Restriction map for
pFN21AB7993
(Linear) (Six-base) MAPSORT of: KIBB7993.seq Check: 7950 from: 1 to: 1407
KIBB7993.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 456 1407
Size: 456 951
Acc65I G'GTAC_C
Cuts at: 0 845 1407
Size: 845 562
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 857 1375 1407
Size: 610 247 518 32
Fragments arranged by size:
610 518 247 32
AleI CACnn'nnGTG
Cuts at: 0 730 1407
Size: 730 677
AlwNI CAG_nnn'CTG
Cuts at: 0 213 568 951 1407
Size: 213 355 383 456
Fragments arranged by size:
456 383 355 213
ApaI G_GGCC'C
Cuts at: 0 172 1407
Size: 172 1235
ApoI r'AATT_y
Cuts at: 0 1248 1407
Size: 1248 159
AvaI C'yCGr_G
Cuts at: 0 51 1294 1407
Size: 51 1243 113
Fragments arranged by size:
1243 113 51
BanI G'GyrC_C
Cuts at: 0 61 127 178 845 1407
Size: 61 66 51 667 562
Fragments arranged by size:
667 562 66 61 51
BanII G_rGCy'C
Cuts at: 0 82 172 1352 1407
Size: 82 90 1180 55
Fragments arranged by size:
1180 90 82 55
BbeI G_GCGC'C
Cuts at: 0 65 131 182 1407
Size: 65 66 51 1225
Fragments arranged by size:
1225 66 65 51
BbvCI CC'TCA_GC
Cuts at: 0 785 830 1016 1407
Size: 785 45 186 391
Fragments arranged by size:
785 391 186 45
BglI GCCn_nnn'nGGC
Cuts at: 0 95 119 176 1407
Size: 95 24 57 1231
Fragments arranged by size:
1231 95 57 24
BglII A'GATC_T
Cuts at: 0 716 1407
Size: 716 691
BlpI GC'TnA_GC
Cuts at: 0 39 1407
Size: 39 1368
Bme1580I G_kGCm'C
Cuts at: 0 172 1401 1407
Size: 172 1229 6
Fragments arranged by size:
1229 172 6
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 101 1407
Size: 101 1306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 355 376 505 652 853 1207 1232
Size: 235 120 21 129 147 201 354 25
Cuts at: 1232 1314 1407
Size: 82 93
Fragments arranged by size:
354 235 201 147 129 120 93 82
25 21
Bpu10I CC'TnA_GC
Cuts at: 0 76 785 830 1016 1321 1407
Size: 76 709 45 186 305 86
Fragments arranged by size:
709 305 186 86 76 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1024 1407
Size: 475 549 383
Fragments arranged by size:
549 475 383
BsaI GGTCTCn'nnnn_
Cuts at: 0 352 856 1407
Size: 352 504 551
Fragments arranged by size:
551 504 352
BsaHI Gr'CG_yC
Cuts at: 0 62 128 179 236 1407
Size: 62 66 51 57 1171
Fragments arranged by size:
1171 66 62 57 51
BsaWI w'CCGG_w
Cuts at: 0 902 1370 1407
Size: 902 468 37
Fragments arranged by size:
902 468 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 31 349 373 379 403 742 772
Size: 1 30 318 24 6 24 339 30
Cuts at: 772 1189 1205 1219 1235 1407
Size: 417 16 14 16 172
Fragments arranged by size:
417 339 318 172 30 30 24 24
16 16 14 6 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 820 1033 1078 1210 1407
Size: 283 537 213 45 132 197
Fragments arranged by size:
537 283 213 197 132 45
BseYI C'CCAG_C
Cuts at: 0 880 1407
Size: 880 527
BsiEI CG_ry'CG
Cuts at: 0 139 1407
Size: 139 1268
BsiHKAI G_wGCw'C
Cuts at: 0 82 1407
Size: 82 1325
BsmBI CGTCTCn'nnnn_
Cuts at: 0 682 1407
Size: 682 725
Bsp1286I G_dGCh'C
Cuts at: 0 82 172 1352 1401 1407
Size: 82 90 1180 49 6
Fragments arranged by size:
1180 90 82 49 6
BspEI T'CCGG_A
Cuts at: 0 902 1370 1407
Size: 902 468 37
Fragments arranged by size:
902 468 37
BspHI T'CATG_A
Cuts at: 0 1133 1407
Size: 1133 274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 456 1407
Size: 456 951
BsrBI CCG'CTC
Cuts at: 0 85 1407
Size: 85 1322
BsrDI GCAATG_nn'
Cuts at: 0 1004 1320 1407
Size: 1004 316 87
Fragments arranged by size:
1004 316 87
BsrFI r'CCGG_y
Cuts at: 0 181 1202 1407
Size: 181 1021 205
Fragments arranged by size:
1021 205 181
BssSI C'ACGA_G
Cuts at: 0 1049 1407
Size: 1049 358
BstEII G'GTnAC_C
Cuts at: 0 195 1407
Size: 195 1212
BstXI CCAn_nnnn'nTGG
Cuts at: 0 436 1407
Size: 436 971
BstYI r'GATC_y
Cuts at: 0 716 1407
Size: 716 691
Bsu36I CC'TnA_GG
Cuts at: 0 673 1407
Size: 673 734
BtgI C'CryG_G
Cuts at: 0 57 743 1304 1407
Size: 57 686 561 103
Fragments arranged by size:
686 561 103 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 765 1407
Size: 765 642
BtsI GCAGTG_nn'
Cuts at: 0 460 1275 1341 1407
Size: 460 815 66 66
Fragments arranged by size:
815 460 66 66
EaeI y'GGCC_r
Cuts at: 0 136 146 392 812 1407
Size: 136 10 246 420 595
Fragments arranged by size:
595 420 246 136 10
EagI C'GGCC_G
Cuts at: 0 136 1407
Size: 136 1271
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 505 1371 1407
Size: 505 866 36
Fragments arranged by size:
866 505 36
EcoICRI GAG'CTC
Cuts at: 0 80 1407
Size: 80 1327
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 355 376 505 610 652 853 857
Size: 235 120 21 129 105 42 201 4
Cuts at: 857 1207 1232 1314 1375 1407
Size: 350 25 82 61 32
Fragments arranged by size:
350 235 201 129 120 105 82 61
42 32 25 21 4
EcoO109I rG'GnC_Cy
Cuts at: 0 168 677 1342 1407
Size: 168 509 665 65
Fragments arranged by size:
665 509 168 65
EcoRI G'AATT_C
Cuts at: 0 1248 1407
Size: 1248 159
HaeII r_GCGC'y
Cuts at: 0 65 131 182 1039 1407
Size: 65 66 51 857 368
Fragments arranged by size:
857 368 66 65 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 694 726 1407
Size: 373 32 289 32 681
Fragments arranged by size:
681 373 289 32 32
HincII GTy'rAC
Cuts at: 0 698 1407
Size: 698 709
KasI G'GCGC_C
Cuts at: 0 61 127 178 1407
Size: 61 66 51 1229
Fragments arranged by size:
1229 66 61 51
KpnI G_GTAC'C
Cuts at: 0 849 1407
Size: 849 558
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 974 1407
Size: 746 228 433
Fragments arranged by size:
746 433 228
MscI TGG'CCA
Cuts at: 0 814 1407
Size: 814 593
MslI CAynn'nnrTG
Cuts at: 0 730 1138 1407
Size: 730 408 269
Fragments arranged by size:
730 408 269
MspA1I CmG'CkG
Cuts at: 0 59 225 255 565 1086 1113 1407
Size: 59 166 30 310 521 27 294
Fragments arranged by size:
521 310 294 166 59 30 27
NaeI GCC'GGC
Cuts at: 0 183 1407
Size: 183 1224
NarI GG'CG_CC
Cuts at: 0 62 128 179 1407
Size: 62 66 51 1228
Fragments arranged by size:
1228 66 62 51
NcoI C'CATG_G
Cuts at: 0 743 1304 1407
Size: 743 561 103
Fragments arranged by size:
743 561 103
NgoMIV G'CCGG_C
Cuts at: 0 181 1407
Size: 181 1226
NotI GC'GGCC_GC
Cuts at: 0 136 1407
Size: 136 1271
NspI r_CATG'y
Cuts at: 0 507 1407
Size: 507 900
PpuMI rG'GwC_Cy
Cuts at: 0 677 1342 1407
Size: 677 665 65
Fragments arranged by size:
677 665 65
PshAI GACnn'nnGTC
Cuts at: 0 752 1407
Size: 752 655
PspOMI G'GGCC_C
Cuts at: 0 168 1407
Size: 168 1239
PstI C_TGCA'G
Cuts at: 0 380 467 710 956 1085 1407
Size: 380 87 243 246 129 322
Fragments arranged by size:
380 322 246 243 129 87
PvuII CAG'CTG
Cuts at: 0 565 1113 1407
Size: 565 548 294
Fragments arranged by size:
565 548 294
RsrII CG'GwC_CG
Cuts at: 0 1373 1407
Size: 1373 34
SacI G_AGCT'C
Cuts at: 0 82 1407
Size: 82 1325
SacII CC_GC'GG
Cuts at: 0 60 1407
Size: 60 1347
SfcI C'TryA_G
Cuts at: 0 376 463 706 952 1081 1407
Size: 376 87 243 246 129 326
Fragments arranged by size:
376 326 246 243 129 87
SfoI GGC'GCC
Cuts at: 0 63 129 180 1407
Size: 63 66 51 1227
Fragments arranged by size:
1227 66 63 51
SmlI C'TyrA_G
Cuts at: 0 454 1039 1407
Size: 454 585 368
Fragments arranged by size:
585 454 368
SphI G_CATG'C
Cuts at: 0 507 1407
Size: 507 900
StyI C'CwwG_G
Cuts at: 0 99 638 743 1304 1407
Size: 99 539 105 561 103
Fragments arranged by size:
561 539 105 103 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1 33 349 381 1205 1237 1407
Size: 1 32 316 32 824 32 170
Fragments arranged by size:
824 316 170 32 32 32 1
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 877 1098 1407
Size: 877 221 309
Fragments arranged by size:
877 309 221
Enzymes that do cut:
AarI Acc65I AcuI AleI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbvCI BglI BglII BlpI Bme1580I
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EagI EciI EcoICRI
Eco57MI EcoO109I EcoRI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PpuMI PshAI PspOMI PstI PvuII RsrII
SacI SacII SfcI SfoI SmlI SphI StyI TstI
XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BcgI BciVI BclI BfrBI BmgBI BmrI
BmtI BsaAI BsaBI BsgI BsiWI BsmI BsrGI BssHII
BstAPI BstBI BstZ17I ClaI CspCI DraI DraIII DrdI
EarI EcoNI EcoRV FalI FseI FspI FspAI HindIII
HpaI MfeI MluI NdeI NheI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI
XmaI XmnI ZraI