Restriction map for
pFN21AB8030
(Linear) (Six-base) MAPSORT of: KIBB8030.seq Check: 4880 from: 1 to: 1686
KIBB8030.orf
With 182 enzymes: SgfI *
November 15, 2011 13:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 331 1686
Size: 328 3 1355
Fragments arranged by size:
1355 328 3
AflIII A'CryG_T
Cuts at: 0 1348 1659 1686
Size: 1348 311 27
Fragments arranged by size:
1348 311 27
AlwNI CAG_nnn'CTG
Cuts at: 0 1385 1686
Size: 1385 301
ApoI r'AATT_y
Cuts at: 0 27 138 811 1248 1370 1686
Size: 27 111 673 437 122 316
Fragments arranged by size:
673 437 316 122 111 27
BamHI G'GATC_C
Cuts at: 0 1473 1686
Size: 1473 213
BbsI GAAGACnn'nnnn_
Cuts at: 0 61 593 1493 1686
Size: 61 532 900 193
Fragments arranged by size:
900 532 193 61
BbvCI CC'TCA_GC
Cuts at: 0 147 1686
Size: 147 1539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1600 1634 1686
Size: 1600 34 52
Fragments arranged by size:
1600 52 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1212 1686
Size: 1212 474
BglI GCCn_nnn'nGGC
Cuts at: 0 925 1686
Size: 925 761
Bme1580I G_kGCm'C
Cuts at: 0 7 1686
Size: 7 1679
BmrI ACTGGGnnnn_n'
Cuts at: 0 1086 1174 1448 1686
Size: 1086 88 274 238
Fragments arranged by size:
1086 274 238 88
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 416 1238 1686
Size: 416 822 448
Fragments arranged by size:
822 448 416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1655 1686
Size: 1655 31
Bpu10I CC'TnA_GC
Cuts at: 0 147 1686
Size: 147 1539
BsaBI GATnn'nnATC
Cuts at: 0 1361 1686
Size: 1361 325
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 854 884 1686
Size: 854 30 802
Fragments arranged by size:
854 802 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1399 1686
Size: 1399 287
BseYI C'CCAG_C
Cuts at: 0 842 1686
Size: 842 844
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 1686
Size: 601 1085
BsmI GAATG_Cn'
Cuts at: 0 1560 1686
Size: 1560 126
Bsp1286I G_dGCh'C
Cuts at: 0 7 1686
Size: 7 1679
BsrBI CCG'CTC
Cuts at: 0 1407 1686
Size: 1407 279
BsrDI GCAATG_nn'
Cuts at: 0 1298 1686
Size: 1298 388
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1051 1686
Size: 1051 635
BstEII G'GTnAC_C
Cuts at: 0 1341 1686
Size: 1341 345
BstYI r'GATC_y
Cuts at: 0 1473 1686
Size: 1473 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 214 1458 1686
Size: 214 1244 228
Fragments arranged by size:
1244 228 214
BtsI GCAGTG_nn'
Cuts at: 0 1202 1686
Size: 1202 484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1450 1485 1686
Size: 1450 35 201
Fragments arranged by size:
1450 201 35
DraI TTT'AAA
Cuts at: 0 606 701 1686
Size: 606 95 985
Fragments arranged by size:
985 606 95
DraIII CAC_nnn'GTG
Cuts at: 0 1182 1686
Size: 1182 504
EarI CTCTTCn'nnn_
Cuts at: 0 295 350 385 989 1686
Size: 295 55 35 604 697
Fragments arranged by size:
697 604 295 55 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1296 1686
Size: 1296 390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 331 1655 1686
Size: 328 3 1324 31
Fragments arranged by size:
1324 328 31 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 294 1686
Size: 294 1392
EcoRI G'AATT_C
Cuts at: 0 27 1248 1686
Size: 27 1221 438
Fragments arranged by size:
1221 438 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 844 1283 1686
Size: 844 439 403
Fragments arranged by size:
844 439 403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 75 107 1686
Size: 75 32 1579
Fragments arranged by size:
1579 75 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 804 1686
Size: 532 272 882
Fragments arranged by size:
882 532 272
MslI CAynn'nnrTG
Cuts at: 0 1079 1229 1686
Size: 1079 150 457
Fragments arranged by size:
1079 457 150
MspA1I CmG'CkG
Cuts at: 0 151 1385 1649 1686
Size: 151 1234 264 37
Fragments arranged by size:
1234 264 151 37
NspI r_CATG'y
Cuts at: 0 1352 1663 1686
Size: 1352 311 23
Fragments arranged by size:
1352 311 23
PciI A'CATG_T
Cuts at: 0 1348 1659 1686
Size: 1348 311 27
Fragments arranged by size:
1348 311 27
PfoI T'CCnGG_A
Cuts at: 0 1252 1686
Size: 1252 434
PvuII CAG'CTG
Cuts at: 0 151 1385 1686
Size: 151 1234 301
Fragments arranged by size:
1234 301 151
SexAI A'CCwGG_T
Cuts at: 0 1453 1686
Size: 1453 233
SspI AAT'ATT
Cuts at: 0 755 1686
Size: 755 931
StyI C'CwwG_G
Cuts at: 0 116 1686
Size: 116 1570
SwaI ATTT'AAAT
Cuts at: 0 701 1686
Size: 701 985
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1193 1628 1686
Size: 1193 435 58
Fragments arranged by size:
1193 435 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1541 1573 1686
Size: 1541 32 113
Fragments arranged by size:
1541 113 32
Tth111I GACn'n_nGTC
Cuts at: 0 1492 1686
Size: 1492 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 264 1146 1686
Size: 264 882 540
Fragments arranged by size:
882 540 264
Enzymes that do cut:
AcuI AflIII AlwNI ApoI BamHI BbsI BbvCI BcgI
BciVI BglI Bme1580I BmrI BplI BpmI Bpu10I BsaBI
BsaXI BseRI BseYI BsgI BsmI Bsp1286I BsrBI BsrDI
BstAPI BstEII BstYI BtgZI BtsI CspCI DraI DraIII
EarI EciI Eco57MI EcoNI EcoRI FalI Hin4I MmeI
MslI MspA1I NspI PciI PfoI PvuII SexAI SspI
StyI SwaI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BanII BbeI BclI
BfrBI BglII BlpI BmgBI BmtI BpuEI BsaI BsaAI
BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BspMI BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I
Bsu36I BtgI ClaI DrdI EaeI EagI EcoICRI EcoO109I
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
TatI XbaI XhoI XmaI XmnI ZraI