Restriction map for
pFN21AB8542
(Linear) (Six-base) MAPSORT of: KIBB8542.seq Check: 5115 from: 1 to: 2700
KIBB8542.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1939 2251 2693 2700
Size: 1939 312 442 7
Fragments arranged by size:
1939 442 312 7
AfeI AGC'GCT
Cuts at: 0 2446 2531 2700
Size: 2446 85 169
Fragments arranged by size:
2446 169 85
AflII C'TTAA_G
Cuts at: 0 1288 2700
Size: 1288 1412
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2356 2700
Size: 2356 344
AlwNI CAG_nnn'CTG
Cuts at: 0 252 525 562 1119 1347 1418 2040 2224
Size: 252 273 37 557 228 71 622 184
Cuts at: 2224 2700
Size: 476
Fragments arranged by size:
622 557 476 273 252 228 184 71
37
ApoI r'AATT_y
Cuts at: 0 1777 2700
Size: 1777 923
AvaI C'yCGr_G
Cuts at: 0 27 65 1238 1487 2700
Size: 27 38 1173 249 1213
Fragments arranged by size:
1213 1173 249 38 27
AvrII C'CTAG_G
Cuts at: 0 1704 2700
Size: 1704 996
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1496 1529 2700
Size: 1496 33 1171
Fragments arranged by size:
1496 1171 33
BamHI G'GATC_C
Cuts at: 0 1832 2099 2700
Size: 1832 267 601
Fragments arranged by size:
1832 601 267
BanII G_rGCy'C
Cuts at: 0 66 360 453 2066 2171 2700
Size: 66 294 93 1613 105 529
Fragments arranged by size:
1613 529 294 105 93 66
BbsI GAAGACnn'nnnn_
Cuts at: 0 1538 2700
Size: 1538 1162
BbvCI CC'TCA_GC
Cuts at: 0 632 1712 2657 2700
Size: 632 1080 945 43
Fragments arranged by size:
1080 945 632 43
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 255 289 862 896 1159 1193 2590 2624
Size: 255 34 573 34 263 34 1397 34
Cuts at: 2624 2700
Size: 76
Fragments arranged by size:
1397 573 263 255 76 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2131 2347 2700
Size: 2131 216 353
Fragments arranged by size:
2131 353 216
BclI T'GATC_A
Cuts at: 0 2031 2700
Size: 2031 669
BglII A'GATC_T
Cuts at: 0 992 2700
Size: 992 1708
BlpI GC'TnA_GC
Cuts at: 0 1698 2700
Size: 1698 1002
Bme1580I G_kGCm'C
Cuts at: 0 504 813 2700
Size: 504 309 1887
Fragments arranged by size:
1887 504 309
BmrI ACTGGGnnnn_n'
Cuts at: 0 1428 2700
Size: 1428 1272
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 988 1390 1402 2050 2080 2309 2700
Size: 803 185 402 12 648 30 229 391
Fragments arranged by size:
803 648 402 391 229 185 30 12
Bpu10I CC'TnA_GC
Cuts at: 0 632 1364 1712 2657 2700
Size: 632 732 348 945 43
Fragments arranged by size:
945 732 632 348 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 1453 2107 2700
Size: 402 1051 654 593
Fragments arranged by size:
1051 654 593 402
BsaI GGTCTCn'nnnn_
Cuts at: 0 949 1484 2700
Size: 949 535 1216
Fragments arranged by size:
1216 949 535
BsaHI Gr'CG_yC
Cuts at: 0 23 2700
Size: 23 2677
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 163 193 1059 1089 1195 1225 1375 1405
Size: 163 30 866 30 106 30 150 30
Cuts at: 1405 1600 1630 2700
Size: 195 30 1070
Fragments arranged by size:
1070 866 195 163 150 106 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 505 676 679 682 703 706 985 1246
Size: 505 171 3 3 21 3 279 261
Cuts at: 1246 1399 2700
Size: 153 1301
Fragments arranged by size:
1301 505 279 261 171 153 21 3
3 3
BseYI C'CCAG_C
Cuts at: 0 221 409 617 622 755 1969 2306 2700
Size: 221 188 208 5 133 1214 337 394
Fragments arranged by size:
1214 394 337 221 208 188 133 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 1351 2700
Size: 490 861 1349
Fragments arranged by size:
1349 861 490
BsiHKAI G_wGCw'C
Cuts at: 0 86 800 866 1172 1179 2021 2066 2700
Size: 86 714 66 306 7 842 45 634
Fragments arranged by size:
842 714 634 306 86 66 45 7
Bsp1286I G_dGCh'C
Cuts at: 0 66 86 360 453 504 800 813 866
Size: 66 20 274 93 51 296 13 53
Cuts at: 866 1172 1179 2021 2066 2171 2700
Size: 306 7 842 45 105 529
Fragments arranged by size:
842 529 306 296 274 105 93 66
53 51 45 20 13 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1367 1919 2700
Size: 1367 552 781
Fragments arranged by size:
1367 781 552
BsrDI GCAATG_nn'
Cuts at: 0 1402 1742 2700
Size: 1402 340 958
Fragments arranged by size:
1402 958 340
BsrFI r'CCGG_y
Cuts at: 0 39 2700
Size: 39 2661
BsrGI T'GTAC_A
Cuts at: 0 1865 2700
Size: 1865 835
BstEII G'GTnAC_C
Cuts at: 0 1243 2700
Size: 1243 1457
BstXI CCAn_nnnn'nTGG
Cuts at: 0 966 2700
Size: 966 1734
BstYI r'GATC_y
Cuts at: 0 471 992 1832 2099 2631 2700
Size: 471 521 840 267 532 69
