Restriction map for
pFN21AB7989
(Linear) (Six-base) MAPSORT of: KIBB7989.seq Check: 2139 from: 1 to: 1326
KIBB7989.orf
With 182 enzymes: SgfI *
July 3, 2012 16:32 ..
AleI CACnn'nnGTG
Cuts at: 0 403 1326
Size: 403 923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1045 1326
Size: 1045 281
ApaI G_GGCC'C
Cuts at: 0 66 1326
Size: 66 1260
ApaLI G'TGCA_C
Cuts at: 0 7 913 1326
Size: 7 906 413
Fragments arranged by size:
906 413 7
ApoI r'AATT_y
Cuts at: 0 145 1326
Size: 145 1181
AvaI C'yCGr_G
Cuts at: 0 742 923 1002 1326
Size: 742 181 79 324
Fragments arranged by size:
742 324 181 79
BanI G'GyrC_C
Cuts at: 0 102 204 261 351 1326
Size: 102 102 57 90 975
Fragments arranged by size:
975 102 102 90 57
BanII G_rGCy'C
Cuts at: 0 66 842 1130 1326
Size: 66 776 288 196
Fragments arranged by size:
776 288 196 66
BbeI G_GCGC'C
Cuts at: 0 106 208 265 1326
Size: 106 102 57 1061
Fragments arranged by size:
1061 106 102 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 642 1326
Size: 642 684
BclI T'GATC_A
Cuts at: 0 551 1326
Size: 551 775
BglI GCCn_nnn'nGGC
Cuts at: 0 61 298 623 669 1326
Size: 61 237 325 46 657
Fragments arranged by size:
657 325 237 61 46
BglII A'GATC_T
Cuts at: 0 1049 1326
Size: 1049 277
BlpI GC'TnA_GC
Cuts at: 0 682 1326
Size: 682 644
Bme1580I G_kGCm'C
Cuts at: 0 11 66 410 566 665 917 1326
Size: 11 55 344 156 99 252 409
Fragments arranged by size:
409 344 252 156 99 55 11
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1144 1249 1326
Size: 517 627 105 77
Fragments arranged by size:
627 517 105 77
Bpu10I CC'TnA_GC
Cuts at: 0 1079 1326
Size: 1079 247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1326
Size: 1222 104
BsaHI Gr'CG_yC
Cuts at: 0 103 205 212 262 1326
Size: 103 102 7 50 1064
Fragments arranged by size:
1064 103 102 50 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 31 896 926 1326
Size: 1 30 865 30 400
Fragments arranged by size:
865 400 30 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 275 346 1326
Size: 275 71 980
Fragments arranged by size:
980 275 71
BseYI C'CCAG_C
Cuts at: 0 42 957 1095 1110 1326
Size: 42 915 138 15 216
Fragments arranged by size:
915 216 138 42 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 742 997 1326
Size: 403 339 255 329
Fragments arranged by size:
403 339 329 255
BsiEI CG_ry'CG
Cuts at: 0 128 669 1326
Size: 128 541 657
Fragments arranged by size:
657 541 128
BsiHKAI G_wGCw'C
Cuts at: 0 11 917 1326
Size: 11 906 409
Fragments arranged by size:
906 409 11
BsmBI CGTCTCn'nnnn_
Cuts at: 0 80 940 1326
Size: 80 860 386
Fragments arranged by size:
860 386 80
Bsp1286I G_dGCh'C
Cuts at: 0 11 66 410 566 665 842 917 1130
Size: 11 55 344 156 99 177 75 213
Cuts at: 1130 1326
Size: 196
Fragments arranged by size:
344 213 196 177 156 99 75 55
11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 512 854 1326
Size: 512 342 472
Fragments arranged by size:
512 472 342
BsrBI CCG'CTC
Cuts at: 0 111 1088 1326
Size: 111 977 238
Fragments arranged by size:
977 238 111
BsrDI GCAATG_nn'
Cuts at: 0 518 1326
Size: 518 808
BsrFI r'CCGG_y
Cuts at: 0 55 99 123 668 806 1326
Size: 55 44 24 545 138 520
Fragments arranged by size:
545 520 138 55 44 24
BssHII G'CGCG_C
Cuts at: 0 437 1326
Size: 437 889
BssSI C'ACGA_G
Cuts at: 0 328 1326
Size: 328 998
BstYI r'GATC_y
Cuts at: 0 1049 1326
Size: 1049 277
Bsu36I CC'TnA_GG
Cuts at: 0 287 1326
Size: 287 1039
BtgI C'CryG_G
Cuts at: 0 23 1170 1326
Size: 23 1147 156
Fragments arranged by size:
1147 156 23
BtsI GCAGTG_nn'
Cuts at: 0 398 676 1205 1272 1281 1326
Size: 398 278 529 67 9 45
Fragments arranged by size:
529 398 278 67 45 9
EaeI y'GGCC_r
Cuts at: 0 125 428 605 666 855 1326
Size: 125 303 177 61 189 471
Fragments arranged by size:
471 303 189 177 125 61
EagI C'GGCC_G
Cuts at: 0 125 666 1326
Size: 125 541 660
Fragments arranged by size:
660 541 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 9 1326
Size: 9 1317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1144 1249 1326
Size: 517 627 105 77
Fragments arranged by size:
627 517 105 77
EcoO109I rG'GnC_Cy
Cuts at: 0 62 243 501 1326
Size: 62 181 258 825
Fragments arranged by size:
825 258 181 62
EcoRI G'AATT_C
Cuts at: 0 145 1326
Size: 145 1181
HaeII r_GCGC'y
Cuts at: 0 33 106 208 265 1326
Size: 33 73 102 57 1061
Fragments arranged by size:
1061 102 73 57 33
KasI G'GCGC_C
Cuts at: 0 102 204 261 1326
Size: 102 102 57 1065
Fragments arranged by size:
1065 102 102 57
MscI TGG'CCA
Cuts at: 0 607 1326
Size: 607 719
MslI CAynn'nnrTG
Cuts at: 0 403 1326
Size: 403 923
MspA1I CmG'CkG
Cuts at: 0 52 136 189 726 853 970 1095 1326
Size: 52 84 53 537 127 117 125 231
Fragments arranged by size:
537 231 127 125 117 84 53 52
NaeI GCC'GGC
Cuts at: 0 57 101 125 670 1326
Size: 57 44 24 545 656
Fragments arranged by size:
656 545 57 44 24
NarI GG'CG_CC
Cuts at: 0 103 205 262 1326
Size: 103 102 57 1064
Fragments arranged by size:
1064 103 102 57
NcoI C'CATG_G
Cuts at: 0 23 1170 1326
Size: 23 1147 156
Fragments arranged by size:
1147 156 23
NdeI CA'TA_TG
Cuts at: 0 249 1326
Size: 249 1077
NgoMIV G'CCGG_C
Cuts at: 0 55 99 123 668 1326
Size: 55 44 24 545 658
Fragments arranged by size:
658 545 55 44 24
NspI r_CATG'y
Cuts at: 0 1032 1326
Size: 1032 294
PasI CC'CwG_GG
Cuts at: 0 369 1326
Size: 369 957
PfoI T'CCnGG_A
Cuts at: 0 1121 1326
Size: 1121 205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 937 969 1326
Size: 937 32 357
Fragments arranged by size:
937 357 32
PpuMI rG'GwC_Cy
Cuts at: 0 243 501 1326
Size: 243 258 825
Fragments arranged by size:
825 258 243
PspOMI G'GGCC_C
Cuts at: 0 62 1326
Size: 62 1264
PstI C_TGCA'G
Cuts at: 0 509 1326
Size: 509 817
PvuII CAG'CTG
Cuts at: 0 52 136 853 970 1326
Size: 52 84 717 117 356
Fragments arranged by size:
717 356 117 84 52
SanDI GG'GwC_CC
Cuts at: 0 243 1326
Size: 243 1083
SbfI CC_TGCA'GG
Cuts at: 0 509 1326
Size: 509 817
SfcI C'TryA_G
Cuts at: 0 505 1326
Size: 505 821
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 298 1326
Size: 298 1028
SfoI GGC'GCC
Cuts at: 0 104 206 263 1326
Size: 104 102 57 1063
Fragments arranged by size:
1063 104 102 57
SmaI CCC'GGG
Cuts at: 0 1004 1326
Size: 1004 322
SmlI C'TyrA_G
Cuts at: 0 1237 1326
Size: 1237 89
SphI G_CATG'C
Cuts at: 0 1032 1326
Size: 1032 294
StyI C'CwwG_G
Cuts at: 0 23 602 962 1170 1292 1326
Size: 23 579 360 208 122 34
Fragments arranged by size:
579 360 208 122 34 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1183 1326
Size: 1183 143
TatI w'GTAC_w
Cuts at: 0 698 1134 1326
Size: 698 436 192
Fragments arranged by size:
698 436 192
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1 33 896 928 1326
Size: 1 32 863 32 398
Fragments arranged by size:
863 398 32 32 1
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 615 1177 1326
Size: 615 562 149
Fragments arranged by size:
615 562 149
XmaI C'CCGG_G
Cuts at: 0 1002 1326
Size: 1002 324
Enzymes that do cut:
AleI AlfI ApaI ApaLI ApoI AvaI BanI BanII
BbeI BbsI BclI BglI BglII BlpI Bme1580I BpmI
Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstYI Bsu36I BtgI BtsI EaeI EagI EciI
Eco57MI EcoO109I EcoRI HaeII KasI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PfoI
PpiI PpuMI PspOMI PstI PvuII SanDI SbfI SfcI
SfiI SfoI SmaI SmlI SphI StyI TaqII TatI
TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AloI AlwNI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BmgBI
BmrI BmtI BplI BsaI BsaAI BsaBI BsaWI BsiWI
BsmI BspEI BspHI BsrGI BstAPI BstBI BstEII BstXI
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EarI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI ScaI SexAI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI
ZraI