(Linear) (Six-base) MAPSORT of: KIBB8024.seq  Check: 3614  from: 1  to: 615



KIBB8024.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AccI GT'mk_AC

Cuts at:      0     401     615 
   Size:        401     214
 
AlwNI CAG_nnn'CTG

Cuts at:      0     315     615 
   Size:        315     300
 
ApoI r'AATT_y

Cuts at:      0     421     440     572     615 
   Size:        421      19     132      43

  Fragments arranged by size: 

                 421     132      43      19
 
BanII G_rGCy'C

Cuts at:      0     252     615 
   Size:        252     363
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     216     569     615 
   Size:        216     353      46

  Fragments arranged by size: 

                 353     216      46
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     260     615 
   Size:        260     355
 
BsiHKAI G_wGCw'C

Cuts at:      0     583     615 
   Size:        583      32
 
BsmI GAATG_Cn'

Cuts at:      0     474     615 
   Size:        474     141
 
Bsp1286I G_dGCh'C

Cuts at:      0     252     583     615 
   Size:        252     331      32

  Fragments arranged by size: 

                 331     252      32
 
BspHI T'CATG_A

Cuts at:      0     494     615 
   Size:        494     121
 
BstBI TT'CG_AA

Cuts at:      0     219     615 
   Size:        219     396
 
BtgI C'CryG_G

Cuts at:      0     252     615 
   Size:        252     363
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     116     151     615 
   Size:        116      35     464

  Fragments arranged by size: 

                 464     116      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     216     569     615 
   Size:        216     353      46

  Fragments arranged by size: 

                 353     216      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     529     615 
   Size:        529      86
 
EcoRI G'AATT_C

Cuts at:      0     440     615 
   Size:        440     175
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     274     306     615 
   Size:        274      32     309

  Fragments arranged by size: 

                 309     274      32
 
HincII GTy'rAC

Cuts at:      0     351     615 
   Size:        351     264
 
HindIII A'AGCT_T

Cuts at:      0     181     615 
   Size:        181     434
 
MfeI C'AATT_G

Cuts at:      0     128     615 
   Size:        128     487
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      45     615 
   Size:         45     570
 
MslI CAynn'nnrTG

Cuts at:      0     265     499     615 
   Size:        265     234     116

  Fragments arranged by size: 

                 265     234     116
 
MspA1I CmG'CkG

Cuts at:      0     508     544     615 
   Size:        508      36      71

  Fragments arranged by size: 

                 508      71      36
 
NcoI C'CATG_G

Cuts at:      0     252     615 
   Size:        252     363
 
PfoI T'CCnGG_A

Cuts at:      0     531     615 
   Size:        531      84
 
PpuMI rG'GwC_Cy

Cuts at:      0     529     615 
   Size:        529      86
 
PstI C_TGCA'G

Cuts at:      0     320     615 
   Size:        320     295
 
PvuII CAG'CTG

Cuts at:      0     508     544     615 
   Size:        508      36      71

  Fragments arranged by size: 

                 508      71      36
 
SanDI GG'GwC_CC

Cuts at:      0     529     615 
   Size:        529      86
 
SfcI C'TryA_G

Cuts at:      0     155     316     615 
   Size:        155     161     299

  Fragments arranged by size: 

                 299     161     155
 
StyI C'CwwG_G

Cuts at:      0     252     435     615 
   Size:        252     183     180

  Fragments arranged by size: 

                 252     183     180
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     360     392     615 
   Size:        360      32     223

  Fragments arranged by size: 

                 360     223      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     215     615 
   Size:        215     400
 

 Enzymes that do cut:

     AccI    AlwNI     ApoI    BanII     BpmI    BseRI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BstBI     BtgI    CspCI  Eco57MI EcoO109I    EcoRI
    Hin4I   HincII  HindIII     MfeI     MmeI     MslI   MspA1I     NcoI
     PfoI    PpuMI     PstI    PvuII    SanDI     SfcI     StyI     TstI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MluI     MscI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI