(Linear) (Six-base) MAPSORT of: KIBB8312.seq  Check: 3453  from: 1  to: 426



KIBB8312.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

ApoI r'AATT_y

Cuts at:      0     298     367     426 
   Size:        298      69      59

  Fragments arranged by size: 

                 298      69      59
 
BanI G'GyrC_C

Cuts at:      0       4     426 
   Size:          4     422
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     372     406     426 
   Size:        372      34      20

  Fragments arranged by size: 

                 372      34      20
 
Bme1580I G_kGCm'C

Cuts at:      0       9     426 
   Size:          9     417
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     159     426 
   Size:        159     267
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     426 
   Size:         25     401
 
BsaWI w'CCGG_w

Cuts at:      0     244     426 
   Size:        244     182
 
BseYI C'CCAG_C

Cuts at:      0     197     426 
   Size:        197     229
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      81     426 
   Size:         81     345
 
BsmI GAATG_Cn'

Cuts at:      0     237     426 
   Size:        237     189
 
Bsp1286I G_dGCh'C

Cuts at:      0       9     426 
   Size:          9     417
 
BsrBI CCG'CTC

Cuts at:      0     108     426 
   Size:        108     318
 
BtgI C'CryG_G

Cuts at:      0     419     426 
   Size:        419       7
 
BtsI GCAGTG_nn'

Cuts at:      0     132     426 
   Size:        132     294
 
EaeI y'GGCC_r

Cuts at:      0     113     426 
   Size:        113     313
 
MspA1I CmG'CkG

Cuts at:      0     411     426 
   Size:        411      15
 
NcoI C'CATG_G

Cuts at:      0     419     426 
   Size:        419       7
 
PvuII CAG'CTG

Cuts at:      0     411     426 
   Size:        411      15
 
SmlI C'TyrA_G

Cuts at:      0      40     426 
   Size:         40     386
 
StyI C'CwwG_G

Cuts at:      0     291     419     426 
   Size:        291     128       7

  Fragments arranged by size: 

                 291     128       7
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     408     426 
   Size:        408      18
 

 Enzymes that do cut:

     ApoI     BanI     BcgI Bme1580I     BmrI    BpuEI    BsaWI    BseYI
     BsgI     BsmI Bsp1286I    BsrBI     BtgI     BtsI     EaeI   MspA1I
     NcoI    PvuII     SmlI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI