Restriction map for
pFN21AB8349
(Linear) (Six-base) MAPSORT of: KIBB8349.seq Check: 7508 from: 1 to: 786
KIBB8349.orf
With 182 enzymes: SgfI *
July 3, 2012 16:33 ..
AatII G_ACGT'C
Cuts at: 0 569 786
Size: 569 217
AccI GT'mk_AC
Cuts at: 0 305 786
Size: 305 481
Acc65I G'GTAC_C
Cuts at: 0 143 786
Size: 143 643
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 246 621 760 786
Size: 214 32 375 139 26
Fragments arranged by size:
375 214 139 32 26
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 246 295 786
Size: 246 49 491
Fragments arranged by size:
491 246 49
AlwNI CAG_nnn'CTG
Cuts at: 0 738 786
Size: 738 48
ApaI G_GGCC'C
Cuts at: 0 120 786
Size: 120 666
ApoI r'AATT_y
Cuts at: 0 727 786
Size: 727 59
AvaI C'yCGr_G
Cuts at: 0 139 655 786
Size: 139 516 131
Fragments arranged by size:
516 139 131
BamHI G'GATC_C
Cuts at: 0 164 786
Size: 164 622
BanI G'GyrC_C
Cuts at: 0 33 85 143 383 786
Size: 33 52 58 240 403
Fragments arranged by size:
403 240 58 52 33
BanII G_rGCy'C
Cuts at: 0 120 786
Size: 120 666
BbeI G_GCGC'C
Cuts at: 0 387 786
Size: 387 399
BbsI GAAGACnn'nnnn_
Cuts at: 0 748 786
Size: 748 38
BglI GCCn_nnn'nGGC
Cuts at: 0 429 786
Size: 429 357
Bme1580I G_kGCm'C
Cuts at: 0 88 120 645 786
Size: 88 32 525 141
Fragments arranged by size:
525 141 88 32
BmgBI CAC'GTC
Cuts at: 0 528 786
Size: 528 258
BmrI ACTGGGnnnn_n'
Cuts at: 0 363 786
Size: 363 423
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 267 786
Size: 267 519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 786
Size: 757 29
BsaAI yAC'GTr
Cuts at: 0 12 786
Size: 12 774
BsaHI Gr'CG_yC
Cuts at: 0 302 384 566 786
Size: 302 82 182 220
Fragments arranged by size:
302 220 182 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 287 317 664 694 786
Size: 287 30 347 30 92
Fragments arranged by size:
347 287 92 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 308 786
Size: 308 478
BsiEI CG_ry'CG
Cuts at: 0 158 786
Size: 158 628
Bsp1286I G_dGCh'C
Cuts at: 0 88 120 645 786
Size: 88 32 525 141
Fragments arranged by size:
525 141 88 32
BsrBI CCG'CTC
Cuts at: 0 42 660 786
Size: 42 618 126
Fragments arranged by size:
618 126 42
BstYI r'GATC_y
Cuts at: 0 164 786
Size: 164 622
BtgI C'CryG_G
Cuts at: 0 29 345 786
Size: 29 316 441
Fragments arranged by size:
441 316 29
EaeI y'GGCC_r
Cuts at: 0 19 56 155 786
Size: 19 37 99 631
Fragments arranged by size:
631 99 37 19
EagI C'GGCC_G
Cuts at: 0 155 786
Size: 155 631
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 166 572 685 786
Size: 166 406 113 101
Fragments arranged by size:
406 166 113 101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 246 621 757 760 786
Size: 214 32 375 136 3 26
Fragments arranged by size:
375 214 136 32 26 3
EcoO109I rG'GnC_Cy
Cuts at: 0 483 786
Size: 483 303
EcoRI G'AATT_C
Cuts at: 0 727 786
Size: 727 59
HaeII r_GCGC'y
Cuts at: 0 387 786
Size: 387 399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 287 319 454 486 596 628 664 696
Size: 287 32 135 32 110 32 36 32
Cuts at: 696 786
Size: 90
Fragments arranged by size:
287 135 110 90 36 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 383 786
Size: 383 403
KpnI G_GTAC'C
Cuts at: 0 147 786
Size: 147 639
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 786
Size: 187 599
MscI TGG'CCA
Cuts at: 0 58 786
Size: 58 728
MslI CAynn'nnrTG
Cuts at: 0 437 786
Size: 437 349
MspA1I CmG'CkG
Cuts at: 0 31 95 220 786
Size: 31 64 125 566
Fragments arranged by size:
566 125 64 31
NarI GG'CG_CC
Cuts at: 0 384 786
Size: 384 402
NcoI C'CATG_G
Cuts at: 0 345 786
Size: 345 441
NspI r_CATG'y
Cuts at: 0 436 786
Size: 436 350
PflMI CCAn_nnn'nTGG
Cuts at: 0 113 786
Size: 113 673
PmlI CAC'GTG
Cuts at: 0 12 786
Size: 12 774
PspOMI G'GGCC_C
Cuts at: 0 116 786
Size: 116 670
PspXI vC'TCGA_Gb
Cuts at: 0 655 786
Size: 655 131
PstI C_TGCA'G
Cuts at: 0 219 225 555 786
Size: 219 6 330 231
Fragments arranged by size:
330 231 219 6
PvuII CAG'CTG
Cuts at: 0 95 220 786
Size: 95 125 566
Fragments arranged by size:
566 125 95
SacII CC_GC'GG
Cuts at: 0 32 786
Size: 32 754
SfcI C'TryA_G
Cuts at: 0 90 215 221 551 786
Size: 90 125 6 330 235
Fragments arranged by size:
330 235 125 90 6
SfoI GGC'GCC
Cuts at: 0 385 786
Size: 385 401
SmaI CCC'GGG
Cuts at: 0 141 786
Size: 141 645
SmlI C'TyrA_G
Cuts at: 0 655 786
Size: 655 131
StyI C'CwwG_G
Cuts at: 0 239 345 786
Size: 239 106 441
Fragments arranged by size:
441 239 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 345 786
Size: 345 441
TatI w'GTAC_w
Cuts at: 0 651 786
Size: 651 135
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 527 775 786
Size: 527 248 11
Fragments arranged by size:
527 248 11
XhoI C'TCGA_G
Cuts at: 0 655 786
Size: 655 131
XmaI C'CCGG_G
Cuts at: 0 139 786
Size: 139 647
XmnI GAAnn'nnTTC
Cuts at: 0 634 786
Size: 634 152
ZraI GAC'GTC
Cuts at: 0 567 786
Size: 567 219
Enzymes that do cut:
AatII AccI Acc65I AcuI AlfI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbeI BbsI BglI Bme1580I
BmgBI BmrI BplI BpmI BsaAI BsaHI BsaXI BseRI
BsiEI Bsp1286I BsrBI BstYI BtgI EaeI EagI EciI
Eco57MI EcoO109I EcoRI HaeII Hin4I KasI KpnI MmeI
MscI MslI MspA1I NarI NcoI NspI PflMI PmlI
PspOMI PspXI PstI PvuII SacII SfcI SfoI SmaI
SmlI StyI TaqII TatI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmtI Bpu10I
BpuEI BsaI BsaBI BsaWI BseYI BsgI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI