Restriction map for
pFN21AB7783
(Linear) (Six-base) MAPSORT of: KIBB7783.seq Check: 683 from: 1 to: 1371
KIBB7783.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AccI GT'mk_AC
Cuts at: 0 359 1371
Size: 359 1012
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 401 1371
Size: 172 229 970
Fragments arranged by size:
970 229 172
AleI CACnn'nnGTG
Cuts at: 0 515 1193 1371
Size: 515 678 178
Fragments arranged by size:
678 515 178
AlwNI CAG_nnn'CTG
Cuts at: 0 493 693 925 1371
Size: 493 200 232 446
Fragments arranged by size:
493 446 232 200
ApoI r'AATT_y
Cuts at: 0 960 1023 1371
Size: 960 63 348
Fragments arranged by size:
960 348 63
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1197 1230 1371
Size: 1197 33 141
Fragments arranged by size:
1197 141 33
BanI G'GyrC_C
Cuts at: 0 57 103 1285 1361 1371
Size: 57 46 1182 76 10
Fragments arranged by size:
1182 76 57 46 10
BbeI G_GCGC'C
Cuts at: 0 61 107 1289 1371
Size: 61 46 1182 82
Fragments arranged by size:
1182 82 61 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 405 1151 1371
Size: 405 746 220
Fragments arranged by size:
746 405 220
BbvCI CC'TCA_GC
Cuts at: 0 293 1371
Size: 293 1078
BglII A'GATC_T
Cuts at: 0 645 1371
Size: 645 726
BlpI GC'TnA_GC
Cuts at: 0 16 1371
Size: 16 1355
Bme1580I G_kGCm'C
Cuts at: 0 47 1371
Size: 47 1324
BmrI ACTGGGnnnn_n'
Cuts at: 0 713 1371
Size: 713 658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 287 584 940 1254 1371
Size: 227 60 297 356 314 117
Fragments arranged by size:
356 314 297 227 117 60
Bpu10I CC'TnA_GC
Cuts at: 0 293 1371
Size: 293 1078
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 1371
Size: 73 1298
BsaI GGTCTCn'nnnn_
Cuts at: 0 456 1371
Size: 456 915
BsaBI GATnn'nnATC
Cuts at: 0 650 1371
Size: 650 721
BsaHI Gr'CG_yC
Cuts at: 0 58 104 1286 1371
Size: 58 46 1182 85
Fragments arranged by size:
1182 85 58 46
BsaWI w'CCGG_w
Cuts at: 0 702 1271 1371
Size: 702 569 100
Fragments arranged by size:
702 569 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 516 546 582 612 957 987 1371
Size: 516 30 36 30 345 30 384
Fragments arranged by size:
516 384 345 36 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 524 1371
Size: 160 364 847
Fragments arranged by size:
847 364 160
BseYI C'CCAG_C
Cuts at: 0 47 99 212 668 1371
Size: 47 52 113 456 703
Fragments arranged by size:
703 456 113 52 47
BsiEI CG_ry'CG
Cuts at: 0 1249 1371
Size: 1249 122
Bsp1286I G_dGCh'C
Cuts at: 0 47 1371
Size: 47 1324
BsrDI GCAATG_nn'
Cuts at: 0 749 1371
Size: 749 622
BsrFI r'CCGG_y
Cuts at: 0 54 72 93 207 226 1371
Size: 54 18 21 114 19 1145
Fragments arranged by size:
1145 114 54 21 19 18
BstEII G'GTnAC_C
Cuts at: 0 386 1371
Size: 386 985
BstYI r'GATC_y
Cuts at: 0 645 705 1371
Size: 645 60 666
Fragments arranged by size:
666 645 60
Bsu36I CC'TnA_GG
Cuts at: 0 1180 1371
Size: 1180 191
BtgI C'CryG_G
Cuts at: 0 232 776 1371
Size: 232 544 595
Fragments arranged by size:
595 544 232
BtsI GCAGTG_nn'
Cuts at: 0 827 1371
Size: 827 544
DraI TTT'AAA
Cuts at: 0 1169 1371
Size: 1169 202
EarI CTCTTCn'nnn_
Cuts at: 0 139 1351 1371
Size: 139 1212 20
Fragments arranged by size:
1212 139 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 227 287 401 584 940 1254 1371
Size: 172 55 60 114 183 356 314 117
Fragments arranged by size:
356 314 183 172 117 114 60 55
EcoO109I rG'GnC_Cy
Cuts at: 0 37 198 249 552 1371
Size: 37 161 51 303 819
Fragments arranged by size:
819 303 161 51 37
HaeII r_GCGC'y
Cuts at: 0 29 61 107 133 1289 1371
Size: 29 32 46 26 1156 82
Fragments arranged by size:
1156 82 46 32 29 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 334 366 516 548 582 614 1371
Size: 334 32 150 32 34 32 757
Fragments arranged by size:
757 334 150 34 32 32 32
HincII GTy'rAC
Cuts at: 0 1047 1371
Size: 1047 324
HpaI GTT'AAC
Cuts at: 0 1047 1371
Size: 1047 324
KasI G'GCGC_C
Cuts at: 0 57 103 1285 1371
Size: 57 46 1182 86
Fragments arranged by size:
1182 86 57 46
MfeI C'AATT_G
Cuts at: 0 992 1371
Size: 992 379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1086 1371
Size: 1086 285
MslI CAynn'nnrTG
Cuts at: 0 515 1193 1209 1371
Size: 515 678 16 162
Fragments arranged by size:
678 515 162 16
MspA1I CmG'CkG
Cuts at: 0 234 772 1371
Size: 234 538 599
Fragments arranged by size:
599 538 234
NaeI GCC'GGC
Cuts at: 0 56 209 1371
Size: 56 153 1162
Fragments arranged by size:
1162 153 56
NarI GG'CG_CC
Cuts at: 0 58 104 1286 1371
Size: 58 46 1182 85
Fragments arranged by size:
1182 85 58 46
NcoI C'CATG_G
Cuts at: 0 776 1371
Size: 776 595
NdeI CA'TA_TG
Cuts at: 0 1352 1371
Size: 1352 19
NgoMIV G'CCGG_C
Cuts at: 0 54 207 1371
Size: 54 153 1164
Fragments arranged by size:
1164 153 54
NspI r_CATG'y
Cuts at: 0 843 1371
Size: 843 528
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 1371
Size: 61 32 1278
Fragments arranged by size:
1278 61 32
PpuMI rG'GwC_Cy
Cuts at: 0 37 249 1371
Size: 37 212 1122
Fragments arranged by size:
1122 212 37
PstI C_TGCA'G
Cuts at: 0 822 1371
Size: 822 549
PvuI CG_AT'CG
Cuts at: 0 1249 1371
Size: 1249 122
PvuII CAG'CTG
Cuts at: 0 772 1371
Size: 772 599
SacII CC_GC'GG
Cuts at: 0 235 1371
Size: 235 1136
SanDI GG'GwC_CC
Cuts at: 0 37 1371
Size: 37 1334
SapI GCTCTTCn'nnn_
Cuts at: 0 139 1371
Size: 139 1232
SexAI A'CCwGG_T
Cuts at: 0 251 1371
Size: 251 1120
SfcI C'TryA_G
Cuts at: 0 818 1371
Size: 818 553
SfoI GGC'GCC
Cuts at: 0 59 105 1287 1371
Size: 59 46 1182 84
Fragments arranged by size:
1182 84 59 46
SgrAI Cr'CCGG_yG
Cuts at: 0 54 1371
Size: 54 1317
SmlI C'TyrA_G
Cuts at: 0 88 1371
Size: 88 1283
SphI G_CATG'C
Cuts at: 0 843 1371
Size: 843 528
SspI AAT'ATT
Cuts at: 0 615 1371
Size: 615 756
StuI AGG'CCT
Cuts at: 0 785 1371
Size: 785 586
StyI C'CwwG_G
Cuts at: 0 776 1371
Size: 776 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 882 914 1043 1075 1371
Size: 882 32 129 32 296
Fragments arranged by size:
882 296 129 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 253 1371
Size: 253 1118
XbaI T'CTAG_A
Cuts at: 0 708 1371
Size: 708 663
XmnI GAAnn'nnTTC
Cuts at: 0 412 829 1371
Size: 412 417 542
Fragments arranged by size:
542 417 412
Enzymes that do cut:
AccI AcuI AleI AlwNI ApoI BaeI BanI BbeI
BbsI BbvCI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI Bsp1286I BsrDI BsrFI BstEII BstYI Bsu36I BtgI
BtsI DraI EarI Eco57MI EcoO109I HaeII Hin4I HincII
HpaI KasI MfeI MmeI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PpiI PpuMI PstI PvuI
PvuII SacII SanDI SapI SexAI SfcI SfoI SgrAI
SmlI SphI SspI StuI StyI TstI Tth111I XbaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BanII BcgI BciVI BclI BfrBI
BglI BmgBI BmtI BplI BsaAI BsgI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII KpnI MluI
MscI NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI RsrII SacI SalI SbfI ScaI SfiI SgfI
SmaI SnaBI SpeI SrfI SwaI TaqII TatI XcmI
XhoI XmaI ZraI