Restriction map for
pFN21AB7764
(Linear) (Six-base) MAPSORT of: KIBB7764.seq Check: 5474 from: 1 to: 1503
KIBB7764.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1310 1503
Size: 1310 193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 445 1503
Size: 304 141 1058
Fragments arranged by size:
1058 304 141
AgeI A'CCGG_T
Cuts at: 0 915 1503
Size: 915 588
AleI CACnn'nnGTG
Cuts at: 0 517 1503
Size: 517 986
ApaI G_GGCC'C
Cuts at: 0 96 1503
Size: 96 1407
AvaI C'yCGr_G
Cuts at: 0 57 95 135 295 697 1503
Size: 57 38 40 160 402 806
Fragments arranged by size:
806 402 160 57 40 38
AvrII C'CTAG_G
Cuts at: 0 837 1503
Size: 837 666
BanI G'GyrC_C
Cuts at: 0 109 174 220 548 579 1503
Size: 109 65 46 328 31 924
Fragments arranged by size:
924 328 109 65 46 31
BanII G_rGCy'C
Cuts at: 0 96 698 1288 1370 1503
Size: 96 602 590 82 133
Fragments arranged by size:
602 590 133 96 82
BbeI G_GCGC'C
Cuts at: 0 178 224 552 583 1503
Size: 178 46 328 31 920
Fragments arranged by size:
920 328 178 46 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 644 1503
Size: 644 859
BciVI GTATCCnnnnn_n'
Cuts at: 0 13 1503
Size: 13 1490
BglI GCCn_nnn'nGGC
Cuts at: 0 196 1449 1503
Size: 196 1253 54
Fragments arranged by size:
1253 196 54
BglII A'GATC_T
Cuts at: 0 1043 1503
Size: 1043 460
Bme1580I G_kGCm'C
Cuts at: 0 96 1445 1503
Size: 96 1349 58
Fragments arranged by size:
1349 96 58
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 947 1503
Size: 947 556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 917 1503
Size: 703 214 586
Fragments arranged by size:
703 586 214
Bpu10I CC'TnA_GC
Cuts at: 0 797 1503
Size: 797 706
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1405 1503
Size: 538 867 98
Fragments arranged by size:
867 538 98
BsaI GGTCTCn'nnnn_
Cuts at: 0 1118 1197 1503
Size: 1118 79 306
Fragments arranged by size:
1118 306 79
BsaHI Gr'CG_yC
Cuts at: 0 175 221 549 580 1469 1503
Size: 175 46 328 31 889 34
Fragments arranged by size:
889 328 175 46 34 31
BsaWI w'CCGG_w
Cuts at: 0 915 1503
Size: 915 588
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1258 1288 1399 1429 1503
Size: 1258 30 111 30 74
Fragments arranged by size:
1258 111 74 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 616 646 661 670 700 703 706 721
Size: 616 30 15 9 30 3 3 15
Cuts at: 721 724 769 1485 1503
Size: 3 45 716 18
Fragments arranged by size:
716 616 45 30 30 18 15 15
9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 11 15 264 811 1203 1281 1305 1503
Size: 11 4 249 547 392 78 24 198
Fragments arranged by size:
547 392 249 198 78 24 11 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 1503
Size: 322 1181
BsiEI CG_ry'CG
Cuts at: 0 191 200 314 1133 1163 1234 1503
Size: 191 9 114 819 30 71 269
Fragments arranged by size:
819 269 191 114 71 30 9
BsiHKAI G_wGCw'C
Cuts at: 0 158 415 446 698 1503
Size: 158 257 31 252 805
Fragments arranged by size:
805 257 252 158 31
BsmBI CGTCTCn'nnnn_
Cuts at: 0 971 1503
Size: 971 532
Bsp1286I G_dGCh'C
Cuts at: 0 96 158 415 446 698 1288 1370 1445
Size: 96 62 257 31 252 590 82 75
Cuts at: 1445 1503
Size: 58
Fragments arranged by size:
590 257 252 96 82 75 62 58
31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1310 1503
Size: 1310 193
BsrBI CCG'CTC
Cuts at: 0 1091 1180 1272 1503
Size: 1091 89 92 231
Fragments arranged by size:
1091 231 92 89
BsrFI r'CCGG_y
Cuts at: 0 545 905 915 1229 1503
Size: 545 360 10 314 274
Fragments arranged by size:
545 360 314 274 10
BssHII G'CGCG_C
Cuts at: 0 269 541 820 1503
Size: 269 272 279 683
Fragments arranged by size:
683 279 272 269
BstYI r'GATC_y
Cuts at: 0 1043 1503
Size: 1043 460
BtgI C'CryG_G
Cuts at: 0 185 239 314 953 1322 1503
Size: 185 54 75 639 369 181
Fragments arranged by size:
639 369 185 181 75 54
ClaI AT'CG_AT
Cuts at: 0 761 1503
Size: 761 742
EaeI y'GGCC_r
Cuts at: 0 188 197 311 360 488 785 907 950
Size: 188 9 114 49 128 297 122 43
Cuts at: 950 1160 1231 1357 1503
Size: 210 71 126 146
Fragments arranged by size:
297 210 188 146 128 126 122 114
71 49 43 9
EagI C'GGCC_G
Cuts at: 0 188 197 311 1160 1231 1503
Size: 188 9 114 849 71 272
Fragments arranged by size:
849 272 188 114 71 9
EarI CTCTTCn'nnn_
Cuts at: 0 638 1503
Size: 638 865
