
Fragments arranged by size:
1298 1149 259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 1688 2706
Size: 397 1291 1018
Fragments arranged by size:
1291 1018 397
Bpu10I CC'TnA_GC
Cuts at: 0 196 2706
Size: 196 2510
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 797 935 1584 2505 2706
Size: 797 138 649 921 201
Fragments arranged by size:
921 797 649 201 138
BsaAI yAC'GTr
Cuts at: 0 1771 2450 2706
Size: 1771 679 256
Fragments arranged by size:
1771 679 256
BsaBI GATnn'nnATC
Cuts at: 0 1131 1192 2630 2706
Size: 1131 61 1438 76
Fragments arranged by size:
1438 1131 76 61
BsaHI Gr'CG_yC
Cuts at: 0 2655 2706
Size: 2655 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1439 1469 2045 2075 2230 2260 2290 2706
Size: 1439 30 576 30 155 30 30 416
Fragments arranged by size:
1439 576 416 155 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 935 938 2066 2281 2437 2706
Size: 935 3 1128 215 156 269
Fragments arranged by size:
1128 935 269 215 156 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 1611 2706
Size: 296 1315 1095
Fragments arranged by size:
1315 1095 296
BsiHKAI G_wGCw'C
Cuts at: 0 587 845 2706
Size: 587 258 1861
Fragments arranged by size:
1861 587 258
BsmI GAATG_Cn'
Cuts at: 0 1675 2706
Size: 1675 1031
Bsp1286I G_dGCh'C
Cuts at: 0 587 829 845 1149 1307 1658 2447 2706
Size: 587 242 16 304 158 351 789 259
Fragments arranged by size:
789 587 351 304 259 242 158 16
BspHI T'CATG_A
Cuts at: 0 90 219 1824 2706
Size: 90 129 1605 882
Fragments arranged by size:
1605 882 129 90
BsrFI r'CCGG_y
Cuts at: 0 521 2706
Size: 521 2185
BsrGI T'GTAC_A
Cuts at: 0 2074 2706
Size: 2074 632
BstYI r'GATC_y
Cuts at: 0 1473 1971 2625 2706
Size: 1473 498 654 81
Fragments arranged by size:
1473 654 498 81
BtgI C'CryG_G
Cuts at: 0 1857 2706
Size: 1857 849
ClaI AT'CG_AT
Cuts at: 0 1485 2706
Size: 1485 1221
DraI TTT'AAA
Cuts at: 0 1752 2358 2706
Size: 1752 606 348
Fragments arranged by size:
1752 606 348
DraIII CAC_nnn'GTG
Cuts at: 0 591 2706
Size: 591 2115
EaeI y'GGCC_r
Cuts at: 0 704 2706
Size: 704 2002
EarI CTCTTCn'nnn_
Cuts at: 0 2210 2258 2706
Size: 2210 48 448
Fragments arranged by size:
2210 448 48
EcoICRI GAG'CTC
Cuts at: 0 843 2706
Size: 843 1863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 397 926 1157 1196 1666 1688 1870
Size: 305 92 529 231 39 470 22 182
Cuts at: 1870 2706
Size: 836
Fragments arranged by size:
836 529 470 305 231 182 92 39
22
EcoO109I rG'GnC_Cy
Cuts at: 0 63 817 2706
Size: 63 754 1889
Fragments arranged by size:
1889 754 63
EcoRI G'AATT_C
Cuts at: 0 172 2706
Size: 172 2534
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1547 1576 2706
Size: 1547 29 1130
Fragments arranged by size:
1547 1130 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 730 762 933 965 1956 1988 2209 2230
Size: 730 32 171 32 991 32 221 21
Cuts at: 2230 2241 2260 2262 2292 2706
Size: 11 19 2 30 414
Fragments arranged by size:
991 730 414 221 171 32 32 32
30 21 19 11 2
HincII GTy'rAC
Cuts at: 0 519 2706
Size: 519 2187
HindIII A'AGCT_T
Cuts at: 0 397 1385 2706
Size: 397 988 1321
Fragments arranged by size:
1321 988 397
HpaI GTT'AAC
Cuts at: 0 519 2706
Size: 519 2187
MscI TGG'CCA
Cuts at: 0 706 2706
Size: 706 2000
MslI CAynn'nnrTG
