Restriction map for
pFN21AB8524
(Linear) (Six-base) MAPSORT of: KIBB8524.seq Check: 400 from: 1 to: 2511
KIBB8524.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 165 2511
Size: 165 2346
AccI GT'mk_AC
Cuts at: 0 260 2511
Size: 260 2251
Acc65I G'GTAC_C
Cuts at: 0 1314 2511
Size: 1314 1197
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1175 1357 1505 2511
Size: 133 1042 182 148 1006
Fragments arranged by size:
1042 1006 182 148 133
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 838 870 2511
Size: 838 32 1641
Fragments arranged by size:
1641 838 32
AlwNI CAG_nnn'CTG
Cuts at: 0 63 1335 2248 2511
Size: 63 1272 913 263
Fragments arranged by size:
1272 913 263 63
ApaI G_GGCC'C
Cuts at: 0 917 2511
Size: 917 1594
ApoI r'AATT_y
Cuts at: 0 1389 2511
Size: 1389 1122
AvaI C'yCGr_G
Cuts at: 0 1078 2214 2511
Size: 1078 1136 297
Fragments arranged by size:
1136 1078 297
BamHI G'GATC_C
Cuts at: 0 164 2511
Size: 164 2347
BanI G'GyrC_C
Cuts at: 0 13 616 822 1314 2511
Size: 13 603 206 492 1197
Fragments arranged by size:
1197 603 492 206 13
BanII G_rGCy'C
Cuts at: 0 477 797 880 917 1733 1983 2511
Size: 477 320 83 37 816 250 528
Fragments arranged by size:
816 528 477 320 250 83 37
BbeI G_GCGC'C
Cuts at: 0 17 2511
Size: 17 2494
BbsI GAAGACnn'nnnn_
Cuts at: 0 1232 1280 2464 2511
Size: 1232 48 1184 47
Fragments arranged by size:
1232 1184 48 47
BbvCI CC'TCA_GC
Cuts at: 0 901 2125 2511
Size: 901 1224 386
Fragments arranged by size:
1224 901 386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2341 2375 2511
Size: 2341 34 136
Fragments arranged by size:
2341 136 34
BglI GCCn_nnn'nGGC
Cuts at: 0 140 614 712 2511
Size: 140 474 98 1799
Fragments arranged by size:
1799 474 140 98
Bme1580I G_kGCm'C
Cuts at: 0 527 827 917 2415 2511
Size: 527 300 90 1498 96
Fragments arranged by size:
1498 527 300 96 90
BmgBI CAC'GTC
Cuts at: 0 1851 2511
Size: 1851 660
BmrI ACTGGGnnnn_n'
Cuts at: 0 911 2511
Size: 911 1600
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 446 599 2511
Size: 446 153 1912
Fragments arranged by size:
1912 446 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 463 1078 1312 1741 2511
Size: 66 397 615 234 429 770
Fragments arranged by size:
770 615 429 397 234 66
Bpu10I CC'TnA_GC
Cuts at: 0 901 2125 2511
Size: 901 1224 386
Fragments arranged by size:
1224 901 386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 1177 1275 1396 2511
Size: 441 736 98 121 1115
Fragments arranged by size:
1115 736 441 121 98
BsaAI yAC'GTr
Cuts at: 0 725 2511
Size: 725 1786
BsaHI Gr'CG_yC
Cuts at: 0 14 2511
Size: 14 2497
BsaWI w'CCGG_w
Cuts at: 0 38 1769 2511
Size: 38 1731 742
Fragments arranged by size:
1731 742 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1882 1912 2511
Size: 1882 30 599
Fragments arranged by size:
1882 599 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 450 957 2511
Size: 450 507 1554
Fragments arranged by size:
1554 507 450
BseYI C'CCAG_C
Cuts at: 0 18 365 520 910 1025 1029 2511
Size: 18 347 155 390 115 4 1482
Fragments arranged by size:
1482 390 347 155 115 18 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 517 2366 2511
Size: 199 318 1849 145
Fragments arranged by size:
1849 318 199 145
BsiEI CG_ry'CG
Cuts at: 0 135 974 2000 2511
Size: 135 839 1026 511
