Restriction map for
pFN21AB8541
(Linear) (Six-base) MAPSORT of: KIBB8541.seq Check: 7213 from: 1 to: 2937
KIBB8541.orf
With 182 enzymes: SgfI *
July 3, 2012 16:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 569 813 1257 1744 2114 2937
Size: 569 244 444 487 370 823
Fragments arranged by size:
823 569 487 444 370 244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 714 734 2278 2587 2937
Size: 714 20 1544 309 350
Fragments arranged by size:
1544 714 350 309 20
AflIII A'CryG_T
Cuts at: 0 1493 2937
Size: 1493 1444
AleI CACnn'nnGTG
Cuts at: 0 1741 2726 2937
Size: 1741 985 211
Fragments arranged by size:
1741 985 211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1010 2937
Size: 1010 1927
AlwNI CAG_nnn'CTG
Cuts at: 0 137 1011 1098 1572 1890 2937
Size: 137 874 87 474 318 1047
Fragments arranged by size:
1047 874 474 318 137 87
ApaI G_GGCC'C
Cuts at: 0 1860 2356 2937
Size: 1860 496 581
Fragments arranged by size:
1860 581 496
ApaLI G'TGCA_C
Cuts at: 0 1489 2719 2937
Size: 1489 1230 218
Fragments arranged by size:
1489 1230 218
AvaI C'yCGr_G
Cuts at: 0 28 84 2937
Size: 28 56 2853
Fragments arranged by size:
2853 56 28
BamHI G'GATC_C
Cuts at: 0 34 2937
Size: 34 2903
BanI G'GyrC_C
Cuts at: 0 5 387 876 1269 2482 2749 2937
Size: 5 382 489 393 1213 267 188
Fragments arranged by size:
1213 489 393 382 267 188 5
BanII G_rGCy'C
Cuts at: 0 362 676 1640 1860 2356 2937
Size: 362 314 964 220 496 581
Fragments arranged by size:
964 581 496 362 314 220
BbeI G_GCGC'C
Cuts at: 0 9 2937
Size: 9 2928
BbvCI CC'TCA_GC
Cuts at: 0 1386 2617 2937
Size: 1386 1231 320
Fragments arranged by size:
1386 1231 320
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2305 2339 2397 2431 2937
Size: 2305 34 58 34 506
Fragments arranged by size:
2305 506 58 34 34
BclI T'GATC_A
Cuts at: 0 1428 2937
Size: 1428 1509
BglI GCCn_nnn'nGGC
Cuts at: 0 89 1352 2937
Size: 89 1263 1585
Fragments arranged by size:
1585 1263 89
BlpI GC'TnA_GC
Cuts at: 0 73 533 1481 2937
Size: 73 460 948 1456
Fragments arranged by size:
1456 948 460 73
Bme1580I G_kGCm'C
Cuts at: 0 111 1493 1860 2356 2638 2723 2937
Size: 111 1382 367 496 282 85 214
Fragments arranged by size:
1382 496 367 282 214 111 85
BmrI ACTGGGnnnn_n'
Cuts at: 0 226 300 1360 1971 2937
Size: 226 74 1060 611 966
Fragments arranged by size:
1060 966 611 226 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1086 2502 2937
Size: 1086 1416 435
Fragments arranged by size:
1416 1086 435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 642 1236 1489 2845 2937
Size: 431 211 594 253 1356 92
Fragments arranged by size:
1356 594 431 253 211 92
Bpu10I CC'TnA_GC
Cuts at: 0 1061 1386 1441 2617 2937
Size: 1061 325 55 1176 320
Fragments arranged by size:
1176 1061 325 320 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2905 2937
Size: 2905 32
BsaI GGTCTCn'nnnn_
Cuts at: 0 31 49 390 669 2937
Size: 31 18 341 279 2268
Fragments arranged by size:
2268 341 279 31 18
BsaAI yAC'GTr
Cuts at: 0 2499 2937
Size: 2499 438
BsaBI GATnn'nnATC
Cuts at: 0 2809 2937
Size: 2809 128
BsaHI Gr'CG_yC
Cuts at: 0 6 2937
Size: 6 2931
BsaWI w'CCGG_w
Cuts at: 0 1944 2937
Size: 1944 993
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1118 1148 2937
Size: 1118 30 1789
Fragments arranged by size:
1789 1118 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 501 1275 1687 2053 2937
Size: 46 455 774 412 366 884
Fragments arranged by size:
884 774 455 412 366 46
BseYI C'CCAG_C
Cuts at: 0 458 499 809 862 978 1395 1762 2005
Size: 458 41 310 53 116 417 367 243
Cuts at: 2005 2937
Size: 932
Fragments arranged by size:
932 458 417 367 310 243 116 53
41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1130 2391 2596 2937
Size: 1006 124 1261 205 341
Fragments arranged by size:
1261 1006 341 205 124
BsiHKAI G_wGCw'C
Cuts at: 0 260 560 1493 2528 2723 2937
Size: 260 300 933 1035 195 214
Fragments arranged by size:
1035 933 300 260 214 195
BsmI GAATG_Cn'
Cuts at: 0 2916 2937
Size: 2916 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2581 2937
Size: 2581 356
Bsp1286I G_dGCh'C
Cuts at: 0 111 260 362 560 676 1493 1640 1860
Size: 111 149 102 198 116 817 147 220
Cuts at: 1860 2356 2528 2638 2723 2937
Size: 496 172 110 85 214
Fragments arranged by size:
817 496 220 214 198 172 149 147
116 111 110 102 85
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 569 615 813 1257 1744 2114 2937
Size: 569 46 198 444 487 370 823
Fragments arranged by size:
823 569 487 444 370 198 46
BsrBI CCG'CTC
Cuts at: 0 2402 2702 2861 2937
Size: 2402 300 159 76
Fragments arranged by size:
2402 300 159 76
BsrFI r'CCGG_y
Cuts at: 0 1173 2043 2213 2625 2640 2746 2937
Size: 1173 870 170 412 15 106 191
Fragments arranged by size:
1173 870 412 191 170 106 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 137 564 1098 2937
Size: 137 427 534 1839
Fragments arranged by size:
1839 534 427 137
BstXI CCAn_nnnn'nTGG
Cuts at: 0 640 2848 2937
Size: 640 2208 89
Fragments arranged by size:
2208 640 89
BstYI r'GATC_y
Cuts at: 0 34 1472 2937
Size: 34 1438 1465
Fragments arranged by size:
1465 1438 34
Bsu36I CC'TnA_GG
Cuts at: 0 1500 1850 2937
Size: 1500 350 1087
Fragments arranged by size:
1500 1087 350
BtgI C'CryG_G
Cuts at: 0 10 111 1367 1860 2069 2894 2937
Size: 10 101 1256 493 209 825 43
Fragments arranged by size:
1256 825 493 209 101 43 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2355 2796 2937
Size: 2355 441 141
Fragments arranged by size:
2355 441 141
BtsI GCAGTG_nn'
Cuts at: 0 187 1235 1971 2461 2721 2733 2937
Size: 187 1048 736 490 260 12 204
Fragments arranged by size:
1048 736 490 260 204 187 12
EaeI y'GGCC_r
Cuts at: 0 771 1013 1907 2470 2623 2937
Size: 771 242 894 563 153 314
Fragments arranged by size:
894 771 563 314 242 153
EarI CTCTTCn'nnn_
Cuts at: 0 733 2937
Size: 733 2204
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 435 2914 2937
Size: 435 2479 23
Fragments arranged by size:
2479 435 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 642 714 734 1236 1489 2278 2587
Size: 431 211 72 20 502 253 789 309
Cuts at: 2587 2845 2937
Size: 258 92
Fragments arranged by size:
789 502 431 309 258 253 211 92
72 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 706 2128 2937
Size: 706 1422 809
Fragments arranged by size:
1422 809 706
EcoO109I rG'GnC_Cy
Cuts at: 0 786 796 1846 1856 1887 2142 2352 2925
Size: 786 10 1050 10 31 255 210 573
Cuts at: 2925 2937
Size: 12
Fragments arranged by size:
1050 786 573 255 210 31 12 10
10
EcoRV GAT'ATC
Cuts at: 0 1152 2937
Size: 1152 1785
FspI TGC'GCA
Cuts at: 0 1703 2937
Size: 1703 1234
HaeII r_GCGC'y
Cuts at: 0 9 101 1833 2937
Size: 9 92 1732 1104
Fragments arranged by size:
1732 1104 92 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 387 419 1136 1168 1523 1555 2937
Size: 387 32 717 32 355 32 1382
Fragments arranged by size:
1382 717 387 355 32 32 32
KasI G'GCGC_C
Cuts at: 0 5 2937
Size: 5 2932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 285 1122 2501 2937
Size: 264 21 837 1379 436
Fragments arranged by size:
1379 837 436 264 21
MscI TGG'CCA
Cuts at: 0 773 1909 2472 2937
Size: 773 1136 563 465
Fragments arranged by size:
1136 773 563 465
MslI CAynn'nnrTG
Cuts at: 0 1522 1741 2419 2501 2716 2726 2767 2834
Size: 1522 219 678 82 215 10 41 67
Cuts at: 2834 2937
Size: 103
Fragments arranged by size:
1522 678 219 215 103 82 67 41
10
MspA1I CmG'CkG
Cuts at: 0 12 982 1018 1053 1681 2358 2433 2621
Size: 12 970 36 35 628 677 75 188
Cuts at: 2621 2844 2937
Size: 223 93
Fragments arranged by size:
970 677 628 223 188 93 75 36
35 12
NaeI GCC'GGC
Cuts at: 0 2045 2627 2937
Size: 2045 582 310
Fragments arranged by size:
2045 582 310
NarI GG'CG_CC
Cuts at: 0 6 2937
Size: 6 2931
NcoI C'CATG_G
Cuts at: 0 1860 2937
Size: 1860 1077
NgoMIV G'CCGG_C
Cuts at: 0 2043 2625 2937
Size: 2043 582 312
Fragments arranged by size:
2043 582 312
NspI r_CATG'y
Cuts at: 0 1489 1497 1810 2833 2937
Size: 1489 8 313 1023 104
Fragments arranged by size:
1489 1023 313 104 8
PasI CC'CwG_GG
Cuts at: 0 679 1842 2937
Size: 679 1163 1095
Fragments arranged by size:
1163 1095 679
PciI A'CATG_T
Cuts at: 0 1493 2937
Size: 1493 1444
PflMI CCAn_nnn'nTGG
Cuts at: 0 1905 2135 2937
Size: 1905 230 802
Fragments arranged by size:
1905 802 230
PfoI T'CCnGG_A
Cuts at: 0 1122 2937
Size: 1122 1815
PpuMI rG'GwC_Cy
Cuts at: 0 786 1887 2937
Size: 786 1101 1050
Fragments arranged by size:
1101 1050 786
PshAI GACnn'nnGTC
Cuts at: 0 1324 2937
Size: 1324 1613
PspOMI G'GGCC_C
Cuts at: 0 1856 2352 2937
Size: 1856 496 585
Fragments arranged by size:
1856 585 496
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2674 2706 2937
Size: 2674 32 231
Fragments arranged by size:
2674 231 32
PstI C_TGCA'G
Cuts at: 0 97 530 620 1007 1094 1455 1686 2728
Size: 97 433 90 387 87 361 231 1042
Cuts at: 2728 2937
Size: 209
Fragments arranged by size:
1042 433 387 361 231 209 97 90
87
PvuII CAG'CTG
Cuts at: 0 982 1053 1681 2621 2937
Size: 982 71 628 940 316
Fragments arranged by size:
982 940 628 316 71
SacII CC_GC'GG
Cuts at: 0 13 2937
Size: 13 2924
SexAI A'CCwGG_T
Cuts at: 0 399 2513 2937
Size: 399 2114 424
Fragments arranged by size:
2114 424 399
SfcI C'TryA_G
Cuts at: 0 93 526 616 1003 1090 1451 1682 2724
Size: 93 433 90 387 87 361 231 1042
Cuts at: 2724 2937
Size: 213
Fragments arranged by size:
1042 433 387 361 231 213 93 90
87
SfoI GGC'GCC
Cuts at: 0 7 2937
Size: 7 2930
SmaI CCC'GGG
Cuts at: 0 86 2937
Size: 86 2851
SmlI C'TyrA_G
Cuts at: 0 2920 2937
Size: 2920 17
SphI G_CATG'C
Cuts at: 0 1810 2937
Size: 1810 1127
StyI C'CwwG_G
Cuts at: 0 276 367 707 746 880 1103 1860 2129
Size: 276 91 340 39 134 223 757 269
Cuts at: 2129 2937
Size: 808
Fragments arranged by size:
808 757 340 276 269 223 134 91
39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 319 405 876 1731 2937
Size: 319 86 471 855 1206
Fragments arranged by size:
1206 855 471 319 86
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 675 707 2937
Size: 675 32 2230
Fragments arranged by size:
2230 675 32
Tth111I GACn'n_nGTC
Cuts at: 0 401 2770 2937
Size: 401 2369 167
Fragments arranged by size:
2369 401 167
XmaI C'CCGG_G
Cuts at: 0 84 2937
Size: 84 2853
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BclI
BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PpuMI
PshAI PspOMI PsrI PstI PvuII SacII SexAI SfcI
SfoI SmaI SmlI SphI StyI TaqII TstI Tth111I
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApoI AscI AseI AsiSI AvrII BaeI BbsI
BciVI BfrBI BglII BmgBI BmtI BsiEI BsiWI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PsiI PspXI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI StuI SwaI TatI XbaI XcmI
XhoI XmnI ZraI