Restriction map for
pFN21AB7881
(Linear) (Six-base) MAPSORT of: KIBB7881.seq Check: 1111 from: 1 to: 1734
KIBB7881.orf
With 182 enzymes: SgfI *
July 3, 2012 16:30 ..
AccI GT'mk_AC
Cuts at: 0 565 1734
Size: 565 1169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 26 74 144 1059 1143 1395 1479
Size: 8 18 48 70 915 84 252 84
Cuts at: 1479 1734
Size: 255
Fragments arranged by size:
915 255 252 84 84 70 48 18
8
AflIII A'CryG_T
Cuts at: 0 761 1734
Size: 761 973
AhdI GACnn_n'nnGTC
Cuts at: 0 415 1734
Size: 415 1319
AleI CACnn'nnGTG
Cuts at: 0 1706 1734
Size: 1706 28
AlwNI CAG_nnn'CTG
Cuts at: 0 237 270 327 475 1734
Size: 237 33 57 148 1259
Fragments arranged by size:
1259 237 148 57 33
ApaI G_GGCC'C
Cuts at: 0 222 1734
Size: 222 1512
ApoI r'AATT_y
Cuts at: 0 750 1613 1734
Size: 750 863 121
Fragments arranged by size:
863 750 121
AvaI C'yCGr_G
Cuts at: 0 194 905 1734
Size: 194 711 829
Fragments arranged by size:
829 711 194
BanII G_rGCy'C
Cuts at: 0 222 320 1003 1423 1734
Size: 222 98 683 420 311
Fragments arranged by size:
683 420 311 222 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 674 1734
Size: 674 1060
BbvCI CC'TCA_GC
Cuts at: 0 238 1734
Size: 238 1496
Bme1580I G_kGCm'C
Cuts at: 0 222 539 1734
Size: 222 317 1195
Fragments arranged by size:
1195 317 222
BmrI ACTGGGnnnn_n'
Cuts at: 0 1043 1211 1379 1463 1734
Size: 1043 168 168 84 271
Fragments arranged by size:
1043 271 168 168 84
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 599 1734
Size: 599 1135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 304 307 515 626 1041 1559 1734
Size: 292 12 3 208 111 415 518 175
Fragments arranged by size:
518 415 292 208 175 111 12 3
Bpu10I CC'TnA_GC
Cuts at: 0 238 1002 1734
Size: 238 764 732
Fragments arranged by size:
764 732 238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1734
Size: 910 824
BsaI GGTCTCn'nnnn_
Cuts at: 0 602 775 902 1287 1600 1734
Size: 602 173 127 385 313 134
Fragments arranged by size:
602 385 313 173 134 127
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 347 377 751 781 1734
Size: 347 30 374 30 953
Fragments arranged by size:
953 374 347 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 328 373 469 709 1458 1734
Size: 97 231 45 96 240 749 276
Fragments arranged by size:
749 276 240 231 97 96 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 1168 1252 1734
Size: 461 707 84 482
Fragments arranged by size:
707 482 461 84
BsiHKAI G_wGCw'C
Cuts at: 0 320 1003 1423 1734
Size: 320 683 420 311
Fragments arranged by size:
683 420 320 311
BsmBI CGTCTCn'nnnn_
Cuts at: 0 951 1734
Size: 951 783
Bsp1286I G_dGCh'C
Cuts at: 0 222 320 539 1003 1423 1734
Size: 222 98 219 464 420 311
Fragments arranged by size:
464 420 311 222 219 98
BsrDI GCAATG_nn'
Cuts at: 0 1482 1734
Size: 1482 252
BstEII G'GTnAC_C
Cuts at: 0 366 1734
Size: 366 1368
BstXI CCAn_nnnn'nTGG
Cuts at: 0 844 1734
Size: 844 890
BstYI r'GATC_y
Cuts at: 0 60 266 723 1734
Size: 60 206 457 1011
Fragments arranged by size:
1011 457 206 60
Bsu36I CC'TnA_GG
Cuts at: 0 311 1734
Size: 311 1423
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1212 1247 1464 1499 1734
Size: 1212 35 217 35 235
Fragments arranged by size:
1212 235 217 35 35
EaeI y'GGCC_r
Cuts at: 0 135 1734
Size: 135 1599
EarI CTCTTCn'nnn_
Cuts at: 0 50 1734
Size: 50 1684
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 139 1431 1734
Size: 139 1292 303
Fragments arranged by size:
1292 303 139
EcoICRI GAG'CTC
Cuts at: 0 318 1001 1421 1734
Size: 318 683 420 313
Fragments arranged by size:
683 420 318 313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 26 74 144 292 304 307 515
Size: 8 18 48 70 148 12 3 208
Cuts at: 515 626 1041 1059 1143 1395 1479 1559 1734
Size: 111 415 18 84 252 84 80 175
Fragments arranged by size:
415 252 208 175 148 111 84 84
80 70 48 18 18 12 8 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 310 376 1734
Size: 310 66 1358
Fragments arranged by size:
1358 310 66
EcoO109I rG'GnC_Cy
Cuts at: 0 190 219 1490 1734
Size: 190 29 1271 244
Fragments arranged by size:
1271 244 190 29
EcoRI G'AATT_C
Cuts at: 0 1613 1734
Size: 1613 121
EcoRV GAT'ATC
Cuts at: 0 1110 1734
Size: 1110 624
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 38 79 1734
Size: 38 41 1655
Fragments arranged by size:
1655 41 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 401 433 1410 1442 1662 1694
Size: 91 32 278 32 977 32 220 32
Cuts at: 1694 1734
Size: 40
Fragments arranged by size:
977 278 220 91 40 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 987 1322 1574 1734
Size: 987 335 252 160
Fragments arranged by size:
987 335 252 160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 1734
Size: 234 1500
MscI TGG'CCA
Cuts at: 0 137 1734
Size: 137 1597
MslI CAynn'nnrTG
Cuts at: 0 676 968 1218 1706 1734
Size: 676 292 250 488 28
Fragments arranged by size:
676 488 292 250 28
MspA1I CmG'CkG
Cuts at: 0 856 936 1734
Size: 856 80 798
Fragments arranged by size:
856 798 80
NspI r_CATG'y
Cuts at: 0 765 1734
Size: 765 969
PasI CC'CwG_GG
Cuts at: 0 771 1734
Size: 771 963
PciI A'CATG_T
Cuts at: 0 761 1734
Size: 761 973
PflMI CCAn_nnn'nTGG
Cuts at: 0 327 654 1734
Size: 327 327 1080
Fragments arranged by size:
1080 327 327
PfoI T'CCnGG_A
Cuts at: 0 269 1734
Size: 269 1465
PpuMI rG'GwC_Cy
Cuts at: 0 190 1734
Size: 190 1544
PspOMI G'GGCC_C
Cuts at: 0 218 1734
Size: 218 1516
PvuII CAG'CTG
Cuts at: 0 856 1734
Size: 856 878
SacI G_AGCT'C
Cuts at: 0 320 1003 1423 1734
Size: 320 683 420 311
Fragments arranged by size:
683 420 320 311
SmlI C'TyrA_G
Cuts at: 0 889 1734
Size: 889 845
StuI AGG'CCT
Cuts at: 0 1007 1175 1511 1734
Size: 1007 168 336 223
Fragments arranged by size:
1007 336 223 168
StyI C'CwwG_G
Cuts at: 0 257 1734
Size: 257 1477
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 763 1734
Size: 763 971
TatI w'GTAC_w
Cuts at: 0 462 1734
Size: 462 1272
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 347 379 1734
Size: 347 32 1355
Fragments arranged by size:
1355 347 32
XmnI GAAnn'nnTTC
Cuts at: 0 21 1240 1324 1408 1576 1734
Size: 21 1219 84 84 168 158
Fragments arranged by size:
1219 168 158 84 84 21
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlwNI ApaI ApoI
AvaI BanII BbsI BbvCI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaXI BseRI BsgI BsiHKAI BsmBI
Bsp1286I BsrDI BstEII BstXI BstYI Bsu36I CspCI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI Hin4I HindIII MmeI MscI MslI MspA1I NspI
PasI PciI PflMI PfoI PpuMI PspOMI PvuII SacI
SmlI StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EagI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XcmI XhoI XmaI ZraI