Fragments arranged by size:
840 532 521 471 267 69
Bsu36I CC'TnA_GG
Cuts at: 0 137 488 659 1877 2700
Size: 137 351 171 1218 823
Fragments arranged by size:
1218 823 351 171 137
BtgI C'CryG_G
Cuts at: 0 1791 2282 2700
Size: 1791 491 418
Fragments arranged by size:
1791 491 418
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1176 2700
Size: 1176 1524
BtsI GCAGTG_nn'
Cuts at: 0 1419 2196 2240 2700
Size: 1419 777 44 460
Fragments arranged by size:
1419 777 460 44
EaeI y'GGCC_r
Cuts at: 0 47 1655 2700
Size: 47 1608 1045
Fragments arranged by size:
1608 1045 47
EarI CTCTTCn'nnn_
Cuts at: 0 181 2700
Size: 181 2519
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 889 2700
Size: 889 1811
EcoICRI GAG'CTC
Cuts at: 0 2064 2700
Size: 2064 636
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 988 1390 1402 1939 2050 2080 2251
Size: 803 185 402 12 537 111 30 171
Cuts at: 2251 2309 2693 2700
Size: 58 384 7
Fragments arranged by size:
803 537 402 384 185 171 111 58
30 12 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 487 685 2700
Size: 487 198 2015
Fragments arranged by size:
2015 487 198
EcoO109I rG'GnC_Cy
Cuts at: 0 141 726 2700
Size: 141 585 1974
Fragments arranged by size:
1974 585 141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 355 2700
Size: 355 2345
FspI TGC'GCA
Cuts at: 0 2116 2700
Size: 2116 584
HaeII r_GCGC'y
Cuts at: 0 1854 2040 2448 2533 2700
Size: 1854 186 408 85 167
Fragments arranged by size:
1854 408 186 167 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 406 408 438 440 948 980 2700
Size: 406 2 30 2 508 32 1720
Fragments arranged by size:
1720 508 406 32 30 2 2
HincII GTy'rAC
Cuts at: 0 825 2700
Size: 825 1875
MscI TGG'CCA
Cuts at: 0 1657 2700
Size: 1657 1043
MspA1I CmG'CkG
Cuts at: 0 156 512 631 747 1034 1793 2310 2700
Size: 156 356 119 116 287 759 517 390
Fragments arranged by size:
759 517 390 356 287 156 119 116
NspI r_CATG'y
Cuts at: 0 2280 2700
Size: 2280 420
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 2412 2700
Size: 252 2160 288
Fragments arranged by size:
2160 288 252
PfoI T'CCnGG_A
Cuts at: 0 1900 2102 2700
Size: 1900 202 598
Fragments arranged by size:
1900 598 202
PpuMI rG'GwC_Cy
Cuts at: 0 141 2700
Size: 141 2559
PstI C_TGCA'G
Cuts at: 0 278 558 2315 2335 2700
Size: 278 280 1757 20 365
Fragments arranged by size:
1757 365 280 278 20
PvuII CAG'CTG
Cuts at: 0 156 512 2310 2700
Size: 156 356 1798 390
Fragments arranged by size:
1798 390 356 156
SacI G_AGCT'C
Cuts at: 0 2066 2700
Size: 2066 634
SacII CC_GC'GG
Cuts at: 0 1794 2700
Size: 1794 906
SfcI C'TryA_G
Cuts at: 0 95 274 554 1748 2311 2331 2651 2700
Size: 95 179 280 1194 563 20 320 49
Fragments arranged by size:
1194 563 320 280 179 95 49 20
SmaI CCC'GGG
Cuts at: 0 29 67 2700
Size: 29 38 2633
Fragments arranged by size:
2633 38 29
SmlI C'TyrA_G
Cuts at: 0 381 1288 1468 2086 2700
Size: 381 907 180 618 614
Fragments arranged by size:
907 618 614 381 180
SpeI A'CTAG_T
Cuts at: 0 184 2700
Size: 184 2516
StyI C'CwwG_G
Cuts at: 0 315 840 959 1704 1727 2700
Size: 315 525 119 745 23 973
Fragments arranged by size:
973 745 525 315 119 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1111 1440 2700
Size: 1111 329 1260
Fragments arranged by size:
1260 1111 329
TatI w'GTAC_w
Cuts at: 0 299 564 1865 2700
Size: 299 265 1301 835
Fragments arranged by size:
1301 835 299 265
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1059 1091 1195 1227 1600 1632 2379 2411
Size: 1059 32 104 32 373 32 747 32
Cuts at: 2411 2700
Size: 289
Fragments arranged by size:
1059 747 373 289 104 32 32 32
32
XmaI C'CCGG_G
Cuts at: 0 27 65 2700
Size: 27 38 2635
Fragments arranged by size:
2635 38 27
XmnI GAAnn'nnTTC
Cuts at: 0 335 2700
Size: 335 2365
Enzymes that do cut:
AcuI AfeI AflII AlfI AlwNI ApoI AvaI AvrII
BaeI BamHI BanII BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrDI BsrFI BsrGI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I HincII MscI MspA1I
NspI PflMI PfoI PpuMI PstI PvuII SacI SacII
SfcI SmaI SmlI SpeI StyI TaqII TatI TstI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI BanI
BbeI BfrBI BglI BmgBI BmtI BplI BsaAI BsaBI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BssHII BssSI BstAPI BstBI BstZ17I ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FseI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI ZraI