Restriction map for
pFN21AB7768
(Linear) (Six-base) MAPSORT of: KIBB7768.seq Check: 1764 from: 1 to: 1230
KIBB7768.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 298 1230
Size: 298 932
AatII G_ACGT'C
Cuts at: 0 1160 1230
Size: 1160 70
Acc65I G'GTAC_C
Cuts at: 0 446 1230
Size: 446 784
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 119 290 313 1230
Size: 65 54 171 23 917
Fragments arranged by size:
917 171 65 54 23
AflIII A'CryG_T
Cuts at: 0 455 1230
Size: 455 775
AleI CACnn'nnGTG
Cuts at: 0 1115 1230
Size: 1115 115
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 520 552 1230
Size: 520 32 678
Fragments arranged by size:
678 520 32
AlwNI CAG_nnn'CTG
Cuts at: 0 57 147 518 1230
Size: 57 90 371 712
Fragments arranged by size:
712 371 90 57
ApaI G_GGCC'C
Cuts at: 0 24 119 1029 1230
Size: 24 95 910 201
Fragments arranged by size:
910 201 95 24
AvaI C'yCGr_G
Cuts at: 0 523 1230
Size: 523 707
BanI G'GyrC_C
Cuts at: 0 446 1230
Size: 446 784
BanII G_rGCy'C
Cuts at: 0 24 119 629 755 1029 1230
Size: 24 95 510 126 274 201
Fragments arranged by size:
510 274 201 126 95 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 53 1230
Size: 53 1177
BbvCI CC'TCA_GC
Cuts at: 0 58 1230
Size: 58 1172
BlpI GC'TnA_GC
Cuts at: 0 623 1230
Size: 623 607
Bme1580I G_kGCm'C
Cuts at: 0 24 119 1029 1191 1230
Size: 24 95 910 162 39
Fragments arranged by size:
910 162 95 39 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 769 882 1230
Size: 586 183 113 348
Fragments arranged by size:
586 348 183 113
Bpu10I CC'TnA_GC
Cuts at: 0 58 652 697 1230
Size: 58 594 45 533
Fragments arranged by size:
594 533 58 45
BsaI GGTCTCn'nnnn_
Cuts at: 0 947 1128 1230
Size: 947 181 102
Fragments arranged by size:
947 181 102
BsaAI yAC'GTr
Cuts at: 0 354 1230
Size: 354 876
BsaHI Gr'CG_yC
Cuts at: 0 497 573 666 689 1157 1230
Size: 497 76 93 23 468 73
Fragments arranged by size:
497 468 93 76 73 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 184 214 721 751 1230
Size: 19 30 135 30 507 30 479
Fragments arranged by size:
507 479 135 30 30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 306 1230
Size: 306 924
BseYI C'CCAG_C
Cuts at: 0 1128 1230
Size: 1128 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 732 928 1230
Size: 78 654 196 302
Fragments arranged by size:
654 302 196 78
BsiEI CG_ry'CG
Cuts at: 0 603 1230
Size: 603 627
BsiHKAI G_wGCw'C
Cuts at: 0 755 1230
Size: 755 475
BsmBI CGTCTCn'nnnn_
Cuts at: 0 433 510 1164 1230
Size: 433 77 654 66
Fragments arranged by size:
654 433 77 66
Bsp1286I G_dGCh'C
Cuts at: 0 24 119 629 755 1029 1191 1230
Size: 24 95 510 126 274 162 39
Fragments arranged by size:
510 274 162 126 95 39 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 298 1230
Size: 298 932
BsrBI CCG'CTC
Cuts at: 0 570 1230
Size: 570 660
BsrFI r'CCGG_y
Cuts at: 0 740 942 1230
Size: 740 202 288
Fragments arranged by size:
740 288 202
BssHII G'CGCG_C
Cuts at: 0 618 1230
Size: 618 612
BssSI C'ACGA_G
Cuts at: 0 193 1230
Size: 193 1037
BstEII G'GTnAC_C
Cuts at: 0 846 1230
Size: 846 384
BstXI CCAn_nnnn'nTGG
Cuts at: 0 246 1230
Size: 246 984
BstYI r'GATC_y
Cuts at: 0 1164 1230
Size: 1164 66
Bsu36I CC'TnA_GG
Cuts at: 0 1016 1055 1230
Size: 1016 39 175
Fragments arranged by size:
1016 175 39
BtgI C'CryG_G
Cuts at: 0 922 1230
Size: 922 308
BtsI GCAGTG_nn'
Cuts at: 0 120 1230
Size: 120 1110
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 335 370 1230
Size: 335 35 860
Fragments arranged by size:
860 335 35
DraIII CAC_nnn'GTG
Cuts at: 0 846 1230
Size: 846 384
DrdI GACnn_nn'nnGTC
Cuts at: 0 502 1069 1230
Size: 502 567 161
Fragments arranged by size:
567 502 161
EaeI y'GGCC_r
Cuts at: 0 401 1230
Size: 401 829
EarI CTCTTCn'nnn_
Cuts at: 0 332 595 1230
Size: 332 263 635
Fragments arranged by size:
635 332 263
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 621 808 1230
Size: 621 187 422
Fragments arranged by size:
621 422 187
EcoICRI GAG'CTC
Cuts at: 0 753 1230
Size: 753 477
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 119 290 313 586 769 882 1230
Size: 65 54 171 23 273 183 113 348
Fragments arranged by size:
348 273 183 171 113 65 54 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 223 1230
Size: 223 1007
EcoO109I rG'GnC_Cy
Cuts at: 0 20 21 116 281 732 984 1026 1078
Size: 20 1 95 165 451 252 42 52
Cuts at: 1078 1230
Size: 152
Fragments arranged by size:
451 252 165 152 95 52 42 20
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 175 1230
Size: 175 1055
HaeII r_GCGC'y
Cuts at: 0 817 919 1230
Size: 817 102 311
Fragments arranged by size:
817 311 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 19 51 656 688 995 1027 1156 1188
Size: 19 32 605 32 307 32 129 32
Cuts at: 1188 1230
Size: 42
Fragments arranged by size:
605 307 129 42 32 32 32 32
19
HincII GTy'rAC
Cuts at: 0 255 1230
Size: 255 975
KpnI G_GTAC'C
Cuts at: 0 450 1230
Size: 450 780
MluI A'CGCG_T
Cuts at: 0 455 1230
Size: 455 775
MslI CAynn'nnrTG
Cuts at: 0 676 1115 1230
Size: 676 439 115
Fragments arranged by size:
676 439 115
MspA1I CmG'CkG
Cuts at: 0 564 659 912 924 969 1230
Size: 564 95 253 12 45 261
Fragments arranged by size:
564 261 253 95 45 12
NaeI GCC'GGC
Cuts at: 0 742 1230
Size: 742 488
NgoMIV G'CCGG_C
Cuts at: 0 740 1230
Size: 740 490
NruI TCG'CGA
Cuts at: 0 1176 1230
Size: 1176 54
PfoI T'CCnGG_A
Cuts at: 0 593 725 1230
Size: 593 132 505
Fragments arranged by size:
593 505 132
PmlI CAC'GTG
Cuts at: 0 354 1230
Size: 354 876
PpuMI rG'GwC_Cy
Cuts at: 0 984 1230
Size: 984 246
PspOMI G'GGCC_C
Cuts at: 0 20 115 1025 1230
Size: 20 95 910 205
Fragments arranged by size:
910 205 95 20
PstI C_TGCA'G
Cuts at: 0 974 1230
Size: 974 256
PvuII CAG'CTG
Cuts at: 0 564 912 969 1230
Size: 564 348 57 261
Fragments arranged by size:
564 348 261 57
SacI G_AGCT'C
Cuts at: 0 755 1230
Size: 755 475
SacII CC_GC'GG
Cuts at: 0 925 1230
Size: 925 305
SapI GCTCTTCn'nnn_
Cuts at: 0 595 1230
Size: 595 635
ScaI AGT'ACT
Cuts at: 0 199 1230
Size: 199 1031
SexAI A'CCwGG_T
Cuts at: 0 50 842 1230
Size: 50 792 388
Fragments arranged by size:
792 388 50
SfcI C'TryA_G
Cuts at: 0 183 510 970 1182 1230
Size: 183 327 460 212 48
Fragments arranged by size:
460 327 212 183 48
StuI AGG'CCT
Cuts at: 0 63 1230
Size: 63 1167
StyI C'CwwG_G
Cuts at: 0 68 1217 1230
Size: 68 1149 13
Fragments arranged by size:
1149 68 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1057 1230
Size: 1057 173
TatI w'GTAC_w
Cuts at: 0 197 1230
Size: 197 1033
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 184 216 721 753 1230
Size: 184 32 505 32 477
Fragments arranged by size:
505 477 184 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 457 1230
Size: 457 773
ZraI GAC'GTC
Cuts at: 0 1158 1230
Size: 1158 72
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AleI AloI AlwNI
ApaI AvaI BanI BanII BbsI BbvCI BlpI Bme1580I
BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI
BssHII BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII KpnI MluI
MslI MspA1I NaeI NgoMIV NruI PfoI PmlI PpuMI
PspOMI PstI PvuII SacI SacII SapI ScaI SexAI
SfcI StuI StyI TaqII TatI TstI XcmI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AlfI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI
BmtI BplI BpuEI BsaBI BsaWI BsiWI BsmI BspEI
BspHI BsrDI BsrGI BstAPI BstBI BstZ17I BtgZI ClaI
DraI EagI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KasI MfeI MmeI MscI NarI NcoI NdeI
NheI NotI NsiI NspI PacI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI XmnI