Restriction map for
pFN21AB8539
(Linear) (Six-base) MAPSORT of: KIBB8539.seq Check: 5898 from: 1 to: 2865
KIBB8539.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 590 2367 2385 2394 2736 2865
Size: 590 1777 18 9 342 129
Fragments arranged by size:
1777 590 342 129 18 9
AatII G_ACGT'C
Cuts at: 0 1148 1424 1781 2054 2865
Size: 1148 276 357 273 811
Fragments arranged by size:
1148 811 357 276 273
AccI GT'mk_AC
Cuts at: 0 1954 2865
Size: 1954 911
Acc65I G'GTAC_C
Cuts at: 0 1232 1385 2865
Size: 1232 153 1480
Fragments arranged by size:
1480 1232 153
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1297 2518 2865
Size: 1297 1221 347
Fragments arranged by size:
1297 1221 347
AfeI AGC'GCT
Cuts at: 0 2566 2865
Size: 2566 299
AleI CACnn'nnGTG
Cuts at: 0 602 2840 2865
Size: 602 2238 25
Fragments arranged by size:
2238 602 25
AlwNI CAG_nnn'CTG
Cuts at: 0 1011 2122 2214 2250 2865
Size: 1011 1111 92 36 615
Fragments arranged by size:
1111 1011 615 92 36
ApaI G_GGCC'C
Cuts at: 0 2090 2865
Size: 2090 775
ApaLI G'TGCA_C
Cuts at: 0 1059 2865
Size: 1059 1806
ApoI r'AATT_y
Cuts at: 0 2301 2865
Size: 2301 564
AvaI C'yCGr_G
Cuts at: 0 1657 1816 2095 2325 2865
Size: 1657 159 279 230 540
Fragments arranged by size:
1657 540 279 230 159
BanI G'GyrC_C
Cuts at: 0 546 1232 1385 2476 2865
Size: 546 686 153 1091 389
Fragments arranged by size:
1091 686 546 389 153
BanII G_rGCy'C
Cuts at: 0 38 881 971 1937 2090 2774 2825 2865
Size: 38 843 90 966 153 684 51 40
Fragments arranged by size:
966 843 684 153 90 51 40 38
BbeI G_GCGC'C
Cuts at: 0 2480 2865
Size: 2480 385
BbsI GAAGACnn'nnnn_
Cuts at: 0 2046 2865
Size: 2046 819
BbvCI CC'TCA_GC
Cuts at: 0 1756 2110 2865
Size: 1756 354 755
Fragments arranged by size:
1756 755 354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 421 455 2865
Size: 421 34 2410
Fragments arranged by size:
2410 421 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1291 1612 2485 2865
Size: 1291 321 873 380
Fragments arranged by size:
1291 873 380 321
BlpI GC'TnA_GC
Cuts at: 0 2578 2865
Size: 2578 287
Bme1580I G_kGCm'C
Cuts at: 0 227 551 1063 2090 2865
Size: 227 324 512 1027 775
Fragments arranged by size:
1027 775 512 324 227
BmgBI CAC'GTC
Cuts at: 0 671 1719 2865
Size: 671 1048 1146
Fragments arranged by size:
1146 1048 671
BmrI ACTGGGnnnn_n'
Cuts at: 0 1552 2500 2865
Size: 1552 948 365
Fragments arranged by size:
1552 948 365
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2603 2844 2865
Size: 2603 241 21
Fragments arranged by size:
2603 241 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 794 871 1069 1258 1444 1533 1816
Size: 715 79 77 198 189 186 89 283
Cuts at: 1816 2320 2788 2865
Size: 504 468 77
Fragments arranged by size:
715 504 468 283 198 189 186 89
79 77 77
Bpu10I CC'TnA_GC
Cuts at: 0 697 1756 2110 2865
Size: 697 1059 354 755
Fragments arranged by size:
1059 755 697 354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 2865
Size: 1114 1751
BsaI GGTCTCn'nnnn_
Cuts at: 0 928 1018 1654 2324 2865
Size: 928 90 636 670 541
Fragments arranged by size:
928 670 636 541 90
BsaAI yAC'GTr
Cuts at: 0 1326 1368 2865
Size: 1326 42 1497
Fragments arranged by size:
1497 1326 42
BsaHI Gr'CG_yC
Cuts at: 0 1145 1421 1682 1778 1957 2051 2440 2477
Size: 1145 276 261 96 179 94 389 37
Cuts at: 2477 2865
Size: 388
Fragments arranged by size:
1145 389 388 276 261 179 96 94
37
BsaWI w'CCGG_w
Cuts at: 0 59 1193 2145 2465 2865
Size: 59 1134 952 320 400
Fragments arranged by size:
1134 952 400 320 59
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 106 136 1042 1072 1369 1399 1531 1561
Size: 106 30 906 30 297 30 132 30
Cuts at: 1561 2865
Size: 1304
Fragments arranged by size:
1304 906 297 132 106 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 702 1165 1279 1390 1813 2113 2865
Size: 702 463 114 111 423 300 752
Fragments arranged by size:
752 702 463 423 300 114 111
BseYI C'CCAG_C
Cuts at: 0 756 999 1534 1700 1903 1942 2072 2077
Size: 756 243 535 166 203 39 130 5
Cuts at: 2077 2865
Size: 788
Fragments arranged by size:
788 756 535 243 203 166 130 39
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 919 1582 1617 2311 2374 2419 2865
Size: 379 540 663 35 694 63 45 446
Fragments arranged by size:
694 663 540 446 379 63 45 35
BsiEI CG_ry'CG
Cuts at: 0 8 617 2069 2865
Size: 8 609 1452 796
Fragments arranged by size:
1452 796 609 8
BsiHKAI G_wGCw'C
Cuts at: 0 863 1063 2411 2865
Size: 863 200 1348 454
Fragments arranged by size:
1348 863 454 200
BsmI GAATG_Cn'
Cuts at: 0 903 2865
Size: 903 1962
BsmBI CGTCTCn'nnnn_
Cuts at: 0 681 714 1234 2832 2865
Size: 681 33 520 1598 33
Fragments arranged by size:
1598 681 520 33 33
Bsp1286I G_dGCh'C
Cuts at: 0 38 227 551 863 881 971 1063 1937
Size: 38 189 324 312 18 90 92 874
Cuts at: 1937 2090 2411 2774 2825 2865
Size: 153 321 363 51 40
Fragments arranged by size:
874 363 324 321 312 189 153 92
90 51 40 38 18
BspEI T'CCGG_A
Cuts at: 0 59 2145 2865
Size: 59 2086 720
Fragments arranged by size:
2086 720 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 590 1040 2367 2385 2394 2736 2865
Size: 590 450 1327 18 9 342 129
Fragments arranged by size:
1327 590 450 342 129 18 9
BsrBI CCG'CTC
Cuts at: 0 122 1140 1547 2865
Size: 122 1018 407 1318
Fragments arranged by size:
1318 1018 407 122
BsrDI GCAATG_nn'
Cuts at: 0 1206 1352 1716 2865
Size: 1206 146 364 1149
Fragments arranged by size:
1206 1149 364 146
BsrFI r'CCGG_y
Cuts at: 0 3 1693 1870 2795 2865
Size: 3 1690 177 925 70
Fragments arranged by size:
1690 925 177 70 3
BsrGI T'GTAC_A
Cuts at: 0 524 2865
Size: 524 2341
BssHII G'CGCG_C
Cuts at: 0 2390 2865
Size: 2390 475
BssSI C'ACGA_G
Cuts at: 0 1477 2865
Size: 1477 1388
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2406 2865
Size: 2406 459
BstEII G'GTnAC_C
Cuts at: 0 280 2865
Size: 280 2585
BstXI CCAn_nnnn'nTGG
Cuts at: 0 764 978 1794 2367 2541 2658 2865
Size: 764 214 816 573 174 117 207
Fragments arranged by size:
816 764 573 214 207 174 117
Bsu36I CC'TnA_GG
Cuts at: 0 2123 2865
Size: 2123 742
BtgI C'CryG_G
Cuts at: 0 551 1307 1739 1787 2627 2865
Size: 551 756 432 48 840 238
Fragments arranged by size:
840 756 551 432 238 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 35 387 2865
Size: 35 352 2478
Fragments arranged by size:
2478 352 35
BtsI GCAGTG_nn'
Cuts at: 0 396 802 2865
Size: 396 406 2063
Fragments arranged by size:
2063 406 396
EaeI y'GGCC_r
Cuts at: 0 5 110 204 359 467 614 1067 1283
Size: 5 105 94 155 108 147 453 216
Cuts at: 1283 1292 1604 1766 1784 1838 1890 2066 2865
Size: 9 312 162 18 54 52 176 799
Fragments arranged by size:
799 453 312 216 176 162 155 147
108 105 94 54 52 18 9 5
EagI C'GGCC_G
Cuts at: 0 5 614 2066 2865
Size: 5 609 1452 799
Fragments arranged by size:
1452 799 609 5
EarI CTCTTCn'nnn_
Cuts at: 0 934 1657 2272 2865
Size: 934 723 615 593
Fragments arranged by size:
934 723 615 593
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 97 316 1959 2865
Size: 97 219 1643 906
Fragments arranged by size:
1643 906 219 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 794 871 1069 1258 1297 1444 1533
Size: 715 79 77 198 189 39 147 89
Cuts at: 1533 1816 2320 2518 2788 2865
Size: 283 504 198 270 77
Fragments arranged by size:
715 504 283 270 198 198 189 147
89 79 77 77 39
EcoO109I rG'GnC_Cy
Cuts at: 0 1464 1539 1899 2086 2119 2193 2487 2865
Size: 1464 75 360 187 33 74 294 378
Fragments arranged by size:
1464 378 360 294 187 75 74 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1464 2865
Size: 1464 1401
FspI TGC'GCA
Cuts at: 0 1708 2809 2865
Size: 1708 1101 56
Fragments arranged by size:
1708 1101 56
HaeII r_GCGC'y
Cuts at: 0 1459 1618 1880 2480 2568 2865
Size: 1459 159 262 600 88 297
Fragments arranged by size:
1459 600 297 262 159 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1531 1563 1810 1842 2865
Size: 1531 32 247 32 1023
Fragments arranged by size:
1531 1023 247 32 32
HincII GTy'rAC
Cuts at: 0 1955 2865
Size: 1955 910
HindIII A'AGCT_T
Cuts at: 0 2653 2865
Size: 2653 212
KasI G'GCGC_C
Cuts at: 0 2476 2865
Size: 2476 389
KpnI G_GTAC'C
Cuts at: 0 1236 1389 2865
Size: 1236 153 1476
Fragments arranged by size:
1476 1236 153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 932 1199 2265 2865
Size: 77 855 267 1066 600
Fragments arranged by size:
1066 855 600 267 77
MscI TGG'CCA
Cuts at: 0 112 206 361 1069 1285 1606 1768 1786
Size: 112 94 155 708 216 321 162 18
Cuts at: 1786 1840 2865
Size: 54 1025
Fragments arranged by size:
1025 708 321 216 162 155 112 94
54 18
MslI CAynn'nnrTG
Cuts at: 0 295 379 463 602 604 2539 2840 2865
Size: 295 84 84 139 2 1935 301 25
Fragments arranged by size:
1935 301 295 139 84 84 25 2
MspA1I CmG'CkG
Cuts at: 0 445 999 1161 1319 1704 2211 2346 2448
Size: 445 554 162 158 385 507 135 102
Cuts at: 2448 2577 2865
Size: 129 288
Fragments arranged by size:
554 507 445 385 288 162 158 135
129 102
NaeI GCC'GGC
Cuts at: 0 5 1695 1872 2865
Size: 5 1690 177 993
Fragments arranged by size:
1690 993 177 5
NarI GG'CG_CC
Cuts at: 0 2477 2865
Size: 2477 388
NcoI C'CATG_G
Cuts at: 0 551 2627 2865
Size: 551 2076 238
Fragments arranged by size:
2076 551 238
NgoMIV G'CCGG_C
Cuts at: 0 3 1693 1870 2865
Size: 3 1690 177 995
Fragments arranged by size:
1690 995 177 3
NspI r_CATG'y
Cuts at: 0 1059 2262 2865
Size: 1059 1203 603
Fragments arranged by size:
1203 1059 603
PflMI CCAn_nnn'nTGG
Cuts at: 0 600 2628 2865
Size: 600 2028 237
Fragments arranged by size:
2028 600 237
PfoI T'CCnGG_A
Cuts at: 0 731 2678 2865
Size: 731 1947 187
Fragments arranged by size:
1947 731 187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 2865
Size: 298 32 2535
Fragments arranged by size:
2535 298 32
PpuMI rG'GwC_Cy
Cuts at: 0 1539 1899 2119 2193 2865
Size: 1539 360 220 74 672
Fragments arranged by size:
1539 672 360 220 74
PshAI GACnn'nnGTC
Cuts at: 0 673 2865
Size: 673 2192
PspOMI G'GGCC_C
Cuts at: 0 2086 2865
Size: 2086 779
PstI C_TGCA'G
Cuts at: 0 797 1016 1730 2028 2219 2747 2865
Size: 797 219 714 298 191 528 118
Fragments arranged by size:
797 714 528 298 219 191 118
PvuII CAG'CTG
Cuts at: 0 999 1319 1704 2211 2346 2577 2865
Size: 999 320 385 507 135 231 288
Fragments arranged by size:
999 507 385 320 288 231 135
SalI G'TCGA_C
Cuts at: 0 1953 2865
Size: 1953 912
SanDI GG'GwC_CC
Cuts at: 0 1539 1899 2865
Size: 1539 360 966
Fragments arranged by size:
1539 966 360
SbfI CC_TGCA'GG
Cuts at: 0 2747 2865
Size: 2747 118
SexAI A'CCwGG_T
Cuts at: 0 542 2865
Size: 542 2323
SfcI C'TryA_G
Cuts at: 0 156 793 1012 1314 1396 1726 2024 2215
Size: 156 637 219 302 82 330 298 191
Cuts at: 2215 2743 2865
Size: 528 122
Fragments arranged by size:
637 528 330 302 298 219 191 156
122 82
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1291 2865
Size: 1291 1574
SfoI GGC'GCC
Cuts at: 0 2478 2865
Size: 2478 387
SmaI CCC'GGG
Cuts at: 0 2327 2865
Size: 2327 538
SmlI C'TyrA_G
Cuts at: 0 1129 2865
Size: 1129 1736
StuI AGG'CCT
Cuts at: 0 436 532 2104 2865
Size: 436 96 1572 761
Fragments arranged by size:
1572 761 436 96
StyI C'CwwG_G
Cuts at: 0 14 146 551 1509 1664 2627 2865
Size: 14 132 405 958 155 963 238
Fragments arranged by size:
963 958 405 238 155 132 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 592 2325 2865
Size: 592 1733 540
Fragments arranged by size:
1733 592 540
TatI w'GTAC_w
Cuts at: 0 524 2865
Size: 524 2341
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 555 587 1042 1074 2865
Size: 555 32 455 32 1791
Fragments arranged by size:
1791 555 455 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 487 1114 2865
Size: 487 627 1751
Fragments arranged by size:
1751 627 487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2625 2865
Size: 2625 240
XmaI C'CCGG_G
Cuts at: 0 2325 2865
Size: 2325 540
XmnI GAAnn'nnTTC
Cuts at: 0 2608 2865
Size: 2608 257
ZraI GAC'GTC
Cuts at: 0 1146 1422 1779 2052 2865
Size: 1146 276 357 273 813
Fragments arranged by size:
1146 813 357 276 273
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AleI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI Bsu36I BtgI BtgZI BtsI EaeI
EagI EarI EciI Eco57MI EcoO109I FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PflMI PfoI
PpiI PpuMI PshAI PspOMI PstI PvuII SalI SanDI
SbfI SexAI SfcI SfiI SfoI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AflII AflIII AgeI AhdI AlfI AloI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI
BglII BmtI BsaBI BsiWI BspHI BstBI BstYI BstZ17I
ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI
EcoRV FseI FspAI HpaI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PsiI PspXI PsrI PvuI RsrII SacI SacII SapI
ScaI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI