Restriction map for
pFN21AB7806
(Linear) (Six-base) MAPSORT of: KIBB7806.seq Check: 4958 from: 1 to: 1779
KIBB7806.orf
With 182 enzymes: SgfI *
March 22, 2012 15:41 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 727 759 1779
Size: 727 32 1020
Fragments arranged by size:
1020 727 32
AlwNI CAG_nnn'CTG
Cuts at: 0 60 78 490 1228 1285 1779
Size: 60 18 412 738 57 494
Fragments arranged by size:
738 494 412 60 57 18
ApaI G_GGCC'C
Cuts at: 0 117 254 481 1779
Size: 117 137 227 1298
Fragments arranged by size:
1298 227 137 117
ApaLI G'TGCA_C
Cuts at: 0 1427 1779
Size: 1427 352
ApoI r'AATT_y
Cuts at: 0 727 1779
Size: 727 1052
AscI GG'CGCG_CC
Cuts at: 0 49 1779
Size: 49 1730
AvaI C'yCGr_G
Cuts at: 0 116 145 281 304 406 1779
Size: 116 29 136 23 102 1373
Fragments arranged by size:
1373 136 116 102 29 23
BanI G'GyrC_C
Cuts at: 0 502 1049 1534 1779
Size: 502 547 485 245
Fragments arranged by size:
547 502 485 245
BanII G_rGCy'C
Cuts at: 0 117 146 171 254 301 481 1013 1779
Size: 117 29 25 83 47 180 532 766
Fragments arranged by size:
766 532 180 117 83 47 29 25
BbeI G_GCGC'C
Cuts at: 0 1053 1779
Size: 1053 726
BbsI GAAGACnn'nnnn_
Cuts at: 0 407 1779
Size: 407 1372
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 76 110 1779
Size: 76 34 1669
Fragments arranged by size:
1669 76 34
BglI GCCn_nnn'nGGC
Cuts at: 0 184 196 236 485 1396 1456 1734 1779
Size: 184 12 40 249 911 60 278 45
Fragments arranged by size:
911 278 249 184 60 45 40 12
BlpI GC'TnA_GC
Cuts at: 0 1607 1779
Size: 1607 172
Bme1580I G_kGCm'C
Cuts at: 0 117 156 254 481 505 1098 1431 1537
Size: 117 39 98 227 24 593 333 106
Cuts at: 1537 1779
Size: 242
Fragments arranged by size:
593 333 242 227 117 106 98 39
24
BmgBI CAC'GTC
Cuts at: 0 914 1779
Size: 914 865
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1221 1779
Size: 1221 558
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 997 1378 1417 1779
Size: 82 915 381 39 362
Fragments arranged by size:
915 381 362 82 39
BsaAI yAC'GTr
Cuts at: 0 630 828 1779
Size: 630 198 951
Fragments arranged by size:
951 630 198
BsaHI Gr'CG_yC
Cuts at: 0 1050 1779
Size: 1050 729
BsaWI w'CCGG_w
Cuts at: 0 1374 1702 1779
Size: 1374 328 77
Fragments arranged by size:
1374 328 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 13 43 337 367 1150 1180 1415 1445
Size: 13 30 294 30 783 30 235 30
Cuts at: 1445 1620 1650 1779
Size: 175 30 129
Fragments arranged by size:
783 294 235 175 129 30 30 30
30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 283 346 397 400 403 433 436
Size: 280 3 63 51 3 3 30 3
Cuts at: 436 439 442 445 463 466 469 472 481
Size: 3 3 3 18 3 3 3 9
Cuts at: 481 691 1101 1213 1779
Size: 210 410 112 566
Fragments arranged by size:
566 410 280 210 112 63 51 30
18 9 3 3 3 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 222 474 530 573 1460 1723 1779
Size: 222 252 56 43 887 263 56
Fragments arranged by size:
887 263 252 222 56 56 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 947 1304 1779
Size: 83 864 357 475
Fragments arranged by size:
864 475 357 83
BsiEI CG_ry'CG
Cuts at: 0 111 592 1689 1779
Size: 111 481 1097 90
Fragments arranged by size:
1097 481 111 90
BsiHKAI G_wGCw'C
Cuts at: 0 338 1013 1431 1779
Size: 338 675 418 348
Fragments arranged by size:
675 418 348 338
BsmI GAATG_Cn'
Cuts at: 0 1475 1779
Size: 1475 304
BsmBI CGTCTCn'nnnn_
Cuts at: 0 16 1779
Size: 16 1763
Bsp1286I G_dGCh'C
Cuts at: 0 117 146 156 171 254 301 338 481
Size: 117 29 10 15 83 47 37 143
Cuts at: 481 505 1013 1098 1431 1537 1779
Size: 24 508 85 333 106 242
Fragments arranged by size:
508 333 242 143 117 106 85 83
47 37 29 24 15 10
BspEI T'CCGG_A
Cuts at: 0 1374 1779
Size: 1374 405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 914 1779
Size: 914 865
BsrBI CCG'CTC
Cuts at: 0 588 1779
Size: 588 1191
BsrFI r'CCGG_y
Cuts at: 0 1046 1614 1779
Size: 1046 568 165
Fragments arranged by size:
1046 568 165
BsrGI T'GTAC_A
Cuts at: 0 1586 1779
Size: 1586 193
BssHII G'CGCG_C
Cuts at: 0 49 1779
Size: 49 1730
BssSI C'ACGA_G
Cuts at: 0 853 1779
Size: 853 926
BstAPI GCAn_nnn'nTGC
Cuts at: 0 78 1779
Size: 78 1701
BstXI CCAn_nnnn'nTGG
Cuts at: 0 573 1060 1779
Size: 573 487 719
Fragments arranged by size:
719 573 487
BtgI C'CryG_G
Cuts at: 0 506 549 657 1451 1779
Size: 506 43 108 794 328
Fragments arranged by size:
794 506 328 108 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 569 1779
Size: 569 1210
DraIII CAC_nnn'GTG
Cuts at: 0 1263 1779
Size: 1263 516
EaeI y'GGCC_r
Cuts at: 0 108 176 589 794 1145 1230 1397 1448
Size: 108 68 413 205 351 85 167 51
Cuts at: 1448 1686 1716 1779
Size: 238 30 63
Fragments arranged by size:
413 351 238 205 167 108 85 68
63 51 30
EagI C'GGCC_G
Cuts at: 0 108 589 1686 1779
Size: 108 481 1097 93
Fragments arranged by size:
1097 481 108 93
EcoICRI GAG'CTC
Cuts at: 0 1011 1779
Size: 1011 768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 997 1378 1417 1779
Size: 82 915 381 39 362
Fragments arranged by size:
915 381 362 82 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 409 856 1779
Size: 409 447 923
Fragments arranged by size:
923 447 409
EcoO109I rG'GnC_Cy
Cuts at: 0 251 478 487 595 711 804 897 990
Size: 251 227 9 108 116 93 93 93
Cuts at: 990 1779
Size: 789
Fragments arranged by size:
789 251 227 116 108 93 93 93
9
EcoRI G'AATT_C
Cuts at: 0 727 1779
Size: 727 1052
HaeII r_GCGC'y
Cuts at: 0 1053 1779
Size: 1053 726
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 337 369 1779
Size: 337 32 1410
Fragments arranged by size:
1410 337 32
KasI G'GCGC_C
Cuts at: 0 1049 1779
Size: 1049 730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1779
Size: 380 1399
MscI TGG'CCA
Cuts at: 0 796 1450 1779
Size: 796 654 329
Fragments arranged by size:
796 654 329
MslI CAynn'nnrTG
Cuts at: 0 1558 1564 1779
Size: 1558 6 215
Fragments arranged by size:
1558 215 6
MspA1I CmG'CkG
Cuts at: 0 36 45 78 165 205 551 642 1285
Size: 36 9 33 87 40 346 91 643
Cuts at: 1285 1312 1330 1344 1464 1712 1732 1779
Size: 27 18 14 120 248 20 47
Fragments arranged by size:
643 346 248 120 91 87 47 40
36 33 27 20 18 14 9
NaeI GCC'GGC
Cuts at: 0 1048 1616 1779
Size: 1048 568 163
Fragments arranged by size:
1048 568 163
NarI GG'CG_CC
Cuts at: 0 1050 1779
Size: 1050 729
NcoI C'CATG_G
Cuts at: 0 1451 1779
Size: 1451 328
NgoMIV G'CCGG_C
Cuts at: 0 1046 1614 1779
Size: 1046 568 165
Fragments arranged by size:
1046 568 165
NspI r_CATG'y
Cuts at: 0 1107 1563 1779
Size: 1107 456 216
Fragments arranged by size:
1107 456 216
PasI CC'CwG_GG
Cuts at: 0 566 1137 1779
Size: 566 571 642
Fragments arranged by size:
642 571 566
PflMI CCAn_nnn'nTGG
Cuts at: 0 60 1263 1779
Size: 60 1203 516
Fragments arranged by size:
1203 516 60
PfoI T'CCnGG_A
Cuts at: 0 731 1779
Size: 731 1048
PpuMI rG'GwC_Cy
Cuts at: 0 711 804 1779
Size: 711 93 975
Fragments arranged by size:
975 711 93
PspOMI G'GGCC_C
Cuts at: 0 113 250 477 1779
Size: 113 137 227 1302
Fragments arranged by size:
1302 227 137 113
PstI C_TGCA'G
Cuts at: 0 204 647 1368 1779
Size: 204 443 721 411
Fragments arranged by size:
721 443 411 204
PvuII CAG'CTG
Cuts at: 0 78 205 642 1285 1312 1330 1344 1464
Size: 78 127 437 643 27 18 14 120
Cuts at: 1464 1779
Size: 315
Fragments arranged by size:
643 437 315 127 120 78 27 18
14
SacI G_AGCT'C
Cuts at: 0 1013 1779
Size: 1013 766
SacII CC_GC'GG
Cuts at: 0 552 1779
Size: 552 1227
SanDI GG'GwC_CC
Cuts at: 0 711 1779
Size: 711 1068
SfcI C'TryA_G
Cuts at: 0 7 200 643 1364 1779
Size: 7 193 443 721 415
Fragments arranged by size:
721 443 415 193 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 485 1396 1456 1779
Size: 485 911 60 323
Fragments arranged by size:
911 485 323 60
SfoI GGC'GCC
Cuts at: 0 1051 1779
Size: 1051 728
SmaI CCC'GGG
Cuts at: 0 118 283 306 1779
Size: 118 165 23 1473
Fragments arranged by size:
1473 165 118 23
SphI G_CATG'C
Cuts at: 0 1107 1779
Size: 1107 672
SrfI GCCC'GGGC
Cuts at: 0 118 1779
Size: 118 1661
StuI AGG'CCT
Cuts at: 0 544 1779
Size: 544 1235
StyI C'CwwG_G
Cuts at: 0 893 1451 1779
Size: 893 558 328
Fragments arranged by size:
893 558 328
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 236 1131 1779
Size: 236 895 648
Fragments arranged by size:
895 648 236
TatI w'GTAC_w
Cuts at: 0 1586 1779
Size: 1586 193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1060 1779
Size: 1060 719
XmaI C'CCGG_G
Cuts at: 0 116 281 304 1779
Size: 116 165 23 1475
Fragments arranged by size:
1475 165 116 23
XmnI GAAnn'nnTTC
Cuts at: 0 727 1779
Size: 727 1052
Enzymes that do cut:
AloI AlwNI ApaI ApaLI ApoI AscI AvaI BanI
BanII BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI
BplI BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstXI BtgI
BtgZI DraIII EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SfcI
SfiI SfoI SmaI SphI SrfI StuI StyI TaqII
TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BclI BfrBI BglII BmrI
BmtI Bpu10I BpuEI BsaI BsaBI BsiWI BspHI BsrDI
BstBI BstEII BstYI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DrdI EarI EciI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SapI SbfI ScaI SexAI SgfI SgrAI SmlI SnaBI
SpeI SspI SwaI TstI Tth111I XbaI XhoI ZraI