Cuts at: 0 1668 2303 2706
Size: 1668 635 403
Fragments arranged by size:
1668 635 403
MspA1I CmG'CkG
Cuts at: 0 20 195 451 2706
Size: 20 175 256 2255
Fragments arranged by size:
2255 256 175 20
NcoI C'CATG_G
Cuts at: 0 1857 2706
Size: 1857 849
NdeI CA'TA_TG
Cuts at: 0 293 2706
Size: 293 2413
NsiI A_TGCA'T
Cuts at: 0 1675 2706
Size: 1675 1031
PasI CC'CwG_GG
Cuts at: 0 1648 2706
Size: 1648 1058
PflMI CCAn_nnn'nTGG
Cuts at: 0 855 2706
Size: 855 1851
PfoI T'CCnGG_A
Cuts at: 0 1974 2706
Size: 1974 732
PmlI CAC'GTG
Cuts at: 0 1771 2450 2706
Size: 1771 679 256
Fragments arranged by size:
1771 679 256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1803 1835 2706
Size: 1803 32 871
Fragments arranged by size:
1803 871 32
PpuMI rG'GwC_Cy
Cuts at: 0 63 817 2706
Size: 63 754 1889
Fragments arranged by size:
1889 754 63
PshAI GACnn'nnGTC
Cuts at: 0 689 2706
Size: 689 2017
PsiI TTA'TAA
Cuts at: 0 1222 2706
Size: 1222 1484
PspXI vC'TCGA_Gb
Cuts at: 0 968 2706
Size: 968 1738
PstI C_TGCA'G
Cuts at: 0 42 2706
Size: 42 2664
PvuII CAG'CTG
Cuts at: 0 20 195 451 2706
Size: 20 175 256 2255
Fragments arranged by size:
2255 256 175 20
SacI G_AGCT'C
Cuts at: 0 845 2706
Size: 845 1861
SexAI A'CCwGG_T
Cuts at: 0 1846 2706
Size: 1846 860
SfcI C'TryA_G
Cuts at: 0 38 379 761 2706
Size: 38 341 382 1945
Fragments arranged by size:
1945 382 341 38
SmlI C'TyrA_G
Cuts at: 0 812 950 968 1599 2520 2706
Size: 812 138 18 631 921 186
Fragments arranged by size:
921 812 631 186 138 18
SspI AAT'ATT
Cuts at: 0 2675 2706
Size: 2675 31
StuI AGG'CCT
Cuts at: 0 1612 2706
Size: 1612 1094
StyI C'CwwG_G
Cuts at: 0 831 1245 1518 1857 2706
Size: 831 414 273 339 849
Fragments arranged by size:
849 831 414 339 273
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2562 2706
Size: 2562 144
TatI w'GTAC_w
Cuts at: 0 102 2074 2706
Size: 102 1972 632
Fragments arranged by size:
1972 632 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1439 1471 2045 2077 2706
Size: 1439 32 574 32 629
Fragments arranged by size:
1439 629 574 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2323 2706
Size: 2323 383
XbaI T'CTAG_A
Cuts at: 0 1108 2706
Size: 1108 1598
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2564 2706
Size: 2564 142
XhoI C'TCGA_G
Cuts at: 0 968 2706
Size: 968 1738
XmnI GAAnn'nnTTC
Cuts at: 0 615 2093 2706
Size: 615 1478 613
Fragments arranged by size:
1478 615 613
ZraI GAC'GTC
Cuts at: 0 2656 2706
Size: 2656 50
Enzymes that do cut:
AatII AccI AcuI AflIII AloI AlwNI ApoI AvaI
AvrII BanII BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrFI
BsrGI BstYI BtgI ClaI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I HincII HindIII
HpaI MscI MslI MspA1I NcoI NdeI NsiI PasI
PflMI PfoI PmlI PpiI PpuMI PshAI PsiI PspXI
PstI PvuII SacI SexAI SfcI SmlI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI ApaI ApaLI AscI AseI AsiSI BaeI BamHI
BanI BbeI BcgI BglI BmgBI BmrI BmtI BplI
BsaI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspMI
BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI CspCI DrdI EagI EciI
EcoNI EcoRV FseI FspI FspAI HaeII KasI KpnI
MfeI MluI MmeI NaeI NarI NgoMIV NheI NotI
NruI NspI PacI PciI PmeI PspOMI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI XmaI