Restriction map for
pFN21AB8520
(Linear) (Six-base) MAPSORT of: KIBB8520.seq Check: 3689 from: 1 to: 2622
KIBB8520.orf
With 182 enzymes: SgfI *
May 26, 2011 13:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 924 1607 2622
Size: 924 683 1015
Fragments arranged by size:
1015 924 683
AfeI AGC'GCT
Cuts at: 0 1019 2260 2622
Size: 1019 1241 362
Fragments arranged by size:
1241 1019 362
AflII C'TTAA_G
Cuts at: 0 475 2622
Size: 475 2147
AflIII A'CryG_T
Cuts at: 0 1576 2085 2622
Size: 1576 509 537
Fragments arranged by size:
1576 537 509
AhdI GACnn_n'nnGTC
Cuts at: 0 21 2622
Size: 21 2601
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1892 1924 2622
Size: 1892 32 698
Fragments arranged by size:
1892 698 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1423 2501 2622
Size: 1423 1078 121
Fragments arranged by size:
1423 1078 121
ApaLI G'TGCA_C
Cuts at: 0 623 1194 2622
Size: 623 571 1428
Fragments arranged by size:
1428 623 571
ApoI r'AATT_y
Cuts at: 0 360 497 1209 1310 2185 2602 2622
Size: 360 137 712 101 875 417 20
Fragments arranged by size:
875 712 417 360 137 101 20
AvaI C'yCGr_G
Cuts at: 0 770 2622
Size: 770 1852
BamHI G'GATC_C
Cuts at: 0 1052 1828 2622
Size: 1052 776 794
Fragments arranged by size:
1052 794 776
BanI G'GyrC_C
Cuts at: 0 1336 2622
Size: 1336 1286
BanII G_rGCy'C
Cuts at: 0 116 134 1642 1759 1777 1857 2622
Size: 116 18 1508 117 18 80 765
Fragments arranged by size:
1508 765 117 116 80 18 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 20 1033 2622
Size: 20 1013 1589
Fragments arranged by size:
1589 1013 20
BbvCI CC'TCA_GC
Cuts at: 0 1671 2120 2622
Size: 1671 449 502
Fragments arranged by size:
1671 502 449
BciVI GTATCCnnnnn_n'
Cuts at: 0 786 2622
Size: 786 1836
BclI T'GATC_A
Cuts at: 0 818 1487 2622
Size: 818 669 1135
Fragments arranged by size:
1135 818 669
BglI GCCn_nnn'nGGC
Cuts at: 0 1367 2622
Size: 1367 1255
BlpI GC'TnA_GC
Cuts at: 0 1264 1811 2622
Size: 1264 547 811
Fragments arranged by size:
1264 811 547
Bme1580I G_kGCm'C
Cuts at: 0 627 1198 1480 2622
Size: 627 571 282 1142
Fragments arranged by size:
1142 627 571 282
BmgBI CAC'GTC
Cuts at: 0 1577 2351 2622
Size: 1577 774 271
Fragments arranged by size:
1577 774 271
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 581 2622
Size: 581 2041
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 1322 1867 2092 2398 2622
Size: 572 750 545 225 306 224
Fragments arranged by size:
750 572 545 306 225 224
Bpu10I CC'TnA_GC
Cuts at: 0 760 1671 2120 2622
Size: 760 911 449 502
Fragments arranged by size:
911 760 502 449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1743 2099 2622
Size: 1743 356 523
Fragments arranged by size:
1743 523 356
BsaI GGTCTCn'nnnn_
Cuts at: 0 2374 2622
Size: 2374 248
BsaHI Gr'CG_yC
Cuts at: 0 1870 2622
Size: 1870 752
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1892 1922 2622
Size: 1892 30 700
Fragments arranged by size:
1892 700 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 498 1108 1561 1728 2579 2600 2622
Size: 22 476 610 453 167 851 21 22
Fragments arranged by size:
851 610 476 453 167 22 22 21
BseYI C'CCAG_C
Cuts at: 0 429 1776 1878 2622
Size: 429 1347 102 744
Fragments arranged by size:
1347 744 429 102
BsiEI CG_ry'CG
Cuts at: 0 810 2622
Size: 810 1812
BsiHKAI G_wGCw'C
Cuts at: 0 134 627 1198 1759 2622
Size: 134 493 571 561 863
Fragments arranged by size:
863 571 561 493 134
BsmI GAATG_Cn'
Cuts at: 0 2537 2622
Size: 2537 85
BsmBI CGTCTCn'nnnn_
Cuts at: 0 197 2622
Size: 197 2425
Bsp1286I G_dGCh'C
Cuts at: 0 116 134 627 1198 1480 1642 1759 1777
Size: 116 18 493 571 282 162 117 18
Cuts at: 1777 1857 2622
Size: 80 765
Fragments arranged by size:
765 571 493 282 162 117 116 80
18 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1577 2392 2622
Size: 1577 815 230
Fragments arranged by size:
1577 815 230
BsrFI r'CCGG_y
Cuts at: 0 805 2622
Size: 805 1817
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1276 2351 2622
Size: 1276 1075 271
Fragments arranged by size:
1276 1075 271
BstXI CCAn_nnnn'nTGG
Cuts at: 0 561 735 2622
Size: 561 174 1887
Fragments arranged by size:
1887 561 174
BstYI r'GATC_y
Cuts at: 0 591 1052 1281 1828 2622
Size: 591 461 229 547 794
Fragments arranged by size:
794 591 547 461 229
Bsu36I CC'TnA_GG
Cuts at: 0 2060 2622
Size: 2060 562
BtgI C'CryG_G
Cuts at: 0 1819 2622
Size: 1819 803
BtsI GCAGTG_nn'
Cuts at: 0 37 2490 2622
Size: 37 2453 132
Fragments arranged by size:
2453 132 37
ClaI AT'CG_AT
Cuts at: 0 1112 2622
Size: 1112 1510
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 213 248 648 683 2622
Size: 213 35 400 35 1939
Fragments arranged by size:
1939 400 213 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 919 982 2622
Size: 919 63 1640
Fragments arranged by size:
1640 919 63
EaeI y'GGCC_r
Cuts at: 0 221 466 807 848 1859 2622
Size: 221 245 341 41 1011 763
Fragments arranged by size:
1011 763 341 245 221 41
EagI C'GGCC_G
Cuts at: 0 807 2622
Size: 807 1815
EarI CTCTTCn'nnn_
Cuts at: 0 164 332 1522 2422 2622
Size: 164 168 1190 900 200
Fragments arranged by size:
1190 900 200 168 164
EcoICRI GAG'CTC
Cuts at: 0 132 1757 2622
Size: 132 1625 865
Fragments arranged by size:
1625 865 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 924 1322 1607 1867 2092 2398 2622
Size: 572 352 398 285 260 225 306 224
Fragments arranged by size:
572 398 352 306 285 260 225 224
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2233 2622
Size: 2233 389
EcoO109I rG'GnC_Cy
Cuts at: 0 297 695 2055 2622
Size: 297 398 1360 567
Fragments arranged by size:
1360 567 398 297
EcoRI G'AATT_C
Cuts at: 0 497 1310 2622
Size: 497 813 1312
Fragments arranged by size:
1312 813 497
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 623 2622
Size: 623 1999
HaeII r_GCGC'y
Cuts at: 0 1021 2262 2622
Size: 1021 1241 360
Fragments arranged by size:
1241 1021 360
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 142 174 636 668 1273 1305
Size: 7 32 103 32 462 32 605 32
Cuts at: 1305 2622
Size: 1317
Fragments arranged by size:
1317 605 462 103 32 32 32 32
7
HincII GTy'rAC
Cuts at: 0 284 509 2622
Size: 284 225 2113
Fragments arranged by size:
2113 284 225
MfeI C'AATT_G
Cuts at: 0 1412 2622
Size: 1412 1210
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1685 2622
Size: 1685 937
MscI TGG'CCA
Cuts at: 0 223 850 2622
Size: 223 627 1772
Fragments arranged by size:
1772 627 223