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 1014 1131 1391 1503
Size: 22 992 117 260 112
Fragments arranged by size:
992 260 117 112 22
EcoICRI GAG'CTC
Cuts at: 0 696 1503
Size: 696 807
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 445 703 917 1503
Size: 304 141 258 214 586
Fragments arranged by size:
586 304 258 214 141
EcoNI CCTnn'n_nnAGG
Cuts at: 0 514 556 1503
Size: 514 42 947
Fragments arranged by size:
947 514 42
EcoO109I rG'GnC_Cy
Cuts at: 0 92 140 378 729 847 1503
Size: 92 48 238 351 118 656
Fragments arranged by size:
656 351 238 118 92 48
HaeII r_GCGC'y
Cuts at: 0 57 178 224 552 583 940 1201 1503
Size: 57 121 46 328 31 357 261 302
Fragments arranged by size:
357 328 302 261 121 57 46 31
KasI G'GCGC_C
Cuts at: 0 174 220 548 579 1503
Size: 174 46 328 31 924
Fragments arranged by size:
924 328 174 46 31
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 1503
Size: 173 1330
MscI TGG'CCA
Cuts at: 0 362 490 1503
Size: 362 128 1013
Fragments arranged by size:
1013 362 128
MslI CAynn'nnrTG
Cuts at: 0 502 517 1503
Size: 502 15 986
Fragments arranged by size:
986 502 15
MspA1I CmG'CkG
Cuts at: 0 15 67 77 187 193 241 264 316
Size: 15 52 10 110 6 48 23 52
Cuts at: 316 1129 1203 1324 1363 1503
Size: 813 74 121 39 140
Fragments arranged by size:
813 140 121 110 74 52 52 48
39 23 15 10 6
NaeI GCC'GGC
Cuts at: 0 547 1503
Size: 547 956
NarI GG'CG_CC
Cuts at: 0 175 221 549 580 1503
Size: 175 46 328 31 923
Fragments arranged by size:
923 328 175 46 31
NgoMIV G'CCGG_C
Cuts at: 0 545 1503
Size: 545 958
NotI GC'GGCC_GC
Cuts at: 0 188 197 1503
Size: 188 9 1306
Fragments arranged by size:
1306 188 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 1046 1503
Size: 1046 457
PfoI T'CCnGG_A
Cuts at: 0 1314 1503
Size: 1314 189
PpuMI rG'GwC_Cy
Cuts at: 0 140 378 847 1503
Size: 140 238 469 656
Fragments arranged by size:
656 469 238 140
PspOMI G'GGCC_C
Cuts at: 0 92 1503
Size: 92 1411
PstI C_TGCA'G
Cuts at: 0 1034 1151 1503
Size: 1034 117 352
Fragments arranged by size:
1034 352 117
PvuII CAG'CTG
Cuts at: 0 15 264 1503
Size: 15 249 1239
Fragments arranged by size:
1239 249 15
SacI G_AGCT'C
Cuts at: 0 698 1503
Size: 698 805
SacII CC_GC'GG
Cuts at: 0 188 242 317 1325 1503
Size: 188 54 75 1008 178
Fragments arranged by size:
1008 188 178 75 54
SanDI GG'GwC_CC
Cuts at: 0 140 378 847 1503
Size: 140 238 469 656
Fragments arranged by size:
656 469 238 140
SexAI A'CCwGG_T
Cuts at: 0 526 1503
Size: 526 977
SfcI C'TryA_G
Cuts at: 0 1030 1147 1389 1503
Size: 1030 117 242 114
Fragments arranged by size:
1030 242 117 114
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 196 1503
Size: 196 1307
SfoI GGC'GCC
Cuts at: 0 176 222 550 581 1503
Size: 176 46 328 31 922
Fragments arranged by size:
922 328 176 46 31
SgrAI Cr'CCGG_yG
Cuts at: 0 545 1503
Size: 545 958
SmaI CCC'GGG
Cuts at: 0 59 97 297 1503
Size: 59 38 200 1206
Fragments arranged by size:
1206 200 59 38
SmlI C'TyrA_G
Cuts at: 0 553 1420 1503
Size: 553 867 83
Fragments arranged by size:
867 553 83
StuI AGG'CCT
Cuts at: 0 430 842 1503
Size: 430 412 661
Fragments arranged by size:
661 430 412
StyI C'CwwG_G
Cuts at: 0 210 467 837 1397 1503
Size: 210 257 370 560 106
Fragments arranged by size:
560 370 257 210 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 97 529 1503
Size: 97 432 974
Fragments arranged by size:
974 432 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 902 934 1258 1290 1503
Size: 902 32 324 32 213
Fragments arranged by size:
902 324 213 32 32
XmaI C'CCGG_G
Cuts at: 0 57 95 295 1503
Size: 57 38 200 1208
Fragments arranged by size:
1208 200 57 38
Enzymes that do cut:
AarI AcuI AgeI AleI ApaI AvaI AvrII BanI
BanII BbeI BbsI BciVI BglI BglII Bme1580I BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BstYI BtgI ClaI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI
SexAI SfcI SfiI SfoI SgrAI SmaI SmlI StuI
StyI TaqII TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
BaeI BamHI BbvCI BcgI BclI BfrBI BlpI BmgBI
BmrI BmtI BsaAI BsaBI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI CspCI DraI DraIII DrdI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI
SbfI ScaI SgfI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XcmI XhoI XmnI ZraI