Restriction map for
pFN21AB8296
(Linear) (Six-base) MAPSORT of: KIBB8296.seq Check: 4037 from: 1 to: 1266
KIBB8296.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 973 1266
Size: 383 590 293
Fragments arranged by size:
590 383 293
AleI CACnn'nnGTG
Cuts at: 0 1253 1266
Size: 1253 13
ApaI G_GGCC'C
Cuts at: 0 763 1266
Size: 763 503
ApoI r'AATT_y
Cuts at: 0 962 1266
Size: 962 304
AvaI C'yCGr_G
Cuts at: 0 36 1214 1266
Size: 36 1178 52
Fragments arranged by size:
1178 52 36
BanI G'GyrC_C
Cuts at: 0 183 423 1223 1266
Size: 183 240 800 43
Fragments arranged by size:
800 240 183 43
BanII G_rGCy'C
Cuts at: 0 391 763 854 1121 1266
Size: 391 372 91 267 145
Fragments arranged by size:
391 372 267 145 91
BbeI G_GCGC'C
Cuts at: 0 427 1227 1266
Size: 427 800 39
Fragments arranged by size:
800 427 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 197 581 1266
Size: 197 384 685
Fragments arranged by size:
685 384 197
BbvCI CC'TCA_GC
Cuts at: 0 685 1266
Size: 685 581
BclI T'GATC_A
Cuts at: 0 1011 1266
Size: 1011 255
BlpI GC'TnA_GC
Cuts at: 0 53 1266
Size: 53 1213
Bme1580I G_kGCm'C
Cuts at: 0 763 977 1266
Size: 763 214 289
Fragments arranged by size:
763 289 214
BmgBI CAC'GTC
Cuts at: 0 594 1266
Size: 594 672
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1105 1226 1266
Size: 1105 121 40
Fragments arranged by size:
1105 121 40
Bpu10I CC'TnA_GC
Cuts at: 0 542 685 729 1266
Size: 542 143 44 537
Fragments arranged by size:
542 537 143 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 886 1266
Size: 320 566 380
Fragments arranged by size:
566 380 320
BsaI GGTCTCn'nnnn_
Cuts at: 0 319 1266
Size: 319 947
BsaHI Gr'CG_yC
Cuts at: 0 424 1224 1262 1266
Size: 424 800 38 4
Fragments arranged by size:
800 424 38 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 364 787 1266
Size: 364 423 479
Fragments arranged by size:
479 423 364
BseYI C'CCAG_C
Cuts at: 0 555 600 1266
Size: 555 45 666
Fragments arranged by size:
666 555 45
BsiHKAI G_wGCw'C
Cuts at: 0 461 1142 1266
Size: 461 681 124
Fragments arranged by size:
681 461 124
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1091 1266
Size: 1091 175
Bsp1286I G_dGCh'C
Cuts at: 0 391 461 763 854 977 1121 1142 1266
Size: 391 70 302 91 123 144 21 124
Fragments arranged by size:
391 302 144 124 123 91 70 21
BsrDI GCAATG_nn'
Cuts at: 0 1175 1266
Size: 1175 91
BsrFI r'CCGG_y
Cuts at: 0 357 1266
Size: 357 909
BssHII G'CGCG_C
Cuts at: 0 30 1266
Size: 30 1236
BssSI C'ACGA_G
Cuts at: 0 691 1266
Size: 691 575
BstAPI GCAn_nnn'nTGC
Cuts at: 0 981 1266
Size: 981 285
BstYI r'GATC_y
Cuts at: 0 1109 1266
Size: 1109 157
Bsu36I CC'TnA_GG
Cuts at: 0 764 1266
Size: 764 502
BtgI C'CryG_G
Cuts at: 0 47 1266
Size: 47 1219
BtsI GCAGTG_nn'
Cuts at: 0 1248 1260 1266
Size: 1248 12 6
Fragments arranged by size:
1248 12 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 429 464 1266
Size: 429 35 802
Fragments arranged by size:
802 429 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 835 1266
Size: 835 431
EaeI y'GGCC_r
Cuts at: 0 635 782 1266
