Restriction map for
pFN21AB8531
(Linear) (Six-base) MAPSORT of: KIBB8531.seq Check: 5816 from: 1 to: 2889
KIBB8531.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AclI AA'CG_TT
Cuts at: 0 1818 2889
Size: 1818 1071
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 83 749 1366 1594 2362 2889
Size: 27 56 666 617 228 768 527
Fragments arranged by size:
768 666 617 527 228 56 27
AfeI AGC'GCT
Cuts at: 0 2475 2889
Size: 2475 414
AflII C'TTAA_G
Cuts at: 0 1618 2889
Size: 1618 1271
AflIII A'CryG_T
Cuts at: 0 570 2889
Size: 570 2319
AleI CACnn'nnGTG
Cuts at: 0 115 2530 2810 2889
Size: 115 2415 280 79
Fragments arranged by size:
2415 280 115 79
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2096 2128 2889
Size: 2096 32 761
Fragments arranged by size:
2096 761 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2418 2889
Size: 2418 471
ApaLI G'TGCA_C
Cuts at: 0 501 2533 2889
Size: 501 2032 356
Fragments arranged by size:
2032 501 356
ApoI r'AATT_y
Cuts at: 0 372 379 1233 2889
Size: 372 7 854 1656
Fragments arranged by size:
1656 854 372 7
AseI AT'TA_AT
Cuts at: 0 629 2889
Size: 629 2260
AvaI C'yCGr_G
Cuts at: 0 2776 2889
Size: 2776 113
BanI G'GyrC_C
Cuts at: 0 1119 2889
Size: 1119 1770
BanII G_rGCy'C
Cuts at: 0 1094 2889
Size: 1094 1795
BbsI GAAGACnn'nnnn_
Cuts at: 0 113 277 476 1354 1535 2889
Size: 113 164 199 878 181 1354
Fragments arranged by size:
1354 878 199 181 164 113
BbvCI CC'TCA_GC
Cuts at: 0 806 2889
Size: 806 2083
BciVI GTATCCnnnnn_n'
Cuts at: 0 534 2889
Size: 534 2355
BclI T'GATC_A
Cuts at: 0 1506 2889
Size: 1506 1383
BfrBI ATG'CAT
Cuts at: 0 139 2662 2889
Size: 139 2523 227
Fragments arranged by size:
2523 227 139
BglII A'GATC_T
Cuts at: 0 2562 2889
Size: 2562 327
BlpI GC'TnA_GC
Cuts at: 0 1415 1604 2889
Size: 1415 189 1285
Fragments arranged by size:
1415 1285 189
Bme1580I G_kGCm'C
Cuts at: 0 505 2537 2889
Size: 505 2032 352
Fragments arranged by size:
2032 505 352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 2889
Size: 743 2146
Bpu10I CC'TnA_GC
Cuts at: 0 806 2889
Size: 806 2083
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 2889
Size: 155 2734
BsaAI yAC'GTr
Cuts at: 0 2551 2889
Size: 2551 338
BsaWI w'CCGG_w
Cuts at: 0 2464 2889
Size: 2464 425
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 600 630 2889
Size: 600 30 2259
Fragments arranged by size:
2259 600 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1384 2840 2888 2889
Size: 1384 1456 48 1
Fragments arranged by size:
1456 1384 48 1
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2735 2889
Size: 2735 154
BsiEI CG_ry'CG
Cuts at: 0 116 2889
Size: 116 2773
BsiHKAI G_wGCw'C
Cuts at: 0 505 1094 2028 2060 2537 2889
Size: 505 589 934 32 477 352
Fragments arranged by size:
934 589 505 477 352 32
BsmI GAATG_Cn'
Cuts at: 0 2745 2889
Size: 2745 144
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1108 2889
Size: 1108 1781
Bsp1286I G_dGCh'C
Cuts at: 0 505 1094 2028 2060 2537 2889
Size: 505 589 934 32 477 352
Fragments arranged by size:
934 589 505 477 352 32
BspHI T'CATG_A
Cuts at: 0 297 465 2889
Size: 297 168 2424
Fragments arranged by size:
2424 297 168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2402 2889
Size: 2402 487
BssSI C'ACGA_G
Cuts at: 0 2666 2889
Size: 2666 223
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2758 2889
Size: 2758 131
BstYI r'GATC_y
Cuts at: 0 1055 2111 2562 2889
Size: 1055 1056 451 327
Fragments arranged by size:
1056 1055 451 327
Bsu36I CC'TnA_GG
Cuts at: 0 1730 2889
Size: 1730 1159
BtgI C'CryG_G
Cuts at: 0 72 362 1808 2889
Size: 72 290 1446 1081
Fragments arranged by size:
1446 1081 290 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 72 2889
Size: 72 2817
BtsI GCAGTG_nn'
Cuts at: 0 2867 2889
Size: 2867 22
EaeI y'GGCC_r
Cuts at: 0 11 954 2732 2889
Size: 11 943 1778 157
Fragments arranged by size:
1778 943 157 11
EarI CTCTTCn'nnn_
Cuts at: 0 1748 2351 2733 2889
Size: 1748 603 382 156
Fragments arranged by size:
1748 603 382 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 2889
Size: 19 2870
EcoICRI GAG'CTC
Cuts at: 0 1092 2889
Size: 1092 1797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 83 743 749 1366 1594 2362 2889
Size: 27 56 660 6 617 228 768 527
Fragments arranged by size:
768 660 617 527 228 56 27 6
EcoO109I rG'GnC_Cy
Cuts at: 0 429 1479 2415 2889
Size: 429 1050 936 474
Fragments arranged by size:
1050 936 474 429
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 235 2076 2200 2889
Size: 235 1841 124 689
Fragments arranged by size:
1841 689 235 124
FspI TGC'GCA
Cuts at: 0 2398 2889
Size: 2398 491
HaeII r_GCGC'y
Cuts at: 0 2477 2889
Size: 2477 412
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1498 1530 2889
Size: 1498 32 1359
Fragments arranged by size:
1498 1359 32
HincII GTy'rAC
Cuts at: 0 1004 1330 2889
Size: 1004 326 1559
Fragments arranged by size:
1559 1004 326
HindIII A'AGCT_T
Cuts at: 0 1163 2624 2889
Size: 1163 1461 265
Fragments arranged by size:
1461 1163 265
HpaI GTT'AAC
Cuts at: 0 1004 2889
Size: 1004 1885
MfeI C'AATT_G
Cuts at: 0 1217 2889
Size: 1217 1672
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 1340 1958 2567 2824 2889
Size: 587 753 618 609 257 65
Fragments arranged by size:
753 618 609 587 257 65
MscI TGG'CCA
Cuts at: 0 956 2734 2889
Size: 956 1778 155
Fragments arranged by size:
1778 956 155
MslI CAynn'nnrTG
Cuts at: 0 28 115 2530 2810 2889
Size: 28 87 2415 280 79
Fragments arranged by size:
2415 280 87 79 28
MspA1I CmG'CkG
Cuts at: 0 74 1654 2570 2611 2889
Size: 74 1580 916 41 278
Fragments arranged by size:
1580 916 278 74 41
NcoI C'CATG_G
Cuts at: 0 362 1808 2889
Size: 362 1446 1081
Fragments arranged by size:
1446 1081 362
NdeI CA'TA_TG
Cuts at: 0 249 819 2889
Size: 249 570 2070
Fragments arranged by size:
2070 570 249
NsiI A_TGCA'T
Cuts at: 0 141 2664 2889
Size: 141 2523 225
Fragments arranged by size:
2523 225 141
NspI r_CATG'y
Cuts at: 0 574 1895 2889
Size: 574 1321 994
Fragments arranged by size:
1321 994 574
PciI A'CATG_T
Cuts at: 0 570 2889
Size: 570 2319
PflMI CCAn_nnn'nTGG
Cuts at: 0 2046 2856 2889
Size: 2046 810 33
Fragments arranged by size:
2046 810 33
PpuMI rG'GwC_Cy
Cuts at: 0 429 2415 2889
Size: 429 1986 474
Fragments arranged by size:
1986 474 429
PstI C_TGCA'G
Cuts at: 0 1011 2889
Size: 1011 1878
PvuII CAG'CTG
Cuts at: 0 1654 2611 2889
Size: 1654 957 278
Fragments arranged by size:
1654 957 278
SacI G_AGCT'C
Cuts at: 0 1094 2889
Size: 1094 1795
SacII CC_GC'GG
Cuts at: 0 75 2889
Size: 75 2814
SfcI C'TryA_G
Cuts at: 0 879 1007 2606 2889
Size: 879 128 1599 283
Fragments arranged by size:
1599 879 283 128
SmaI CCC'GGG
Cuts at: 0 2778 2889
Size: 2778 111
SmlI C'TyrA_G
Cuts at: 0 170 1618 2889
Size: 170 1448 1271
Fragments arranged by size:
1448 1271 170
SpeI A'CTAG_T
Cuts at: 0 2010 2889
Size: 2010 879
SspI AAT'ATT
Cuts at: 0 242 2889
Size: 242 2647
StyI C'CwwG_G
Cuts at: 0 362 1808 2889
Size: 362 1446 1081
Fragments arranged by size:
1446 1081 362
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2822 2889
Size: 2822 67
TatI w'GTAC_w
Cuts at: 0 83 216 814 2889
Size: 83 133 598 2075
Fragments arranged by size:
2075 598 133 83
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1688 1720 2889
Size: 1688 32 1169
Fragments arranged by size:
1688 1169 32
Tth111I GACn'n_nGTC
Cuts at: 0 112 2889
Size: 112 2777
XmaI C'CCGG_G
Cuts at: 0 2776 2889
Size: 2776 113
XmnI GAAnn'nnTTC
Cuts at: 0 1946 2889
Size: 1946 943
Enzymes that do cut:
AclI AcuI AfeI AflII AflIII AleI AloI AlwNI
ApaLI ApoI AseI AvaI BanI BanII BbsI BbvCI
BciVI BclI BfrBI BglII BlpI Bme1580I BpmI Bpu10I
BpuEI BsaAI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BssSI BstAPI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I FalI FspI HaeII Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NcoI NdeI
NsiI NspI PciI PflMI PpuMI PstI PvuII SacI
SacII SfcI SmaI SmlI SpeI SspI StyI TaqII
TatI TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AgeI AhdI AlfI ApaI
AscI AsiSI AvrII BaeI BamHI BbeI BcgI BglI
BmgBI BmrI BmtI BplI BsaI BsaBI BsaHI BseYI
BsiWI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BstBI
BstEII BstXI BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EcoNI EcoRI EcoRV FseI FspAI KasI KpnI
MluI NaeI NarI NgoMIV NheI NotI NruI PacI
PasI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SphI
SrfI StuI SwaI XbaI XcmI XhoI ZraI