Restriction map for
pFN21AB7922
(Linear) (Six-base) MAPSORT of: KIBB7922.seq Check: 4550 from: 1 to: 1041
KIBB7922.orf
With 182 enzymes: SgfI *
March 22, 2012 15:42 ..
AccI GT'mk_AC
Cuts at: 0 357 776 1041
Size: 357 419 265
Fragments arranged by size:
419 357 265
AlwNI CAG_nnn'CTG
Cuts at: 0 385 1041
Size: 385 656
ApoI r'AATT_y
Cuts at: 0 308 671 768 1041
Size: 308 363 97 273
Fragments arranged by size:
363 308 273 97
AseI AT'TA_AT
Cuts at: 0 464 1041
Size: 464 577
AvaI C'yCGr_G
Cuts at: 0 596 1041
Size: 596 445
BanII G_rGCy'C
Cuts at: 0 597 824 1041
Size: 597 227 217
Fragments arranged by size:
597 227 217
BclI T'GATC_A
Cuts at: 0 9 828 1041
Size: 9 819 213
Fragments arranged by size:
819 213 9
Bpu10I CC'TnA_GC
Cuts at: 0 761 818 1041
Size: 761 57 223
Fragments arranged by size:
761 223 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 924 954 1041
Size: 924 30 87
Fragments arranged by size:
924 87 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 236 1041
Size: 236 805
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 1041
Size: 97 944
BsiHKAI G_wGCw'C
Cuts at: 0 735 824 1041
Size: 735 89 217
Fragments arranged by size:
735 217 89
Bsp1286I G_dGCh'C
Cuts at: 0 597 735 824 1041
Size: 597 138 89 217
Fragments arranged by size:
597 217 138 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 57 1041
Size: 57 984
BstYI r'GATC_y
Cuts at: 0 954 1041
Size: 954 87
BtsI GCAGTG_nn'
Cuts at: 0 59 1041
Size: 59 982
DraI TTT'AAA
Cuts at: 0 225 1041
Size: 225 816
EcoICRI GAG'CTC
Cuts at: 0 822 1041
Size: 822 219
EcoRI G'AATT_C
Cuts at: 0 768 1041
Size: 768 273
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 359 1041
Size: 359 682
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 9 26 41 786 818 841 873 922
Size: 9 17 15 745 32 23 32 49
Cuts at: 922 954 1041
Size: 32 87
Fragments arranged by size:
745 87 49 32 32 32 23 17
15 9
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 1041
Size: 52 989
NdeI CA'TA_TG
Cuts at: 0 179 1041
Size: 179 862
NspI r_CATG'y
Cuts at: 0 85 921 1041
Size: 85 836 120
Fragments arranged by size:
836 120 85
PflMI CCAn_nnn'nTGG
Cuts at: 0 64 1041
Size: 64 977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 942 974 1041
Size: 942 32 67
Fragments arranged by size:
942 67 32
PstI C_TGCA'G
Cuts at: 0 54 984 1041
Size: 54 930 57
Fragments arranged by size:
930 57 54
SacI G_AGCT'C
Cuts at: 0 824 1041
Size: 824 217
SfcI C'TryA_G
Cuts at: 0 50 836 980 1041
Size: 50 786 144 61
Fragments arranged by size:
786 144 61 50
SphI G_CATG'C
Cuts at: 0 85 921 1041
Size: 85 836 120
Fragments arranged by size:
836 120 85
SspI AAT'ATT
Cuts at: 0 462 1041
Size: 462 579
TatI w'GTAC_w
Cuts at: 0 560 1041
Size: 560 481
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 480 512 924 956 1041
Size: 480 32 412 32 85
Fragments arranged by size:
480 412 85 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 798 1041
Size: 798 243
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 35 1041
Size: 35 1006
Enzymes that do cut:
AccI AlwNI ApoI AseI AvaI BanII BclI Bpu10I
BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI BstYI BtsI
DraI EcoICRI EcoRI FalI Hin4I MmeI NdeI NspI
PflMI PpiI PstI SacI SfcI SphI SspI TatI
TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
StuI StyI SwaI TaqII XbaI XhoI XmaI XmnI
ZraI