Restriction map for
pFN21AB8241
(Linear) (Six-base) MAPSORT of: KIBB8241.seq Check: 4988 from: 1 to: 1392
KIBB8241.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 379 498 1392
Size: 379 119 894
Fragments arranged by size:
894 379 119
AccI GT'mk_AC
Cuts at: 0 967 1392
Size: 967 425
Acc65I G'GTAC_C
Cuts at: 0 775 1392
Size: 775 617
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 229 694 1392
Size: 124 105 465 698
Fragments arranged by size:
698 465 124 105
AfeI AGC'GCT
Cuts at: 0 632 1392
Size: 632 760
AflIII A'CryG_T
Cuts at: 0 581 1392
Size: 581 811
AleI CACnn'nnGTG
Cuts at: 0 721 1392
Size: 721 671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1373 1392
Size: 1373 19
AlwNI CAG_nnn'CTG
Cuts at: 0 1311 1392
Size: 1311 81
ApaI G_GGCC'C
Cuts at: 0 218 1392
Size: 218 1174
ApoI r'AATT_y
Cuts at: 0 481 1392
Size: 481 911
AvaI C'yCGr_G
Cuts at: 0 1336 1392
Size: 1336 56
BamHI G'GATC_C
Cuts at: 0 224 1392
Size: 224 1168
BanI G'GyrC_C
Cuts at: 0 367 448 775 814 1392
Size: 367 81 327 39 578
Fragments arranged by size:
578 367 327 81 39
BanII G_rGCy'C
Cuts at: 0 218 1337 1392
Size: 218 1119 55
Fragments arranged by size:
1119 218 55
BbeI G_GCGC'C
Cuts at: 0 452 1392
Size: 452 940
BbsI GAAGACnn'nnnn_
Cuts at: 0 119 1392
Size: 119 1273
BglI GCCn_nnn'nGGC
Cuts at: 0 266 532 1216 1381 1392
Size: 266 266 684 165 11
Fragments arranged by size:
684 266 266 165 11
BglII A'GATC_T
Cuts at: 0 386 1392
Size: 386 1006
Bme1580I G_kGCm'C
Cuts at: 0 218 370 884 1051 1392
Size: 218 152 514 167 341
Fragments arranged by size:
514 341 218 167 152
BmgBI CAC'GTC
Cuts at: 0 582 696 1392
Size: 582 114 696
Fragments arranged by size:
696 582 114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1245 1392
Size: 1245 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 1392
Size: 171 1221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 1392
Size: 274 1118
BsaHI Gr'CG_yC
Cuts at: 0 449 1392
Size: 449 943
BsaWI w'CCGG_w
Cuts at: 0 116 1392
Size: 116 1276
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 859 889 1392
Size: 859 30 503
Fragments arranged by size:
859 503 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 637 880 883 1354 1392
Size: 637 243 3 471 38
Fragments arranged by size:
637 471 243 38 3
BseYI C'CCAG_C
Cuts at: 0 601 910 929 1361 1392
Size: 601 309 19 432 31
Fragments arranged by size:
601 432 309 31 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1048 1324 1392
Size: 1048 276 68
Fragments arranged by size:
1048 276 68
BsiEI CG_ry'CG
Cuts at: 0 141 1216 1220 1330 1392
Size: 141 1075 4 110 62
Fragments arranged by size:
1075 141 110 62 4
BsiHKAI G_wGCw'C
Cuts at: 0 1278 1337 1392
Size: 1278 59 55
Fragments arranged by size:
1278 59 55
BsmI GAATG_Cn'
Cuts at: 0 558 1392
Size: 558 834
BsmBI CGTCTCn'nnnn_
Cuts at: 0 991 1392
Size: 991 401
Bsp1286I G_dGCh'C
Cuts at: 0 218 370 884 1051 1278 1337 1392
Size: 218 152 514 167 227 59 55
Fragments arranged by size:
514 227 218 167 152 59 55
BspHI T'CATG_A
Cuts at: 0 302 1392
Size: 302 1090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 379 498 526 1392
Size: 379 119 28 866
Fragments arranged by size:
866 379 119 28
BsrBI CCG'CTC
Cuts at: 0 1076 1344 1392
Size: 1076 268 48
Fragments arranged by size:
1076 268 48
BsrFI r'CCGG_y
Cuts at: 0 1215 1392
Size: 1215 177
BssSI C'ACGA_G
Cuts at: 0 1126 1392
Size: 1126 266
BstXI CCAn_nnnn'nTGG
Cuts at: 0 336 723 1392
Size: 336 387 669
Fragments arranged by size:
669 387 336
BstYI r'GATC_y
Cuts at: 0 200 224 386 1392
Size: 200 24 162 1006
Fragments arranged by size:
1006 200 162 24
BtgI C'CryG_G
Cuts at: 0 261 443 768 1392
Size: 261 182 325 624
Fragments arranged by size:
624 325 261 182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1083 1392
