Restriction map for
pFN21AB8412
(Linear) (Six-base) MAPSORT of: KIBB8412.seq Check: 5258 from: 1 to: 2769
KIBB8412.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1122 2769
Size: 1122 1647
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 385 1048 1240 1864 2047 2260 2533
Size: 130 255 663 192 624 183 213 273
Cuts at: 2533 2570 2769
Size: 37 199
Fragments arranged by size:
663 624 273 255 213 199 192 183
130 37
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1355 2769
Size: 1355 1414
AlwNI CAG_nnn'CTG
Cuts at: 0 877 1137 1230 2380 2548 2589 2661 2769
Size: 877 260 93 1150 168 41 72 108
Fragments arranged by size:
1150 877 260 168 108 93 72 41
ApaI G_GGCC'C
Cuts at: 0 85 556 1819 2769
Size: 85 471 1263 950
Fragments arranged by size:
1263 950 471 85
AvaI C'yCGr_G
Cuts at: 0 548 945 1904 2314 2769
Size: 548 397 959 410 455
Fragments arranged by size:
959 548 455 410 397
BamHI G'GATC_C
Cuts at: 0 419 839 2085 2180 2198 2769
Size: 419 420 1246 95 18 571
Fragments arranged by size:
1246 571 420 419 95 18
BanI G'GyrC_C
Cuts at: 0 140 1398 2757 2769
Size: 140 1258 1359 12
Fragments arranged by size:
1359 1258 140 12
BanII G_rGCy'C
Cuts at: 0 85 432 556 632 890 1793 1819 2675
Size: 85 347 124 76 258 903 26 856
Cuts at: 2675 2769
Size: 94
Fragments arranged by size:
903 856 347 258 124 94 85 76
26
BbsI GAAGACnn'nnnn_
Cuts at: 0 162 2769
Size: 162 2607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 912 946 2769
Size: 912 34 1823
Fragments arranged by size:
1823 912 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2294 2629 2769
Size: 2294 335 140
Fragments arranged by size:
2294 335 140
BlpI GC'TnA_GC
Cuts at: 0 431 884 998 2769
Size: 431 453 114 1771
Fragments arranged by size:
1771 453 431 114
Bme1580I G_kGCm'C
Cuts at: 0 85 320 556 1819 2769
Size: 85 235 236 1263 950
Fragments arranged by size:
1263 950 236 235 85
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 854 1436 1627 1654 2023 2527 2539
Size: 712 142 582 191 27 369 504 12
Cuts at: 2539 2638 2722 2769
Size: 99 84 47
Fragments arranged by size:
712 582 504 369 191 142 99 84
47 27 12
Bpu10I CC'TnA_GC
Cuts at: 0 523 805 2769
Size: 523 282 1964
Fragments arranged by size:
1964 523 282
BsaI GGTCTCn'nnnn_
Cuts at: 0 852 1097 2611 2769
Size: 852 245 1514 158
Fragments arranged by size:
1514 852 245 158
BsaHI Gr'CG_yC
Cuts at: 0 1012 2682 2769
Size: 1012 1670 87
Fragments arranged by size:
1670 1012 87
BsaWI w'CCGG_w
Cuts at: 0 458 2018 2267 2769
Size: 458 1560 249 502
Fragments arranged by size:
1560 502 458 249
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 580 610 946 976 1309 1339 2005 2035
Size: 580 30 336 30 333 30 666 30
Cuts at: 2035 2769
Size: 734
Fragments arranged by size:
734 666 580 336 333 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 77 95 371 1078 1324 1741 1789 1993
Size: 77 18 276 707 246 417 48 204
Cuts at: 1993 1996 2332 2536 2629 2764 2769
Size: 3 336 204 93 135 5
Fragments arranged by size:
707 417 336 276 246 204 204 135
93 77 48 18 5 3
BseYI C'CCAG_C
Cuts at: 0 263 267 372 1047 2735 2769
Size: 263 4 105 675 1688 34
Fragments arranged by size:
1688 675 263 105 34 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 2231 2769
Size: 502 1729 538
Fragments arranged by size:
1729 538 502
BsiEI CG_ry'CG
Cuts at: 0 2475 2769
Size: 2475 294
BsiHKAI G_wGCw'C
Cuts at: 0 432 2102 2327 2675 2769
Size: 432 1670 225 348 94
Fragments arranged by size:
1670 432 348 225 94
BsmI GAATG_Cn'
Cuts at: 0 138 330 2769
Size: 138 192 2439
Fragments arranged by size:
2439 192 138
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1003 2769
Size: 1003 1766
Bsp1286I G_dGCh'C
Cuts at: 0 85 320 432 556 632 890 1793 1819
Size: 85 235 112 124 76 258 903 26
Cuts at: 1819 2102 2327 2675 2769
Size: 283 225 348 94
Fragments arranged by size:
903 348 283 258 235 225 124 112
94 85 76 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 668 1122 1238 1283 1527 2351 2769
Size: 668 454 116 45 244 824 418
Fragments arranged by size:
824 668 454 418 244 116 45
BsrBI CCG'CTC
Cuts at: 0 1190 2769
Size: 1190 1579
BsrFI r'CCGG_y
Cuts at: 0 2162 2474 2754 2769
Size: 2162 312 280 15
Fragments arranged by size:
2162 312 280 15
BssHII G'CGCG_C
Cuts at: 0 2451 2769
Size: 2451 318
BstAPI GCAn_nnn'nTGC
Cuts at: 0 660 1405 2589 2769
Size: 660 745 1184 180
Fragments arranged by size:
1184 745 660 180
BstBI TT'CG_AA
Cuts at: 0 922 2769
Size: 922 1847
BstEII G'GTnAC_C
Cuts at: 0 2244 2769
Size: 2244 525
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2082 2229 2769
Size: 2082 147 540
Fragments arranged by size:
2082 540 147
BstYI r'GATC_y
Cuts at: 0 419 839 1595 2085 2180 2198 2495 2705
Size: 419 420 756 490 95 18 297 210
Cuts at: 2705 2769
Size: 64
Fragments arranged by size:
756 490 420 419 297 210 95 64
18
BtgI C'CryG_G
Cuts at: 0 1560 2769
Size: 1560 1209
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1512 2769
Size: 1512 1257
BtsI GCAGTG_nn'
Cuts at: 0 320 514 893 1076 2769
Size: 320 194 379 183 1693
Fragments arranged by size:
1693 379 320 194 183
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2130 2165 2769
Size: 2130 35 604
Fragments arranged by size:
2130 604 35
EaeI y'GGCC_r
Cuts at: 0 113 1619 1733 2472 2769
Size: 113 1506 114 739 297
Fragments arranged by size:
1506 739 297 114 113
EagI C'GGCC_G
Cuts at: 0 2472 2769
Size: 2472 297
EarI CTCTTCn'nnn_
Cuts at: 0 80 215 389 502 855 945 1055 1881
Size: 80 135 174 113 353 90 110 826
Cuts at: 1881 2061 2332 2638 2769
Size: 180 271 306 131
Fragments arranged by size:
826 353 306 271 180 174 135 131
113 110 90 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2620 2647 2769
Size: 2620 27 122
Fragments arranged by size:
2620 122 27
EcoICRI GAG'CTC
Cuts at: 0 430 2673 2769
Size: 430 2243 96
Fragments arranged by size:
2243 430 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 385 712 854 1048 1240 1436 1627
Size: 130 255 327 142 194 192 196 191
Cuts at: 1627 1654 1864 2023 2047 2260 2527 2533 2539
Size: 27 210 159 24 213 267 6 6
Cuts at: 2539 2570 2638 2722 2769
Size: 31 68 84 47
Fragments arranged by size:
327 267 255 213 210 196 194 192
191 159 142 130 84 68 47 31
27 24 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2314 2769
Size: 2314 455
EcoO109I rG'GnC_Cy
Cuts at: 0 82 552 553 1240 1320 1497 1815 1836
Size: 82 470 1 687 80 177 318 21
Cuts at: 1836 1923 2295 2769
Size: 87 372 474
Fragments arranged by size:
687 474 470 372 318 177 87 82
80 21 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1616 2769
Size: 1616 1153
FspI TGC'GCA
Cuts at: 0 1163 2769
Size: 1163 1606
HaeII r_GCGC'y
Cuts at: 0 18 735 1212 2769
Size: 18 717 477 1557
Fragments arranged by size:
1557 717 477 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 580 612 754 786 2005 2037 2769
Size: 580 32 142 32 1219 32 732
Fragments arranged by size:
1219 732 580 142 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 548 932 2769
Size: 374 174 384 1837
Fragments arranged by size:
1837 384 374 174
MscI TGG'CCA
Cuts at: 0 115 2769
Size: 115 2654
MspA1I CmG'CkG
Cuts at: 0 21 165 267 615 1157 1842 2229 2471
Size: 21 144 102 348 542 685 387 242
Cuts at: 2471 2658 2700 2712 2735 2769
Size: 187 42 12 23 34
Fragments arranged by size:
685 542 387 348 242 187 144 102
42 34 23 21 12
NaeI GCC'GGC
Cuts at: 0 2476 2756 2769
Size: 2476 280 13
Fragments arranged by size:
2476 280 13
NcoI C'CATG_G
Cuts at: 0 1560 2769
Size: 1560 1209
NgoMIV G'CCGG_C
Cuts at: 0 2474 2754 2769
Size: 2474 280 15
Fragments arranged by size:
2474 280 15
NspI r_CATG'y
Cuts at: 0 394 978 2073 2769
Size: 394 584 1095 696
Fragments arranged by size:
1095 696 584 394
PasI CC'CwG_GG
Cuts at: 0 402 632 2769
Size: 402 230 2137
Fragments arranged by size:
2137 402 230
PflMI CCAn_nnn'nTGG
Cuts at: 0 877 1205 2505 2715 2769
Size: 877 328 1300 210 54
Fragments arranged by size:
1300 877 328 210 54
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2035 2067 2769
Size: 2035 32 702
Fragments arranged by size:
2035 702 32
PpuMI rG'GwC_Cy
Cuts at: 0 1240 1923 2769
Size: 1240 683 846
Fragments arranged by size:
1240 846 683
PsiI TTA'TAA
Cuts at: 0 1670 2769
Size: 1670 1099
PspOMI G'GGCC_C
Cuts at: 0 81 552 1815 2769
Size: 81 471 1263 954
Fragments arranged by size:
1263 954 471 81
PstI C_TGCA'G
Cuts at: 0 900 1235 1344 2450 2669 2699 2769
Size: 900 335 109 1106 219 30 70
Fragments arranged by size:
1106 900 335 219 109 70 30
PvuII CAG'CTG
Cuts at: 0 267 2229 2700 2735 2769
Size: 267 1962 471 35 34
Fragments arranged by size:
1962 471 267 35 34
RsrII CG'GwC_CG
Cuts at: 0 2021 2769
Size: 2021 748
SacI G_AGCT'C
Cuts at: 0 432 2675 2769
Size: 432 2243 94
Fragments arranged by size:
2243 432 94
SanDI GG'GwC_CC
Cuts at: 0 1240 2769
Size: 1240 1529
SapI GCTCTTCn'nnn_
Cuts at: 0 2332 2638 2769
Size: 2332 306 131
Fragments arranged by size:
2332 306 131
SfcI C'TryA_G
Cuts at: 0 896 1231 1340 1659 1823 2446 2665 2695
Size: 896 335 109 319 164 623 219 30
Cuts at: 2695 2769
Size: 74
Fragments arranged by size:
896 623 335 319 219 164 109 74
30
SgrAI Cr'CCGG_yG
Cuts at: 0 2162 2769
Size: 2162 607
SmaI CCC'GGG
Cuts at: 0 550 947 1906 2769
Size: 550 397 959 863
Fragments arranged by size:
959 863 550 397
SphI G_CATG'C
Cuts at: 0 978 2769
Size: 978 1791
StyI C'CwwG_G
Cuts at: 0 649 1003 1409 1560 1736 1751 1793 2075
Size: 649 354 406 151 176 15 42 282
Cuts at: 2075 2193 2402 2769
Size: 118 209 367
Fragments arranged by size:
649 406 367 354 282 209 176 151
118 42 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 259 2727 2769
Size: 259 2468 42
Fragments arranged by size:
2468 259 42
TatI w'GTAC_w
Cuts at: 0 2279 2769
Size: 2279 490
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1309 1341 2769
Size: 1309 32 1428
Fragments arranged by size:
1428 1309 32
XbaI T'CTAG_A
Cuts at: 0 1432 2769
Size: 1432 1337
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1651 2565 2769
Size: 1651 914 204
Fragments arranged by size:
1651 914 204
XmaI C'CCGG_G
Cuts at: 0 548 945 1904 2769
Size: 548 397 959 865
Fragments arranged by size:
959 865 548 397
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI AvaI BamHI BanI
BanII BbsI BcgI BglI BlpI Bme1580I BpmI Bpu10I
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I MmeI MscI MspA1I
NaeI NcoI NgoMIV NspI PasI PflMI PpiI PpuMI
PsiI PspOMI PstI PvuII RsrII SacI SanDI SapI
SfcI SgrAI SmaI SphI StyI TaqII TatI TstI
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BciVI BclI BfrBI BglII
BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsiWI
BspEI BspHI BsrDI BsrGI BssSI BstZ17I Bsu36I ClaI
DraI DraIII DrdI EcoRI EcoRV FseI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MslI NarI
NdeI NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PshAI PspXI PsrI PvuI SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SmlI SnaBI
SpeI SrfI SspI StuI SwaI Tth111I XhoI XmnI
ZraI