Restriction map for
pFN21AB7962
(Linear) (Six-base) MAPSORT of: KIBB7962.seq Check: 7722 from: 1 to: 1191
KIBB7962.orf
With 182 enzymes: SgfI *
March 22, 2012 15:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 337 487 1191
Size: 337 150 704
Fragments arranged by size:
704 337 150
AccI GT'mk_AC
Cuts at: 0 458 1191
Size: 458 733
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1191
Size: 307 884
AfeI AGC'GCT
Cuts at: 0 701 1191
Size: 701 490
AhdI GACnn_n'nnGTC
Cuts at: 0 1017 1191
Size: 1017 174
AleI CACnn'nnGTG
Cuts at: 0 1114 1191
Size: 1114 77
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 413 1191
Size: 413 778
ApoI r'AATT_y
Cuts at: 0 961 1191
Size: 961 230
AvaI C'yCGr_G
Cuts at: 0 313 446 1191
Size: 313 133 745
Fragments arranged by size:
745 313 133
BanI G'GyrC_C
Cuts at: 0 14 148 658 1191
Size: 14 134 510 533
Fragments arranged by size:
533 510 134 14
BanII G_rGCy'C
Cuts at: 0 326 697 1056 1191
Size: 326 371 359 135
Fragments arranged by size:
371 359 326 135
BbeI G_GCGC'C
Cuts at: 0 152 1191
Size: 152 1039
BbsI GAAGACnn'nnnn_
Cuts at: 0 32 802 1191
Size: 32 770 389
Fragments arranged by size:
770 389 32
BbvCI CC'TCA_GC
Cuts at: 0 1038 1191
Size: 1038 153
BciVI GTATCCnnnnn_n'
Cuts at: 0 593 1191
Size: 593 598
BglI GCCn_nnn'nGGC
Cuts at: 0 139 413 1191
Size: 139 274 778
Fragments arranged by size:
778 274 139
BglII A'GATC_T
Cuts at: 0 230 1191
Size: 230 961
BlpI GC'TnA_GC
Cuts at: 0 187 595 1191
Size: 187 408 596
Fragments arranged by size:
596 408 187
Bme1580I G_kGCm'C
Cuts at: 0 402 661 1191
Size: 402 259 530
Fragments arranged by size:
530 402 259
BmgBI CAC'GTC
Cuts at: 0 1019 1191
Size: 1019 172
Bpu10I CC'TnA_GC
Cuts at: 0 466 1038 1191
Size: 466 572 153
Fragments arranged by size:
572 466 153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1191
Size: 874 317
BsaI GGTCTCn'nnnn_
Cuts at: 0 556 1191
Size: 556 635
BsaHI Gr'CG_yC
Cuts at: 0 149 1191
Size: 149 1042
BsaWI w'CCGG_w
Cuts at: 0 76 836 1153 1191
Size: 76 760 317 38
Fragments arranged by size:
760 317 76 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 58 88 1191
Size: 58 30 1103
Fragments arranged by size:
1103 58 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 1191
Size: 118 1073
BseYI C'CCAG_C
Cuts at: 0 51 152 696 702 1191
Size: 51 101 544 6 489
Fragments arranged by size:
544 489 101 51 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 685 1191
Size: 409 276 506
Fragments arranged by size:
506 409 276
BsiEI CG_ry'CG
Cuts at: 0 165 1191
Size: 165 1026
BsiHKAI G_wGCw'C
Cuts at: 0 326 1056 1191
Size: 326 730 135
Fragments arranged by size:
730 326 135
Bsp1286I G_dGCh'C
Cuts at: 0 326 402 661 697 1056 1191
Size: 326 76 259 36 359 135
Fragments arranged by size:
359 326 259 135 76 36
BspEI T'CCGG_A
Cuts at: 0 76 836 1153 1191
Size: 76 760 317 38
Fragments arranged by size:
760 317 76 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 337 487 924 1191
Size: 337 150 437 267
Fragments arranged by size:
437 337 267 150
BsrBI CCG'CTC
Cuts at: 0 186 623 1191
Size: 186 437 568
Fragments arranged by size:
568 437 186
BsrFI r'CCGG_y
Cuts at: 0 214 1191
Size: 214 977
BssHII G'CGCG_C
Cuts at: 0 441 1191
Size: 441 750
BssSI C'ACGA_G
Cuts at: 0 33 953 1191
Size: 33 920 238
Fragments arranged by size:
920 238 33
BstAPI GCAn_nnn'nTGC
Cuts at: 0 356 1191
Size: 356 835
BstYI r'GATC_y
Cuts at: 0 71 118 230 1191
Size: 71 47 112 961
