Restriction map for
pFN21AB8519
(Linear) (Six-base) MAPSORT of: KIBB8519.seq Check: 7599 from: 1 to: 2859
KIBB8519.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2206 2859
Size: 2206 653
AatII G_ACGT'C
Cuts at: 0 485 2859
Size: 485 2374
AccI GT'mk_AC
Cuts at: 0 125 1750 2859
Size: 125 1625 1109
Fragments arranged by size:
1625 1109 125
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 2054 2761 2859
Size: 377 1677 707 98
Fragments arranged by size:
1677 707 377 98
AflII C'TTAA_G
Cuts at: 0 55 541 1393 2665 2859
Size: 55 486 852 1272 194
Fragments arranged by size:
1272 852 486 194 55
AflIII A'CryG_T
Cuts at: 0 1809 2859
Size: 1809 1050
AhdI GACnn_n'nnGTC
Cuts at: 0 481 2859
Size: 481 2378
ApoI r'AATT_y
Cuts at: 0 61 110 980 1083 1201 2859
Size: 61 49 870 103 118 1658
Fragments arranged by size:
1658 870 118 103 61 49
AseI AT'TA_AT
Cuts at: 0 2006 2859
Size: 2006 853
AvaI C'yCGr_G
Cuts at: 0 2645 2859
Size: 2645 214
AvrII C'CTAG_G
Cuts at: 0 1545 2859
Size: 1545 1314
BamHI G'GATC_C
Cuts at: 0 838 2859
Size: 838 2021
BanI G'GyrC_C
Cuts at: 0 814 2859
Size: 814 2045
BanII G_rGCy'C
Cuts at: 0 1567 2859
Size: 1567 1292
BbsI GAAGACnn'nnnn_
Cuts at: 0 480 722 2762 2859
Size: 480 242 2040 97
Fragments arranged by size:
2040 480 242 97
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1395 1429 2859
Size: 1395 34 1430
Fragments arranged by size:
1430 1395 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1281 2739 2859
Size: 1281 1458 120
Fragments arranged by size:
1458 1281 120
BclI T'GATC_A
Cuts at: 0 2 315 429 2859
Size: 2 313 114 2430
Fragments arranged by size:
2430 313 114 2
BfrBI ATG'CAT
Cuts at: 0 311 2859
Size: 311 2548
BglI GCCn_nnn'nGGC
Cuts at: 0 187 2859
Size: 187 2672
Bme1580I G_kGCm'C
Cuts at: 0 1900 2061 2859
Size: 1900 161 798
Fragments arranged by size:
1900 798 161
BmgBI CAC'GTC
Cuts at: 0 1468 2859
Size: 1468 1391
BmrI ACTGGGnnnn_n'
Cuts at: 0 1203 2264 2295 2859
Size: 1203 1061 31 564
Fragments arranged by size:
1203 1061 564 31
BmtI G_CTAG'C
Cuts at: 0 770 2859
Size: 770 2089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 523 1280 2859
Size: 484 39 757 1579
Fragments arranged by size:
1579 757 484 39
Bpu10I CC'TnA_GC
Cuts at: 0 1577 2612 2859
Size: 1577 1035 247
Fragments arranged by size:
1577 1035 247
BsaI GGTCTCn'nnnn_
Cuts at: 0 1330 2680 2859
Size: 1330 1350 179
Fragments arranged by size:
1350 1330 179
BsaAI yAC'GTr
Cuts at: 0 2046 2859
Size: 2046 813
BsaHI Gr'CG_yC
Cuts at: 0 482 1800 2285 2859
Size: 482 1318 485 574
Fragments arranged by size:
1318 574 485 482
BsaWI w'CCGG_w
Cuts at: 0 161 2859
Size: 161 2698
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 2563 2566 2779 2821 2824 2859
Size: 238 2325 3 213 42 3 35
Fragments arranged by size:
2325 238 213 42 35 3 3
BseYI C'CCAG_C
Cuts at: 0 2640 2660 2859
Size: 2640 20 199
Fragments arranged by size:
2640 199 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1448 2859
Size: 1448 1411
BsiEI CG_ry'CG
Cuts at: 0 14 2859
Size: 14 2845
BsmI GAATG_Cn'
Cuts at: 0 87 2859
Size: 87 2772
Bsp1286I G_dGCh'C
Cuts at: 0 1567 1900 2061 2859
Size: 1567 333 161 798
Fragments arranged by size:
1567 798 333 161
BspEI T'CCGG_A
Cuts at: 0 161 2859
Size: 161 2698
BspHI T'CATG_A
Cuts at: 0 1049 1242 2081 2859
Size: 1049 193 839 778
Fragments arranged by size:
1049 839 778 193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2206 2859
Size: 2206 653
BsrDI GCAATG_nn'
Cuts at: 0 455 1413 1964 2859
Size: 455 958 551 895
Fragments arranged by size:
958 895 551 455
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2440 2859
Size: 2440 419
BstBI TT'CG_AA
Cuts at: 0 804 1734 2859
Size: 804 930 1125
Fragments arranged by size:
1125 930 804
BstYI r'GATC_y
Cuts at: 0 838 2859
Size: 838 2021
BstZ17I GTA'TAC
Cuts at: 0 1751 2859
Size: 1751 1108
Bsu36I CC'TnA_GG
Cuts at: 0 677 953 2859
Size: 677 276 1906
Fragments arranged by size:
1906 677 276
BtgI C'CryG_G
Cuts at: 0 1454 1760 2859
Size: 1454 306 1099
Fragments arranged by size:
1454 1099 306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 432 2859
Size: 432 2427
BtsI GCAGTG_nn'
Cuts at: 0 918 2859
Size: 918 1941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1341 1376 2859
Size: 1341 35 1483
Fragments arranged by size:
1483 1341 35
EaeI y'GGCC_r
Cuts at: 0 11 2174 2859
Size: 11 2163 685
Fragments arranged by size:
2163 685 11
EagI C'GGCC_G
Cuts at: 0 11 2859
Size: 11 2848
EarI CTCTTCn'nnn_
Cuts at: 0 35 265 1676 2859
Size: 35 230 1411 1183
Fragments arranged by size:
1411 1183 230 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 484 523 1280 2054 2761 2859
Size: 377 107 39 757 774 707 98
Fragments arranged by size:
774 757 707 377 107 98 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1282 1504 2374 2859
Size: 1282 222 870 485
Fragments arranged by size:
1282 870 485 222
EcoO109I rG'GnC_Cy
Cuts at: 0 255 1722 2670 2859
Size: 255 1467 948 189
Fragments arranged by size:
1467 948 255 189
EcoRI G'AATT_C
Cuts at: 0 1083 2859
Size: 1083 1776
EcoRV GAT'ATC
Cuts at: 0 693 1413 2859
Size: 693 720 1446
Fragments arranged by size:
1446 720 693
HaeII r_GCGC'y
Cuts at: 0 2189 2859
Size: 2189 670
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 467 499 720 752 868 900 2717 2749
Size: 467 32 221 32 116 32 1817 32
Cuts at: 2749 2859
Size: 110
Fragments arranged by size:
1817 467 221 116 110 32 32 32
32
MfeI C'AATT_G
Cuts at: 0 374 2859
Size: 374 2485
MscI TGG'CCA
Cuts at: 0 2176 2859
Size: 2176 683
MslI CAynn'nnrTG
Cuts at: 0 1361 2165 2859
Size: 1361 804 694
Fragments arranged by size:
1361 804 694
MspA1I CmG'CkG
Cuts at: 0 16 1534 2133 2859
Size: 16 1518 599 726
Fragments arranged by size:
1518 726 599 16
NcoI C'CATG_G
Cuts at: 0 1454 1760 2859
Size: 1454 306 1099
Fragments arranged by size:
1454 1099 306
NdeI CA'TA_TG
Cuts at: 0 2267 2859
Size: 2267 592
NheI G'CTAG_C
Cuts at: 0 766 2859
Size: 766 2093
NsiI A_TGCA'T
Cuts at: 0 313 2859
Size: 313 2546
NspI r_CATG'y
Cuts at: 0 555 1813 2859
Size: 555 1258 1046
Fragments arranged by size:
1258 1046 555
PasI CC'CwG_GG
Cuts at: 0 2199 2859
Size: 2199 660
PciI A'CATG_T
Cuts at: 0 1809 2859
Size: 1809 1050
PflMI CCAn_nnn'nTGG
Cuts at: 0 387 1193 2631 2859
Size: 387 806 1438 228
Fragments arranged by size:
1438 806 387 228
PfoI T'CCnGG_A
Cuts at: 0 861 1296 2248 2859
Size: 861 435 952 611
Fragments arranged by size:
952 861 611 435
PpuMI rG'GwC_Cy
Cuts at: 0 255 1722 2670 2859
Size: 255 1467 948 189
Fragments arranged by size:
1467 948 255 189
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 581 613 2859
Size: 581 32 2246
Fragments arranged by size:
2246 581 32
PstI C_TGCA'G
Cuts at: 0 1019 2859
Size: 1019 1840
PvuII CAG'CTG
Cuts at: 0 1534 2133 2859
Size: 1534 599 726
Fragments arranged by size:
1534 726 599
ScaI AGT'ACT
Cuts at: 0 590 2859
Size: 590 2269
SfcI C'TryA_G
Cuts at: 0 1015 1211 1754 2691 2859
Size: 1015 196 543 937 168
Fragments arranged by size:
1015 937 543 196 168
SmlI C'TyrA_G
Cuts at: 0 55 541 1393 2665 2859
Size: 55 486 852 1272 194
Fragments arranged by size:
1272 852 486 194 55
StyI C'CwwG_G
Cuts at: 0 1283 1440 1454 1545 1760 1964 2510 2859
Size: 1283 157 14 91 215 204 546 349
Fragments arranged by size:
1283 546 349 215 204 157 91 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1213 2859
Size: 1213 1646
TatI w'GTAC_w
Cuts at: 0 588 2859
Size: 588 2271
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1128 1160 1962 1994 2859
Size: 1128 32 802 32 865
Fragments arranged by size:
1128 865 802 32 32
XbaI T'CTAG_A
Cuts at: 0 423 2859
Size: 423 2436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 333 2859
Size: 333 2526
XmnI GAAnn'nnTTC
Cuts at: 0 24 2122 2859
Size: 24 2098 737
Fragments arranged by size:
2098 737 24
ZraI GAC'GTC
Cuts at: 0 483 2859
Size: 483 2376
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AhdI ApoI
AseI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsmI Bsp1286I BspEI BspHI BspMI BsrDI
BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV HaeII Hin4I MfeI MscI MslI MspA1I NcoI
NdeI NheI NsiI NspI PasI PciI PflMI PfoI
PpuMI PsrI PstI PvuII ScaI SfcI SmlI StyI
TaqII TatI TstI XbaI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AleI AlfI AloI AlwNI
ApaI ApaLI AscI AsiSI BaeI BbeI BbvCI BglII
BlpI BplI BpuEI BsaBI BsaXI BsiHKAI BsiWI BsmBI
BsrBI BsrFI BsrGI BssHII BssSI BstEII BstXI ClaI
DraI DraIII DrdI EciI EcoICRI FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MluI MmeI
NaeI NarI NgoMIV NotI NruI PacI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XhoI XmaI