Size: 635 147 484
Fragments arranged by size:
635 484 147
EarI CTCTTCn'nnn_
Cuts at: 0 908 1266
Size: 908 358
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1150 1266
Size: 1150 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 973 1105 1226 1266
Size: 383 590 132 121 40
Fragments arranged by size:
590 383 132 121 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 904 1266
Size: 904 362
EcoO109I rG'GnC_Cy
Cuts at: 0 15 61 249 760 1266
Size: 15 46 188 511 506
Fragments arranged by size:
511 506 188 46 15
EcoRI G'AATT_C
Cuts at: 0 962 1266
Size: 962 304
EcoRV GAT'ATC
Cuts at: 0 609 1266
Size: 609 657
HaeII r_GCGC'y
Cuts at: 0 217 427 1227 1266
Size: 217 210 800 39
Fragments arranged by size:
800 217 210 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 63 95 318 350 503 535 1266
Size: 63 32 223 32 153 32 731
Fragments arranged by size:
731 223 153 63 32 32 32
KasI G'GCGC_C
Cuts at: 0 423 1223 1266
Size: 423 800 43
Fragments arranged by size:
800 423 43
MslI CAynn'nnrTG
Cuts at: 0 1253 1266
Size: 1253 13
MspA1I CmG'CkG
Cuts at: 0 604 874 1266
Size: 604 270 392
Fragments arranged by size:
604 392 270
NaeI GCC'GGC
Cuts at: 0 359 1266
Size: 359 907
NarI GG'CG_CC
Cuts at: 0 424 1224 1266
Size: 424 800 42
Fragments arranged by size:
800 424 42
NgoMIV G'CCGG_C
Cuts at: 0 357 1266
Size: 357 909
PpuMI rG'GwC_Cy
Cuts at: 0 15 61 1266
Size: 15 46 1205
Fragments arranged by size:
1205 46 15
PshAI GACnn'nnGTC
Cuts at: 0 874 1266
Size: 874 392
PspOMI G'GGCC_C
Cuts at: 0 759 1266
Size: 759 507
PstI C_TGCA'G
Cuts at: 0 1255 1266
Size: 1255 11
SapI GCTCTTCn'nnn_
Cuts at: 0 908 1266
Size: 908 358
SfcI C'TryA_G
Cuts at: 0 864 1251 1266
Size: 864 387 15
Fragments arranged by size:
864 387 15
SfoI GGC'GCC
Cuts at: 0 425 1225 1266
Size: 425 800 41
Fragments arranged by size:
800 425 41
SmlI C'TyrA_G
Cuts at: 0 335 901 1266
Size: 335 566 365
Fragments arranged by size:
566 365 335
StuI AGG'CCT
Cuts at: 0 706 1266
Size: 706 560
StyI C'CwwG_G
Cuts at: 0 440 476 734 1113 1266
Size: 440 36 258 379 153
Fragments arranged by size:
440 379 258 153 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 579 1266
Size: 579 687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 477 509 1266
Size: 477 32 757
Fragments arranged by size:
757 477 32
XmnI GAAnn'nnTTC
Cuts at: 0 926 1266
Size: 926 340
Enzymes that do cut:
AcuI AleI ApaI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BclI BlpI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaHI BseRI BseYI BsiHKAI BsmBI Bsp1286I
BsrDI BsrFI BssHII BssSI BstAPI BstYI Bsu36I BtgI
BtsI CspCI DrdI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I KasI MslI MspA1I
NaeI NarI NgoMIV PpuMI PshAI PspOMI PstI SapI
SfcI SfoI SmlI StuI StyI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BfrBI BglI
BglII BmrI BmtI BplI BsaAI BsaBI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI
BsrGI BstBI BstEII BstXI BstZ17I BtgZI ClaI DraI
DraIII EagI EcoICRI FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XcmI XhoI XmaI ZraI