Restriction map for
pFN21AB8414
(Linear) (Six-base) MAPSORT of: KIBB8414.seq Check: 6782 from: 1 to: 2739
KIBB8414.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AatII G_ACGT'C
Cuts at: 0 1156 2739
Size: 1156 1583
AccI GT'mk_AC
Cuts at: 0 101 367 2739
Size: 101 266 2372
Fragments arranged by size:
2372 266 101
Acc65I G'GTAC_C
Cuts at: 0 1037 2739
Size: 1037 1702
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 336 1457 2465 2739
Size: 223 113 1121 1008 274
Fragments arranged by size:
1121 1008 274 223 113
AfeI AGC'GCT
Cuts at: 0 2053 2739
Size: 2053 686
AflIII A'CryG_T
Cuts at: 0 1397 2236 2722 2739
Size: 1397 839 486 17
Fragments arranged by size:
1397 839 486 17
AleI CACnn'nnGTG
Cuts at: 0 416 1400 1795 2739
Size: 416 984 395 944
Fragments arranged by size:
984 944 416 395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1813 1920 2739
Size: 1813 107 819
Fragments arranged by size:
1813 819 107
AlwNI CAG_nnn'CTG
Cuts at: 0 667 2006 2389 2739
Size: 667 1339 383 350
Fragments arranged by size:
1339 667 383 350
ApaI G_GGCC'C
Cuts at: 0 201 820 2739
Size: 201 619 1919
Fragments arranged by size:
1919 619 201
ApaLI G'TGCA_C
Cuts at: 0 793 2739
Size: 793 1946
ApoI r'AATT_y
Cuts at: 0 1822 2245 2739
Size: 1822 423 494
Fragments arranged by size:
1822 494 423
AvaI C'yCGr_G
Cuts at: 0 27 64 255 2739
Size: 27 37 191 2484
Fragments arranged by size:
2484 191 37 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1021 1054 2739
Size: 1021 33 1685
Fragments arranged by size:
1685 1021 33
BamHI G'GATC_C
Cuts at: 0 305 2290 2739
Size: 305 1985 449
Fragments arranged by size:
1985 449 305
BanI G'GyrC_C
Cuts at: 0 1037 1789 2739
Size: 1037 752 950
Fragments arranged by size:
1037 950 752
BanII G_rGCy'C
Cuts at: 0 201 820 2739
Size: 201 619 1919
Fragments arranged by size:
1919 619 201
BbsI GAAGACnn'nnnn_
Cuts at: 0 1868 2739
Size: 1868 871
BbvCI CC'TCA_GC
Cuts at: 0 1161 2739
Size: 1161 1578
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 622 656 659 693 2739
Size: 622 34 3 34 2046
Fragments arranged by size:
2046 622 34 34 3
BciVI GTATCCnnnnn_n'
Cuts at: 0 2122 2184 2485 2739
Size: 2122 62 301 254
Fragments arranged by size:
2122 301 254 62
BclI T'GATC_A
Cuts at: 0 1626 2739
Size: 1626 1113
BfrBI ATG'CAT
Cuts at: 0 2111 2739
Size: 2111 628
BglI GCCn_nnn'nGGC
Cuts at: 0 1171 2027 2739
Size: 1171 856 712
Fragments arranged by size:
1171 856 712
BglII A'GATC_T
Cuts at: 0 537 1116 2739
Size: 537 579 1623
Fragments arranged by size:
1623 579 537
Bme1580I G_kGCm'C
Cuts at: 0 201 363 797 820 2070 2739
Size: 201 162 434 23 1250 669
Fragments arranged by size:
1250 669 434 201 162 23
BmgBI CAC'GTC
Cuts at: 0 576 1521 2531 2739
Size: 576 945 1010 208
Fragments arranged by size:
1010 945 576 208
BmrI ACTGGGnnnn_n'
Cuts at: 0 123 357 1157 1662 1710 2739
Size: 123 234 800 505 48 1029
Fragments arranged by size:
1029 800 505 234 123 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 1242 1534 1736 1998 2739
Size: 671 571 292 202 262 741
Fragments arranged by size:
741 671 571 292 262 202
Bpu10I CC'TnA_GC
Cuts at: 0 1161 1735 2739
Size: 1161 574 1004
Fragments arranged by size:
1161 1004 574
BsaI GGTCTCn'nnnn_
Cuts at: 0 1553 1604 2739
Size: 1553 51 1135
Fragments arranged by size:
1553 1135 51
BsaAI yAC'GTr
Cuts at: 0 792 1398 2739
Size: 792 606 1341
Fragments arranged by size:
1341 792 606
BsaBI GATnn'nnATC
Cuts at: 0 2496 2739
Size: 2496 243
BsaHI Gr'CG_yC
Cuts at: 0 1153 2739
Size: 1153 1586
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 946 976 982 1012 1777 1807 2085 2115
Size: 946 30 6 30 765 30 278 30
Cuts at: 2115 2739
Size: 624
Fragments arranged by size:
946 765 624 278 30 30 30 30
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 734 1571 2739
Size: 734 837 1168
Fragments arranged by size:
1168 837 734
BseYI C'CCAG_C
Cuts at: 0 5 394 785 812 1676 2377 2739
Size: 5 389 391 27 864 701 362
Fragments arranged by size:
864 701 391 389 362 27 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 669 1469 1819 1938 2391 2464 2739
Size: 669 800 350 119 453 73 275
Fragments arranged by size:
800 669 453 350 275 119 73
BsiHKAI G_wGCw'C
Cuts at: 0 797 1414 1520 2302 2739
Size: 797 617 106 782 437
Fragments arranged by size:
797 782 617 437 106
BsmI GAATG_Cn'
Cuts at: 0 734 2739
Size: 734 2005
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1220 2739
Size: 1220 1519
Bsp1286I G_dGCh'C
Cuts at: 0 201 363 797 820 1414 1520 2070 2302
Size: 201 162 434 23 594 106 550 232
Cuts at: 2302 2739
Size: 437
Fragments arranged by size:
594 550 437 434 232 201 162 106
23
BspHI T'CATG_A
Cuts at: 0 1661 2739
Size: 1661 1078
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 50 1888 2739
Size: 50 1838 851
Fragments arranged by size:
1838 851 50
BsrDI GCAATG_nn'
Cuts at: 0 1184 1671 1965 2541 2739
Size: 1184 487 294 576 198
Fragments arranged by size:
1184 576 487 294 198
BsrGI T'GTAC_A
Cuts at: 0 2257 2557 2725 2739
Size: 2257 300 168 14
Fragments arranged by size:
2257 300 168 14
BstAPI GCAn_nnn'nTGC
Cuts at: 0 667 2117 2405 2739
Size: 667 1450 288 334
Fragments arranged by size:
1450 667 334 288
BstEII G'GTnAC_C
Cuts at: 0 2101 2739
Size: 2101 638
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1324 1359 2739
Size: 1324 35 1380
Fragments arranged by size:
1380 1324 35
BstYI r'GATC_y
Cuts at: 0 305 537 1116 2003 2290 2739
Size: 305 232 579 887 287 449
Fragments arranged by size:
887 579 449 305 287 232
BtgI C'CryG_G
Cuts at: 0 1169 1352 1634 2097 2739
Size: 1169 183 282 463 642
Fragments arranged by size:
1169 642 463 282 183
BtsI GCAGTG_nn'
Cuts at: 0 411 423 681 957 1010 1457 1926 1953
Size: 411 12 258 276 53 447 469 27
Cuts at: 1953 2166 2226 2739
Size: 213 60 513
Fragments arranged by size:
513 469 447 411 276 258 213 60
53 27 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 742 2739
Size: 742 1997
EaeI y'GGCC_r
Cuts at: 0 1064 1565 1610 2028 2334 2739
Size: 1064 501 45 418 306 405
Fragments arranged by size:
1064 501 418 405 306 45
EarI CTCTTCn'nnn_
Cuts at: 0 463 1040 2484 2627 2739
Size: 463 577 1444 143 112
Fragments arranged by size:
1444 577 463 143 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 912 1426 2659 2739
Size: 912 514 1233 80
Fragments arranged by size:
1233 912 514 80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 336 671 1242 1457 1534 1736 1998
Size: 223 113 335 571 215 77 202 262
Cuts at: 1998 2465 2739
Size: 467 274
Fragments arranged by size:
571 467 335 274 262 223 215 202
113 77
EcoO109I rG'GnC_Cy
Cuts at: 0 197 198 266 816 1704 1900 2739
Size: 197 1 68 550 888 196 839
Fragments arranged by size:
888 839 550 197 196 68 1
EcoRI G'AATT_C
Cuts at: 0 2245 2739
Size: 2245 494
EcoRV GAT'ATC
Cuts at: 0 2517 2739
Size: 2517 222
FspI TGC'GCA
Cuts at: 0 1531 2739
Size: 1531 1208
FspAI rTGC'GCAy
Cuts at: 0 1531 2739
Size: 1531 1208
HaeII r_GCGC'y
Cuts at: 0 61 809 2055 2739
Size: 61 748 1246 684
Fragments arranged by size:
1246 748 684 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 516 548 946 978 1039 1071 1711 1743
Size: 516 32 398 32 61 32 640 32
Cuts at: 1743 2739
Size: 996
Fragments arranged by size:
996 640 516 398 61 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1506 2739
Size: 1506 1233
HindIII A'AGCT_T
Cuts at: 0 1469 2739
Size: 1469 1270
KpnI G_GTAC'C
Cuts at: 0 1041 2739
Size: 1041 1698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 333 672 2739
Size: 70 263 339 2067
Fragments arranged by size:
2067 339 263 70
MscI TGG'CCA
Cuts at: 0 1066 1567 1612 2030 2739
Size: 1066 501 45 418 709
Fragments arranged by size:
1066 709 501 418 45
MslI CAynn'nnrTG
Cuts at: 0 283 416 751 1322 1357 1400 1795 1958
Size: 283 133 335 571 35 43 395 163
Cuts at: 1958 2739
Size: 781
Fragments arranged by size:
781 571 395 335 283 163 133 43
35
MspA1I CmG'CkG
Cuts at: 0 17 667 812 1082 2018 2156 2739
Size: 17 650 145 270 936 138 583
Fragments arranged by size:
936 650 583 270 145 138 17
NcoI C'CATG_G
Cuts at: 0 1169 1352 1634 2097 2739
Size: 1169 183 282 463 642
Fragments arranged by size:
1169 642 463 282 183
NsiI A_TGCA'T
Cuts at: 0 2113 2739
Size: 2113 626
NspI r_CATG'y
Cuts at: 0 402 1240 2115 2240 2726 2739
Size: 402 838 875 125 486 13
Fragments arranged by size:
875 838 486 402 125 13
PciI A'CATG_T
Cuts at: 0 2236 2722 2739
Size: 2236 486 17
Fragments arranged by size:
2236 486 17
PflMI CCAn_nnn'nTGG
Cuts at: 0 113 401 2739
Size: 113 288 2338
Fragments arranged by size:
2338 288 113
PfoI T'CCnGG_A
Cuts at: 0 140 506 2739
Size: 140 366 2233
Fragments arranged by size:
2233 366 140
PmlI CAC'GTG
Cuts at: 0 792 1398 2739
Size: 792 606 1341
Fragments arranged by size:
1341 792 606
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1584 1616 2739
Size: 1584 32 1123
Fragments arranged by size:
1584 1123 32
PpuMI rG'GwC_Cy
Cuts at: 0 266 1704 1900 2739
Size: 266 1438 196 839
Fragments arranged by size:
1438 839 266 196
PshAI GACnn'nnGTC
Cuts at: 0 97 353 2739
Size: 97 256 2386
Fragments arranged by size:
2386 256 97
PspOMI G'GGCC_C
Cuts at: 0 197 816 2739
Size: 197 619 1923
Fragments arranged by size:
1923 619 197
PstI C_TGCA'G
Cuts at: 0 418 1017 1179 2059 2288 2739
Size: 418 599 162 880 229 451
Fragments arranged by size:
880 599 451 418 229 162
PvuII CAG'CTG
Cuts at: 0 812 2018 2156 2739
Size: 812 1206 138 583
Fragments arranged by size:
1206 812 583 138
SanDI GG'GwC_CC
Cuts at: 0 266 2739
Size: 266 2473
SbfI CC_TGCA'GG
Cuts at: 0 2288 2739
Size: 2288 451
SfcI C'TryA_G
Cuts at: 0 414 1013 1074 1112 1175 1848 2055 2284
Size: 414 599 61 38 63 673 207 229
Cuts at: 2284 2451 2739
Size: 167 288
Fragments arranged by size:
673 599 414 288 229 207 167 63
61 38
SmaI CCC'GGG
Cuts at: 0 29 2739
Size: 29 2710
SmlI C'TyrA_G
Cuts at: 0 255 2739
Size: 255 2484
StuI AGG'CCT
Cuts at: 0 905 2739
Size: 905 1834
StyI C'CwwG_G
Cuts at: 0 1033 1169 1352 1634 2097 2739
Size: 1033 136 183 282 463 642
Fragments arranged by size:
1033 642 463 282 183 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1433 1558 2384 2739
Size: 1433 125 826 355
Fragments arranged by size:
1433 826 355 125
TatI w'GTAC_w
Cuts at: 0 2239 2257 2557 2725 2739
Size: 2239 18 300 168 14
Fragments arranged by size:
2239 300 168 18 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 63 630 662 1242 1274 2085 2117
Size: 31 32 567 32 580 32 811 32
Cuts at: 2117 2205 2237 2739
Size: 88 32 502
Fragments arranged by size:
811 580 567 502 88 32 32 32
32 32 31
Tth111I GACn'n_nGTC
Cuts at: 0 1702 2739
Size: 1702 1037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 299 2739
Size: 299 2440
XhoI C'TCGA_G
Cuts at: 0 255 2739
Size: 255 2484
XmaI C'CCGG_G
Cuts at: 0 27 2739
Size: 27 2712
ZraI GAC'GTC
Cuts at: 0 1154 2739
Size: 1154 1585
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstEII
BstXI BstYI BtgI BtsI DrdI EaeI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I
HincII HindIII KpnI MmeI MscI MslI MspA1I NcoI
NsiI NspI PciI PflMI PfoI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuII SanDI SbfI SfcI SmaI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AarI AclI AflII AgeI AhdI AloI AscI AseI
AsiSI AvrII BbeI BlpI BmtI BplI BpuEI BsaWI
BsiEI BsiWI BspEI BsrBI BsrFI BssHII BssSI BstBI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI
EcoICRI EcoNI FalI FseI HpaI KasI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PasI PmeI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XmnI