Restriction map for
pFN21AB8316
(Linear) (Six-base) MAPSORT of: KIBB8316.seq Check: 5935 from: 1 to: 1269
KIBB8316.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AatII G_ACGT'C
Cuts at: 0 257 419 1269
Size: 257 162 850
Fragments arranged by size:
850 257 162
Acc65I G'GTAC_C
Cuts at: 0 1098 1269
Size: 1098 171
AfeI AGC'GCT
Cuts at: 0 950 1269
Size: 950 319
AflIII A'CryG_T
Cuts at: 0 728 1269
Size: 728 541
AgeI A'CCGG_T
Cuts at: 0 1101 1269
Size: 1101 168
AhdI GACnn_n'nnGTC
Cuts at: 0 153 1269
Size: 153 1116
AleI CACnn'nnGTG
Cuts at: 0 922 1269
Size: 922 347
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 211 243 1269
Size: 211 32 1026
Fragments arranged by size:
1026 211 32
AlwNI CAG_nnn'CTG
Cuts at: 0 306 682 712 1199 1269
Size: 306 376 30 487 70
Fragments arranged by size:
487 376 306 70 30
ApaI G_GGCC'C
Cuts at: 0 1235 1269
Size: 1235 34
ApoI r'AATT_y
Cuts at: 0 45 990 1269
Size: 45 945 279
Fragments arranged by size:
945 279 45
AvaI C'yCGr_G
Cuts at: 0 150 1269
Size: 150 1119
BamHI G'GATC_C
Cuts at: 0 688 1269
Size: 688 581
BanI G'GyrC_C
Cuts at: 0 78 699 834 1098 1269
Size: 78 621 135 264 171
Fragments arranged by size:
621 264 171 135 78
BanII G_rGCy'C
Cuts at: 0 169 1235 1269
Size: 169 1066 34
Fragments arranged by size:
1066 169 34
BclI T'GATC_A
Cuts at: 0 374 1269
Size: 374 895
Bme1580I G_kGCm'C
Cuts at: 0 1235 1269
Size: 1235 34
BmgBI CAC'GTC
Cuts at: 0 731 915 1205 1269
Size: 731 184 290 64
Fragments arranged by size:
731 290 184 64
BmrI ACTGGGnnnn_n'
Cuts at: 0 87 170 1269
Size: 87 83 1099
Fragments arranged by size:
1099 87 83
BmtI G_CTAG'C
Cuts at: 0 1226 1269
Size: 1226 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 44 1269
Size: 44 1225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 1269
Size: 195 1074
BsaI GGTCTCn'nnnn_
Cuts at: 0 100 552 1269
Size: 100 452 717
Fragments arranged by size:
717 452 100
BsaHI Gr'CG_yC
Cuts at: 0 254 416 495 1269
Size: 254 162 79 774
Fragments arranged by size:
774 254 162 79
BsaWI w'CCGG_w
Cuts at: 0 921 1101 1269
Size: 921 180 168
Fragments arranged by size:
921 180 168
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 601 679 1269
Size: 271 330 78 590
Fragments arranged by size:
590 330 271 78
BseYI C'CCAG_C
Cuts at: 0 120 875 1120 1167 1269
Size: 120 755 245 47 102
Fragments arranged by size:
755 245 120 102 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 1049 1112 1159 1185 1269
Size: 632 417 63 47 26 84
Fragments arranged by size:
632 417 84 63 47 26
BsiEI CG_ry'CG
Cuts at: 0 779 884 1269
Size: 779 105 385
Fragments arranged by size:
779 385 105
BsiHKAI G_wGCw'C
Cuts at: 0 1267 1269
Size: 1267 2
BsmBI CGTCTCn'nnnn_
Cuts at: 0 737 1269
Size: 737 532
Bsp1286I G_dGCh'C
Cuts at: 0 169 1235 1267 1269
Size: 169 1066 32 2
Fragments arranged by size:
1066 169 32 2
BsrDI GCAATG_nn'
Cuts at: 0 1182 1269
Size: 1182 87
BsrFI r'CCGG_y
Cuts at: 0 774 778 879 883 1101 1269
Size: 774 4 101 4 218 168
Fragments arranged by size:
774 218 168 101 4 4
BssSI C'ACGA_G
Cuts at: 0 613 1269
Size: 613 656
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1135 1269
Size: 1135 134
BstEII G'GTnAC_C
Cuts at: 0 1104 1269
Size: 1104 165
BstYI r'GATC_y
Cuts at: 0 126 688 973 1269
Size: 126 562 285 296
Fragments arranged by size:
562 296 285 126
BtgI C'CryG_G
Cuts at: 0 848 1043 1145 1269
Size: 848 195 102 124
Fragments arranged by size:
848 195 124 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1241 1269
Size: 1241 28
BtsI GCAGTG_nn'
Cuts at: 0 1197 1269
Size: 1197 72
DraIII CAC_nnn'GTG
Cuts at: 0 429 1269
Size: 429 840
DrdI GACnn_nn'nnGTC
Cuts at: 0 253 1269
Size: 253 1016
EaeI y'GGCC_r
Cuts at: 0 212 776 881 926 1269
Size: 212 564 105 45 343
Fragments arranged by size:
564 343 212 105 45
EagI C'GGCC_G
Cuts at: 0 776 881 1269
Size: 776 105 388
Fragments arranged by size:
776 388 105
EarI CTCTTCn'nnn_
Cuts at: 0 619 624 1269
Size: 619 5 645
Fragments arranged by size:
645 619 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 451 834 1269
Size: 451 383 435
Fragments arranged by size:
451 435 383
EcoO109I rG'GnC_Cy
Cuts at: 0 512 1232 1269
Size: 512 720 37
Fragments arranged by size:
720 512 37
EcoRI G'AATT_C
Cuts at: 0 990 1269
Size: 990 279
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 98 474 1269
Size: 98 376 795
Fragments arranged by size:
795 376 98
HaeII r_GCGC'y
Cuts at: 0 952 1269
Size: 952 317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 139 171 623 655 715 747 1269
Size: 30 109 32 452 32 60 32 522
Fragments arranged by size:
522 452 109 60 32 32 32 30
HindIII A'AGCT_T
Cuts at: 0 221 1269
Size: 221 1048
KpnI G_GTAC'C
Cuts at: 0 1102 1269
Size: 1102 167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 1269
Size: 228 1041
MslI CAynn'nnrTG
Cuts at: 0 922 985 1150 1269
Size: 922 63 165 119
Fragments arranged by size:
922 165 119 63
MspA1I CmG'CkG
Cuts at: 0 931 1269
Size: 931 338
NaeI GCC'GGC
Cuts at: 0 881 885 1269
Size: 881 4 384
Fragments arranged by size:
881 384 4
NcoI C'CATG_G
Cuts at: 0 1043 1145 1269
Size: 1043 102 124
Fragments arranged by size:
1043 124 102
NgoMIV G'CCGG_C
Cuts at: 0 879 883 1269
Size: 879 4 386
Fragments arranged by size:
879 386 4
NheI G'CTAG_C
Cuts at: 0 1222 1269
Size: 1222 47
NspI r_CATG'y
Cuts at: 0 1077 1218 1269
Size: 1077 141 51
Fragments arranged by size:
1077 141 51
PasI CC'CwG_GG
Cuts at: 0 264 937 1269
Size: 264 673 332
Fragments arranged by size:
673 332 264
PflMI CCAn_nnn'nTGG
Cuts at: 0 210 347 1269
Size: 210 137 922
Fragments arranged by size:
922 210 137
PpuMI rG'GwC_Cy
Cuts at: 0 512 1269
Size: 512 757
PshAI GACnn'nnGTC
Cuts at: 0 863 1269
Size: 863 406
PspOMI G'GGCC_C
Cuts at: 0 1231 1269
Size: 1231 38
PstI C_TGCA'G
Cuts at: 0 948 1269
Size: 948 321
SexAI A'CCwGG_T
Cuts at: 0 749 1269
Size: 749 520
SfcI C'TryA_G
Cuts at: 0 944 1269
Size: 944 325
SmaI CCC'GGG
Cuts at: 0 152 1269
Size: 152 1117
SmlI C'TyrA_G
Cuts at: 0 174 1269
Size: 174 1095
SphI G_CATG'C
Cuts at: 0 1077 1269
Size: 1077 192
StyI C'CwwG_G
Cuts at: 0 1043 1145 1269
Size: 1043 102 124
Fragments arranged by size:
1043 124 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 134 625 805 1269
Size: 134 491 180 464
Fragments arranged by size:
491 464 180 134
TatI w'GTAC_w
Cuts at: 0 1211 1269
Size: 1211 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1044 1076 1269
Size: 1044 32 193
Fragments arranged by size:
1044 193 32
Tth111I GACn'n_nGTC
Cuts at: 0 751 1269
Size: 751 518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 305 799 1269
Size: 305 494 470
Fragments arranged by size:
494 470 305
XmaI C'CCGG_G
Cuts at: 0 150 1269
Size: 150 1119
ZraI GAC'GTC
Cuts at: 0 255 417 1269
Size: 255 162 852
Fragments arranged by size:
852 255 162
Enzymes that do cut:
AatII Acc65I AfeI AflIII AgeI AhdI AleI AloI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BclI
Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BsrDI BsrFI BssSI BstAPI BstEII BstYI BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoO109I
EcoRI FalI HaeII Hin4I HindIII KpnI MmeI MslI
MspA1I NaeI NcoI NgoMIV NheI NspI PasI PflMI
PpuMI PshAI PspOMI PstI SexAI SfcI SmaI SmlI
SphI StyI TaqII TatI TstI Tth111I XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AflII AlfI ApaLI AscI
AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI BpmI Bpu10I BsaAI
BsaBI BsaXI BsiWI BsmI BspEI BspHI BspMI BsrBI
BsrGI BssHII BstBI BstXI BstZ17I Bsu36I ClaI CspCI
DraI EcoICRI Eco57MI EcoNI EcoRV FseI FspI FspAI
HincII HpaI KasI MfeI MluI MscI NarI NdeI
NotI NruI NsiI PacI PciI PfoI PmeI PmlI
PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
XbaI XhoI XmnI