Fragments arranged by size:
1026 839 511 135
BsiHKAI G_wGCw'C
Cuts at: 0 880 1733 1983 2511
Size: 880 853 250 528
Fragments arranged by size:
880 853 528 250
BsmI GAATG_Cn'
Cuts at: 0 1041 1694 1902 2298 2511
Size: 1041 653 208 396 213
Fragments arranged by size:
1041 653 396 213 208
Bsp1286I G_dGCh'C
Cuts at: 0 477 527 797 827 880 917 1733 1983
Size: 477 50 270 30 53 37 816 250
Cuts at: 1983 2415 2511
Size: 432 96
Fragments arranged by size:
816 477 432 270 250 96 53 50
37 30
BspEI T'CCGG_A
Cuts at: 0 38 2511
Size: 38 2473
BspHI T'CATG_A
Cuts at: 0 1421 2511
Size: 1421 1090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 165 798 2511
Size: 165 633 1713
Fragments arranged by size:
1713 633 165
BsrDI GCAATG_nn'
Cuts at: 0 1435 1646 2511
Size: 1435 211 865
Fragments arranged by size:
1435 865 211
BssSI C'ACGA_G
Cuts at: 0 1135 2511
Size: 1135 1376
BstAPI GCAn_nnn'nTGC
Cuts at: 0 531 2353 2511
Size: 531 1822 158
Fragments arranged by size:
1822 531 158
BstEII G'GTnAC_C
Cuts at: 0 2089 2511
Size: 2089 422
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1183 1353 1887 2511
Size: 1183 170 534 624
Fragments arranged by size:
1183 624 534 170
BstYI r'GATC_y
Cuts at: 0 59 164 2511
Size: 59 105 2347
Fragments arranged by size:
2347 105 59
Bsu36I CC'TnA_GG
Cuts at: 0 1974 2511
Size: 1974 537
BtgI C'CryG_G
Cuts at: 0 135 212 404 926 1676 2511
Size: 135 77 192 522 750 835
Fragments arranged by size:
835 750 522 192 135 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1606 2366 2511
Size: 1606 760 145
Fragments arranged by size:
1606 760 145
BtsI GCAGTG_nn'
Cuts at: 0 249 1073 2511
Size: 249 824 1438
Fragments arranged by size:
1438 824 249
DrdI GACnn_nn'nnGTC
Cuts at: 0 1624 2511
Size: 1624 887
EaeI y'GGCC_r
Cuts at: 0 132 141 963 971 1997 2336 2511
Size: 132 9 822 8 1026 339 175
Fragments arranged by size:
1026 822 339 175 132 9 8
EagI C'GGCC_G
Cuts at: 0 132 971 1997 2511
Size: 132 839 1026 514
Fragments arranged by size:
1026 839 514 132
EarI CTCTTCn'nnn_
Cuts at: 0 366 1159 2511
Size: 366 793 1352
Fragments arranged by size:
1352 793 366
EcoICRI GAG'CTC
Cuts at: 0 878 1731 1981 2511
Size: 878 853 250 530
Fragments arranged by size:
878 853 530 250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 133 463 1078 1175 1312 1357 1505
Size: 66 67 330 615 97 137 45 148
Cuts at: 1505 1741 2511
Size: 236 770
Fragments arranged by size:
770 615 330 236 148 137 97 67
66 45
EcoO109I rG'GnC_Cy
Cuts at: 0 416 860 1250 2511
Size: 416 444 390 1261
Fragments arranged by size:
1261 444 416 390
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1456 2511
Size: 1456 1055
HaeII r_GCGC'y
Cuts at: 0 17 94 636 1577 2511
Size: 17 77 542 941 934
Fragments arranged by size:
941 934 542 77 17
HincII GTy'rAC
Cuts at: 0 1173 2472 2511
Size: 1173 1299 39
Fragments arranged by size:
1299 1173 39
HindIII A'AGCT_T
Cuts at: 0 1195 2511
Size: 1195 1316
HpaI GTT'AAC
Cuts at: 0 1173 2511
Size: 1173 1338
KasI G'GCGC_C
Cuts at: 0 13 2511
Size: 13 2498
KpnI G_GTAC'C
Cuts at: 0 1318 2511
Size: 1318 1193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 2390 2511
Size: 273 2117 121
Fragments arranged by size:
2117 273 121
MscI TGG'CCA
Cuts at: 0 2338 2511
Size: 2338 173
MslI CAynn'nnrTG
Cuts at: 0 1048 2087 2511