Fragments arranged by size:
961 112 71 47
BtgI C'CryG_G
Cuts at: 0 506 1191
Size: 506 685
BtsI GCAGTG_nn'
Cuts at: 0 1070 1191
Size: 1070 121
EaeI y'GGCC_r
Cuts at: 0 140 162 614 1191
Size: 140 22 452 577
Fragments arranged by size:
577 452 140 22
EagI C'GGCC_G
Cuts at: 0 162 1191
Size: 162 1029
EarI CTCTTCn'nnn_
Cuts at: 0 685 1191
Size: 685 506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 34 411 1191
Size: 34 377 780
Fragments arranged by size:
780 377 34
EcoICRI GAG'CTC
Cuts at: 0 324 1054 1191
Size: 324 730 137
Fragments arranged by size:
730 324 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1191
Size: 307 884
EcoO109I rG'GnC_Cy
Cuts at: 0 885 1191
Size: 885 306
HaeII r_GCGC'y
Cuts at: 0 152 703 1191
Size: 152 551 488
Fragments arranged by size:
551 488 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 71 103 135 440 472 698 730 1003
Size: 71 32 32 305 32 226 32 273
Cuts at: 1003 1035 1191
Size: 32 156
Fragments arranged by size:
305 273 226 156 71 32 32 32
32 32
KasI G'GCGC_C
Cuts at: 0 148 1191
Size: 148 1043
MscI TGG'CCA
Cuts at: 0 142 1191
Size: 142 1049
MslI CAynn'nnrTG
Cuts at: 0 1114 1191
Size: 1114 77
MspA1I CmG'CkG
Cuts at: 0 404 594 1049 1191
Size: 404 190 455 142
Fragments arranged by size:
455 404 190 142
NaeI GCC'GGC
Cuts at: 0 216 1191
Size: 216 975
NarI GG'CG_CC
Cuts at: 0 149 1191
Size: 149 1042
NgoMIV G'CCGG_C
Cuts at: 0 214 1191
Size: 214 977
NspI r_CATG'y
Cuts at: 0 180 810 1191
Size: 180 630 381
Fragments arranged by size:
630 381 180
PpuMI rG'GwC_Cy
Cuts at: 0 885 1191
Size: 885 306
PspXI vC'TCGA_Gb
Cuts at: 0 313 1191
Size: 313 878
PvuII CAG'CTG
Cuts at: 0 594 1191
Size: 594 597
SacI G_AGCT'C
Cuts at: 0 326 1056 1191
Size: 326 730 135
Fragments arranged by size:
730 326 135
SapI GCTCTTCn'nnn_
Cuts at: 0 685 1191
Size: 685 506
SfcI C'TryA_G
Cuts at: 0 372 1112 1191
Size: 372 740 79
Fragments arranged by size:
740 372 79
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 139 1191
Size: 139 1052
SfoI GGC'GCC
Cuts at: 0 150 1191
Size: 150 1041
SmlI C'TyrA_G
Cuts at: 0 313 889 1191
Size: 313 576 302
Fragments arranged by size:
576 313 302
SphI G_CATG'C
Cuts at: 0 180 810 1191
Size: 180 630 381
Fragments arranged by size:
630 381 180
StyI C'CwwG_G
Cuts at: 0 90 338 411 881 1191
Size: 90 248 73 470 310
Fragments arranged by size:
470 310 248 90 73
TatI w'GTAC_w
Cuts at: 0 542 1191
Size: 542 649
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 58 90 253 285 1008 1040 1191
Size: 58 32 163 32 723 32 151
Fragments arranged by size:
723 163 151 58 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 454 715 1191
Size: 454 261 476
Fragments arranged by size:
476 454 261
XhoI C'TCGA_G
Cuts at: 0 313 1191
Size: 313 878
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AleI AlfI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BciVI BglI
BglII BlpI Bme1580I BmgBI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstYI
BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I HaeII Hin4I KasI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PpuMI PspXI PvuII SacI SapI
SfcI SfiI SfoI SmlI SphI StyI TatI TstI
Tth111I XhoI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AloI AlwNI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BclI BfrBI BmrI BmtI BplI BpmI BsaAI
BsaBI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI TaqII XbaI XcmI XmaI
XmnI ZraI