Size: 1083 309
BtsI GCAGTG_nn'
Cuts at: 0 752 1392
Size: 752 640
EaeI y'GGCC_r
Cuts at: 0 138 512 725 837 1137 1213 1217 1327
Size: 138 374 213 112 300 76 4 110
Cuts at: 1327 1392
Size: 65
Fragments arranged by size:
374 300 213 138 112 110 76 65
4
EagI C'GGCC_G
Cuts at: 0 138 1213 1217 1327 1392
Size: 138 1075 4 110 65
Fragments arranged by size:
1075 138 110 65 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1306 1392
Size: 1306 86
EcoICRI GAG'CTC
Cuts at: 0 1335 1392
Size: 1335 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 171 229 694 1392
Size: 124 47 58 465 698
Fragments arranged by size:
698 465 124 58 47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 208 1392
Size: 208 1184
EcoO109I rG'GnC_Cy
Cuts at: 0 214 605 1392
Size: 214 391 787
Fragments arranged by size:
787 391 214
EcoRI G'AATT_C
Cuts at: 0 481 1392
Size: 481 911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 315 699 1392
Size: 315 384 693
Fragments arranged by size:
693 384 315
FseI GG_CCGG'CC
Cuts at: 0 1219 1392
Size: 1219 173
HaeII r_GCGC'y
Cuts at: 0 272 452 634 1392
Size: 272 180 182 758
Fragments arranged by size:
758 272 182 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 859 891 1260 1292 1392
Size: 859 32 369 32 100
Fragments arranged by size:
859 369 100 32 32
HincII GTy'rAC
Cuts at: 0 846 1392
Size: 846 546
KasI G'GCGC_C
Cuts at: 0 448 1392
Size: 448 944
KpnI G_GTAC'C
Cuts at: 0 779 1392
Size: 779 613
MscI TGG'CCA
Cuts at: 0 514 727 839 1139 1392
Size: 514 213 112 300 253
Fragments arranged by size:
514 300 253 213 112
MslI CAynn'nnrTG
Cuts at: 0 721 1392
Size: 721 671
MspA1I CmG'CkG
Cuts at: 0 62 1054 1353 1392
Size: 62 992 299 39
Fragments arranged by size:
992 299 62 39
NaeI GCC'GGC
Cuts at: 0 1217 1392
Size: 1217 175
NarI GG'CG_CC
Cuts at: 0 449 1392
Size: 449 943
NcoI C'CATG_G
Cuts at: 0 443 1392
Size: 443 949
NgoMIV G'CCGG_C
Cuts at: 0 1215 1392
Size: 1215 177
NspI r_CATG'y
Cuts at: 0 910 1392
Size: 910 482
PspOMI G'GGCC_C
Cuts at: 0 214 1392
Size: 214 1178
PspXI vC'TCGA_Gb
Cuts at: 0 1336 1392
Size: 1336 56
PstI C_TGCA'G
Cuts at: 0 509 1392
Size: 509 883
PvuII CAG'CTG
Cuts at: 0 1054 1392
Size: 1054 338
SacI G_AGCT'C
Cuts at: 0 1337 1392
Size: 1337 55
SexAI A'CCwGG_T
Cuts at: 0 778 1392
Size: 778 614
SfcI C'TryA_G
Cuts at: 0 505 1392
Size: 505 887
SfoI GGC'GCC
Cuts at: 0 450 1392
Size: 450 942
SmlI C'TyrA_G
Cuts at: 0 289 1336 1392
Size: 289 1047 56
Fragments arranged by size:
1047 289 56
SphI G_CATG'C
Cuts at: 0 910 1392
Size: 910 482
StuI AGG'CCT
Cuts at: 0 496 526 1043 1392
Size: 496 30 517 349
Fragments arranged by size:
517 496 349 30
StyI C'CwwG_G
Cuts at: 0 443 1392
Size: 443 949
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 93 1270 1392
Size: 93 1177 122
Fragments arranged by size:
1177 122 93
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 336 507 1392
Size: 336 171 885
Fragments arranged by size:
885 336 171
XhoI C'TCGA_G
Cuts at: 0 1336 1392
Size: 1336 56
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlfI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BglI BglII Bme1580I BmgBI BplI BpmI BpuEI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI
BstXI BstYI BtgI BtgZI BtsI EaeI EagI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII
Hin4I HincII KasI KpnI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PspOMI PspXI PstI PvuII
SacI SexAI SfcI SfoI SmlI SphI StuI StyI
TaqII XcmI XhoI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI
BfrBI BlpI BmrI BmtI Bpu10I BsaI BsaAI BsaBI
BsiWI BspEI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EarI
EcoRV FspI FspAI HindIII HpaI MfeI MluI MmeI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TatI TstI Tth111I XbaI XmaI XmnI
ZraI