Size: 1048 1039 424
Fragments arranged by size:
1048 1039 424
MspA1I CmG'CkG
Cuts at: 0 22 131 137 819 938 1029 1719 2511
Size: 22 109 6 682 119 91 690 792
Fragments arranged by size:
792 690 682 119 109 91 22 6
NarI GG'CG_CC
Cuts at: 0 14 2511
Size: 14 2497
NotI GC'GGCC_GC
Cuts at: 0 132 971 2511
Size: 132 839 1540
Fragments arranged by size:
1540 839 132
NspI r_CATG'y
Cuts at: 0 330 1095 2086 2511
Size: 330 765 991 425
Fragments arranged by size:
991 765 425 330
PfoI T'CCnGG_A
Cuts at: 0 167 1102 2137 2511
Size: 167 935 1035 374
Fragments arranged by size:
1035 935 374 167
PpuMI rG'GwC_Cy
Cuts at: 0 416 860 2511
Size: 416 444 1651
Fragments arranged by size:
1651 444 416
PshAI GACnn'nnGTC
Cuts at: 0 2038 2511
Size: 2038 473
PsiI TTA'TAA
Cuts at: 0 1915 2511
Size: 1915 596
PspOMI G'GGCC_C
Cuts at: 0 913 2511
Size: 913 1598
PspXI vC'TCGA_Gb
Cuts at: 0 1078 2511
Size: 1078 1433
PstI C_TGCA'G
Cuts at: 0 833 1119 1205 1988 2063 2156 2511
Size: 833 286 86 783 75 93 355
Fragments arranged by size:
833 783 355 286 93 86 75
PvuII CAG'CTG
Cuts at: 0 22 1029 1719 2511
Size: 22 1007 690 792
Fragments arranged by size:
1007 792 690 22
RsrII CG'GwC_CG
Cuts at: 0 41 2511
Size: 41 2470
SacI G_AGCT'C
Cuts at: 0 880 1733 1983 2511
Size: 880 853 250 528
Fragments arranged by size:
880 853 528 250
SacII CC_GC'GG
Cuts at: 0 138 2511
Size: 138 2373
ScaI AGT'ACT
Cuts at: 0 2220 2511
Size: 2220 291
SfcI C'TryA_G
Cuts at: 0 829 1115 1201 1984 2059 2152 2240 2511
Size: 829 286 86 783 75 93 88 271
Fragments arranged by size:
829 783 286 271 93 88 86 75
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 140 2511
Size: 140 2371
SfoI GGC'GCC
Cuts at: 0 15 2511
Size: 15 2496
SmlI C'TyrA_G
Cuts at: 0 420 1078 1192 1254 1411 2511
Size: 420 658 114 62 157 1100
Fragments arranged by size:
1100 658 420 157 114 62
SspI AAT'ATT
Cuts at: 0 1744 2511
Size: 1744 767
StyI C'CwwG_G
Cuts at: 0 1880 2511
Size: 1880 631
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1466 2511
Size: 1466 1045
TatI w'GTAC_w
Cuts at: 0 386 500 2218 2511
Size: 386 114 1718 293
Fragments arranged by size:
1718 386 293 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1882 1914 2511
Size: 1882 32 597
Fragments arranged by size:
1882 597 32
Tth111I GACn'n_nGTC
Cuts at: 0 2209 2511
Size: 2209 302
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 114 750 924 2050 2511
Size: 114 636 174 1126 461
Fragments arranged by size:
1126 636 461 174 114
XhoI C'TCGA_G
Cuts at: 0 1078 2511
Size: 1078 1433
XmnI GAAnn'nnTTC
Cuts at: 0 1229 2511
Size: 1229 1282
Enzymes that do cut:
AarI AccI Acc65I AcuI AloI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI
HaeII HincII HindIII HpaI KasI KpnI MmeI MscI
MslI MspA1I NarI NotI NspI PfoI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuII RsrII SacI SacII
ScaI SfcI SfiI SfoI SmlI SspI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BciVI
BclI BfrBI BglII BlpI BmtI BsaI BsaBI BsiWI
BsmBI BsrBI BsrFI BsrGI BssHII BstBI BstZ17I ClaI
CspCI DraI DraIII EciI EcoNI EcoRI EcoRV FseI
FspI FspAI Hin4I MfeI MluI NaeI NcoI NdeI
NgoMIV NheI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PsrI PvuI SalI SanDI SapI
SbfI SexAI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI XbaI XmaI ZraI