Restriction map for
pFN21AB7765
(Linear) (Six-base) MAPSORT of: KIBB7765.seq Check: 381 from: 1 to: 1350
KIBB7765.orf
With 182 enzymes: SgfI *
July 3, 2012 16:29 ..
AccI GT'mk_AC
Cuts at: 0 539 1350
Size: 539 811
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1350
Size: 755 595
AlwNI CAG_nnn'CTG
Cuts at: 0 198 552 682 733 1071 1350
Size: 198 354 130 51 338 279
Fragments arranged by size:
354 338 279 198 130 51
ApoI r'AATT_y
Cuts at: 0 970 1081 1350
Size: 970 111 269
Fragments arranged by size:
970 269 111
BanI G'GyrC_C
Cuts at: 0 279 356 1350
Size: 279 77 994
Fragments arranged by size:
994 279 77
BanII G_rGCy'C
Cuts at: 0 500 1350
Size: 500 850
BbeI G_GCGC'C
Cuts at: 0 283 360 1350
Size: 283 77 990
Fragments arranged by size:
990 283 77
BbsI GAAGACnn'nnnn_
Cuts at: 0 660 1350
Size: 660 690
BbvCI CC'TCA_GC
Cuts at: 0 700 1350
Size: 700 650
BglI GCCn_nnn'nGGC
Cuts at: 0 365 1350
Size: 365 985
BmrI ACTGGGnnnn_n'
Cuts at: 0 204 793 1132 1350
Size: 204 589 339 218
Fragments arranged by size:
589 339 218 204
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 811 1350
Size: 811 539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 412 1310 1345 1350
Size: 167 245 898 35 5
Fragments arranged by size:
898 245 167 35 5
Bpu10I CC'TnA_GC
Cuts at: 0 700 1350
Size: 700 650
BsaI GGTCTCn'nnnn_
Cuts at: 0 1286 1350
Size: 1286 64
BsaHI Gr'CG_yC
Cuts at: 0 280 357 1350
Size: 280 77 993
Fragments arranged by size:
993 280 77
BsaWI w'CCGG_w
Cuts at: 0 1106 1350
Size: 1106 244
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 82 112 418 448 1350
Size: 82 30 306 30 902
Fragments arranged by size:
902 306 82 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 87 688 1350
Size: 87 601 662
Fragments arranged by size:
662 601 87
BseYI C'CCAG_C
Cuts at: 0 1064 1350
Size: 1064 286
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 544 550 1350
Size: 253 291 6 800
Fragments arranged by size:
800 291 253 6
BsiEI CG_ry'CG
Cuts at: 0 218 315 365 872 1350
Size: 218 97 50 507 478
Fragments arranged by size:
507 478 218 97 50
BsiWI C'GTAC_G
Cuts at: 0 245 1350
Size: 245 1105
BsmI GAATG_Cn'
Cuts at: 0 769 1350
Size: 769 581
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1141 1350
Size: 1141 209
Bsp1286I G_dGCh'C
Cuts at: 0 500 1350
Size: 500 850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 449 1350
Size: 449 901
BsrBI CCG'CTC
Cuts at: 0 334 434 889 1350
Size: 334 100 455 461
Fragments arranged by size:
461 455 334 100
BsrDI GCAATG_nn'
Cuts at: 0 75 1350
Size: 75 1275
BsrFI r'CCGG_y
Cuts at: 0 276 364 824 1350
Size: 276 88 460 526
Fragments arranged by size:
526 460 276 88
BstAPI GCAn_nnn'nTGC
Cuts at: 0 555 1350
Size: 555 795
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1198 1350
Size: 1198 152
BtgI C'CryG_G
Cuts at: 0 79 351 473 1116 1191 1220 1350
Size: 79 272 122 643 75 29 130
Fragments arranged by size:
643 272 130 122 79 75 29
EaeI y'GGCC_r
Cuts at: 0 215 312 362 482 869 1350
Size: 215 97 50 120 387 481
Fragments arranged by size:
481 387 215 120 97 50
EagI C'GGCC_G
Cuts at: 0 215 312 362 869 1350
Size: 215 97 50 507 481
Fragments arranged by size:
507 481 215 97 50
EarI CTCTTCn'nnn_
Cuts at: 0 29 795 1350
Size: 29 766 555
Fragments arranged by size:
766 555 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 90 894 1350
Size: 90 804 456
Fragments arranged by size:
804 456 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 412 755 1310 1345 1350
Size: 167 245 343 555 35 5
Fragments arranged by size:
555 343 245 167 35 5
EcoO109I rG'GnC_Cy
Cuts at: 0 84 586 1350
Size: 84 502 764
Fragments arranged by size:
764 502 84
EcoRI G'AATT_C
Cuts at: 0 1081 1350
Size: 1081 269
FseI GG_CCGG'CC
Cuts at: 0 368 1350
Size: 368 982
HaeII r_GCGC'y
Cuts at: 0 283 360 413 1350
Size: 283 77 53 937
Fragments arranged by size:
937 283 77 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 211 243 680 712 1350
Size: 211 32 437 32 638
Fragments arranged by size:
638 437 211 32 32
HincII GTy'rAC
Cuts at: 0 540 1350
Size: 540 810
KasI G'GCGC_C
Cuts at: 0 279 356 1350
Size: 279 77 994
Fragments arranged by size:
994 279 77
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 824 1350
Size: 461 363 526
Fragments arranged by size:
526 461 363
MslI CAynn'nnrTG
Cuts at: 0 595 762 1350
Size: 595 167 588
Fragments arranged by size:
595 588 167
MspA1I CmG'CkG
Cuts at: 0 81 198 475 1222 1306 1350
Size: 81 117 277 747 84 44
Fragments arranged by size:
747 277 117 84 81 44
NaeI GCC'GGC
Cuts at: 0 278 366 1350
Size: 278 88 984
Fragments arranged by size:
984 278 88
NarI GG'CG_CC
Cuts at: 0 280 357 1350
Size: 280 77 993
Fragments arranged by size:
993 280 77
NcoI C'CATG_G
Cuts at: 0 1116 1350
Size: 1116 234
NgoMIV G'CCGG_C
Cuts at: 0 276 364 1350
Size: 276 88 986
Fragments arranged by size:
986 276 88
NspI r_CATG'y
Cuts at: 0 510 1053 1350
Size: 510 543 297
Fragments arranged by size:
543 510 297
PflMI CCAn_nnn'nTGG
Cuts at: 0 198 1350
Size: 198 1152
PpuMI rG'GwC_Cy
Cuts at: 0 84 586 1350
Size: 84 502 764
Fragments arranged by size:
764 502 84
PshAI GACnn'nnGTC
Cuts at: 0 1298 1350
Size: 1298 52
PstI C_TGCA'G
Cuts at: 0 1238 1350
Size: 1238 112
PvuII CAG'CTG
Cuts at: 0 198 1306 1350
Size: 198 1108 44
Fragments arranged by size:
1108 198 44
SacII CC_GC'GG
Cuts at: 0 82 476 1223 1350
Size: 82 394 747 127
Fragments arranged by size:
747 394 127 82
SalI G'TCGA_C
Cuts at: 0 538 1350
Size: 538 812
SanDI GG'GwC_CC
Cuts at: 0 84 1350
Size: 84 1266
SapI GCTCTTCn'nnn_
Cuts at: 0 795 1350
Size: 795 555
SfcI C'TryA_G
Cuts at: 0 117 1234 1350
Size: 117 1117 116
Fragments arranged by size:
1117 117 116
SfoI GGC'GCC
Cuts at: 0 281 358 1350
Size: 281 77 992
Fragments arranged by size:
992 281 77
SspI AAT'ATT
Cuts at: 0 997 1350
Size: 997 353
StuI AGG'CCT
Cuts at: 0 304 1350
Size: 304 1046
StyI C'CwwG_G
Cuts at: 0 1116 1350
Size: 1116 234
TatI w'GTAC_w
Cuts at: 0 738 1350
Size: 738 612
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 82 114 1350
Size: 82 32 1236
Fragments arranged by size:
1236 82 32
XmnI GAAnn'nnTTC
Cuts at: 0 929 1350
Size: 929 421
Enzymes that do cut:
AccI AcuI AlwNI ApoI BanI BanII BbeI BbsI
BbvCI BglI BmrI BplI BpmI Bpu10I BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstXI
BtgI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FseI HaeII Hin4I HincII KasI MmeI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PflMI PpuMI PshAI
PstI PvuII SacII SalI SanDI SapI SfcI SfoI
SspI StuI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmtI BpuEI BsaAI
BsaBI BsiHKAI BspEI BspHI BsrGI BssHII BssSI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EcoICRI EcoNI EcoRV FalI FspI
FspAI HindIII HpaI KpnI MfeI MluI MscI NdeI
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI ZraI