MslI CAynn'nnrTG
Cuts at: 0 733 787 2414 2420 2622
Size: 733 54 1627 6 202
Fragments arranged by size:
1627 733 202 54 6
MspA1I CmG'CkG
Cuts at: 0 1423 1698 1780 2622
Size: 1423 275 82 842
Fragments arranged by size:
1423 842 275 82
NdeI CA'TA_TG
Cuts at: 0 946 2622
Size: 946 1676
NspI r_CATG'y
Cuts at: 0 515 2345 2599 2622
Size: 515 1830 254 23
Fragments arranged by size:
1830 515 254 23
PfoI T'CCnGG_A
Cuts at: 0 1493 2069 2622
Size: 1493 576 553
Fragments arranged by size:
1493 576 553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1892 1924 2622
Size: 1892 32 698
Fragments arranged by size:
1892 698 32
PpuMI rG'GwC_Cy
Cuts at: 0 297 695 2055 2622
Size: 297 398 1360 567
Fragments arranged by size:
1360 567 398 297
PshAI GACnn'nnGTC
Cuts at: 0 2386 2622
Size: 2386 236
PspXI vC'TCGA_Gb
Cuts at: 0 770 2622
Size: 770 1852
PstI C_TGCA'G
Cuts at: 0 1289 1754 2497 2622
Size: 1289 465 743 125
Fragments arranged by size:
1289 743 465 125
PvuII CAG'CTG
Cuts at: 0 1423 1780 2622
Size: 1423 357 842
Fragments arranged by size:
1423 842 357
SacI G_AGCT'C
Cuts at: 0 134 1759 2622
Size: 134 1625 863
Fragments arranged by size:
1625 863 134
SanDI GG'GwC_CC
Cuts at: 0 695 2622
Size: 695 1927
SapI GCTCTTCn'nnn_
Cuts at: 0 1522 2622
Size: 1522 1100
ScaI AGT'ACT
Cuts at: 0 1228 2622
Size: 1228 1394
SfcI C'TryA_G
Cuts at: 0 640 1285 1750 2493 2622
Size: 640 645 465 743 129
Fragments arranged by size:
743 645 640 465 129
SmlI C'TyrA_G
Cuts at: 0 475 770 1758 2114 2622
Size: 475 295 988 356 508
Fragments arranged by size:
988 508 475 356 295
StuI AGG'CCT
Cuts at: 0 382 2622
Size: 382 2240
StyI C'CwwG_G
Cuts at: 0 62 1079 2468 2622
Size: 62 1017 1389 154
Fragments arranged by size:
1389 1017 154 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 867 1129 2622
Size: 867 262 1493
Fragments arranged by size:
1493 867 262
TatI w'GTAC_w
Cuts at: 0 1226 1253 2622
Size: 1226 27 1369
Fragments arranged by size:
1369 1226 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 606 638 2622
Size: 606 32 1984
Fragments arranged by size:
1984 606 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 858 2416 2622
Size: 858 1558 206
Fragments arranged by size:
1558 858 206
XhoI C'TCGA_G
Cuts at: 0 770 2622
Size: 770 1852
XmnI GAAnn'nnTTC
Cuts at: 0 266 2622
Size: 266 2356
Enzymes that do cut:
AcuI AfeI AflII AflIII AhdI AloI AlwNI ApaLI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BciVI
BclI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrFI BstAPI BstXI BstYI
Bsu36I BtgI BtsI ClaI CspCI DraIII EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII MfeI MmeI MscI MslI MspA1I NdeI
NspI PfoI PpiI PpuMI PshAI PspXI PstI PvuII
SacI SanDI SapI ScaI SfcI SmlI StuI StyI
TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AleI AlfI
ApaI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BfrBI BglII BmrI BmtI BsaAI BsaBI BsaWI BsgI
BsiWI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstZ17I BtgZI DraI DrdI EciI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MluI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PsiI PspOMI
PsrI PvuI RsrII SacII SalI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XmaI ZraI