Restriction map for
pFN21AB7891
(Linear) (Six-base) MAPSORT of: KIBB7891.seq Check: 238 from: 1 to: 1764
KIBB7891.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
Acc65I G'GTAC_C
Cuts at: 0 362 1764
Size: 362 1402
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1129 1764
Size: 1129 635
AhdI GACnn_n'nnGTC
Cuts at: 0 501 1764
Size: 501 1263
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 460 1764
Size: 460 1304
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 319 351 1764
Size: 319 32 1413
Fragments arranged by size:
1413 319 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1374 1764
Size: 1374 390
ApaI G_GGCC'C
Cuts at: 0 1539 1764
Size: 1539 225
ApaLI G'TGCA_C
Cuts at: 0 1101 1764
Size: 1101 663
AvaI C'yCGr_G
Cuts at: 0 94 1163 1244 1381 1532 1764
Size: 94 1069 81 137 151 232
Fragments arranged by size:
1069 232 151 137 94 81
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 337 370 1764
Size: 337 33 1394
Fragments arranged by size:
1394 337 33
BamHI G'GATC_C
Cuts at: 0 911 1764
Size: 911 853
BanI G'GyrC_C
Cuts at: 0 257 362 803 957 1333 1764
Size: 257 105 441 154 376 431
Fragments arranged by size:
441 431 376 257 154 105
BanII G_rGCy'C
Cuts at: 0 976 1539 1764
Size: 976 563 225
Fragments arranged by size:
976 563 225
BbeI G_GCGC'C
Cuts at: 0 261 1764
Size: 261 1503
BfrBI ATG'CAT
Cuts at: 0 564 1764
Size: 564 1200
BglI GCCn_nnn'nGGC
Cuts at: 0 73 1543 1708 1764
Size: 73 1470 165 56
Fragments arranged by size:
1470 165 73 56
Bme1580I G_kGCm'C
Cuts at: 0 1105 1338 1539 1764
Size: 1105 233 201 225
Fragments arranged by size:
1105 233 225 201
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 338 1764
Size: 338 1426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 462 497 837 1764
Size: 45 417 35 340 927
Fragments arranged by size:
927 417 340 45 35
Bpu10I CC'TnA_GC
Cuts at: 0 388 970 1574 1764
Size: 388 582 604 190
Fragments arranged by size:
604 582 388 190
BsaI GGTCTCn'nnnn_
Cuts at: 0 855 1764
Size: 855 909
BsaAI yAC'GTr
Cuts at: 0 1035 1764
Size: 1035 729
BsaHI Gr'CG_yC
Cuts at: 0 258 1764
Size: 258 1506
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 589 619 1764
Size: 589 30 1145
Fragments arranged by size:
1145 589 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 443 454 857 1030 1398 1578 1581
Size: 154 289 11 403 173 368 180 3
Cuts at: 1581 1764
Size: 183
Fragments arranged by size:
403 368 289 183 180 173 154 11
3
BseYI C'CCAG_C
Cuts at: 0 5 964 1499 1538 1730 1764
Size: 5 959 535 39 192 34
Fragments arranged by size:
959 535 192 39 34 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1139 1764
Size: 1139 625
BsiEI CG_ry'CG
Cuts at: 0 77 1764
Size: 77 1687
BsiHKAI G_wGCw'C
Cuts at: 0 1105 1764
Size: 1105 659
BsmI GAATG_Cn'
Cuts at: 0 1056 1764
Size: 1056 708
BsmBI CGTCTCn'nnnn_
Cuts at: 0 936 1764
Size: 936 828
Bsp1286I G_dGCh'C
Cuts at: 0 976 1105 1338 1539 1764
Size: 976 129 233 201 225
Fragments arranged by size:
976 233 225 201 129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 452 1382 1764
Size: 452 930 382
Fragments arranged by size:
930 452 382
BsrBI CCG'CTC
Cuts at: 0 1206 1764
Size: 1206 558
BsrDI GCAATG_nn'
Cuts at: 0 600 1764
Size: 600 1164
BsrFI r'CCGG_y
Cuts at: 0 76 954 1764
Size: 76 878 810
Fragments arranged by size:
878 810 76
BssHII G'CGCG_C
Cuts at: 0 433 1764
Size: 433 1331
BssSI C'ACGA_G
Cuts at: 0 498 1764
Size: 498 1266
BstAPI GCAn_nnn'nTGC
Cuts at: 0 561 1333 1764
Size: 561 772 431
Fragments arranged by size:
772 561 431
BstEII G'GTnAC_C
Cuts at: 0 503 1746 1764
Size: 503 1243 18
Fragments arranged by size:
1243 503 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1092 1138 1764
Size: 1092 46 626
Fragments arranged by size:
1092 626 46
BstYI r'GATC_y
Cuts at: 0 911 1133 1764
Size: 911 222 631
Fragments arranged by size:
911 631 222
Bsu36I CC'TnA_GG
Cuts at: 0 323 906 1764
Size: 323 583 858
Fragments arranged by size:
858 583 323
BtgI C'CryG_G
Cuts at: 0 292 1413 1764
Size: 292 1121 351
Fragments arranged by size:
1121 351 292
BtsI GCAGTG_nn'
Cuts at: 0 986 1764
Size: 986 778
ClaI AT'CG_AT
Cuts at: 0 644 1764
Size: 644 1120
DraI TTT'AAA
Cuts at: 0 342 1764
Size: 342 1422
DraIII CAC_nnn'GTG
Cuts at: 0 876 1764
Size: 876 888
EaeI y'GGCC_r
Cuts at: 0 74 1436 1764
Size: 74 1362 328
Fragments arranged by size:
1362 328 74
EagI C'GGCC_G
Cuts at: 0 74 1764
Size: 74 1690
EarI CTCTTCn'nnn_
Cuts at: 0 1264 1270 1764
Size: 1264 6 494
Fragments arranged by size:
1264 494 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 95 265 1764
Size: 95 170 1499
Fragments arranged by size:
1499 170 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 462 497 837 1129 1764
Size: 45 417 35 340 292 635
Fragments arranged by size:
635 417 340 292 45 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 187 1764
Size: 187 1577
EcoO109I rG'GnC_Cy
Cuts at: 0 180 346 1710 1764
Size: 180 166 1364 54
Fragments arranged by size:
1364 180 166 54
HaeII r_GCGC'y
Cuts at: 0 68 167 261 485 1764
Size: 68 99 94 224 1279
Fragments arranged by size:
1279 224 99 94 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 151 181 183 213 460 487 492 519
Size: 151 30 2 30 247 27 5 27
Cuts at: 519 589 621 1764
Size: 70 32 1143
Fragments arranged by size:
1143 247 151 70 32 30 30 27
27 5 2
HincII GTy'rAC
Cuts at: 0 210 1764
Size: 210 1554
KasI G'GCGC_C
Cuts at: 0 257 1764
Size: 257 1507
KpnI G_GTAC'C
Cuts at: 0 366 1764
Size: 366 1398
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 431 702 1764
Size: 197 234 271 1062
Fragments arranged by size:
1062 271 234 197
MspA1I CmG'CkG
Cuts at: 0 44 177 294 570 1290 1468 1542 1764
Size: 44 133 117 276 720 178 74 222
Fragments arranged by size:
720 276 222 178 133 117 74 44
NaeI GCC'GGC
Cuts at: 0 78 1764
Size: 78 1686
NarI GG'CG_CC
Cuts at: 0 258 1764
Size: 258 1506
NgoMIV G'CCGG_C
Cuts at: 0 76 1764
Size: 76 1688
NsiI A_TGCA'T
Cuts at: 0 566 1764
Size: 566 1198
NspI r_CATG'y
Cuts at: 0 564 1425 1764
Size: 564 861 339
Fragments arranged by size:
861 564 339
PasI CC'CwG_GG
Cuts at: 0 358 1764
Size: 358 1406
PfoI T'CCnGG_A
Cuts at: 0 122 272 380 423 711 1319 1764
Size: 122 150 108 43 288 608 445
Fragments arranged by size:
608 445 288 150 122 108 43
PmlI CAC'GTG
Cuts at: 0 1035 1764
Size: 1035 729
PpuMI rG'GwC_Cy
Cuts at: 0 180 346 1764
Size: 180 166 1418
Fragments arranged by size:
1418 180 166
PshAI GACnn'nnGTC
Cuts at: 0 417 1764
Size: 417 1347
PspOMI G'GGCC_C
Cuts at: 0 1535 1764
Size: 1535 229
PstI C_TGCA'G
Cuts at: 0 449 981 1452 1584 1764
Size: 449 532 471 132 180
Fragments arranged by size:
532 471 449 180 132
PvuII CAG'CTG
Cuts at: 0 1290 1468 1542 1764
Size: 1290 178 74 222
Fragments arranged by size:
1290 222 178 74
SacII CC_GC'GG
Cuts at: 0 295 1764
Size: 295 1469
ScaI AGT'ACT
Cuts at: 0 1183 1764
Size: 1183 581
SexAI A'CCwGG_T
Cuts at: 0 1234 1764
Size: 1234 530
SfcI C'TryA_G
Cuts at: 0 445 588 977 1448 1580 1723 1764
Size: 445 143 389 471 132 143 41
Fragments arranged by size:
471 445 389 143 143 132 41
SfoI GGC'GCC
Cuts at: 0 259 1764
Size: 259 1505
SmaI CCC'GGG
Cuts at: 0 1246 1534 1764
Size: 1246 288 230
Fragments arranged by size:
1246 288 230
SmlI C'TyrA_G
Cuts at: 0 1163 1764
Size: 1163 601
SphI G_CATG'C
Cuts at: 0 1425 1764
Size: 1425 339
StuI AGG'CCT
Cuts at: 0 321 1764
Size: 321 1443
StyI C'CwwG_G
Cuts at: 0 1085 1137 1641 1764
Size: 1085 52 504 123
Fragments arranged by size:
1085 504 123 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 640 692 1498 1764
Size: 640 52 806 266
Fragments arranged by size:
806 640 266 52
TatI w'GTAC_w
Cuts at: 0 776 1181 1764
Size: 776 405 583
Fragments arranged by size:
776 583 405
Tth111I GACn'n_nGTC
Cuts at: 0 190 1302 1764
Size: 190 1112 462
Fragments arranged by size:
1112 462 190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1662 1764
Size: 1662 102
XhoI C'TCGA_G
Cuts at: 0 1163 1764
Size: 1163 601
XmaI C'CCGG_G
Cuts at: 0 1244 1532 1764
Size: 1244 288 232
Fragments arranged by size:
1244 288 232
XmnI GAAnn'nnTTC
Cuts at: 0 1054 1764
Size: 1054 710
Enzymes that do cut:
Acc65I AcuI AhdI AlfI AloI AlwNI ApaI ApaLI
AvaI BaeI BamHI BanI BanII BbeI BfrBI BglI
Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI ClaI DraI DraIII
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII KasI KpnI MmeI MspA1I NaeI NarI
NgoMIV NsiI NspI PasI PfoI PmlI PpuMI PshAI
PspOMI PstI PvuII SacII ScaI SexAI SfcI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI Tth111I
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI ApoI AscI AseI AsiSI AvrII BbsI BbvCI
BcgI BciVI BclI BglII BlpI BmgBI BmrI BmtI
BpuEI BsaBI BsaWI BsiWI BspEI BspHI BsrGI BstBI
BstZ17I BtgZI CspCI DrdI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI MfeI MluI MscI
MslI NcoI NdeI NheI NotI NruI PacI PciI
PflMI PmeI PpiI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TstI XbaI ZraI