Restriction map for
pFN21AB8007
(Linear) (Six-base) MAPSORT of: KIBB8007.seq Check: 5028 from: 1 to: 2766
KIBB8007.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 812 935 2236 2341 2766
Size: 503 309 123 1301 105 425
Fragments arranged by size:
1301 503 425 309 123 105
AflIII A'CryG_T
Cuts at: 0 2665 2766
Size: 2665 101
ApoI r'AATT_y
Cuts at: 0 235 1254 2104 2766
Size: 235 1019 850 662
Fragments arranged by size:
1019 850 662 235
AseI AT'TA_AT
Cuts at: 0 1946 2766
Size: 1946 820
AvaI C'yCGr_G
Cuts at: 0 2297 2766
Size: 2297 469
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 767 800 2766
Size: 767 33 1966
Fragments arranged by size:
1966 767 33
BamHI G'GATC_C
Cuts at: 0 1008 2766
Size: 1008 1758
BanII G_rGCy'C
Cuts at: 0 2591 2766
Size: 2591 175
BclI T'GATC_A
Cuts at: 0 1545 2766
Size: 1545 1221
BglI GCCn_nnn'nGGC
Cuts at: 0 541 2766
Size: 541 2225
BlpI GC'TnA_GC
Cuts at: 0 2585 2766
Size: 2585 181
BmrI ACTGGGnnnn_n'
Cuts at: 0 1440 2766
Size: 1440 1326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 827 1136 2766
Size: 764 63 309 1630
Fragments arranged by size:
1630 764 309 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1451 1794 2766
Size: 1451 343 972
Fragments arranged by size:
1451 972 343
BsaI GGTCTCn'nnnn_
Cuts at: 0 1128 2766
Size: 1128 1638
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 669 699 762 792 2766
Size: 669 30 63 30 1974
Fragments arranged by size:
1974 669 63 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1791 1817 2263 2315 2766
Size: 1791 26 446 52 451
Fragments arranged by size:
1791 451 446 52 26
BseYI C'CCAG_C
Cuts at: 0 1382 1502 2766
Size: 1382 120 1264
Fragments arranged by size:
1382 1264 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 920 2766
Size: 920 1846
BsiHKAI G_wGCw'C
Cuts at: 0 2591 2766
Size: 2591 175
BsmI GAATG_Cn'
Cuts at: 0 1038 2534 2766
Size: 1038 1496 232
Fragments arranged by size:
1496 1038 232
BsmBI CGTCTCn'nnnn_
Cuts at: 0 656 2766
Size: 656 2110
Bsp1286I G_dGCh'C
Cuts at: 0 2591 2766
Size: 2591 175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2566 2766
Size: 2566 200
BsrDI GCAATG_nn'
Cuts at: 0 1590 2766
Size: 1590 1176
BssSI C'ACGA_G
Cuts at: 0 1520 2766
Size: 1520 1246
BstEII G'GTnAC_C
Cuts at: 0 2648 2766
Size: 2648 118
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1061 1382 1502 2238 2766
Size: 1061 321 120 736 528
Fragments arranged by size:
1061 736 528 321 120
BstYI r'GATC_y
Cuts at: 0 837 1008 1077 1455 1575 2682 2766
Size: 837 171 69 378 120 1107 84
Fragments arranged by size:
1107 837 378 171 120 84 69
BtgI C'CryG_G
Cuts at: 0 1238 1994 2766
Size: 1238 756 772
Fragments arranged by size:
1238 772 756
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 238 2766
Size: 238 2528
BtsI GCAGTG_nn'
Cuts at: 0 5 2766
Size: 5 2761
ClaI AT'CG_AT
Cuts at: 0 2021 2766
Size: 2021 745
DraI TTT'AAA
Cuts at: 0 263 2766
Size: 263 2503
EaeI y'GGCC_r
Cuts at: 0 2 2766
Size: 2 2764
EarI CTCTTCn'nnn_
Cuts at: 0 1161 1179 2360 2735 2766
Size: 1161 18 1181 375 31
Fragments arranged by size:
1181 1161 375 31 18
EcoICRI GAG'CTC
Cuts at: 0 2589 2766
Size: 2589 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 764 812 827 935 1136 2236 2341
Size: 503 261 48 15 108 201 1100 105
Cuts at: 2341 2766
Size: 425
Fragments arranged by size:
1100 503 425 261 201 108 105 48
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2573 2766
Size: 2573 193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1831 2344 2766
Size: 1831 513 422
Fragments arranged by size:
1831 513 422
HaeII r_GCGC'y
Cuts at: 0 1785 2766
Size: 1785 981
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 748 780 2074 2106 2766
Size: 748 32 1294 32 660
Fragments arranged by size:
1294 748 660 32 32
HincII GTy'rAC
Cuts at: 0 2178 2421 2766
Size: 2178 243 345
Fragments arranged by size:
2178 345 243
HindIII A'AGCT_T
Cuts at: 0 1648 2321 2766
Size: 1648 673 445
Fragments arranged by size:
1648 673 445
MfeI C'AATT_G
Cuts at: 0 1285 2766
Size: 1285 1481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 1767 2766
Size: 490 1277 999
Fragments arranged by size:
1277 999 490
MscI TGG'CCA
Cuts at: 0 4 2766
Size: 4 2762
MslI CAynn'nnrTG
Cuts at: 0 506 1059 2766
Size: 506 553 1707
Fragments arranged by size:
1707 553 506
MspA1I CmG'CkG
Cuts at: 0 1230 1278 2766
Size: 1230 48 1488
Fragments arranged by size:
1488 1230 48
NcoI C'CATG_G
Cuts at: 0 1238 1994 2766
Size: 1238 756 772
Fragments arranged by size:
1238 772 756
NspI r_CATG'y
Cuts at: 0 2412 2669 2766
Size: 2412 257 97
Fragments arranged by size:
2412 257 97
PciI A'CATG_T
Cuts at: 0 2665 2766
Size: 2665 101
PsiI TTA'TAA
Cuts at: 0 2111 2766
Size: 2111 655
PstI C_TGCA'G
Cuts at: 0 457 1350 1925 2766
Size: 457 893 575 841
Fragments arranged by size:
893 841 575 457
SacI G_AGCT'C
Cuts at: 0 2591 2766
Size: 2591 175
ScaI AGT'ACT
Cuts at: 0 825 2371 2766
Size: 825 1546 395
Fragments arranged by size:
1546 825 395
SfcI C'TryA_G
Cuts at: 0 453 1346 1921 2365 2766
Size: 453 893 575 444 401
Fragments arranged by size:
893 575 453 444 401
SmlI C'TyrA_G
Cuts at: 0 1466 1773 2297 2766
Size: 1466 307 524 469
Fragments arranged by size:
1466 524 469 307
SpeI A'CTAG_T
Cuts at: 0 28 820 2766
Size: 28 792 1946
Fragments arranged by size:
1946 792 28
SphI G_CATG'C
Cuts at: 0 2412 2766
Size: 2412 354
SspI AAT'ATT
Cuts at: 0 1796 2235 2766
Size: 1796 439 531
Fragments arranged by size:
1796 531 439
StyI C'CwwG_G
Cuts at: 0 16 365 1238 1994 2766
Size: 16 349 873 756 772
Fragments arranged by size:
873 772 756 349 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 989 2766
Size: 989 1777
TatI w'GTAC_w
Cuts at: 0 823 2165 2304 2369 2766
Size: 823 1342 139 65 397
Fragments arranged by size:
1342 823 397 139 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 762 794 2766
Size: 762 32 1972
Fragments arranged by size:
1972 762 32
XbaI T'CTAG_A
Cuts at: 0 952 2766
Size: 952 1814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 903 2766
Size: 903 1863
XhoI C'TCGA_G
Cuts at: 0 2297 2766
Size: 2297 469
XmnI GAAnn'nnTTC
Cuts at: 0 103 884 960 2348 2723 2766
Size: 103 781 76 1388 375 43
Fragments arranged by size:
1388 781 375 103 76 43
Enzymes that do cut:
AcuI AflIII ApoI AseI AvaI BaeI BamHI BanII
BclI BglI BlpI BmrI BpmI BpuEI BsaI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrDI BssSI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI DraI EaeI EarI EcoICRI Eco57MI EcoNI FalI
HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NcoI NspI PciI PsiI PstI SacI ScaI
SfcI SmlI SpeI SphI SspI StyI TaqII TatI
TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvrII BanI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglII Bme1580I BmgBI BmtI BplI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I CspCI
DraIII DrdI EagI EciI EcoO109I EcoRI EcoRV FseI
FspI FspAI HpaI KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SrfI StuI SwaI Tth111I XmaI ZraI