Restriction map for
pFN21AB8537
(Linear) (Six-base) MAPSORT of: KIBB8537.seq Check: 6226 from: 1 to: 2916
KIBB8537.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1458 1530 2916
Size: 1458 72 1386
Fragments arranged by size:
1458 1386 72
AccI GT'mk_AC
Cuts at: 0 2606 2916
Size: 2606 310
AclI AA'CG_TT
Cuts at: 0 409 2916
Size: 409 2507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1859 2878 2916
Size: 1859 1019 38
Fragments arranged by size:
1859 1019 38
AfeI AGC'GCT
Cuts at: 0 2676 2916
Size: 2676 240
AgeI A'CCGG_T
Cuts at: 0 698 2916
Size: 698 2218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 178 1113 1150 1704 2374 2916
Size: 178 935 37 554 670 542
Fragments arranged by size:
935 670 554 542 178 37
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 264 296 2916
Size: 264 32 2620
Fragments arranged by size:
2620 264 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2028 2409 2718 2769 2916
Size: 2028 381 309 51 147
Fragments arranged by size:
2028 381 309 147 51
ApaI G_GGCC'C
Cuts at: 0 42 953 2314 2916
Size: 42 911 1361 602
Fragments arranged by size:
1361 911 602 42
ApoI r'AATT_y
Cuts at: 0 139 994 1141 2475 2916
Size: 139 855 147 1334 441
Fragments arranged by size:
1334 855 441 147 139
AvaI C'yCGr_G
Cuts at: 0 2333 2916
Size: 2333 583
BamHI G'GATC_C
Cuts at: 0 731 2916
Size: 731 2185
BanI G'GyrC_C
Cuts at: 0 31 679 872 1083 2916
Size: 31 648 193 211 1833
Fragments arranged by size:
1833 648 211 193 31
BanII G_rGCy'C
Cuts at: 0 42 131 403 953 2314 2916
Size: 42 89 272 550 1361 602
Fragments arranged by size:
1361 602 550 272 89 42
BbeI G_GCGC'C
Cuts at: 0 876 1087 2916
Size: 876 211 1829
Fragments arranged by size:
1829 876 211
BbsI GAAGACnn'nnnn_
Cuts at: 0 1662 1748 2007 2748 2916
Size: 1662 86 259 741 168
Fragments arranged by size:
1662 741 259 168 86
BbvCI CC'TCA_GC
Cuts at: 0 2062 2916
Size: 2062 854
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4 38 2916
Size: 4 34 2878
Fragments arranged by size:
2878 34 4
BfrBI ATG'CAT
Cuts at: 0 2839 2916
Size: 2839 77
BglI GCCn_nnn'nGGC
Cuts at: 0 1336 1438 1933 2916
Size: 1336 102 495 983
Fragments arranged by size:
1336 983 495 102
BglII A'GATC_T
Cuts at: 0 373 423 2630 2916
Size: 373 50 2207 286
Fragments arranged by size:
2207 373 286 50
BlpI GC'TnA_GC
Cuts at: 0 147 2140 2916
Size: 147 1993 776
Fragments arranged by size:
1993 776 147
Bme1580I G_kGCm'C
Cuts at: 0 42 776 953 2314 2916
Size: 42 734 177 1361 602
Fragments arranged by size:
1361 734 602 177 42
BmgBI CAC'GTC
Cuts at: 0 72 2010 2543 2916
Size: 72 1938 533 373
Fragments arranged by size:
1938 533 373 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 406 882 2118 2916
Size: 406 476 1236 798
Fragments arranged by size:
1236 798 476 406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 757 850 2191 2728 2791 2916
Size: 415 342 93 1341 537 63 125
Fragments arranged by size:
1341 537 415 342 125 93 63
Bpu10I CC'TnA_GC
Cuts at: 0 1987 2062 2678 2916
Size: 1987 75 616 238
Fragments arranged by size:
1987 616 238 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1336 2220 2916
Size: 1336 884 696
Fragments arranged by size:
1336 884 696
BsaI GGTCTCn'nnnn_
Cuts at: 0 1126 1144 2534 2916
Size: 1126 18 1390 382
Fragments arranged by size:
1390 1126 382 18
BsaAI yAC'GTr
Cuts at: 0 771 2916
Size: 771 2145
BsaHI Gr'CG_yC
Cuts at: 0 873 1084 2324 2916
Size: 873 211 1240 592
Fragments arranged by size:
1240 873 592 211
BsaWI w'CCGG_w
Cuts at: 0 698 2654 2916
Size: 698 1956 262
Fragments arranged by size:
1956 698 262
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 207 237 511 541 1417 1447 1558 1588
Size: 207 30 274 30 876 30 111 30
Cuts at: 1588 2916
Size: 1328
Fragments arranged by size:
1328 876 274 207 111 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 215 535 847 1474 1477 1570 1573 1755
Size: 215 320 312 627 3 93 3 182
Cuts at: 1755 2170 2916
Size: 415 746
Fragments arranged by size:
746 627 415 320 312 215 182 93
3 3
BseYI C'CCAG_C
Cuts at: 0 87 837 857 2916
Size: 87 750 20 2059
Fragments arranged by size:
2059 750 87 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1120 1397 1674 1700 2386 2916
Size: 751 369 277 277 26 686 530
Fragments arranged by size:
751 686 530 369 277 277 26
BsiEI CG_ry'CG
Cuts at: 0 80 2916
Size: 80 2836
BsiHKAI G_wGCw'C
Cuts at: 0 17 761 2146 2916
Size: 17 744 1385 770
Fragments arranged by size:
1385 770 744 17
BsmI GAATG_Cn'
Cuts at: 0 232 2916
Size: 232 2684
Bsp1286I G_dGCh'C
Cuts at: 0 17 42 131 403 761 776 953 2146
Size: 17 25 89 272 358 15 177 1193
Cuts at: 2146 2314 2916
Size: 168 602
Fragments arranged by size:
1193 602 358 272 177 168 89 25
17 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1458 1530 2742 2916
Size: 1458 72 1212 174
Fragments arranged by size:
1458 1212 174 72
BsrBI CCG'CTC
Cuts at: 0 1275 1641 2916
Size: 1275 366 1275
Fragments arranged by size:
1275 1275 366
BsrDI GCAATG_nn'
Cuts at: 0 53 640 1427 1821 2171 2916
Size: 53 587 787 394 350 745
Fragments arranged by size:
787 745 587 394 350 53
BsrFI r'CCGG_y
Cuts at: 0 698 2916
Size: 698 2218
BssHII G'CGCG_C
Cuts at: 0 1964 2916
Size: 1964 952
BssSI C'ACGA_G
Cuts at: 0 1552 2731 2916
Size: 1552 1179 185
Fragments arranged by size:
1552 1179 185
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1473 2916
Size: 1473 1443
BstEII G'GTnAC_C
Cuts at: 0 718 1705 2916
Size: 718 987 1211
Fragments arranged by size:
1211 987 718
BstXI CCAn_nnnn'nTGG
Cuts at: 0 72 1859 2916
Size: 72 1787 1057
Fragments arranged by size:
1787 1057 72
BstYI r'GATC_y
Cuts at: 0 373 423 731 2339 2630 2916
Size: 373 50 308 1608 291 286
Fragments arranged by size:
1608 373 308 291 286 50
Bsu36I CC'TnA_GG
Cuts at: 0 227 2510 2916
Size: 227 2283 406
Fragments arranged by size:
2283 406 227
BtgI C'CryG_G
Cuts at: 0 27 1398 1937 2916
Size: 27 1371 539 979
Fragments arranged by size:
1371 979 539 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 158 1212 2916
Size: 158 1054 1704
Fragments arranged by size:
1704 1054 158
BtsI GCAGTG_nn'
Cuts at: 0 162 1095 1793 2719 2790 2916
Size: 162 933 698 926 71 126
Fragments arranged by size:
933 926 698 162 126 71
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1296 1331 2916
Size: 1296 35 1585
Fragments arranged by size:
1585 1296 35
DraI TTT'AAA
Cuts at: 0 960 2916
Size: 960 1956
EaeI y'GGCC_r
Cuts at: 0 77 1385 1907 2411 2916
Size: 77 1308 522 504 505
Fragments arranged by size:
1308 522 505 504 77
EagI C'GGCC_G
Cuts at: 0 77 2916
Size: 77 2839
EarI CTCTTCn'nnn_
Cuts at: 0 1142 1835 2383 2793 2916
Size: 1142 693 548 410 123
Fragments arranged by size:
1142 693 548 410 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 757 850 1859 2191 2728 2791 2878
Size: 415 342 93 1009 332 537 63 87
Cuts at: 2878 2916
Size: 38
Fragments arranged by size:
1009 537 415 342 332 93 87 63
38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 799 2888 2916
Size: 799 2089 28
Fragments arranged by size:
2089 799 28
EcoO109I rG'GnC_Cy
Cuts at: 0 38 949 950 1869 2298 2766 2916
Size: 38 911 1 919 429 468 150
Fragments arranged by size:
919 911 468 429 150 38 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 337 2916
Size: 337 2579
FspI TGC'GCA
Cuts at: 0 2782 2916
Size: 2782 134
HaeII r_GCGC'y
Cuts at: 0 876 1087 2243 2678 2916
Size: 876 211 1156 435 238
Fragments arranged by size:
1156 876 435 238 211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 511 543 1033 1065 1264 1296 1747 1779
Size: 511 32 490 32 199 32 451 32
Cuts at: 1779 2916
Size: 1137
Fragments arranged by size:
1137 511 490 451 199 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2607 2916
Size: 2607 309
KasI G'GCGC_C
Cuts at: 0 872 1083 2916
Size: 872 211 1833
Fragments arranged by size:
1833 872 211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 2802 2916
Size: 730 2072 114
Fragments arranged by size:
2072 730 114
MscI TGG'CCA
Cuts at: 0 1387 1909 2916
Size: 1387 522 1007
Fragments arranged by size:
1387 1007 522
MslI CAynn'nnrTG
Cuts at: 0 70 499 1498 2452 2698 2735 2916
Size: 70 429 999 954 246 37 181
Fragments arranged by size:
999 954 429 246 181 70 37
MspA1I CmG'CkG
Cuts at: 0 54 1356 1688 1881 2316 2666 2856 2916
Size: 54 1302 332 193 435 350 190 60
Fragments arranged by size:
1302 435 350 332 193 190 60 54
NarI GG'CG_CC
Cuts at: 0 873 1084 2916
Size: 873 211 1832
Fragments arranged by size:
1832 873 211
NcoI C'CATG_G
Cuts at: 0 27 1398 1937 2916
Size: 27 1371 539 979
Fragments arranged by size:
1371 979 539 27
NruI TCG'CGA
Cuts at: 0 2279 2916
Size: 2279 637
NsiI A_TGCA'T
Cuts at: 0 2841 2916
Size: 2841 75
NspI r_CATG'y
Cuts at: 0 87 250 696 1802 2256 2355 2587 2781
Size: 87 163 446 1106 454 99 232 194
Cuts at: 2781 2916
Size: 135
Fragments arranged by size:
1106 454 446 232 194 163 135 99
87
PflMI CCAn_nnn'nTGG
Cuts at: 0 2769 2916
Size: 2769 147
PfoI T'CCnGG_A
Cuts at: 0 431 734 2916
Size: 431 303 2182
Fragments arranged by size:
2182 431 303
PmlI CAC'GTG
Cuts at: 0 771 2916
Size: 771 2145
PpuMI rG'GwC_Cy
Cuts at: 0 2298 2916
Size: 2298 618
PshAI GACnn'nnGTC
Cuts at: 0 340 2916
Size: 340 2576
PspOMI G'GGCC_C
Cuts at: 0 38 949 2310 2916
Size: 38 911 1361 606
Fragments arranged by size:
1361 911 606 38
PstI C_TGCA'G
Cuts at: 0 474 1541 1634 1997 2855 2916
Size: 474 1067 93 363 858 61
Fragments arranged by size:
1067 858 474 363 93 61
PvuII CAG'CTG
Cuts at: 0 1356 1688 2666 2856 2916
Size: 1356 332 978 190 60
Fragments arranged by size:
1356 978 332 190 60
SalI G'TCGA_C
Cuts at: 0 2605 2916
Size: 2605 311
SanDI GG'GwC_CC
Cuts at: 0 2298 2916
Size: 2298 618
SbfI CC_TGCA'GG
Cuts at: 0 1541 2916
Size: 1541 1375
SfcI C'TryA_G
Cuts at: 0 470 1537 1630 1993 2851 2916
Size: 470 1067 93 363 858 65
Fragments arranged by size:
1067 858 470 363 93 65
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1933 2916
Size: 1933 983
SfoI GGC'GCC
Cuts at: 0 874 1085 2916
Size: 874 211 1831
Fragments arranged by size:
1831 874 211
SmaI CCC'GGG
Cuts at: 0 2335 2916
Size: 2335 581
SmlI C'TyrA_G
Cuts at: 0 1315 2199 2916
Size: 1315 884 717
Fragments arranged by size:
1315 884 717
SphI G_CATG'C
Cuts at: 0 250 1802 2355 2587 2781 2916
Size: 250 1552 553 232 194 135
Fragments arranged by size:
1552 553 250 232 194 135
StuI AGG'CCT
Cuts at: 0 1441 2883 2916
Size: 1441 1442 33
Fragments arranged by size:
1442 1441 33
StyI C'CwwG_G
Cuts at: 0 27 1101 1217 1292 1398 1436 1937 2184
Size: 27 1074 116 75 106 38 501 247
Cuts at: 2184 2916
Size: 732
Fragments arranged by size:
1074 732 501 247 116 106 75 38
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 901 943 2516 2776 2916
Size: 901 42 1573 260 140
Fragments arranged by size:
1573 901 260 140 42
TatI w'GTAC_w
Cuts at: 0 941 2916
Size: 941 1975
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 207 239 1558 1590 2916
Size: 207 32 1319 32 1326
Fragments arranged by size:
1326 1319 207 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 552 565 1069 2011 2916
Size: 552 13 504 942 905
Fragments arranged by size:
942 905 552 504 13
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 1935 2115 2916
Size: 69 1866 180 801
Fragments arranged by size:
1866 801 180 69
XmaI C'CCGG_G
Cuts at: 0 2333 2916
Size: 2333 583
XmnI GAAnn'nnTTC
Cuts at: 0 998 2863 2916
Size: 998 1865 53
Fragments arranged by size:
1865 998 53
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AgeI AlfI AloI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NarI NcoI NruI NsiI NspI PflMI PfoI
PmlI PpuMI PshAI PspOMI PstI PvuII SalI SanDI
SbfI SfcI SfiI SfoI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AflII AflIII AhdI AleI ApaLI AscI
AseI AsiSI AvrII BaeI BciVI BclI BmtI BplI
BsaBI BsiWI BsmBI BspEI BspHI BsrGI BstBI BstZ17I
ClaI DraIII DrdI EciI EcoICRI EcoRI EcoRV FseI
FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI
NgoMIV NheI NotI PacI PasI PciI PmeI PpiI
PsiI PspXI PsrI PvuI RsrII SacI SacII SapI
ScaI SexAI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI ZraI